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Morya, V. K.,Son, M.,Lee, H. B.,Kim, E. k. HUMANA PRESS 2014 Molecular biotechnology Vol.56 No.3
Melanin synthesis is a complex phenomenon which involves about 192 known gene products. Among them, MITF is a key transcription factor for tyrosinase, Trp1 and Trp2 proteins, which are essential for melanin biosynthesis. Thus, intervening inhibitor for the MITF-E-box complex formation can downregulate melanin synthesis. The focus of the present study is to develop a surface plasmon resonance-based system to screen the MITF-E-box complex inhibitor. The standardization of the MITF and E-box binding assay was calibrated for kinetics and specificity, in the presence of a pre-incubated 22 mer sequence containing mutated E-box (CTTGAG) along MITF. The binding assay with C17 was optimized and the steady-state kinetics was evaluated. C17 was identified as inhibitor to MITF-E-box, by virtual screening followed by in vitro assessment and EMSA assay. The k (a) and k (d) were found to be 5.5 x 103 M-1 s(-1) and 0.0014 s(-1), respectively, while the steady-state association constant (K (A)) was 3.928 x 106 M-1. The resonance variations after inhibition were quantified and analyzed to develop the standard method for screening of microphthalmia transcription factor-E-box binding inhibitor.
Algal fucoidan: structural and size-dependent bioactivities and their perspectives.
Morya, V K,Kim, Jungeun,Kim, Eun-Ki Springer International 2012 Applied microbiology and biotechnology Vol.93 No.1
<P>Fucoidan is a complex-sulfated polysaccharide distributed in various marine organisms, and the brown algae are reported as the major producer. The fucoidan is important for their high bioactive properties, like antibacterial, anticoagulant, antiviral, anti-tumor, etc., and many more to be explored. There is a strong archival support for the bioactivity and promising properties of this molecule, which creates a hope for this molecule as future drug against thrombosis and some kind of cancers. Reports other than the above bioactive properties have also been a matter of interest for the design of signal or enzyme-arrested new class of drugs. In the past three decades, the research on isolation, molecular characterization, and screening of biological applications has significantly increased. One major issue associated with this molecule is the higher size and seasonal variation in their chemical composition; to resolve the issue and maintain its bioactivity, a prioritized and literal hydrolysis process is required to be developed. Here, in this mini-review, we have tried to summarize the algal fucoidan research and the bioactivities influenced by their molecular size.</P>
Production and characterization of low molecular weight sophorolipid under fed-batch culture
Morya, V.K.,Park, J.h.,Kim, T.J.,Jeon, S.,Kim, E.K. Elsevier Applied Science 2013 Bioresource technology Vol.143 No.-
The present study was designed for the production and optimization of the C12-C14 sophorolipid, using the yeast Candida bombicola ATCC-22214. The fermentation was carried under fed-batch culture conditions i.e., maintaining 15% coconut oil and 10% glucose as hydrophobic and hydrophilic carbon sources, respectively. A maximum yield 54.0g/L (in 234h) was achieved. A significant antimicrobial activity, surface activity, and emulsion ability were recorded. The native sophorolipid was found as enhancer of detergent efficacy of commercial detergent, tested on complex, smudge and oil contaminated clothes. Molecular weight of the C12 (605/623) and C14 (633/651) sophorolipids were determined by LC-MS which revealed it as diacetylated sophorolipid. This study is being important in terms of yield, which is better than the previously reported.
Ionic liquid assisted recovery of cobalt and nickel metals from spent lithium-ion batteries (LIBs)
( Raj Tirath ),( Raj Morya ),( Ashutosh Kumar Pandey ),( Sang-hyoun Kim ) 한국폐기물자원순환학회 2022 ISSE 초록집 Vol.2022 No.-
Lithium-ion batteries (LIBs) have become an essential component of the energy supply chain for transportation (in electric vehicles) and renewable energy storage systems. This surge in demand necessitates an increase in production, which, in turn, results in a large number of waste LIBs. LIB cathode material primarily contains heavy metal elements such as nickel (Ni) and cobalt (Co), which are potentially hazardous to human health and the environment if discarded improperly. The leaching of Co and Ni into various water streams has become an environmental hazard and is continuously affecting human health through the food chain. Thus, recycling spent LIBs has gotten a lot of attention because it provides a cost-effective way to ensure a steady supply of these metals for LIB remanufacturing. Thus, recycling spent LIBs has received a lot of attention because it offers a cost-effective way to ensure a steady supply of these metals for LIB remanufacturing while also encouraging safer environmental load reduction. Solvent extraction is the most widely accepted method for separating these metals, but traditional extractants employed in conjunction with molecular diluents often lack selectivity and cause major environmental hurdles. Thus, the present study demonstrated a state-of-the-art approach for the recovery of cobalt and nickel from waste LIBs using green ionic liquids, which is such as recycling from a sustainable perspective. Ionic liquids are molten salts of organic cation and inorganic/ organic anion and are considered as green solvents. Here in, two halogen-free, low viscous, biocompatible fatty acid-based hydrophobic ionic liquids (ILs) were synthesized, characterized, and used to recover cobalt, Co(II), and nickel, Ni(II), from aqueous solutions. The extraction behaviors of Co(II) and Ni(II) were studied further by varying the equilibrium time, ILs molar concentration, metal loading, and temperature. Thermodynamic parameters such as enthalpy change and Gibbs free energy change were also investigated showed that metal recovery is governed by metal transfer phenomenon. Study revealed that fatty acid-based ILs were found to be capable of extracting > 99 % Co(II) and Ni(II) from aqueous solutions at 298 K in 15 minutes using a 1:1 (org: aq.) ratio at low concentrations of 2.5 to 10 g L<sup>-1</sup>. Furthermore, when the metal concentration was greater than 10 g L<sup>-1</sup>, Co(II) extraction was preferred over Ni(II) extraction for methyltrioctylammonium oleate IL.