간호대학생의 단기 해외어학연수를 통한 자기인식 경험

손미라 ( Son¸ Mira ),김신향 ( Kim¸ Sinhyang ) 한국간호과학회 정신간호학회(구 대한간호학회정신간호학회) 2021 정신간호학회지 Vol.30 No.1

Purpose: The purpose of this study was to explore the experience of self-awareness of nursing students through a short-term overseas language study. Methods: Study subjects were 10 nursing students, and collected data were analyzed with open coding, axial coding, selective coding, and process analysis based on Strauss and Corbin's grounded theory (1998). Results: In total, 23 sub-categories and 12 categories were generated. The 12 categories included "Longing for a bigger world", "Don't want to be intimidated", "Interest in English", "Tension about local classes", "Feeling of pushing the limit", "Getting along with natives", “Making Korean American University students a role model", "Self-observe confronting unfamiliarity", "Self-encourage about enduring hardships", "Self-expansion for a better self", "Recognize self-transformation", "Focusing on the reality". Nursing students' self-awareness experience through a short-term overseas language study was a process of solving the phenomenon of 'feeling of pushing the limit', and a derived core category was 'surpass the feeling of pushing the limit and take a step forward'. Conclusion: Study participants experienced self-awareness. These results will not only expand our understanding about self-awareness of nursing students and educators but will also provide information for developing a short-term overseas study program.

The KAAACI/KDA Evidence-Based Practice Guidelines for Chronic Spontaneous Urticaria in Korean Adults and Children: Part 1. Definition, Methodology and First-line Management

송우정,Mira Choi,Dong Hun Lee,Jae-Woo Kwon,Gun-Woo Kim,Myung Hwa Kim,Mi-Ae Kim,Min-Hye Kim,Byung-Keun Kim,Sujeong Kim,Joung Soo Kim,Jung Eun Kim,Ju Young Kim,Joo Hee Kim,Hyun Jung Kim,Hye One Kim,Hyo-Bin 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.4

Chronic spontaneous urticaria (CSU) is defined as the occurrence of spontaneous wheals, angioedema, or both for >6 weeks in the absence of specific causes. It is a common condition associated with substantial disease burden both for affected individuals and societies in many countries, including Korea. CSU frequently persists for several years and requires high-intensity treatment; therefore, patients experience deteriorations in quality of life and medication-associated complications. During the last decade, there have been major advances in the pharmacological treatment of CSU and there is an outstanding need for evidence-based guidelines that reflect clinical practice in Korea. The guidelines reported here represent a joint initiative of the Korean Academy of Asthma, Allergy and Clinical Immunology and the Korean Dermatological Association, and aim to provide evidence-based guidance for the management of CSU in Korean adults and children. In Part 1, disease definition, guideline scope and development methodology as well as evidence-based recommendations on the use of antihistamines and corticosteroids are summarized.

Flow and surface pressure field measurements on a circular cylinder with impingement of turbulent round jet

Kim, Mirae,Li, Yongzeng,Peng, Di,Yeom, Eunseop,Kim, Kyung Chun Elsevier 2019 Experimental thermal and fluid science Vol.105 No.-

<P><B>Abstract</B></P> <P>When the circular jet impinges onto a cylinder, the three-dimensional (3D) curved wall jet was attached to the wall due to the Coanda effect and the flow separation was delayed with increasing Reynolds number and impinging angle. These interesting phenomena can be studied in depth through a variety of parameters and measurement methods. Time-resolved stereo PIV measurement was conducted to investigate the 3D flow characteristics with instantaneous velocity and vorticity fields. Pressure sensitive paint (PSP) method was used to examine the pressure distribution on the cylinder surface when a circular jet impinges on it. The effects of the impingement angle, Reynolds number, and diameter ratio between the jet nozzle and the cylinder (d/D) were examined at a fixed nozzle to wall distance ratio of two (H/d = 2). Experimental results show that the pressure gradient in the flow direction decreases and the separation is delayed when the Reynolds number and impingement angle are increased at the same distance ratio. As the diameter ratio decreases with the same impingement angle and Reynolds number, the maximum suction pressure increases, and the pressure gradient in the wall jet flow direction increases. The three-dimensional nature of the surface pressure distribution changes with the flow parameters, which are well explained with three-dimensional flow field measurements in the curved 3D wall jet due to the Coanda effect.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Surface pressure distribution was measured when a circular jet impinges on a cylinder. </LI> <LI> Effects of the impingement angle, Reynolds number, and diameter ratio were examined. </LI> <LI> Pressure gradient in flow direction decreases when the impingement angle increases. </LI> <LI> The three-dimensional nature of the surface pressure changes with the flow parameters. </LI> </UL> </P>

Derivation of putative brain endothelial cells from porcine induced pluripotent stem cells

Mirae Kim,Sang-Hwan Hyun 한국수정란이식학회 2017 한국수정란이식학회 학술대회 Vol.2017 No.05

The use of pigs in neuroscience has increased over the past years because the pigs are closely related to humans in terms of anatomy and physiology. Especially, the blood-brain barrier (BBB) maintains the homeostatic microenvironment in the central nervous system (CNS) and they can provide a valuable tool for studying the neurobiology. However, only a few putative blood-brain barrier (BBB) models have been generated by co-culture of porcine primary cells. The fundamental problem is that they lose some of their phenotypes when maintained in vitro for long-term culture. To establish improved in vitro porcine BBB models, we differentiated novel brain microvascular endothelial cells (BMECs) from porcine induced pluripotent stem cells (iPSCs) using a modified human-based protocol. Briefly, the dissociated single cells from iPSCs were seeded in Geltrex. For differentiation, cells were maintained for 3 days of expansion and then switched to unconditioned medium (UM) lacking bFGF for 6-7 days. Then, we subcultured cells onto collagen/fibronectin coated plates and changed BMEC medium for 2-3 weeks. About two weeks later, we observed a cluster of round cells surrounded by spindle shaped adherent cells termed as colony-forming units (CFU) of putative BMECs. Over time, the cluster of cells disappears and remained adherent spindle-shaped cells showed properties of endothelial cells. Although further studies will be needed, this study would be a great comparative analysis of the porcine and human in vitro BBB model.

PSME4 determines mesenchymal stem cell fate towards cardiac commitment through YAP1 degradation

Mira Kim,Yong Sook Kim,Youngkeun Ahn,Gwang Hyeon Eom,Somy Yoon The Korean Society of Pharmacology 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.4

The regeneration of myocardium following acute circulatory events remains a challenge, despite numerous efforts. Mesenchymal stem cells (MSCs) present a promising cell therapy option, but their differentiation into cardiomyocytes is a time-consuming process. Although it has been demonstrated that PSME4 degrades acetyl-YAP1, the role of PSME4 in the cardiac commitment of MSCs has not been fully elucidated. Here we reported the novel role of PSME4 in MSCs cardiac commitment. It was found that overnight treatment with apicidin in primary-cultured mouse MSCs led to rapid cardiac commitment, while MSCs from PSME4 knock-out mice did not undergo this process. Cardiac commitment was also observed using lentivirus-mediated PSME4 knockdown in immortalized human MSCs. Immunofluorescence and Western blot experiments revealed that YAP1 persisted in the nucleus of PSME4 knockdown cells even after apicidin treatment. To investigate the importance of YAP1 removal, MSCs were treated with shYAP1 and apicidin simultaneously. This combined treatment resulted in rapid YAP1 elimination and accelerated cardiac commitment. However, overexpression of acetylation-resistant YAP1 in apicidin-treated MSCs impeded cardiac commitment. In addition to apicidin, the universal effect of histone deacetylase (HDAC) inhibition on cardiac commitment was confirmed using tubastatin A and HDAC6 siRNA. Collectively, this study demonstrates that PSME4 is crucial for promoting the cardiac commitment of MSCs. HDAC inhibition acetylates YAP1 and facilitates its translocation to the nucleus, where it is removed by PSME4, promoting cardiac commitment. The failure of YAP1 to translocate or be eliminated from the nucleus results in the MSCs' inability to undergo cardiac commitment.

Oral Supplementation with Cocoa Extract Reduces UVB-Induced Wrinkles in Hairless Mouse Skin

Kim, Jong-Eun,Song, Dasom,Kim, Junil,Choi, Jina,Kim, Jong Rhan,Yoon, Hyun-Sun,Bae, Jung-Soo,Han, Mira,Lee, Sein,Hong, Ji Sun,Song, Dayoung,Kim, Seong-Jin,Son, Myoung-Jin,Choi, Sang-Woon,Chung, Jin Ho Elsevier 2016 The Journal of investigative dermatology Vol.136 No.5

<P>Cacao beans contain various bioactive phytochemicals that could modify the pathogeneses of certain diseases. Here, we report that oral administration of cacao powder (CP) attenuates UVB-induced skin wrinkling by the regulation of genes involved in dermal matrix production and maintenance. Transcriptome analysis revealed that 788 genes are down-or upregulated in the CP supplemented group, compared with the UVB-irradiated mouse skin controls. Among the differentially expressed genes, cathepsin G and serpin B6c play important roles in UVB-induced skin wrinkle formation. Gene regulatory network analysis also identified several candidate regulators responsible for the protective effects of CP supplementation against UVB-induced skin damage. CP also elicited antiwrinkle effects via inhibition of UVB-induced matrix metalloproteinases-1 expression in both the human skin equivalent model and human dermal fibroblasts. Inhibition of UVB-induced activator protein-1 via CP supplementation is likely to affect the expression of matrix metalloproteinases-1. CP supplementation also downregulates the expression of cathepsin G in human dermal fibroblasts. 5-(3',4'-Dihydroxyphenyl)-gamma-valerolactone, a major in vivo metabolite of CP, showed effects similar to CP supplementation. These results suggest that cacao extract may offer a protective effect against photoaging by inhibiting the breakdown of dermal matrix, which leads to an overall reduction in wrinkle formation.</P>

Electrically Conducting and Mechanically Strong Graphene-Polylactic Acid Composites for 3D Printing

Kim, Mirae,Jeong, Jae Hwan,Lee, Jong-Young,Capasso, Andrea,Bonaccorso, Francesco,Kang, Seok-Hyeon,Lee, Young-Kook,Lee, Gwan-Hyoung American Chemical Society 2019 ACS APPLIED MATERIALS & INTERFACES Vol.11 No.12

<P>The advent of 3D printing has had a disruptive impact in manufacturing and can potentially revolutionize industrial fields. Thermoplastic materials printable into complex structures are widely employed for 3D printing. Polylactic acid (PLA) is among the most promising polymers used for 3D printing, owing to its low cost, biodegradability, and nontoxicity. However, PLA is electrically insulating and mechanically weak; this limits its use in a variety of 3D printing applications. This study demonstrates a straightforward and environment-friendly method to fabricate conductive and mechanically reinforced PLA composites by incorporating graphene nanoplatelets (GNPs). To fully utilize the superior electrical and mechanical properties of graphene, liquid-exfoliated GNPs are dispersed in isopropyl alcohol without the addition of any surfactant and combined with PLA dissolved in chloroform. The GNP-PLA composites exhibit improved mechanical properties (improvement in tensile strength by 44% and maximum strain by 57%) even at a low GNP threshold concentration of 2 wt %. The GNP-PLA composites also exhibit an electrical conductivity of over 1 mS/cm at >1.2 wt %. The GNP-PLA composites can be 3D-printed into various features with electrical conductivity and mechanical flexibility. This work presents a new direction toward advanced 3D printing technology by providing higher flexibility in designing multifunctional 3D printed features.</P> [FIG OMISSION]</BR>

Effect of frost on phosphorescence for thermographic phosphor thermometry

Kim, Dong,Kim, Mirae,Kim, Kyung Chun IOP Pub 2017 Measurement science & technology Vol.28 No.12

<P>In this study, we analyzed phosphorescence lifetime and its accuracy by growing frost for thermographic phosphor thermometry in a low-temperature environment. Mg<SUB>4</SUB>FGeO<SUB>6</SUB>:Mn particles were coated on an aluminum plate and excited with a UV-LED to obtain phosphorescence signals. The surface temperature was maintained at  −20, −15, −10 °C, and the phosphorescence signal was acquired as the frost grew for 3700 s. The lifetime was calculated and compared with the calibration curve under no-frost conditions. The error of the measured lifetime was within 0.7% of that in the no-frost conditions. A 2D surface temperature profile of the target plate was successfully obtained with the frost formation.</P>

Flow Characteristics of Impinging Jet onto a Cylinder

Mirae Kim(김미래),Hyun Dong Kim(김현동),Kyung Chun Kim(김경천) 한국가시화정보학회 2016 한국가시화정보학회 학술발표대회 논문집 Vol.2016 No.12

A New Method for Aortic Valve Planimetry with High-Resolution 3-Dimensional MRI and Its Comparison with Conventional Cine MRI and Echocardiography for Assessing the Severity of Aortic Valvular Stenosis

Kim Hae Jin,Choe Yeon Hyeon,Kim Sung Mok,Kim Eun Kyung,Lee Mirae,Park Sung-Ji,Ahn Joonghyun,Carriere Keumhee C. 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.8

Objective: We aimed to compare the aortic valve area (AVA) calculated using fast high-resolution three-dimensional (3D) magnetic resonance (MR) image acquisition with that of the conventional two-dimensional (2D) cine MR technique. Materials and Methods: We included 139 consecutive patients (mean age ± standard deviation [SD], 68.5 ± 9.4 years) with aortic valvular stenosis (AS) and 21 asymptomatic controls (52.3 ± 14.2 years). High-resolution T2-prepared 3D steady-state free precession (SSFP) images (2.0 mm slice thickness, 10 contiguous slices) for 3D planimetry (3DP) were acquired with a single breath hold during mid-systole. 2D SSFP cine MR images (6.0 mm slice thickness) for 2D planimetry (2DP) were also obtained at three aortic valve levels. The calculations for the effective AVA based on the MR images were compared with the transthoracic echocardiographic (TTE) measurements using the continuity equation. Results: The mean AVA ± SD derived by 3DP, 2DP, and TTE in the AS group were 0.81 ± 0.26 cm2, 0.82 ± 0.34 cm2, and 0.80 ± 0.26 cm2, respectively (p = 0.366). The intra-observer agreement was higher for 3DP than 2DP in one observer: intraclass correlation coefficient (ICC) of 0.95 (95% confidence interval [CI], 0.94–0.97) and 0.87 (95% CI, 0.82–0.91), respectively, for observer 1 and 0.97 (95% CI, 0.96–0.98) and 0.98 (95% CI, 0.97–0.99), respectively, for observer 2. Inter-observer agreement was similar between 3DP and 2DP, with the ICC of 0.92 (95% CI, 0.89–0.94) and 0.91 (95% CI, 0.88–0.93), respectively. 3DP-derived AVA showed a slightly higher agreement with AVA measured by TTE than the 2DP-derived AVA, with the ICC of 0.87 (95% CI, 0.82–0.91) vs. 0.85 (95% CI, 0.79–0.89). Conclusion: High-resolution 3D MR image acquisition, with single-breath-hold SSFP sequences, gave AVA measurement with low observer variability that correlated highly with those obtained by TTE.