대두 및 고구마 β-Amyiase 의 비교에 관한 연구

Mikami, Bunzo,Majima, Keiichi,Morita, Yuhei,김용휘,김준평 한국콩연구회 1988 韓國콩硏究會誌 Vol.5 No.1

대두 및 고구마로부터 얻은 β-amylase의 단백질 구조를 CD spectra, 항체반응, 화학적 절단을 통하여 비교하였다. 고구마 β-amylase는 4개의 동일한 subunit로 구성되어 있으며 대두 β-amylase는 subunit구조를 하고 있지 않았다. 또한 두 효소는 변성시킨 상태에서 SDS-gel전기영동, gel filtration한 결과 분자량은 동일하였다. 그리고 대두 및 고구마 β- amylase는 CD spectra 상 유사한 2차구조를 나타내고 있으나 방향족 측쇄가 상이함을 나타냈다. 한편 cyteine 잔기 및 mechionine 잔기의 화학적 절단한 결과 두 효소는 동일한 아미노산 배열을 나타냈다. 또한 면역학적인 방법에 의해서도 두 효소는 유사성이 인정되었다. 한편 대두 β-amylase에 대한 항체는 고구마 β-amylase의 활성을 억제 하였으나 밀, 보리, 무우 β-amylase에 대해서는 활성 억제가 나타나지 않았다.

Health Monitoring of High-rise Building with Fiber Optic Sensor (SOFO)

Mikami, Takao,Nishizawa, Takao Council on Tall Building and Urban Habitat Korea 2015 International journal of high-rise buildings Vol.4 No.1

Structural health monitoring is becoming more and more important in the domain of civil engineering as a proper mean to increase and maintain the safety, especially in the land of earthquakes like Japan. In many civil structures, the deformations are the most relevant parameter to be monitored. In this context, a monitoring technology based on the use of long-gage fiber optic deformation sensor, SOFO is being applied to a 33-floors tall building in Tokyo. Sensors were installed on the $2^{nd}$ floor's steel columns of the building on May 2005 in the early stage of the construction. The installed SOFO sensors were dynamic compatible ones which enable both static and dynamic measurements. The monitoring is to be performed during the whole lifespan of the building. During the construction, static deformations of the columns had been measured on a regular basis using a reading unit for static measurement and dynamic deformation measurements were occasionally conducted using a reading unit for dynamic measurement. The building was completed on August 2006. After the completion, static and dynamic deformation measurements have been continuing. This paper describes a health monitoring technology, SOFO system which is applicable to high-rise buildings and monitoring results of a 33-floors tall building in Tokyo from May 2005 to October 2010.

Slide Session : OS-END-02 ; Diabetes : The Incidence of Diabetic Foot Ulcer on Lower Extrem-ity Amputation and All- Cause Mortality in Patients with Type 1 and 2 Diabetes in Miyoshi, Hiroshima

( Shinsuke Mikami ),( Takafumi Sugihiro ),( Satoshi Mouri ),( Yusuke Ueda ),( Hitoshi Susawa ),( Kengo Kobayashi ),( Haruki Tanaka ),( Kouichi Tanaka ),( Yukihito Higashi ),( Yasuki Kihara ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Foot ulcers are costly complication among diabetes patients. These patients have an increased risk of amputation and increased mortality rate. Early recognition of the high-risk foot and sufficient care will save legs and improve patients` quality of life. Figures of incidence of foot ulcers varies and there are only limited information in relation the change of incidence over time. The aim of this study was to estimate 5-year risk for diabetic foot ulcer (DFU), lower extremity amputation (LEA) and all cause of death. Methods: Retrospective cohort study including all subjects with diabetes enrolled in our diabetic outpatient clinic from beginning 2008 until middle 2014. Data were collected from clinical records. Results: 528 subjects with mean age of 61.3 (±13.8), 57.4% were male. The mean of HbA1c in diabetic patients at baseline were 8.1% +/- 1.9%. Cumulative incidence was 1.3% for DFU, 0.18% for LEA and 4.7% for all-cause of death. The prevalence of cardiovascular and cerebrovascular conditions, pneumonia were 2.3%, 1.9%, 2.3%, respectively. Cause of deaths was cancer (64%), pneumonia (20%), cardiovascular death (4%). Conclusions: Several factors may explain the incidence in diabetes-related LEAs. Diabetes prevention strategies and controlling risk factors are important in people with type 1 and 2 diabetes.

Designing Traffic Signal Patterns through Genetic Algorithms

Sadayoshi MIKAMI,Jun Nakajima,Yukinori KAKAZU 대한전자공학회 1992 대한전자공학회 학술대회 Vol.1992 No.10

This paper describes a new optimization technique for the design of traffic signal patterns. The proposed method uses a Genetic Algorithm for searching through the better signal patterns. Since the Genetic Algorithm is effective to search directly through a huge binary coded state spaces, the proposed design method has the following advantages over the conventional OR methods: (1) on-line optimization is available within a reasonable time, (2) there is no limitation to the types of signals to be optimized. Some computer simulations are carried out and its ability of getting high quality control in a short period is demonstrated.

Detecting Nonlinearity in Prediction Residuals of Snoring Sounds

Tsuyoshi Mikami 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8

This paper focuses on the nonlinear properties of snoring sounds for the purpose of obstructive sleep apnea diagnosis. Snoring sounds are convolutional sounds caused by wheezing of air way obstruction and oscillation of soft palate. Namely, it should be natural that the snoring sounds are generated from an onlinear dynamics, but the nonlinear properties of them have not yet been studied so far. In this paper, the nonlinearity is defined as the predictability using alinear AR prediction model, and the prediction residuals are analyzed by portmanteau test.

Efficient FPGA-based Hardware Algorithms for Approximate String Matching

Sadatoshi MIKAMI,Yosuke KAWANAKA,Shin’ichi WAKABAYASHI,Shinobu NAGAYAMA 대한전자공학회 2008 ITC-CSCC :International Technical Conference on Ci Vol.2008 No.7

In this paper, an efficient FPGA-based hardware algorithm and its extensions are proposed for calculating the edit distance as a degree of similarity between two strings. The proposed algorithms are implemented on FPGA and compared to software programs. Experimental results show the effectiveness of the proposed algorithms.

Transformation efficiency and transgene expression level in markerfree RDR6-knockdown transgenic tobacco plants

Tatsuya Mikami,Yuta Saeki,Sayaka Hirai,Mayuko Shimokawa,Yukiko Umeyama,Yusaku Kuroda,Hiroaki Kodama 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.6

RNA silencing is a sequence-specific form of epigenetic regulation that targets invasive nucleic acids. RNA-dependent RNA polymerase6 (RDR6) converts target RNA molecules, such as transgene transcripts, into double-stranded RNAs (dsRNAs) during posttranscriptional gene silencing (PTGS). Then, these dsRNAs are processed into small RNAs that guide sequencespecific RNA degradation. T-DNA-derived small RNAs are generated during the transfer of T-DNA from Agrobacterium to plant cells and compromise the function of the genes in the T-DNA. In the present study, we produced selection-markerfree transgenic tobacco plants using the MAT vector system, and expression of the tobacco RDR6 gene (NtRDR6) was suppressed using inverted-repeat-induced PTGS. Reduced expression of the NtRDR6 gene improved the transient expression of the transgene in the agroinfiltrated leaves and enhanced the production of hairy roots after infection with Agrobacterium containing a root-inducing T-DNA. The expression level of the sense transgene was determined in individual hairy roots, and knockdown of the NtRDR6 gene did not affect the distribution of the expression levels in individual transformants. These results indicate that NtRDR6 partially inhibited T-DNA function during T-DNA transfer but did not affect the expression of the transgene in stable transformants, except in transformants showing sense-transgene-induced PTGS.

The use of conization to identify and treat severe lesions among prediagnosed CIN1 and 2 patients in Japan

Mikio Mikami,Masae Ikeda,Hidetaka Sato,Haruko Iwase,Takayuki Enomoto,Yoichi Kobayashi,Hidetaka Katabuchi 대한부인종양학회 2018 Journal of Gynecologic Oncology Vol.29 No.4

OBJECTIVE: To evaluate the clinical efficiency of identifying patients with suspicious severe lesions by conization among prediagnosed cervical intraepithelial neoplasia (CIN) 1 and 2 patients in Japan. METHODS: The data in a Japanese nation-wide registry for cervical cancer (2009 and 2011) was collected to analyze the clinical efficacy of pre- and postdiagnosis for 13,215 Japanese women who underwent treatment by conization. Their preoperative and postoperative histologic findings and clinical outcomes were evaluated using standard statistical procedures including clinical and demographic characteristics. RESULTS: Almost half of 1,536 women who were treated by conization after the prediagnosis of CIN1 and 2 because the lesions showed no evidence of natural regression actually contained CIN1-2 (45.0%), CIN3 (47%), or invasive cancer (2.7%) in their cervical tissue. They underwent conization either for therapeutic (treatment) (78.5%) or diagnostic (21.5%) reasons. Invasive disease was diagnosed postoperatively more often in diagnostic cases (6.1%) than in therapeutic cases (2.8%). All the patients survived their diagnostic and therapeutic conization after approximately 30 months of follow up. CONCLUSION: Our study shows that the continuous observation of the prediagnosed CIN1 and 2 cases by the combination of cytology, colposcopy and histology in Japan has worked successfully to identify severe lesions by using conization as well in the process.

Acotiamide Has No Effects on Esophageal Motor Activity or Esophagogastric Junction Compliance

Hironobu Mikami,Norihisa Ishimura,Mayumi Okada,Daisuke Izumi,Eiko Okimoto,Shunji Ishihara,Yoshikazu Kinoshita 대한소화기 기능성질환∙운동학회 2018 Journal of Neurogastroenterology and Motility (JNM Vol.24 No.2

Background/Aims The novel prokinetic drug acotiamide is used for treatment of functional dyspepsia. It is still unclear how acotiamide has effects on esophageal motor function. Esophageal peristalsis and esophagogastric junction (EGJ) compliance has an important role for prevention of esophageal mucosal damage caused by gastroesophageal reflux, however, few studies have analyzed the effects of acotiamide on those former activities and none have investigated its effects on EGJ compliance. The aim of our research was to examine the effects of acotiamide on esophageal motility and EGJ compliance. Methods We enrolled 3 gastroesophageal reflux disease (GERD) patients as well as 9 healthy volunteers. Using high-resolution manometry, we examined esophageal motor activity parameters, including esophageal body contractions and lower esophageal sphincter (LES) pressure. While, EGJ compliance was evaluated using a functional lumen imaging probe. Following determination of baseline values for esophageal motor activities and EGJ compliance, acotiamide at a standard dose of 300 mg/day was administered for 3 days. All measurements were performed again 2 hours after the last acotiamide administration. Results In the healthy volunteers, as compared with the baseline values, acotiamide administration did not significantly change esophageal body contractions and LES pressure. And EGJ distensibility was not significantly changed (distensibility index in 40-mL distension: 3.5 ± 0.4 vs 3.3 ± 0.5 mm2/mmHg). Similarly in the GERD patients, there were no differences in either esophageal motility or EGJ compliance between before and after acotiamide administration (distensibility index in 40-mL distension: 6.2 ± 0.5 vs 6.5 ± 1.1 mm2/mmHg). Conclusion In both healthy individuals and GERD patients, standard dose acotiamide dose does not have significant effects on esophageal motor activities or EGJ compliance.

Impact of institutional accreditation by the Japan Society of Gynecologic Oncology on the treatment and survival of women with cervical cancer

Mikio Mikami,Masako Shida,Takeo Shibata,Hidetaka Katabuchi,Junzo Kigawa,Daisuke Aoki,Nobuo Yaegashi 대한부인종양학회 2018 Journal of Gynecologic Oncology Vol.29 No.2

Objective: The Japan Society of Gynecologic Oncology (JSGO) initiated a nation-wide training system for the education and certification for gynecologic oncologists in 2005. To assess the impact of the quality of the JSGO-accredited institutions, JSGO undertook an analysis of the Uterine Cervical Cancer Registry of the Japan Society of Obstetrics and Gynecology (JSOG) to determine the effectiveness of the JSGO-accredited institutions on the treatment and survival of women with cervical cancer. Methods: The effectiveness of 119 JSGO-accredited institutions and 125 non-JSGO-accredited institutions on the treatment and survival of women with cervical cancer were compared by analyzing the tumor characteristics, treatment patterns, and survival outcomes of women with stage T1B–T4 cervical cancer utilizing the data in the JSOG nation-wide registry for cervical cancer (2006–2009). Results: A total of 14,185 eligible women were identified: 10,920 (77.0%) cases for 119 JSGO-accredited institutions and 3,265 (23.0%) cases for 125 non-accredited institutions. A multivariate analysis showed that age, stage, histology type, and treatment pattern were independently associated with mortality. Moreover, women who received treatment at the JSGO-accredited institutions had a significantly decreased mortality risk compared to non-accredited institutions (adjusted hazard ratio [aHR]=0.843; 95% confidence interval [CI]=0.784–0.905). Similar findings on multivariate analysis were seen among subset of women who received surgery alone (aHR=0.552; 95% CI=0.393–0.775) and among women who received radiotherapy (aHR=0.845; 95% CI=0.766–0.931). Conclusion: Successful implementation of gynecologic oncology accrediting institution was associated with improved survival outcome of women with cervical cancer in Japan.