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Amperometric Determination of Nitrite at Poly(Methylene Blue)-Modified Glassy Carbon Electrode
Xu, Guang-Ri,Xu, Guifang,Xu, Ming-Lu,Zhang, Zhengqing,Tian, Yuan,Choi, Han-Nim,Lee, Won-Yong Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.2
Electrochemical characteristics of nitrite ion were investigated at a poly(methylene blue)-modified glassy carbon electrode by cyclic voltammetry and differential pulse voltammetry. The poly(methylene blue)-modified glassy carbon electrode exhibited enhanced anodic signals for nitrite. The effects of key parameters on the detection of nitrite were evaluated at the modified electrode, such as pH, accumulation time, and scan rate. Under optimum condition, the chemically modified electrode can detect nitrite in the concentration range $2.0{\times}10^{-6}$ to $5.0{\times}10^{-4}$ M with the detection limit of $2.0{\times}10^{-6}$ M and a correlation coefficient of 0.999. The detection of nitrite using the chemically modified electrode was not affected by common ions such as $Na^+$, $K^+$, $Ca^{2+}$, $Cl^-$, $HPO_4^{2-}$ and $H_2PO_4^- $. The modified electrode showed good stability and reproducibility. The practical application of the present method was successfully applied to the determination of nitrite ion in cabbage samples.
Real-time online optimal control of current-fed dual active bridges based on machine learning
Han, Ming,Liu, Xiaosheng,Pu, Honghong,Zhao, Liang,Wang, Kaixuan,Xu, Dianguo The Korean Institute of Power Electronics 2020 JOURNAL OF POWER ELECTRONICS Vol.20 No.1
This paper proposes a real-time online optimal (RT-OPT) control method based on machine learning for a current-fed dual active bridge (CF-DAB). The basis of this control strategy is the linear quadratic optimal control, which designs the sliding surface and realizes power control based on sliding mode control (SMC). For the parameters of Q and R in the objective function of the linear quadratic regulator (LQR), a genetic algorithm is used to find the optimal value, and the optimal value is taken as the sample data. Through machine learning offline training, a neural network is obtained and run online to realize real-time online optimal control. The control method was verified by simulations in MATLAB/Simulink. The RT-OPT method achieves the expected functionality, and has better dynamic and steady-state performance than the PI controller.
Prognostic Significance of Beclin-1 Expression in Colorectal Cancer: a Meta-analysis
Han, Ye,Xue, Xiao-Feng,Shen, Hu-Gang,Guo, Xiao-Bo,Wang, Xu,Yuan, Bin,Guo, Xing-Po,Kuang, Yu-Ting,Zhi, Qiao-Ming,Zhao, Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11
Objective: Beclin-1 has recently been observed as an essential marker of autophagy in several cancers. However, the prognostic role of Beclin-1 in colorectal neoplasia remains controversial. Our study aimed to evaluate the potential association between Beclin-1 expression and the outcome of colorectal cancer patients. Materials and Methods: All related studies were systematically searched in Pubmed, Embase, Springer and Chinese National Knowledge Infrastructure databases (CNKI), and then a meta-analysis was performed to determine the association of Beclin-1 expression with clinical outcomes. Finally, a total of 6 articles were included in our analysis. Results: Our data showed that high Beclin-1 expression in patients with CRC was associated with poor prognosis in terms of tumor distant metastasis (OR=2.090, 95%CI=1.061-4.119, p=0.033) and overall survival (RR=1.422, 95%CI=1.032-1.959, p=0.031). However, we did not found any correlation between Beclin-1 over-expression and tumor differentiation (OR=1.711, 95%CI=0.920-3.183, p=0.090). In addition, there was no evidence of publication bias as suggested by Egger's tests for tumor distant metastasis (p=1.000), differentiation (p=1.000) and OS (p=0.308). Conclusions: Our present meta-analysis indicated that elevated Beclin-1 expression iss associated with tumor metastasis and a poor prognosis in patients with CRC. Beclin-1 might serve as an efficient prognostic indicator in CRC, and could be a new molecular target in CRC therapy.
Stability Analysis of Closely Star-battened Member of Transmission Tower
Han Jun-ke,Zhang Chun-lei,Li Zhen-bao,Wang Xu-ming 한국강구조학회 2017 International Journal of Steel Structures Vol.17 No.3
Stability of the closely star-battened member of a power transmission tower were researched in this paper, a theoretical analysis was conducted for the buckling mode of the closely star-battened member by different plate batten arrangements. The analytical method of the buckling capacity on the closely star-battened member was proposed here considering an arrangement type of the plate batten and they are compared with the results of a full-scale test. The conclusions are follows: the flexuraltorsional buckling did not happened for the closely star-battened member; the flexural buckling need to be calculated only after the strength reduction factor was considered; the stability-bearing capacity of the closely star-battened member of the line-shape type batten is slightly less than that of the cross-shape type batten; the stability-bearing capacity of the closely star-battened member can be improved when the number of bolts in the plate batten increases in a suitable extent; and the stability-bearing capacity calculated and test results agree well with each other when the stability-bearing capacity analytical method of the closely star-battened member was used to analyze the full-scale test tower. The experimental results show the calculation method is reasonable.
Threshold magnetoresistance in anistropic magnetic 2D transition metal dichalcogenides
Xu, Hongjun,Hsu, Ming-Chien,Fuh, Huei-Ru,Feng, Jiafeng,Han, Xiufeng,Zhao, Yanfeng,Zhang, Duan,Wang, Xinming,Liu, Fang,Liu, Huajun,Cho, Jiung,Choi, Miri,Chun, Byong Sun,Ó,Coileá,in, Cormac The Royal Society of Chemistry 2018 Journal of Materials Chemistry C Vol.6 No.12
<P>Recently many novel magnetoresistance (MR) phenomena have been reported from studies of two dimensional (2D) materials. Here, we report on the exotic transport behavior of VS2. A large negative and quadratic MR of −10% is observed for an in-plane magnetic field B up to 14 T. Remarkably, when the applied field deviates from the in-plane orientation there is a threshold field, Bc, and the MR shows a plateau of near zero MR. When B < Bc, only a single state exists and the transition between quantum spin states is forbidden. Our work sheds new light on the MR of magnetic 2D materials with localized states and may spur further investigations.</P>
Han, Yan,Bo, Zhi-Jian,Xu, Ming-Yu,Sun, Nan,Liu, Dan-Hong The Korean Society of Pharmacology 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.3
In this study we aim to extensively investigate the anti-influenza virus immune responses in human pharyngeal epithelial cell line (Hep-2) and evaluate the protective role of Toll-like receptor (TLR) ligands in seasonal influenza A H1N1 (sH1N1) infections in vitro. We first investigated the expression of the TLRs and cytokines genes in resting and sH1N1 infected Hep-2 cells. Clear expressions of TLR3, TLR9, interleukin (IL)-6, tumour necrosis factor (TNF)-${\alpha}$ and interferon (IFN)-${\beta}$ were detected in resting Hep-2 cells. After sH1N1 infection, a ten-fold of TLR3 and TLR9 were elicited. Concomitant with the TLRs activation, transcriptional expression of IL-6, TNF-${\alpha}$ and IFN-${\beta}$ were significantly induced in sH1N1-infected cells. Pre-treatment of cells with poly I:C (an analog of viral double-stranded RNA) and CpG-ODN (a CpG-motif containing oligodeoxydinucleotide) resulted in a strong reduction of viral and cytokines mRNA expression. The results presented indicated the innate immune response activation in Hep-2 cells and affirm the antiviral role of Poly I:C and CpG-ODN in the protection against seasonal influenza A viruses.
Human-Oriented Recognition for Intelligent Interactive Office Robot
Chia-Ming Wang,Shin-Huan Tseng,Pei-Wen Wu,Yuan-Han Xu,Chien-Ke Liao,Yu-Chi Lin,Yi-Shiu Chiang,Chung-Dial Lim,Ting-Sheng Chu,Li-Chen Fu 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10
This paper presents our new intelligent interactive robot, which is constructed to eagerly provide multi-functional services in an office environment. In order to endow a full interactive capability of our robots for realizing so-called human-robot interaction (HRI), we propose sensor fusion based human detection and tracking system and human pose estimation to deal with a number of situations which may take place in the office environment. Not only by these perceptions, human interact with the robot also by some natural way, such as touching the interface screen and talking with the robot through microphone. Finally, the effectiveness of the proposed work is tested and validated by some of experiments.
( Bo Xu ),( Li Ming Dai ),( Jun Jun Li ),( Meng Deng ),( Hua Biao Miao ),( Jun Pei Zhou ),( Yue Lin Mu ),( Qian Wu ),( Xiang Hua Tang ),( Yun Juan Yang ),( Jun Mei Ding ),( Nan Yu Han ),( Zun Xi Huang 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1
Xylanases sourced from different bacteria have significantly different enzymatic properties. Therefore, studying xylanases from different bacteria is important to their applications in different fields. A potential xylanase degradation gene in Massilia was recently discovered through genomic sequencing. However, its xylanase activity remains unexplored. This paper is the first to report a xylanase (XynRBM26) belonging to the glycosyl hydrolase family (GH10) from the genus Massilia. The gene encodes a 383-residue polypeptide (XynRBM26) with the highest identity of 62% with the endoxylanase from uncultured bacterium BLR13. The XynRBM26 expressed in Escherichia coli BL21 is a monomer with a molecular mass of 45.0 kDa. According to enzymatic characteristic analysis, pH 5.5 is the most appropriate for XynRBM26, which could maintain more than 90% activity between pH 5.0 and 8.0. Moreover, XynRBM26 is stable at 37°C and could maintain at least 96% activity after being placed at 37°C for 1 h. This paper is the first to report that GH10 xylanase in an animal gastrointestinal tract (GIT) has salt tolerance, which could maintain 86% activity in 5 M NaCl. Under the optimum conditions, Km, Vmax, and kcat of XynRBM26 to beechwood xylan are 9.49 mg/ml, 65.79 μmol/min/mg, and 47.34 /sec, respectively. Considering that XynRBM26 comes from an animal GIT, this xylanase has potential application in feedstuff. Moreover, XynRBM26 is applicable to high-salt food and seafood processing, as well as other high-salt environmental biotechnological fields, because of its high catalytic activity in high-concentration NaCl.