수확 후 산채류의 미생물 제어를 위한 이산화염소수와 유기산 및 Blanching 병합 처리

강지훈(Ji Hoon Kang),박신민(Shin Min Park),김현규(Hyun Gyu Kim),손현정(Hyun Jung Son),이가연(Ka Yeon Lee),강길남(Kil-Nam Kang),박종태(Jong Tae Park),송경빈(Kyung Bin Song) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.2

수확 후 산채류의 미생물학적 안전성을 확보하기 위해 선정된 산채류인 취나물과 곤드레에 이산화염소수와 유기산 용액 병합 처리 및 이산화염소수, 유기산 용액, blanching 병합처리 후 미생물 제어 효과를 비교 분석하였다. 50 ppm 이산화염소수와 0.5% citric acid 용액의 병합 처리는 취나물과 곤드레의 총 호기성 세균 수를 2.80~3.64 log CFU/g, 효모 및 곰팡이 수는 2.02~2.67 log CFU/g 감소시켰다. 50 ppm이산화염소수와 0.5% fumaric acid 용액의 병합 처리 후 총 호기성 세균 수는 대조구와 비교하여 3.62~3.82 log CFU/g 감소하였으며, 효모 및 곰팡이의 경우에는 2.47~3.02 log CFU/g 만큼 감소하여 이산화염소수와 citric acid 용액의 병합 처리보다 fumaric acid 용액과의 병합 처리가 더 효과적인 병합 처리 조건이라고 생각된다. 이산화염소수와 fumaric acid 병합 처리 후 blanching 처리된 취나물의 총 호기성 세균 수는 대조구보다 5.12 log CFU/g 더 낮게 검출되었으며, 효모 및 곰팡이는 검출되지 않았다. 곤드레의 경우에도 효모 및 곰팡이는 검출되지 않았으며, 총 호기성 세균 수는 대조구와 비교하여 4.59 log CFU/ g 감소하였다. 따라서 본 연구 결과 이산화염소수와 유기산 용액 전처리 후 blanching 병합 처리가 산채류의 미생물학적 안전성을 확보하는 가장 효과적인 방법이라고 판단된다. To improve the microbiological safety of wild vegetables after harvest, Aster scaber and Cirsium setidens Nakai were treated with combinations of 50 ppm aqueous chlorine dioxide (ClO₂)/0.5% citric acid or fumaric acid, and 50 ppm ClO₂/0.5% fumaric acid/blanching at 90°C for 2 min. Combined treatment of 50 ppm ClO₂ and 0.5% citric acid reduced populations of total aerobic bacteria, yeast, and molds in Aster scaber and Cirsium setidens Nakai by 2.80∼3.64 and 2.02∼2.67 log CFU/g, respectively, compared to those of the control. Combined treatment of 50 ppm ClO₂ and 0.5% fumaric acid reduced total aerobic bacteria, yeast and molds populations by 3.62∼3.82 and 2.47∼3.02 log CFU/g, respectively. Based on the results, combined treatment of ClO₂ and fumaric acid was more effective in controlling microorganisms in the wild vegetables than either ClO₂ or citric acid. In addition, combined treatment of ClO₂/fumaric acid/blanching reduced the populations of total aerobic bacteria by 4.59∼5.12 log CFU/g, and populations of yeast and molds were not detected by treatment. These results suggest that combined treatment of ClO₂/fumaric acid/blanching is the most effective method for improving microbiological safety of wild vegetables after harvest.

Purification and Characterization of Helicobacter pylori ${\gamma}$-Glutamyltranspeptidase

Song, Jae-Young,Choi, Yeo-Jeong,Kim, Jeong-Min,Kim, Yoo-Ree,Jo, Jin-Seong,Park, Jin-Sik,Park, Hee-Jin,Song, Yun-Gyu,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Youn, Hee-Shang,Cho, Myung-Je,Rhee, Kw The Korean Society for Microbiology 2011 Journal of Bacteriology and Virology Vol.41 No.4

Gamma-glutamyltranspeptidase (GGT) was purified to electrophoretic homogeneity from the cell extract of H. pylori. The purified enzyme consisted of heavy and light subunits with molecular weights of 38 kDa and 21 kDa, respectively. N-terminal amino acid sequence of heavy and light subunits revealed that H. pylori GGT was processed into 3 parts for a signal peptide of 27 amino acid residues, a heavy subunit of 352 residues, and a light subunit of 188 residues during translation. The reaction rate for hydrolysis of ${\gamma}$-GpNA was 84.4 ${\mu}mol/min$ per milligram of protein, and that for the ${\gamma}$-glutamyl transfer from ${\gamma}$-GpNA to gly-gly was 23.8 ${\mu}mol/min$ per milligram of protein. The apparent Km values of H. pylori GGT for ${\gamma}$-glutamyl compounds were on the order of $10^{-3}$ to $10^{-4}$ M and those for acceptor peptides and amino acids were on the order of $10^{-1}$ to $10^{-2}$ M. The GGT protein kept approximately 80% of the initial enzymatic activity on incubation at $60^{\circ}C$ for 15 min. The optimum temperature and pH for reactions of both hydrolysis and transpeptidation were $40^{\circ}C$ and 9.0, respectively. The transpeptidation and hydrolysis reactions catalyzed by H. pylori GGT were strongly inhibited by L-Gln and moderately inhibited by L-Ala, L-Ser, ${\beta}$-chloro-L-Ala, and L-Glu. These results demonstrated that the biochemical properties of H. pylori GGT are different from those of other bacterial GGTs. Further, H. pylori GGT might degrade glutathione in the gastric mucous layer of humans if the enzyme could be secreted in the bacterial niches.

수근관 증후군의 국소 스테로이드 주사

조민규,이성훈,강은영,전평식,최영태 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.5

신증후성 출혈열(HFRS)의 임상 양상에 관한 고찰

강민규,황평주,김종학,나기량,이강욱,신영태 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Hemorrhagic fever with renal syndrome(HFRS) is an acute viral disease characterized by fever, cardiovascular instabilities, hemorrhagic manifestation and renal failure. Eighty-eight cases of HERS who were admitted to Chung-Nam National University Hospital from July 1988 to December 1997 were analysed from the stand-point of clinical and laboratory findings. The results were as follows. 1. The months of peak incidence were November and December. 2. The most frequent chief complains on admission were fever and chill, abdominal pain, nausea and vomiting, myalgia and headache, respectively. 3. The clinical stage on admission were oligouric(42 cases), febrile(22 cases), hypotensive(5 cases) and diuretic(3 cases) phase, respectively. 4. In laboratory analysis, leukocytosis, thrombocytopenia and proteinuria were observed in most of the case. 5. The infection rate of Leptospirosis and Scrub typhus in 72 HFRS patients was 13.9%(8 cases and 2 cases). 6. The mortality rate was 4.2% anti the cause of death were shock(2cases) and pulmonary hemorrhage(1 case)

生物敎科書에 나오는 植物種類와 鎭海市 初·中·高等學校 庭園樹木과의 比較

姜東萬,鄭宇珪,成敏雄 慶尙大學校 기초과학연구소 1989 基礎科學硏究所報 Vol.5 No.-

학교 정원의 교재원화를 위한 기초조사로써 1984년 3월부터 1988년 10월까지 국민학교 자연, 중학교 과학, 고등학교 생물 교과서에 출현하는 관다발식물과 진해시내 19개 초·중·고등학교의 정원에 식재되어 있는 수목의 종류와 빈도 등을 조사한 결과는 다음과 같았다. 1. 생물 교과서에 출현하는 전체 관다발 식물은 91과 182속 192종 21변종 1품종 총 214종이었다. 이들 중에는 국민학교 33종 중학교 63종 고등학교에 208종이 포함되었다. 생물 교과서에 나오는 목본 식물은 전체 37과, 58속, 68종, 8변종 1품종 총 77종이었고 이들 중에는 국민학교 8종, 중학교 22종, 고등학교에 73종이 포함되었다. 2. 생물 교과서에 나오는 관다발 식물의 총빈도는 1576이고 국민학교 238, 중학교 268, 고등학교 1074였다. 종당 평균 출현회수는 전체 74회, 국민학교 7.2회, 중학교 7.2회 고등학교 2.5회였다. 3. 진해시내 19개 학교 정원에 식재되어 있는 수목은 전체 53과 96속 126종 33변종 6품종 총 165종이었고 10개 국민학교에 139종, 4개 중학교에 77종, 5개 고등학교에 114종이 식재되어 있었다. 4. 10개교에 식재된 종의 총빈도는 1019이고 종당 평균빈도는 6.2였다. 전체 학교에 식재된 수목은 개입깔나무, 가이쓰카 향나무, 둥근향나무, 장미, 회양목, 무궁화, 사철나무, 개나리의 8종이고 은행, 편백, 서리화백, 측백, 두꺼운 잎유카, 버짐나무, 왕벚나무, 꽝꽝나무, 단풍나무, 동백, 아왜나무 11종이 빈도 80%이상이었다. 5. 19개교의 총개체수는 24531주, 학교당 평균 개체수는 1291주, 국민학교 1562주, 중학교 1445.5주, 고등학교 625.8주였다. 종당 평균 개체수는 전체학교에서 148.7주, 국민학교 112.4주, 중학교 75주, 고등학교 27.4주였다. 1000주 이상 식재된 수목은 측백(4075), 개나리(1986), 사철나무(1915), 연산홍(1730), 무궁화(1509), 편백(1295), 둥근향나무(1112), 회양목(1043)이었고 이들 8종이 전체의 43.2%를 차지하였다. 나자식물이 종수에 있어 21.2%, 개체수에 있어 43.0% 였고 상록수가 종수에 있어 43.0%, 개체수에 있어 74.2%였다. 6. 19개교의 총종수는 165종이고 학교당 평균 종수는 53.4종이었고 국민학교 59.8종, 중학교 37.2종 , 고등학교 53.6종이었다. 가장 종수가 많은 학교는 진해 고등학교로 84종이었고, 가장 적은 학교는 진해종합고등학교로 21종이었다. 7. 진해시 학교 정원에 식재된 수목 165종중 생물 교과서에 나오는 수종은 46종으로 관다발 식물의 21.0%, 수목의 59.7%였다. 그러나 생물 교과서에 나오는 식물중에서 학교 정원에 식재되어 있는 학교당 평균 종수는 국민학교 4.6종, 중학교 3.2종, 고등학교 12종이었다. 이 수치는 생물 교과서에 나오는 목본식물의 수에 각각 57.5%, 14.1%, 16.4% 였고, 관다발식물의 수에 비해 각각 13.9%, 5.1%, 5.8%에 해당하였다. 8. 진해시 학교 정원에 식재된 수목은 교육적 기능보다는 관상 위주로 식재되었고 침엽수와 상록수가 지나치게 많이 식재되어 적절한 조정이 필요하다. This paper was carried out to investigate the kinds of vascular plants and their frequencies appearing in the books of the elementary school; "wise life" and "nature". in the books of the high school; "biology". These investigations were compared the kinds of the garden trees and their frequencies of 10 elementary. 4 middle. and 5 high schools and total 19 schools in chinhae city and the vasular plants writtern in the biology text books as a basic research for the teaching gardenization of school gardens from March. 1984 to August. 1988. The results were summarized as follows: 1. The biology text books appeared total 214 kinds of the vascular plants. According to classification, they were 91 families, 182 genera, 192 species, 21 varieties , and 1 forma. These consisted of 33 kinds in the books of the elementary school; "wise life" and "nature " , 63 kinds in the books of the middle school; "general science", and 208 kinds in the books of the high school; "biology". In all the biology text books the woody plants were total 77 kinds or 37 families, 58 genera, 68species, 8 varieties, and 1 form. Among them, the woody plants of 8 kinds in the elementary school, 22 kinds in the middle school, and 73 kinds in the high school were contained. 2. Total frequency of vascular plants appearing in the biology text books was 1576 times. Among them, the vascular plants of 238 times in the elementary school, 268 times in the middle school, and 1074 times in the high school appeared. Average frequency per species was 74 times in total species of all the biology text books, 7.2 times in the wise life and nature text books of the elementary school, 7.2 times in the general science text books of the middle school, 2.5 times in the biology text books of the high school. 3. The woody plants in school garden in Chinhae city were 165 kinds. According to classification, they were 53 families, 96 genera, 126 species, 33 varieties, and 6 forma in the gardens of total 19 schools. The woody plants of 139 kinds in the gardens of 10 elementary schools, 77 kinds in the gardens of 4 middle schools, and 114 kinds in the gardens of 5 high schools were planted. 4. Total frequency of kinds planted in 19 schools and average frequency per species was 1019 times 6.2 times. The woody plants appearing in the garden of schools were 8 kinds of Cedrus deodara, Juniperus chinensis var. kaizuka, J. chinensis var. globosa, Rosa hybrida. Puxus microphylla var, koreana, Hibiscus syriacus, Euonymus japonca, and Forsythia koreana, 11 kinds of Ginkgo biloba, Chamaecyparis obtus, C. pisifera var. squarrosa, Thuja orientalis, Yucca recurrifolia, Platanus orientalis, Prunus yedoensis, Ilex crenata var. microphylla, Acerplantanum var. palmatum, Camella japonica, and Viburnum aieabuki were 80% or over in frequency appearing in the gardens of 19 schools. 5. Total individuals appearing in the gardens of 19 schools were 24531 trees. Average individual number per school was 1291 trees in all the schools, 1562 trees in the elementary schools, 14455 trees in the middle schools, and 625.8 in the high schools. Average individual number per species was 148.7 trees in all the schools, 112.4 trees in the elementary schools, 75 trees in the middle school and 27.4 trees in the high schools. The greatest individual number among 19 schools in Chinhae city was 3994 in Daeya elementary school. The smallest individual number among 19 schools in Chinhae city was 271 in Chinhae comprehensive senior high school. 6. The woody plants appearing 1000 trees or over in the gardens of 19 schools were T. orientalis(4075), F. koreana(1986), E. japonica(1915), Rhododendron obusum(1730), H.syriacus(1509), C. obtus(1294), J.chinensis var. globosa(1112), and P. microphylla var. koreana(1043). These 8 species were 43.2% of total individual number. The ever green trees appeared 43.0% in the species numbers and 74.2% in the individual number. 7. The woody plants in all the school garden were 165 species in total. Average species number in each school were 53.4 species in total schools, 59.8 species in the elementary schools, 37.2 species in the middle schools, and 53.6species in the high schools. The greatest species number among 19 schools in Chinhae city was 84 species in Chinhae senior high school. The smallest species number among 19 schools was 21 species in Chinhae comprehensive senior high school. The woody plants appearing in the biology text books among 165 species growing in the school gardens in Chinhae city were 46 species. These species numbers were under 21.0% of vascular plants and 59.7%of woody plants appearing in the biology text books. These species number of the woody plants growing in 19 school gardens among the woody plants appearing in the biology text books were 4.6 species in the elementary schools, 3.2 species in the middle schools, and 12 species in the high schools. These species numbers were under 57.7%, 14.1%, and 16.4% in the numbers of woody plants of the biology text books and 13.9%, 5.1% and 5.8% in the number of vascular plants of the biology text books. 8. The authors could find out that the plants planted in the school garden were not suitable to use for the teaching material plants because of planting only the woody plants of school garden for landscape rather than plants for teaching materials. Therefore some of the improvement for the teaching material garden of school were issused in the discussion.

버섯중 철이온에 활성화된 광감응성 Mitochondrial ATPase에 관한 연구

민태진,이미애,배강규 동국대학교 자연과학연구소 1993 자연과학연구 논문집 Vol.13 No.-

표고버섯중 광감응성 mitochondrial F?-ATPase는 Fe?, Fe? 및 M? 이온에 의하여 각각 활성화 되었으며 5.0mM Fe? 이온에 의한 상대활성도는 대조구에 비하여 107% 증가되었다. Mg? 존재하에서 Fe? 및 Fe? 각 이온 농도효과는 모두 효소의 활성을 증가 시켰으나 0.1mM Mg?과 5.0mM Fe? 이온의 공존하에서 170%를 증가시켜 Mg? 이온에 의한 상승작용을 보였다. 0.1mM Mg?과 0.1mM Fe? 존재하에서 Fe? 이온 농도효과는 그 농도가 5.0mM일 때 168%의 활성도 증가를 보여 Fe? 이온 공존 효과는 없었다. 이 효소는 Mg? 및 Fe? 이온에 으하여 활성화되는 특성을 가지고 있으며 활성 금속이온 존재하에서 측정한 최적 pH 및 온도는 각 각 7.5 및 66℃였다. The effects of the iron on the light-induced mitochondrial F?-ATPase in Lentinus edodes was studied. This enzyme activity was stimulated by each of the ferric, ferrous and magnesium ion. Especially, the activity of the enzyme by 5.0mF ferric ion increased up to 107% in comparision with control group(100%). In the presence of magnesium ion, each of ferric and ferrous ion increased the activity of the enzyme, particulary, coexistence of 0.1mM magnesium and 5.0mM ferric ion increased the activity up to 270% with magnesium ion dependence. The activity of the enzyme was stimulated up to 268% by 5.0mM ferric ionin the presence of 0.1mM magnesium and 0.1mM ferrous ion. Therefore, the coexistence of ferrous ion did not affect the activity. From the above, we propose that the light-induced mitochondrial F?-ATPase in L. edodes is a Mg?·Fe? f?-ATPase. The optimal pH and temperature for the enzyme were 7.5 and 66℃, respectively.

선박용 폐 FRP 수지의 재활용 공정 개발

강세란,김영우,황덕기,김시영,이민규,주창식 한국환경과학회 2003 한국환경과학회지 Vol.12 No.6

A recycling process for the waste FRP from boats was developed. The recycling process is composed of decomposition of waste FRP with propylene glycol and synthesis of recycled unsaturated polyester resin from the decomposed liquid material. Prior to the decomposition, waste FRP was cut into 2cm × 5cm segments and mechanical impact was applied by press roller to give gaps between cumulated laminates. Propylene glycol effectively decomposed the waste FRP segments and glass fibers were easily separated from decomposed liquid material. Recycled unsaturated polyester resin could be made from the decomposed liquid material by reaction with maleic anhydride and phthalic anhydride.

초임계 이산화탄소를 이용하는 친환경 공정에 의한 다공성 고분자의 제조

강세란,홍성수,이민규,이석희,천재기,주창식 한국환경과학회 2004 한국환경과학회지 Vol.13 No.3

An experimental study on the preparation of monolithic porous polymers by environmentally friend process in supercritical carbon dioxide has been carried out. Polymerization mixture composed of a cross-linking monomer, initiator and functional co-polymer was charged in the reactor with sapphire window. After the system was purged with a flow of CO₂ for 15 min, the reactor was pressurized with liquid CO₂ up to 100 bars. The reactor was isolated from and placed back to the system via quick connector for shaking until the mixture had become fully homogeneous. The reactor was then heated and pressurized to the required reaction conditions and left overnight. After cooling and CO₂ evacuation, the polymer was removed from the reactor as dry, white, continuous monoliths. The effect of experimental conditions on the physical properties of porous polymer was systematically examined, and it was found that monomer content had a major effect on the physical properties of the polymers.

濾過法에 있어서 濾材(송이) 크기에 따른 褐藻類 抽出 廢水의 處理效果

姜永周,李民圭,吳泰文 제주대학교 1990 논문집 Vol.30 No.-

Efficient utilization of the scoria which are naturally abundant in parasitic volcano area of Cheju-do was tried to be linked with purification of the waste water contaminiating environment. A packed column for waste treatment was manufactured using scoria as medium, and the used waste water was the extract of a brown alga (Ecklonia cava) which is a raw material of sodium alginate. In this study the removal efficiency of suspended solid(SS), biological oxygen demand(BOD), and chemical oxygen demand(COD) was investigated under different conditions of medium size and operation time, The following results were obtained. 1. The smaller size of the medium gave the higher efficiency of water treatment. 2. The removal efficiency of SS was higher than that of BOD or COD at specific medium size. 3. The scoria was more effective on the removal of undissolved solid than dissolved one. 4. Filtration ability of the packed column was not decreased even after 5 day' continuous operation

다리뇌 경색의 단독증상으로 발생한 핵간 눈근육 마비 1예

박강민,배종석,김상진,김응규 인제대학교 2008 仁濟醫學 Vol.29 No.-

Internuclear ophthalmoplegia (INO) is caused by a lesion involving the medial longitudinal fasciculus (MLF) that interconnects the ocular motor nuclei. A 65-year-old woman with diabetes for twenty years developed diplopia, nausea, and vomiting one day before the presentation to the emergency room. Examination revealed adduction impairment of the right eye, combined with dissociated abducting nystagmus in the left eye. Other findings of the neuro-logical examination were normal. T2-weighted brain magnetic resonance imaging showed a hyperintensity lesion in right medial pons where the MLF locates. INO may be an isolated manifestation of pontine infarction.