FSCB phosphorylation in mouse spermatozoa capacitation

( Shun Li Liu ),( Bing Ni ),( Xiang Wei Wang ),( Wen Qian Huo ),( Jun Zhang ),( Zhi Qiang Tian ),( Ze Min Huang ),( Yi Tian ),( Jun Tang ),( Yan Hua Zheng ),( Feng Shuo Jin ),( Yan Feng Li ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.8

It is generally accepted that spermatozoa capacitation is associated with protein kinase A-mediated tyrosine phosphorylation. In our previous study, we identified the fibrous sheath CABYR binding protein (FSCB), which was phosphorylated by PKA. However, the phosphorylation status of FSCB protein during spermatozoa capacitation should be further investigated. To this aim, in this study, we found that phosphorylation of this 270-kDa protein occurred as early as 1 min after mouse spermatozoa capacitation, which increased over time and remained stable after 60 min. Immunoprecipitation assays demonstrated that the tyrosine and Ser/Thr phosphorylation of FSCB occurred during spermatozoa capacitation. The extent of phosphorylation and was closely associated with the PKA activity and spermatozoa motility characteristics. FSCB phosphorylation could be induced by PKA agonist DB-cAMP, but was blocked by PKA antagonist H-89.Therefore, FSCB contributes to spermatozoa capacitation in a tyrosine-phosphorylated format, which may help in further elucidating the molecular mechanism of spermatozoa capacitation. [BMB reports 2011; 44(8): 541-546]

Similarities and Differences in East Asian Confucian Culture: A Comparative Analysis

Min-hua Huang,Shu-Hsien Chang 숙명여자대학교 아시아여성연구원 2017 OMNES: The Journal of Multicultural Society Vol.7 No.2

In East Asia, Confucianism has long been regarded as the cultural basis for social norms and political order. In the past literature, many scholars have argued that the special nature of Confucian culture is an important factor hindering the modernization of East Asian countries. However, rapid modernization in the past two decades has shown that East Asia is not limited in its modernization by its cultural features. In this paper, the authors explore how the effects of modernization and democratization affect Confucian values in East Asian. Using data from the latest wave of the Asian Barometer Survey data in nine East and Southeast Asian countries, the authors find that modernization and democratization might attenuate Confucianism, but this trend is actually transformative instead of linear, meaning that Confucianism may be transformed into a post-modern culture that offers spiritual well-being and continues to guide the future progress of human civilization.

How Does Rising Internet Usage Affect Political Participation in East Asia? Explaining Divergent Effects

( Min-hua Huang ),( Ching-hsuan Su ),( Ruixia Han ),( Mark Weatherall ) 경남대학교 극동문제연구소 2017 ASIAN PERSPECTIVE Vol.41 No.4

The advance of information and communication technologies (ICTs) has fundamentally changed the way people communicate and interact with each other, and the rise of the Internet profoundly affects political participation. In applying the latest Asian Barometer surveys, we discovered Internet-driven divergent effects on political participation, suggesting rising Internet usage is simultaneously associated with decreasing electoral and increasing activist participation. Further analysis revealed that the divergent effects can be explained by severe frustration with the political system and economic conditions. When considering state polity characteristics, we found that the Internet-driven divergent effects in a democratic context refer to a coincidence of two Internet-related effects that decrease electoral and increase activist participation; in an authoritarian context, they indicate a correlation between greater Internet usage and a preference for activist over electoral participation.

Democratic Distance and Asian Views of Chinese and American Influence

Min-Hua Huang,Mark Weatherall 동아시아재단 2017 Global Asia Vol.12 No.1

Digital Revolution or Digital Dominance? Regime Type, Internet Control, and Political Activism in East Asia

Min-Hua Huang,Wen Hong 한국국제정치학회 2017 The Korean Journal of International Studies Vol.15 No.2

MRAS Genetic Variation is Associated with Atherothrombotic Stroke in the Han Chinese Population

Hua Liu,Xiao Qin Huang,Min Yang,Xun Ming Ji,Xin Du,Jian Zheng 대한신경과학회 2013 Journal of Clinical Neurology Vol.9 No.4

Background and Purpose Atherothrombotic cerebral infarction [atherothrombotic stroke (ATS)] shares common risk factors and pathophysiological mechanisms with coronary artery disease (CAD), and both diseases appear to have common susceptibility loci. The muscle RAS oncogene homolog gene (MRAS) has been identified as a susceptibility locus for CAD and is implicated in atherosclerosis. The aim of this study was to elucidate whether the single-nucleotide polymorphisms (SNPs) and haplotypes of MRAS are associated with increased risk of ATS in a population of Han Chinese. Methods A case-controlled association study was conducted in which only patients with ATS (identified as a major subtype in the Korean modification of the Trial of Org 10172 in Acute Stroke Treatment classification) were enrolled. Subgroup analyses were carried out to determine whether the effect of the MRAS polymorphism was specific to age and gender among the subjects. Results In total, 194 ATS and 186 control subjects were included in the present study. Two tagging SNPs were identified in MRAS (rs40593 and rs3755751). A multivariate regression analysis revealed a positive association between rs40593 and ATS under dominant and additive models after adjustment for covariates. Subgroup analyses revealed that there were no gender differences with respect to allele or genotype frequencies between the groups. The AG genotype for rs40593(p=0.028), the CT genotype for rs3755751 (p=0.036), and G-allele carriers (AG plus GG) for rs40593 (p=0.015) exhibited a significant protective effect among those aged ≥45 years. For the haplotype analysis, ATS subjects aged ≥45 years had a higher frequency of the ACAC haplotype (76.0%) than the controls (68.1%; p<0.05); that haplotype was associated with an increased risk of ATS. Conclusions The obtained data suggest a positive association between MRAS and ATS among the Han Chinese. Further studies should be performed with larger sample and among different ethnic populations, and gene-gene or gene-environment interactions should be considered.

The cytoplasmic loops of AgrC contribute to the quorum-sensing activity of Staphylococcus aureus

Huang Qian,Xie Yihui,Yang Ziyu,Cheng Danhong,He Lei,Wang Hua,Liu Qian,Li Min 한국미생물학회 2021 The journal of microbiology Vol.59 No.1

In Staphylococcus aureus, the accessory gene regulator (agr) quorum-sensing system is thought to play an important role in biofilm formation. The histidine kinase AgrC is one of the agr system components and activated by the self-generated auto-inducing peptide (AIP), which is released continuously into the extracellular environment during bacterial growth. The extracellular loops (Extra-loops) of AgrC are crucial for AIP binding. Here, we reported that the cytoplasmic loops (Cyto-loops) of AgrC are also involved in Agr activity. We identified S. aureus ST398 clinical isolates containing a naturally occurring single amino acid substitution (lysine to isoleucine) at position 73 of an AgrC Cyto-loop that exhibited significantly stronger biofilm formation and decreased Agr activity compared to the wild-type strain. A constructed strain containing the K73I point mutation in AgrC Cyto-loop continued to show a growth dependent induction of the agr system, although the growth dependent induction was delayed by about 6 h compared to the wild-type. In addition, a series of strains containing deletion mutants of the AgrC Cyto- and Extra-loops were constructed and revealed that the removal of the two Cyto-loops and Extra-loops 2 and 3 totally abolished the Agr activity and the growth-dependence on the agr system induction. Remarkably, the Extra-loop 1 deletion did not affect the Agr activity. In conclusion, the AgrC Cyto-loops play a crucial role in the S. aureus quorum-sensing activity.

Research on Gateway Deployment Solving Model Applied in the Wireless Mesh Network

HUANG Jian-Heng,YU Jun-Qi,ZHAO Min-Hua 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.3

In order to solve gateway deployment of geometry K center in the wireless network and improve the service quality of network, this paper proposes the gateway deployment solving model and algorithm. This paper transforms center problem of the geometric K into center problem of node K and solves problem. This paper proposes a gateway node alternative selection algorithm combination of network topology properties and maximum cover area related theory, and according to the relevant features select an alternative area, each alternative area abstract for a virtual node and is inserted into the original network topology structure, thus forming the new network topology. Solving new problem of the network topology and looking for the optimal deployment scheme use the improved genetic algorithm. The experimental results show that the model and algorithm proposed in this paper can well solve the wireless network gateway deployment problem of geometric K center and improve the network service quality.

Social Network Partition Method based on Concentricity-Controllable Routing

Huang Jian-Heng,Yu Jun-Qi,Zhao Min-Hua 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.4

In allusion to issues that partitioning algorithm of traditional social network community is generally lacking in comprehensive consideration of node and link attributes as well as full expression of model and mechanism using attributes of node and link. This paper presents a social network community partitioning algorithm fusing node and link attributes. The algorithm has blended similarity of node properties, link weights between nodes and link attribute information, making definition of the similar weights, and on this basis, social networking community division is realized combined with condensation algorithms. Experiments show that the algorithm has remarkable effects on community partition with obvious attributes in social network.

Palladium nanoparticles decorated mesoporous carbon spheres as catalyst for reduction of 4-nitrophenol.

Huang, Xin Hua,Moon, Byeong Kyu,Byeon, Seong Jin,Heo, Min Seon,Kim, Ii American Scientific Publishers 2014 Journal of nanoscience and nanotechnology Vol.14 No.11

<P>Two kinds of polyaromatics with mesoporous have been synthesized from aromatic hydrocarbons using anhydrous zinc chloride as the Friedel-Crafts catalyst and chloromethyl methyl ether as a cross-linker, after the Pd nanoparticles (PdNPs) decorated on the mesoporous carbon spheres (Pd@CSs) have been prepared by simply mixing the as-prepared polyaromatics (polynaphthalene or polypyrene) with PdCl2, reducing the Pd2+ to Pd0 by using NaBH4, followed by thermal treatment at 600 degrees C for 5 h in nitrogen atmosphere. The synthesized PdNPs have a uniform size distribution with an average size smaller than 15 nm and they can be loaded on the highly mesoporous carbon microspheres. Structural of the resulting Pd@CSs were carried out using FE-SEM, HR-TEM, X-ray differaction, dispersive X-ray spectroscopy. The resulting Pd@CSs have been investigated as a catalyst for the reduction of 4-nitrophenol to 4-aminophenol, showing the Pd@CSs have high catalytic reactivity and recyclability.</P>