Changes in spatial variations of sap flow in Korean pine trees due to environmental factors and their effects on estimates of stand transpiration

Moon, M.,Kim, T.,Park, J.,Cho, S.,Ryu, D.,Suh, S.,Kim, H. S. Springer Science + Business Media 2016 Journal of mountain science Vol.13 No.6

<P>It is difficult to scale up measurements of the sap flux density (J(S)) for the characterization of tree or stand transpiration (E) due to spatial variations in J(S) and their temporal changes. To assess spatial variations in the sap flux density of Korean pine (Pinus koraiensis) and their effects on E estimates, we measured the J(S) using Granier-type sensors. Within trees, the J(S) decreased exponentially with the radial depth, and the J(S) of the east aspects were higher than those of the west aspects. Among trees, there was a positive relationship between J(S) and the tree diameter at breast height, and this positive relationship became stronger as the transpiration demand increased. The spatial variations that caused large errors in E estimates (i.e., up to 110.8 % when radial variation was ignored) had varied systematically with environmental factors systematic characteristics in relation to environmental factors. However, changes in these variations did not generate substantial errors in the E estimates. For our study periods, the differences in the daily E (E-D) calculated by ignoring radial, azimuthal and tree-to-tree variations and the measured ED were fairly constant, especially when the daily vapor pressure deficit (D_(D)) was higher than 0.6 kPa. These results imply that the effect of spatial variations changes on sap flow can be a minor source of error compared with spatial variations (radial, azimuthal and tree-to-tree variations) when considering E estimates.</P>

IL-32γ inhibits cancer cell growth through inactivation of NF-κB and STAT3 signals

Oh, J H,Cho, M-C,Kim, J-H,Lee, S Y,Kim, H J,Park, E S,Ban, J O,Kang, J-W,Lee, D-H,Shim, J-H,Han, S B,Moon, D C,Park, Y H,Yu, D-Y,Kim, J-M,Kim, S H,Yoon, D-Y,Hong, J T Nature Publishing Group 2011 Oncogene Vol.30 No.30

<P>Several studies have shown physiological functions of interleukin (IL)-32, a novel cytokine. However, the role of IL-32 in cancer development has not been reported. In this study, we showed that IL-32γ inhibited tumor growth in IL-32γ-overexpressing transgenic mice inoculated with melanoma as well as colon tumor growth in xenograft nude mice inoculated with IL-32γ-transfected colon cancer cells (SW620). The inhibitory effect of IL-32γ on tumor growth was associated with the inhibition of constitutive activated nuclear transcription factor-κB (NF-κB) and of signal transducer and activator of transcription 3 (STAT3). The expression of antiapoptotic, cell proliferation and tumor-promoting genes (<I>bcl-2</I>, <I>X-chromosome inhibitor of apoptosis protein</I> (<I>IAP</I>), <I>cellular IAP</I> and <I>cellular FADD-like IL-1β-converting enzyme-inhibitory protein</I>, <I>cyclin D</I>), cyclin-dependent kinase 4, cycolooxygenase-2 and inducible nitric oxide synthase was decreased, whereas the expression of apoptotic target genes (<I>caspase-3</I> and <I>-9</I>, <I>bax</I>) increased. In tumor, spleen and blood, the number of cytotoxic CD8<SUP>+</SUP> T cells and CD57<SUP>+</SUP> natural killer cells and the levels of IL-10 increased, but that of tumor necrosis factor-α (TNF-α), IL-1β and IL-6 decreased. We also found that forced overexpression of IL-32γ inhibited colon cancer cell (SW620 and HCT116) growth accompanied with the inhibition of activated NF-κB and STAT3 <I>in vitro</I>. In addition, when IL-32γ was knocked down by small interfering RNA (siRNA) or neutralized with an anti-IL-32γ antibody, IL-32γ-induced colon cancer cell growth inhibition, the IL-32γ-induced decrease of TNF-α, IL-1 and IL-6 production, and the increase of IL-10 production were abolished. However, siRNA of NF-κB and STAT3 augmented IL-32γ-induced colon cancer cell growth inhibition. These findings indicate significant pathophysiological roles of IL-32γ in cancer development.</P>

Vitamin D-binding protein interacts with Aβ and suppresses Aβ-mediated pathology

Moon, M,Song, H,Hong, H J,Nam, D W,Cha, M-Y,Oh, M S,Yu, J,Ryu, H,Mook-Jung, I Macmillan Publishers Limited 2013 CELL DEATH AND DIFFERENTIATION Vol.20 No.4

The level of vitamin D-binding protein (DBP) is increased in the cerebrospinal fluid of patients with Alzheimer’s disease (AD), suggesting a relationship with its pathogenesis. In this study, we investigated whether and how DBP is related to AD using several different approaches. A pull-down assay and a surface plasmon resonance binding assay indicated direct interactions between purified DBP and amyloid beta (Aβ), which was confirmed in the brain of AD patients and transgenic AD model mice by immunoprecipitation assay and immunohistochemical double-staining method. Moreover, atomic force microscopic examination revealed that DBP reduced Aβ aggregation in vitro. DBP also prevented Aβ-mediated death in cultured mouse hippocampal HT22 cell line. Finally, DBP decreased Aβ-induced synaptic loss in the hippocampus and rescued memory deficits in mice after injection of Aβ into the lateral ventricle. These results provide converging evidence that DBP attenuates the harmful effects of Aβ by a direct interaction, and suggest that DBP is a promising therapeutic agent for the treatment of AD.

Development and Application of Efficacy Evaluation Techniques for Barrier Creams

Suh, D. H 大韓臨床藥理學會 1997 臨床藥理學會誌 Vol.5 No.1

Oils과 Carrageenan을 이용한 저지방 소세지의 저장기간에 따른 pH, 지방산 조성 및 콜레스테롤의 변화

문점동,송또준,박구부,신택순 진주산업대학교 농업기술연구소 1996 農業技術硏究所報 Vol.9 No.-

육제품을 많이 섭취하면 성인병을 유발한다고 믿고 있는 소비자들의 인식 변화를 유도하기 위한 저지방 소세지의 생산 가능성을 제시코자 첨가하는 지방의 량을 줄여 이에 대체물로서 물을 다량 첨가함과 동시에 3종의 oil로 일부를 대체하고 유화안정제로 카라기난을 첨가하여 low-fat sausage를 제조한 후 5℃에서 6주간 저장하면서 저장기간에 따른 pH, 지방산 조성 및 콜레스테롤의 변화에 대한 실험결과를 요약하면 다음과 같다. 1. 모든 처리구들의 pH는 저장기간이 경과함에 따라서 증가하였으며, 저지방 첨가구의 대조구인 B구의 pH에 비하여 카라기난을 첨가하지 않은 저지방 처리구들의 pH는 유의적인 차이를 나타내지 않았으나 대체로 낮은 pH를 나타내었고, 전 저장기간 동안 카라기난을 첨가한 구들이 대조구 및 다른 저지방 처리구에 비하여 높은 pH를 유지하였다(P<0.05). 2. 지방산의 조성은 대체한 oil에 의해서 oleic, linoleic, linolenic acid가 각각 증가하였으며, 저장기간에 따른 지방산 조성의 변화는 나타나지 않았다. 불포화도는 perilla, sunflower, olive의 순으로 낮았으며, 저장기간에 따른 불포화도의 변화는 나타나지 않았다. 오메가 지방산의 비율은 대체한 oil에 의해서 대조구와는 다른 비율을 나타내었으며 perilla유 대체구가 가장 높았고, 저장기간의 경과에 따른 변화는 없었다. 3. 대조구는 모든 저지방 처리구들 보다 높은 cholesterol 함량을 나타내었고, oil의 대체로 인한 cholesterol 함량의 감소가 나타났으며, 저장기간이 경과함에 따른 cholesterol함량의 변화는 없었다. This study was conducted to investigate the possibility of production of low fat sausage which was made with vegetable oils and added water to reduce back fat content in the sausage. Raw meats for the sausage were removed from the pork carcass(90∼100kg, female, 5∼7months of age) 4∼6 hours after slaughter and randomly assigned to one of eight treatments : control(back fat 30% and water 10), treatment A(back fat 15% and water 25%), treatment B(back fat 7.5%, olive oil 7.5% and water 25%), treatment C(back fat 7.5%, olive oil 7.5%, water 15% and carrageenan 0.5%), treatment D(back fat 7.5%, sunflower oil 7.5% and water 25%), treatment E(back fat 7.5%, sunflower oil 7.5%, water 25% and carrageenan 0.5%), treatment F(back fat 7.5%, perilla oil 7.5% and water 15%) and treatment G(back fat 7.5%, perilla oil 7.5%, water 25% and carrageenan 0.5%). The sausage samples were stored at 5±1℃. The pH, fatty acid and cholesterol were analyzed for over a period of times(0, 2, 4, 6 weeks) The results obtained were summarized as follows : The pH of all treatments was increased with the storage period. The pH values of sausages with carrageenan were lower than that of treatment A but there were no significant differences between the sausages and treatment A. The pH of sausages with carrageenan was higher than those of control and other(P<0.05). The composition of fatty acids of sausages with olive, sunflower and perilla oil was changed, so treatment B and c, D and E and F and G had higher oleic acid, linoleic acid and linolenic acid, respectively. There was no difference with the storage period. The ratio of unsaturated to saturated fatty acids of sausages was affected by oil type and there was no difference with the storage period. The ratio of n-3 to n-6 fatty acids of sausages was different from that of control, and that of treatment F and G, both of which perilla oil was added, was higher than those of others. There was no difference with the storage period. The cholesterol content of control was higher than those of others, and that of low fat sausages was decreased. There was no difference with the storage period.

HWE(Hot Wall Epitaxy)에 의한 ZnIn_2S_4 박막 성장과 광전도 특성

홍광준,이관교,정준우,정경아,방진주,장현규,문종대,김혜숙 조선대학교 기초과학연구소 1999 自然科學硏究 Vol.22 No.1

HWE 방법에 의해 ZnIn_2S_4 박막을 Si(00) 기판 위에 성장시켰다. 증발원과 기판의 온도를 각각 610℃, 450℃로 하여 성장시킨 ZnIn_2S_4 박막의 이중 결정 X-선 요동곡선(DCRC)의 반폭치(FWHM)값이 245 arcsec로 가장 작았다. Van der Pauw 방법으로 Hall 효과를 측정하여 운반자농도의 1n n 대 1/T에서 구한 활성화에너지는 0.17eV로 측정되었다. Hall 이동도의 온도 의존성은 30K에서 100K까지는 불순물산란에 기인하고, 100K에서 293K까지는 격자산란에 기인한것으로 고찰되었다. 광전도셀의 특성으로 spectral response, 최대 허용소비전력(MAPD), 광전류와 암전류(pc/dc)의 비 및 응답시간을 측정하였다. S 증기분위기에서 열처리한 광전도 셀의 경우, 감도(??)는 0.99, pc/dc은 1.37x10^7, 그리고 최대 허용소비전력(MAPD)은 336mW, 오름시간(rise time)은 9ms, 내림시간(decay time)은 9.8ms로 가장 좋은 광전도 특성을 얻었다. The ZnIn_2S_4 thin films were grown on the Si(100) wafers by a hot wall epitaxy method(HWE). The source and substrate temperature are 610℃ and 450℃ respectively. The crystalline structure of epilayers was investigated by double crystal X-ray diffraction(DCXD). Hall effect on the sample was measured by the van der Pauw method and studied on the carrier density and mobility dependence on temperature. From Hall data, the mobility was increased in the temperature range 30K to 100K by impurity scattering and decreased in the temperature range 100K to 293K by the lattice scattering. In order to explore the applicability as a photoconductive cell, we measured the sensitivity(??), the ratio of photocurrent to darkcurrent(pc/dc), maximum allowable power dissipation(MAPD), spectral response and response time. The results indicated that the photoconductive characteristic were the best for the samples annealed in S vapor compare with in Zn, In, air and vacuum vapour. Then we obtained the sensitivity of 0.99, the value of pc/dc of 1.37x10^7, the MAPD of 336mW, and the rise and decay time of 9ms and 9.8ms, respectively.

Requirement for Ras/Raf/ERK pathway in naringin-induced G1-cell-cycle arrest via p21WAF1 expression

Kim, D.-I.,Lee, S.-J.,Lee, S.-B.,Park, K.,Kim, W.-J.,Moon, S.-K. Oxford University Press 2008 Carcinogenesis Vol.29 No.9

<P>Naringin, an active flavonoid found in citrus fruit extracts, has pharmacological utility. The present study identified a novel mechanism of the anticancer effects of naringin in urinary bladder cancer cells. Naringin treatment resulted in significant dose-dependent growth inhibition together with G(1)-phase cell-cycle arrest at a dose of 100 microM (the half maximal inhibitory concentration) in 5637 cells. In addition, naringin treatment strongly induced p21WAF1 expression, independent of the p53 pathway, and downregulated expression of cyclins and cyclin dependent kinases (CDKs). Moreover, treatment with naringin induced phosphorylation of extracellular signal-regulated kinase (ERK), p38 mitogen-activated protein kinase and c-Jun N-terminal kinase. Among the pathways examined, only PD98059, an ERK-specific inhibitor, blocked naringin-dependent p21WAF1 expression. Consistently, blockade of ERK function reversed naringin-mediated inhibition of cell proliferation and decreased cell-cycle proteins. Furthermore, naringin treatment increased both Ras and Raf activation. Transfection of cells with dominant-negative Ras (RasN17) and Raf (RafS621A) mutant genes suppressed naringin-induced ERK activity and p21WAF1 expression. Finally, the naringin-induced reduction in cell proliferation and cell-cycle proteins also was abolished in the presence of RasN17 and RafS621A mutant genes. These data demonstrate that the Ras/Raf/ERK pathway participates in p21WAF1 induction, subsequently leading to a decrease in the levels of cyclin D1/CDK4 and cyclin E-CDK2 complexes and naringin-dependent inhibition of cell growth. Overall, these unexpected findings concerning the molecular mechanisms of naringin in 5637 cancer cells provide a theoretical basis for the therapeutic use of flavonoids to treat malignancies.</P>

기존 교류선로의 직류변환 송전방식에 있어서 선정기법에 관한 연구

김동준(D.J. Kim),문영환(Y.H. Moon),이동일(D.I. Lee),윤재영(J.Y. Yoon),양광호(K.H. Yang) 대한전기학회 2002 대한전기학회 학술대회 논문집 Vol.2002 No.11

Chemical Bath Deposition 방법으로 제작한 CdSe 박막의 특성

홍광준,이상열,유상하,서상석,문종대,신영진,정태수,신현길,김택성,송정훈,유기수 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

Chemical bath deposition 방법으로 다결정 CdSe 박막을 세라믹 기판 위에 성장시킨 다음 온도를 변화시켜 열처리하고 X-선 회절무늬를 측정하여 결정구조를 밝혔다. 450℃로 열처리한 시료가 X-선 회절무늬로 부터 외삽법에 의해 a_o와 c_o 는 각각 4.302 Å과 7.014 Å인 육방정계임을 알았다. 이 때 낱알크기는 약 0.3㎛이었다. Van der Pauw 방법으로 Hall 효과를 측정하여 운반자 농도와 이동도의 온도의존성을 연구하였다. 이동도는 33 K 에서 200 K 까지는 압전산란에 의하여, 200 k 에서 293 K 까지는 극성광학산란에 의하여 감소하는 경향이 나타냈다. 광전도 셀의 특성으로 스텍트럼 응답, 감도(γ), 최대허용소비전력 및 응답 시간을 측정하였다. Polycrystalline CdSe thin films were grown on creamic substrate using a chemical bath deposition (CBD) method. They were annealed at various temperature and X-ray diffraction patterns were measured by X-ray diffractometer in order to study CdSe polycrystal structure. Using extrapolation method of X-ray diffraction patterns for the CdSe samples annealed in N_2 gas at 450℃ it was found hexagonal structure whose lattice parameters a_o and c_o were 4.302 Å and 7.014 Å, respectively. It grain size was about 0.3 ㎛. Hall effect on this sample was measured by Van der Pauw method and studied on carrier density and mobility depending on temperature. From Hall data, the mobility was likely to be decreased by piezo electric scattering at temperature range of 33 K and 200 K, and by polar optical potical scattering at temperature range of 200 K and 293 K. We measured also spectral response, sensitivily (γ), maximum allowable power dissipation and response time on these samples.

^(32)P-postlabelling法을 이용한 有機溶劑 작업장 근로자의 遺傳毒性 評價

홍대용,김장락,이장호,문중갑,이한우,김동일,박성학,정주화,이홍근 한국환경독성학회 1994 환경독성보건학회지 Vol.9 No.1

To evaluate the genotoxicities of workers exposed to glue and glue cleaning solution, ambient air monitoring of working place, animal study and human monitoring were carried out. By GC-MS analysis, air samples collected from shoesmaking plant were found to be toluene, xylene, cyclohexane, n-hexane, methyl ethyl ketone, trichloroethylene, butylacetate, isopropyl alcohol. Glue and glue cleaning solution from shoesmaking plant were applicated topically to the CD-1 mice. DNA was isolated from skin 24 hr following the application and analysed for DNA-adducts using the nuclease P₁ version of ^(32)P-postlabelling assay. RAL (Relative Adduct Labelling, adducts/10^(8) nucleotides) was significantly increased in a dose-dependent manner in the glue cleaning solution treated mice skin. Peripheral blood DNA-adducts of workers exposed to glue and glue cleaning solution were also analysed by the same method, but there were not significant differences in the peripheral blood DNA-adducts level between exposed and control workers. In addition, glue cleaning solution from shoes factory was evaluated for mutagenicity in the Salmonella plate incorporation assay using strains TA 100 and TA 1535 in the presence and absence of Arochlor 1254-induced rat liver S_(9). There was evident mutagenicity for cleaning solution in TA 100 regardless of S_(9), but TA 1535 showed positive only in the absence of S_(9) when predicted by Stead model of mutagenicity prediction (p=0.0000). The urine concentrates from workers and controls were also assayed for mutagenicity towards strain TA 100 of Salmonella typhimurium in the presence of S_(9) using Kado's microsuspension assay, but their mutagenic activities were not found to be significant. These data suggest that shoesmaking workers are exposed to genotoxic compounds and need to be monitored by testing the mutagenicity of human urines. However, ^(32)P postlabelling application requires further validation for the routine monitoring of human exposure.