Antioxidant effects of citrus pomace extracts processed by super-heated steam

Wang, Lei,Jo, Min-Ji,Katagiri, Riho,Harata, Kaori,Ohta, Moemi,Ogawa, Ayane,Kamegai, Masayuki,Ishida, Yasuyuki,Tanoue, Shota,Kimura, Sojiro,Lee, Seung-Cheol,Jeon, You-Jin Elsevier 2018 FOOD SCIENCE AND TECHNOLOGY -ZURICH- Vol.90 No.-

<P>This study was performed to investigate the enhanced antioxidant effects of citrus pomace (CP) extracts processed by super-heated steam (SHS). CP extracts were obtained using a closed SHS system at three different temperatures (100, 200, and 300 degrees C) with an extraction time of 10 or 20 min. These extracts were evaluated for their antioxidant activities by measuring their DPPH, alkyl, and hydroxyl radical scavenging activity using Electron Spin Resonance (ESR) spectroscopy and for their protective effects against H2O2-induced oxidative stress in Vero cells. All extracts exhibited strong radical scavenging activities and protective effects against H2O2-induced Vero cell damage. Especially, the SHS-CP extracts obtained at 300 degrees C with extraction times of both 10 min and 20 min exhibited antioxidant activities that were significantly higher than those of ethanol extracts and SHS-CP extracts obtained at other temperatures. The total phenolic and flavonoid contents of SHS-CP extracts and their antioxidant activities were found to be increased with elevated processing temperatures. Moreover, gas chromatography/mass spectrometry (GC/MS) in the presence of a methylating reagent revealed that some characteristic polyphenols and polyhydroxycyclohexanes were present in the SHS-CP extracts. Thus, this study demonstrated that SHS extraction is an efficient and environmentally friendly method for extracting antioxidants from CP.</P>

Genome-wide analysis of the PHB gene family in Glycine max (L.) Merr.

Min Song,Xiangyong Peng,Caifu Du,Lei Lei,Tao Zhang,Yang Xiang 한국유전학회 2017 Genes & Genomics Vol.39 No.10

Prohibitins (PHBs) have one SPFH domain in common and present in species ranging from prokaryotes to eukaryotes. Although a number of researches on PHBs were performed in different plant species, a systematic analysis of the PHB family in soybean is still remains uncharacterized. In the present study, 24 putative PHB genes have been first systemically identified in soybean. According to phylogenetic analysis, these GmPHBs could be classified into four groups. Gene structures and motif patterns showed high levels of conservation within the phylogenetic subgroups. Several members of this family have undergone purifying selection based on Ka/Ks analysis on duplicated PHB genes in soybean. We performed microsynteny analysis across four legume species based on the comparisons among the specific regions contained in PHB genes. As a result, numerous microsyntenic gene pairs among soybean, Medicago, Lotus and Phaseolus were identified. Most soybean PHB genes exhibited different expression levels in various tissues and developmental stages through expression analysis using publicly available RNA-seq datasets. The 11 GmPHB genes from III_B subgroup were examined by qPCR for their expression in two soybean cultivar after infection by Phytophthora sojae. Besides three GmPHB genes previous reported by us, here other four genes also were rapidly induced by P. sojae infection in the resistant genotype, while induction was very weak in the susceptible genotype. The comprehensive overview of the PHB gene family in soybean genome will provide useful information for further functional analysis of the PHB gene family in soybean.

CHISELED NICKEL HYDROXIDE NANOPLATES GROWTH ON GRAPHENE SHEETS FOR LITHIUM ION BATTERIES

LEI-LEI TIAN,XIAN-YONG WEI,QUAN-CHAO ZHUANG,CHAO WU,RUI-LUN XIE,ZHI-MIN ZONG,YONG-LI CUI,SHI-GANG SUN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.6

The morphologies and structures of Ni(OH)2–graphene hybrid materials were tailored by using different mineralizers in this work. It was revealed that the synergic effects of the highly oxidized graphene sheets and the mineralizers played a crucial role in controlling the morphology and structure of the nanocomposites, and Na2CO3 is a very effective mineralizer for growing chiseled 2D nanoplates of Ni(OH)2 on graphene sheets. When produced with NaOH, fragmental Ni(OH)2 crystals with irregular shapes erratically decorated on graphene sheets. In contrast, chiseled Ni(OH)2 hexagonal nanoplates grown on graphene sheets were obtained when Na2CO3 was used as the mineralizer. These unique 2D–2D nanoarchitectures with higher contact area between the nanocrystals and graphene substrate can increase the interfacial interaction and then efficiently improve the structural stability of the composite material, thus exhibiting an enhanced Li storage capacity and excellent cycling performance of 562 mAh g-1 after the 36th cycle.

Pretreatment Thrombocytosis as a Prognostic Factor in Women with Gynecologic Malignancies: a Meta-analysis

Yu, Min,Liu, Lei,Zhang, Bing-Lan,Chen, Qi,Ma, Xue-Lei,Wu, Yu-Ke,Liang, Chun-Shui,Niu, Zhi-Min,Qin, Xin,Niu, Ting Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Background: This study was performed to analyze the prognostic implications of pretreatment or preoperative thrombocytosis in women with gynecologic malignancies. Material and Methods: We surveyed 2 medical databases, PubMed and EMBASE, to identified all relevant studies. A total of 14 (n=3,490) that evaluated the link between thrombocytosis and 5-year survival were included. REVMAN version 5.1 was used for our analysis and publication bias was evaluated using the Begg's funnel plot and tested by STATA 11.0. Risk ratios (RRs) with 95% confidence intervals (CIs) generated by the random effect model were used to assess the strength of any association. Results: 709(20.3%) of the 3,490 patients exhibited thrombocytosis (platelet counts > $400{\times}10^9/L$) at primary diagnosis, and their mortality was 1.62-fold higher compared with the others (RR=1.62, 95%CI=[1.28-2.05], p<0.0001). Thrombocytosis failed to have a stronger effect on the survival of advanced patients of stages III to IV in our study (n=478, RR=1.29, 95% CI=[1.13-1.48], p=0.0003), nor in women with cervical cancer in stage IB (n=1371, RR=1.73, 95% CI=[1.71-2.58], p=0.007). In addition, when adjusted for different carcinoma, it was associated with worse prognosis for all except the ones with vulvar cancer (n=201, RR=0.43, 95% CI=[0.14-1.29], p=0.13). Conclusions: This meta-analysis indicated that thrombocytosis might be associated with a worse prognosis for patients with gynecologic malignancies but without specificity or sensitivity for the ones in advanced stage. When adjusted for different gynecologic malignancies, it showed a significant effect on survival of all except vulvar cancers.

Preparation and Characterization of Disorderly PCL Crystal Lamellae Electrostatic Direct Writing Scaffolds with Polydopamine Coating

Jiaqi Zeng,Wenchao Li,Min Lei,Chunfa Dong,Kui Zhou 한국섬유공학회 2023 Fibers and polymers Vol.24 No.10

Polycaprolactone (PCL) exhibits limited applicability in the application of biological tissue engineering scaffolds due to its lower surface hydrophilicity and surface energy. In this paper, PCL crystal lamellae scaffolds with different surface roughness were fabricated by immersing electrostatic direct-written PCL scaffolds in PCL/Amyl acetate (AC) solution for 15 , 30 , 60 and 120 min, respectively, using solution incubation for crystallization. The rough scaffolds were subsequently coated with polydopamine (PDA) for 4 h, 8 h, 12 h and 16 h. Surface morphology, chemical properties and water contact angle tests were performed on both types of scaffolds. To evaluate the feasibility of the modified scaffold as a bionic scaffold, L929 mouse fibroblasts were inoculated on the surface of the scaffold and cultured for 1, 3 and 7 days. When compared to the untreated scaffolds, the surface of the scaffolds treated for 15 , 30 , 60 , and 120 min, respectively exhibited a distinct PCL crystal lamellae structure, accompanied by a significant increase in surface roughness and corresponding water contact angle elevation. In the cell experiments, the 30 min treatment group demonstrated superior cellular activity compared to the other experimental groups. The water contact angle of the PDA-modified scaffolds decreased over time with extended treatment durations, ultimately reaching 0°. In the cell experiments, the 8 h treatment scaffolds exhibited a more pronounced improvement in activity compared to the other groups. Based on these results, it can be concluded that the PDA-modified PCL crystal lamellae electrostatic direct-write scaffold promotes cell proliferation and differentiation, thereby facilitating tissue regeneration.

Diagnostic Accuracy of 18F-FDG-PET in Patients with Testicular Cancer: a Meta-analysis

Zhao, Jing-Yi,Ma, Xue-Lei,Li, Yan-Yan,Zhang, Bing-Lan,Li, Min-Min,Ma, Xue-Lei,Liu, Lei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Objective: Fluorine-18-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is a new technique for identifying different malignant tumors using different uptake values between tumor cells and normal tissues. Here we assessed the diagnostic accuracy of 18F-FDG-PET in patients with testicular cancer by pooling data of existing trials in a meta-analysis. Methods: PubMed/MEDLINE, Embase and Cochrane Central Trials databases were searched and studies published in English relating to the diagnostic value of FDG-PET for testicular cancer were collected. The summary receiver operating characteristic (SROC) curve was used to examine the FDG-PET accuracy. Results: A total of 16 studies which included 957 examinations in 807 patients (median age, 31.1 years) were analyzed. A meta-analysis was performed to combine the sensitivity and specificity and their 95% confidence intervals (CIs), from diagnostic odds ratio (DOR), positive likelihood ratios (PLR), negative likelihood ratio (NLR). SROC were derived to demonstrate the diagnostic accuracy of FDG-PET for testicular cancer. The pooled sensitivity and specificity were 0.75 (95% confidence interval (CI), 0.70-0.80) and 0.87 (95% CI, 0.84-0.89), respectively. The pooled DOR was 35.6 (95% CI, 12.9-98.3). The area under the curve (AUC) was 0.88. The pooled PLR and pooled NLR were 7.80 (95% CI, 3.73-16.3) and 0.31 (95% CI, 0.23-0.43), respectively. Conclusion: In patients with testicular cancer, 18F-FDG-PET demonstrated a high SROC area, and could be a potentially useful tool if combined with other imaging methods such as MRI and CT. Nevertheless, the literature focusing on the use of 18F-FDG-PET in this setting still remains limited.

Effects of comonomer with carboxylic group on stabilization of high molecular weight polyacrylonitrile nanofibrous copolymers

Lei, Danyun,Devarayan, Kesavan,Li, Xiang-Dan,Choi, Woong-Ki,Seo, Min-Kang,Kim, Byoung-Suhk 한국탄소학회 2014 Carbon Letters Vol.15 No.4

New precursors, poly(acrylonitrile-co-crotonic acid) (poly(AN-CA)) and poly(acrylonitrile-co-itaconic acid-co-crotonic acid) (poly(AN-IA-CA)) copolymers, for the preparation of carbon fibers, were explored in this study. The effects of comonomers with acidic groups, such as crotonic acid (CA) and/or itaconic acid (IA), on the stabilization of nanofibrous polyacrylonitrile (PAN) copolymers were studied. The extent of stabilization, evaluated by Fourier transform infrared spectroscopy, revealed that the CA comonomer could retard/control the stabilization rate of PAN, in contrast to the IA comonomer, which accelerated the stabilization process. Moreover, the synthesized PAN copolymers containing CA possessed higher Mv than those of the IA copolymers and also showed outstanding dimension stability of nanofibers during the stabilization, which may be a useful property for improving the dimensional stability of polymer composites during manufacturing.

Different Response Mechanisms of Rhizosphere Microbial Communities in Two Species of Amorphophallus to Pectobacterium carotovorum subsp. carotovorum Infection

Min Yang,Ying Qi,Jiani Liu,Penghua Gao,Feiyan Huang,Lei Yu,Hairu Chen 한국식물병리학회 2023 Plant Pathology Journal Vol.39 No.2

Soft rot is a widespread, catastrophic disease caused by Pectobacterium carotovorum subsp. carotovorum (Pcc) that severely damages the production of Amorphophallus spp. This study evaluated the rhizosphere bacterial and fungal communities in Pcc-infected and uninfected plants of two species of Amorphophallus, A. muelleri and A. konjac. Principal component analysis showed that the samples formed different clusters according to the Pcc infection status, indicating that Pcc infection can cause a large number of changes in the bacterial and fungal communities in the Amorphophallus spp. rhizosphere soil. However, the response mechanisms of A. muelleri and A. konjac are different. There was little difference in the overall microbial species composition among the four treatments, but the relative abundances of core microbiome members were significantly different. The relative abundances of Actinobacteria, Chloroflexi, Acidobacteria, Firmicutes, Bacillus, and Lysobacter were lower in infected A. konjac plants than in healthy plants; in contrast, those of infected A. muelleri plants were higher than those in healthy plants. For fungi, the relative abundances of Ascomycota and Fusarium in the rhizosphere of infected A. konjac plants were significantly higher than those of healthy plants, but those of infected A. muelleri plants were lower than those of healthy plants. The relative abundance of beneficial Penicillium fungi was lower in infected A. konjac plants than in healthy plants, and that of infected A. muelleri plants was higher than that of healthy plants. These findings can provide theoretical references for further functional research and utilization of Amorphophallus spp. rhizosphere microbial communities in the future.

Effects of Herbiotic FS supplementation on growth performance, nutrient digestibility, blood profiles, and faecal scores in weanling pigs

Lei, Xin Jian,Yun, Hyeok Min,Kang, Jung Sun,Kim, In Ho Informa UK (TaylorFrancis) 2018 Journal of applied animal research Vol.46 No.1

NiCo₂O₄ nanostructure-decorated PAN/lignin based carbon nanofiber electrodes with excellent cyclability for flexible hybrid supercapacitors

Lei, Danyun,Li, Xiang-Dan,Seo, Min-Kang,Khil, Myung-Seob,Kim, Hak-Yong,Kim, Byoung-Suhk Elsevier 2017 Polymer Vol.132 No.-

<P><B>Abstract</B></P> <P>Nanostructured binary metal oxide-decorated carbon nanofibers (CNFs) were utilized as flexible electrodes for hybrid supercapacitor applications. Polyacrylonitrile (PAN)/lignin based carbon nanofibers (PAN/lignin CNFs) with different contents of lignin were prepared by electrospinning, stabilization and carbonization. Afterwards, the NiCo<SUB>2</SUB>O<SUB>4</SUB> oxides were deposited on the surface of CNFs by a facile hydrothermal method without any toxic reagents. SEM images confirmed that the NiCo<SUB>2</SUB>O<SUB>4</SUB> (nanosheet and nanoneedle) nanostructures were uniformly grown on the surface of every carbon fiber. The obtained flexible NiCo<SUB>2</SUB>O<SUB>4</SUB>-decorated PAN/lignin CNFs with 50% lignin (NiCo<SUB>2</SUB>O<SUB>4</SUB>@CNF55) electrode exhibited high specific capacitance of the ∼1757 F g<SUP>−1</SUP> at 2 mA cm<SUP>−2</SUP> and excellent cyclability with ∼138% capacitance retention after 5000 cycles at 7 mA cm<SUP>−2</SUP>, suggesting lower internal resistance and higher electrochemical reversibility. In addition, the NiCo<SUB>2</SUB>O<SUB>4</SUB>@CNFs//N-rGO solid state asymmetric supercapacitor devices were assembled in order to evaluate their practical applications. The NiCo<SUB>2</SUB>O<SUB>4</SUB>@CNF55//N-rGO asymmetric supercapacitor device exhibited a specific capacitance of 134.3 F g<SUP>−1</SUP> at a current densities of 1 A g<SUP>−1</SUP>, and possessed a maximum energy density of 47.75 Wh kg<SUP>−1</SUP> with a power density of 799.53 W kg<SUP>−1</SUP>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PAN/lignin-based carbon fibers were prepared by electrospinning, stabilization and carbonization. </LI> <LI> The NiCo<SUB>2</SUB>O<SUB>4</SUB> nanostructures were fabricated on PAN/lignin-based carbon nanofibers. </LI> <LI> The NiCo<SUB>2</SUB>O<SUB>4</SUB>-decorated fiber electrode exhibits highest specific capacitance of the ∼1757 Fg<SUP>-1</SUP>. </LI> <LI> The NiCo<SUB>2</SUB>O<SUB>4</SUB>-decorated fiber electrode shows excellent capacitance retention of ∼138% after 5000 cycles. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>