Characterization of Ecdysteroid UDP-Glucosyltransferase Gene Promoter from Bombyx mori Nucleopolyhedrovirus

( Xing Jia Shen ),( Yong Zhu Yi ),( Shun Ming Tang ),( Zhi Fang Zhang ),( Yi Ren Li ),( Jia Lu He ),( Xiang Fu Wu ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.2

Bombyx mori nucleopolyhedrovirus(BmNPV) ecdysteroid UDP-glucosyltransferase gene (egt) promoter fragments of different lengths were amplified from BmNPV ZJ-8 genomic DNA by PCR. Reporter plasmids pBmegt542-luc, pBmegt309-luc and pBmegtl59-luc with luciferase (luc) driven by egt promoters were constructed. Both in vitro and in vivo expressions showed that BmNPV egt promoter activity requires the transactivation of viral factor(s), and expression of luc was detected earliest at 24 hrs post infection (pi). BmNPV ZJ-8 homologous region 3 (hr3) increased the expression of luc by over 1,600-fold. Molting hormone of 1.0∼2.0㎍/㎖ can dramatically down regulate expression of luc. Juvenile hormone analogue of 0.5∼2.0㎍/㎖ increased expression of luc by 145.8% to 75.7%. Deletion assay revealed that the promoter fragment of 159 bp contains the basal promoter structure; Promoter fragments of 309 bp and 542 bp showed similar but much higher transcriptional activities than that of 159 bp, suggesting that nucleotide from -159 to -309 nt upstream the translation initiation site harbors the main cis-acting elements.

Characterization of Ecdysteroid UDP-Glucosyltransferase Gene Promoter from Bombyx mori Nucleopolyhedrovirus

Xing-Jia Shen,Yong-Zhu Yi,Shun-Ming Tang,Zhi-Fang Zhang,Yi-Ren Li,Jia-Lu He,Xiang-Fu Wu 한국잠사학회 2004 International Journal of Industrial Entomology Vol.8 No.2

Bombyx mori nucleopolyhedrovirus (BmNPV) ecdysteroid UDP-glucosyltransferase gene (egt) promoter fragments of different lengths were amplified from BmNPV ZJ-8 genomic DNA by PCR. Reporter plasmids pBmegt542-luc, pBmegt309-luc and pBmegt159- luc with luciferase (luc) driven by egt promoters were constructed. Both in vitro and in vivo expressions showed that BmNPV egt promoter activity requires the transactivation of viral factor(s), and expression of luc was detected earliest at 24 hrs post infection (pi). BmNPV ZJ-8 homologous region 3 (hr3) increased the expression of luc by over 1,600-fold. Molting hormone of 1.0 ~ 2.0 mg/ml can dramatically down regulate expression of luc. Juvenile hormone analogue of 0.5 ~ 2.0 mg/ml increased expression of luc by 145.8% to 75.7%. Deletion assay revealed that the promoter fragment of 159 bp contains the basal promoter structure; Promoter fragments of 309 bp and 542 bp showed similar but much higher transcriptional activities than that of 159 bp, suggesting that nucleotide from -159 to -309 nt upstream the translation initiation site harbors the main cis-acting elements.

LncRNA TMPO-AS1 promotes esophageal squamous cell carcinoma progression by forming biomolecular condensates with FUS and p300 to regulate TMPO transcription

Luo Xiao-Jing,He Ming-Ming,Liu Jia,Zheng Jia-Bo,Wu Qi-Nian,Chen Yan-Xing,Meng Qi,Luo Kong-Jia,Chen Dong-Liang,Xu Rui-Hua,Zeng Zhao-Lei,Liu Ze-Xian,Luo Hui-Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Esophageal squamous cell carcinoma (ESCC) is one of the most life- and health-threatening malignant diseases worldwide, especially in China. Long noncoding RNAs (lncRNAs) have emerged as important regulators of tumorigenesis and tumor progression. However, the roles and mechanisms of lncRNAs in ESCC require further exploration. Here, in combination with a small interfering RNA (siRNA) library targeting specific lncRNAs, we performed MTS and Transwell assays to screen functional lncRNAs that were overexpressed in ESCC. TMPO-AS1 expression was significantly upregulated in ESCC tumor samples, with higher TMPOAS1 expression positively correlated with shorter overall survival times. In vitro and in vivo functional experiments revealed that TMPO-AS1 promotes the proliferation and metastasis of ESCC cells. Mechanistically, TMPO-AS1 bound to fused in sarcoma (FUS) and recruited p300 to the TMPO promoter, forming biomolecular condensates in situ to activate TMPO transcription in cis by increasing the acetylation of histone H3 lysine 27 (H3K27ac). Targeting TMPO-AS1 led to impaired ESCC tumor growth in a patient-derived xenograft (PDX) model. We found that TMPO-AS1 is required for cell proliferation and metastasis in ESCC by promoting the expression of TMPO, and both TMPO-AS1 and TMPO might be potential biomarkers and therapeutic targets in ESCC.

MiR-221 promotes trastuzumab-resistance and metastasis in HER2-positive breast cancers by targeting PTEN?

( Xing Ming Ye ),( Wen Dong Bai ),( Hua Yu Zhu ),( Xiao Zhang ),( Ying Chen ),( Lei Wang ),( An Gang Yang ),( Jing Zh Ao ),( Lin Tao Jia ) 생화학분자생물학회 2014 BMB Reports Vol.47 No.5

HER2-overexpressing breast cancers are characterized byfrequent distant metastasis and often develop resistance aftershort-term effective treatment with the monoclonal antibodydrug, trastuzumab. Here, we found that the oncogenic miRNA,miR-221, inhibited apoptosis, induced trastuzumab resistanceand promoted metastasis of HER2-positive breast cancers. Thetumor suppressor PTEN was identified as a miR-221 target;overexpression of PTEN abrogated the aforementionedmiR-221-induced malignant phenotypes of the cells. Thesefindings indicate that miR-221 may promote trastuzumabresistance and metastasis of HER2-positive breast cancers bytargeting PTEN, suggesting its role as a potential biomarker forprogression and poor prognosis, and as a novel target fortrastuzumab-combined treatment of breast cancers.[BMB Reports 2014; 47(5): 268-273]

Molecule-based electrorheological material with luminescence property

Ming-Xing Chen,Fu-Hui Liao,Yan-Li Shang,Yun-Ling Jia,Jun-Ran Li 한국유변학회 2013 Korea-Australia rheology journal Vol.25 No.1

Molecule-based electrorheological (ER) materials with luminescence property, based on β-cyclodextrin [(C6O5H10)7, β-CD] inclusion compounds between β-CD (host) and the rare earth (RE) (RE=Tb, Eu) complex (guest), have been synthesized as a novel type of ER materials using β-CD, Tb(NO3)3, Eu(NO3)3, sulphosalicylic acid (C7H6O6S·2H2O, SSA) and m-phthalic acid (C8H6O4, MPA) as original materials. The composition, ER performance, luminescence property and dielectric property of the materials have been studied. The results show that the rare earth complex in the cavity of β-CD may enhance the ER performance of β-CD, and the complex (Tb-SSA) of Tb3+ can improve more effectively the ER activity of β-CD than that (Eu-MPA) of Eu3+ among both of the complexes. The composition and structure are the dominant factors in improving the ER effect. The fluorescence intensity, fluorescence lifetime and emission quantum yield of the particle materials and their suspensions in silicone oil have been tested, and fine luminescence performance has been detected. The material with ER activity and luminescence performance is a novel multifunctional material which would have wide application prospect.

Research on Flame Retardant Formaldehyde-Free Plywood Glued by Aqueous Polymer Isocyanate Adhesive

( Ming-yu Wen ),( Jia-zhi Zhu ),( Meng Zhu ),( Yao-xing Sun ),( Hee-jun Park ),( Junyou Shi ) 한국목재공학회 2020 목재공학 Vol.48 No.5

Due to pronounced mechanical performance and being environmental friendly, aqueous polymer isocyanate adhesive (API) has been widely applied in the production of formaldehyde-free wood products. In this study, flame retardant formaldehyde-free plywood was prepared by incorporation of flame retardants into the API adhesive. Partially phosphorylated poly (vinyl alcohol) (PPVA) which was prepared by reacting poly (vinyl alcohol) with phosphoric acid was used to replace PVA in API formula. In addition, Mg-Al layered double hydroxides (LDH) was chosen as additive flame retardant, replacing traditional filler CaCO₃ in API adhesive formula. And then, the flame retardant API adhesive with main agent (PPVA replacing PVA70wt.%, SBR emulsion 30wt.%), curing agent 10wt.% (accounts for of the main agent), and 20wt.% LDHs (accounts of the main agent) was used to prepare flame retardant plywood. The effect of application of PPVA and Mg-Al LDH on bonding strength of plywood was investigated. The flammability characteristics of the plywood were determined by cone calorimeter test (CCT). The results revealed that compared with the plywood prepared with API adhesive, the use of PPVA and LDH enhanced the flame retardancy of plywood without negatively affecting bonding strength. The CCT tests indicated that the heat release and smoke production flame retardant API plywood were lower than those of the ordinary API glued plywood. Promising developments for flame retardant API adhesive were expected in future applications of flame retardant formaldehyde-free plywood.

A reliability analysis method for rock slope controlled by weak structural surface

Jia-wen Zhou,Ming-yuan Jiao,Hui-ge Xing,Xing-guo Yang,Yu-chuan Yang 한국지질과학협의회 2017 Geosciences Journal Vol.21 No.3

Catastrophic landslides maybe occur in rock slope due to the effect of strong earthquakes or heavy rainfall. The stability of rock slope is usually controlled by different scales of weak structural surfaces, which are uncertain and randomly exist in the rock slope. According to the geological characteristics of rock slope, two typical failure modes – plane and wedge are possible. A second-order second-moment (SOSM) method is presented to calculate the reliability index and the failure probability of rock slope, which is an improvement over the first-order second-moment (FOSM) method, and performance functions are built up with the classic limit equilibrium method. The presented method is applied to analyze the failure probability of two rock slopes at the Jinping I Hydropower Station and is compared with the Monte Carlo method and the FOSM method. The computed results show that for plane failure, the reliability index and the failure probability determined by the presented method are 0.563 and 28.7%, respectively, and the reliability index and the failure probability determined by Monte Carlo method are 0.677 and 24.9%, respectively. However, for the FOSM method, the reliability index and failure probability are –0.025 and 51.0%, respectively. For both plane failure and wedge failure, the difference between the presented method and the Monte Carlo method is very small, but the failure probability of plane failure determined by FOSM method is larger than that of the other two methods. The presented method can provide a useful tool to evaluate the failure probability of rock slope.

New multifunction materials with both electrorheological performance and luminescence property

Ming-Xing Chen,Yan-Li Shang,Yun-Ling Jia,Xiang-Yu Dong,Jing Ren,Jun-Ran Li 한국유변학회 2017 Korea-Australia rheology journal Vol.29 No.1

Novel multifunctional materials, the composites AlOOH-NaYFTb5 and AlOOH-NaYFTb10, containing AlO(OH) and β-NaYF4:5%Tb3+, have been synthesized via a facile hydrothermal route and a simple grinding method. The boehmite [AlO(OH)], yttrium nitrate [Y(NO3)3·6H2O], terbium nitrate, [Tb(NO3)3·6H2O], sodium citrate (Na3C6H5O7·2H2O) and sodium fluoride (NaF) were used as starting materials. The composition, electrorheological (ER) performance, and luminescence property of the functional materials were studied. Our results show that the composites not only have good electrorheological (ER) performance, but also have good optics property. The relative shear stress τr (τr = τE/τ0, τE and τ0 are the shear stresses at the electric field strength E = 4 and 0 kV/mm, respectively) values of the suspension (25 wt.%) of AlOOHNaYFTb5 material in silicone oil are all larger than 50 in a shear rate ranging from 0.06 to 26 s−1, the τr value reaches 1333 at a shear rate of 0.06 s−1. The material with such high ER activity and favorable luminescence performance is advantageous in its application as a multifunctional material.

Betulin Targets Lipin1/2-Meidated P2X7 Receptor as a Therapeutic Approach to Attenuate Lipid Accumulation and Metaflammation

( Jia-yi Dou ),( Yu-chen Jiang ),( Zhong-he Hu ),( Kun-chen Yao ),( Ming-hui Yuan ),( Xiao-xue Bao ),( Mei-jie Zhou ),( Yue Liu ),( Zhao-xu Li ),( Li-hua Lian ),( Ji-xing Nan ),( Yan-ling Wu ) 한국응용약물학회 2022 Biomolecules & Therapeutics(구 응용약물학회지) Vol.30 No.3

The present study focused on the potential mechanism of betulin (BT), a pentacyclic triterpenoid isolated from the bark of white birch (Betula pubescens), against chronic alcohol-induced lipid accumulation and metaflammation. AML-12 and RAW 264.7 cells were administered ethanol (EtOH), lipopolysaccharide (LPS) or BT. Male C57BL/6 mice were fed Lieber-DeCarli liquid diets containing 5% EtOH for 4 weeks, followed by single EtOH gavage on the last day and simultaneous treatment with BT (20 or 50 mg/kg) by oral gavage once per day. In vitro, MTT showed that 0-25 mM EtOH and 0-25 μM BT had no toxic effect on AML-12 cells. BT could regulate sterolregulatory-element-binding protein 1 (SREBP1), lipin1/2, P2X7 receptor (P2X7r) and NOD-like receptor family, pyrin domains-containing protein 3 (NLRP3) expressions again EtOH-stimulation. Oil Red O staining also indicated that BT significantly reduced lipid accumulation in EtOH-stimulated AML-12 cells. Lipin1/2 deficiency indicated that BT might mediate lipin1/2 to regulate SREBP1 and P2X7r expression and further alleviate lipid accumulation and inflammation. In vivo, BT significantly alleviated histopathological changes, reduced serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and triglyceride (TG) levels, and regulated lipin1/2, SREBP1, peroxisome proliferator activated receptor α/γ (PPARα/γ) and PGC-1α expression compared with the EtOH group. BT reduced the secretion of inflammatory factors and blocked the P2X7r-NLRP3 signaling pathway. Collectively, BT attenuated lipid accumulation and metaflammation by regulating the lipin1/2-mediated P2X7r signaling pathway.

Biotechnology : Microbial Desulfurization of Dibenzothiophene and 4,6-Dimethyldibenzothiophene in Dodecane and Straight-Run Diesel Oil

( Luo Ming Fang ),( Xing Jian Min ),( Gou Zhong Xuan ),( Liu Hui Zhou ),( Chen Jia Yong ) 한국화학공학회 2003 Korean Journal of Chemical Engineering Vol.20 No.4

The desulfurization of dibenzothiophene (DBT), 4,6-dimethyldibenzothiophene (4,6-DMDBT) and their mixture by lyophilized cells of Pseudomonas delafieldii R-8 was studied in the presence of dodecane. The desulfurization rate for 4,6-DMDBT was found to be about 40% in comparison with that for DBT. The desulfurization process for DBT and 4,6-DMDBT proceeded simultaneously without preference for either one. The desulfurization rate for each compound was decreased when they were mixed together. The extent of desulfurization of 4,6-DMDBT was increased with the increase of cell concentration and the decrease of the volume ratio of oil-to-water used. The specific desulfurization rate for 4,6-DMDBT could be reached to 10.4 mmol sulfur kg^(-1) (cell) h^(-1) [approximately 0.33 mg sulfur g^(-1) (cell) h^(-1)]. Pseudomonas delafieldii R-8 showed high desulfurization capability for straight-run diesel oil (containing 1,807 mg/L of sulfur). About 1,000 mg/L of sulfur in diesel oil was removed by resting cells of this strai in 24 h of reaction. The specific desulfurization rate was 8.75 mmol sulfur kg^(-1) (cell) h^(-1).