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김주혁(Ju Hyeok Kim),정영훈(Young Hoon Jeong),이미선(Mi Sun Lee),김문교(Moonkyo Kim),조남수(Namsu Jo),길진수(Jinsu Gil),구성철(Sung Cheol Koo),이이(Yi Lee) 한국약용작물학회 2023 한국약용작물학회지 Vol.31 No.1
Background: Three medicinal plants of the Campanulaceae, Codonopsis lanceolata, C. pilosula, and C. ussuriensis are perennial vines of the genus Codonopsis, and are widely disturbuted in East Asia. C. lanceolata, C. pilosula, and C. ussuriensis have been used as medicine to alleviate inflammation, increase red blood cell count and hemoglobin levels, and improve spleen and stomach functions, respectively. The three species have similar morphologies, although their pharmacological effects differ. Methods and Results: We identified polymorphic loci by comparing chloroplast and mitochondrial sequences of C. lanceolata, C. pilosula, and C. ussuriensis. We designed primers based on these polymorphic loci and conducted polymerase chain reaction (PCR) analysis to verify the polymorphisms. Three insertion–deletion (InDel) markers were ultimately developed: CD-mt-02, which identified C. pilosula; CD-mt-06, which identified C. ussuriensis; and CD-cp-07, which identified all the three species. Conclusions: The three InDel markers developed in this study easily distinguished the three plant species based on a simple PCR test.