Serum clusterin level in children with atopic dermatitis

Sol, In Suk,Kim, Yoon Hee,Lee, Kyung Eun,Hong, Jung Yeon,Kim, Mi Na,Kim, Yun Seon,Oh, Mi Seon,Kim, Min Jung,Yoon, Seo Hee,Park, Young A.,Kim, Kyung Won,Sohn, Myung Hyun,Kim, Kyu-Earn Oceanside Publications 2016 Allergy and asthma proceedings Vol.37 No.4

Development and validation of an analytical method for the quantification of 2,6-diisopropylnaphthalene in agricultural products using GC-MS/MS

Han Sol Lee,Ji-Su Park,Su Jung Lee,Hye-Sun Shin,Yun mi Chung,Ha na Choi,Sang Soon Yun,Yong-hyun Jung,Jae-Ho Oh 한국분석과학회 2021 분석과학 Vol.34 No.1

음악중재가 비수축검사 임부의 불안과 태아심음 양상에 미치는 효과

오명옥(Oh, Myung Ok),김영점(Kim, Young Jeoum),백초희(Baek, Cho Hee),김주희(Kim, Ju Hee),박노미(Park, No Mi),유미정(Yu, Mi Jeong),송한솔(Song, Han Sol) 한국간호과학회 2016 Journal of Korean Academy of Nursing Vol.46 No.3

Purpose: The purpose of this cross-over experimental study was to examine effects of music intervention on maternal anxiety, fetal heart rate pattern and testing time during non-stress tests (NST) for antenatal fetal assessment. Methods: Sixty pregnant women within 28 to 40 gestational weeks were randomly assigned to either the experimental group (n=30) or control group (n=30). Music intervention was provided to pregnant women in the experimental group during NST. Degree of maternal anxiety and fetal heart rate pattern were our primary outcomes. State-trait anxiety inventory, blood pressure, pulse rate, and changes in peripheral skin temperature were assessed to determine the degree of maternal anxiety. Baseline fetal heart rate, frequency of acceleration in fetal heart rate, fetal movement test and testing time for reactive NST were assessed to measure the fetal heart rate pattern Results: The experimental group showed significantly lower scores in state anxiety than the control group. There were no significant differences in systolic blood pressure and pulse rate between the two groups. Baseline fetal heart rate was significantly lower in the experimental group than in the control group. Frequency of acceleration in fetal heart rate was significantly increased in the experimental group compared to the control group. There were no significant differences in fetal movement and testing time for reactive NST between the two groups. Conclusion: Present results suggest that music intervention could be an effective nursing intervention for alel viating anxiety during non-stress test.

돈피 젤라틴을 이용한 생분해성 용기의 물성과 미생물학적 안정성 및 생분해능 분석

이솔희 ( Sol-hee Lee ),정창환 ( Chang-hwan Jeong ),오유미 ( Yu-mi Oh ),김학연 ( Hack-youn Kim ) 한국산업식품공학회 2021 산업 식품공학 Vol.25 No.4

본 연구는 생분해성 용기 개발의 연구성이 대두됨에 따라서 동물성 재료로 제조한 생분해성 용기의 개발의 목적에 있다. 연구 결과 돈피, 우피, 닭피에 있어서 돈피가 우수한 수율과 단백질 분자량을 가진 것으로 나타났다. 이에 돈피를 이용하여 생분해 용기를 개발하였으며, 단면적 확인 결과 호두 껍질 분말 10%를 첨가한 처리구에서 적은 공극을 보였으며, 호두 껍질 분말 20%를 첨가한 처리구에서 공극의 크기가 큰 것을 확인할 수 있었다. 물성 연구 결과 호두 껍질 분말 10% 처리구가 더 높은 경도를 나타내었으며, 호두 껍질 분말 20% 처리구가 더 높은 탄력성을 나타내었다. 압축강도는 호두 껍질 분말 20% 처리구가 더 높은 값을 나타내었다. General bacteria, E. coli 연구 결과 모든 일자에서 불검출되어 미생물로부터의 안정성은 더 장기간으로 실험해볼 필요가 있을 것으로 보인다. 또한 높은 항균 능력과 생분해능의 결과를 보여 저장 기간의 안정성이 높은 용기의 개발과 환경의 영향을 최소화 할 수 있을 것으로 판단된다. 따라서 돈피 젤라틴에 난각과 호두껍질 분말을 넣어 제조한 생분해성 용기의 개발의 기초 데이터로 이용될 수 있을 것으로 생각된다. This study analyzed the quality, microbial stability, and biodegradability of biodegradable containers manufactured using gelatin. Gelatin was extracted from beef, pork, and chicken skin. As a result of the gelatin experiment, the dried yield of pork gelatin was significantly higher than other treatments. It had the highest molecular weight in the SDS-PAGE, so it was selected as the primary material for this study. After mixing pork gelatin powder with water 1:3 (Pork Gelatin, PG), 20% eggshell, 10% walnut powder (Pork gelatin and Walnut 10%; PG-W1), and 20% (Pork gelatin and Walnut 20%; PG-W2) were mixed to prepare two treatments. As a result of the cross-sectional area, PGW1 showed fewer pores than PG-W2. PG-W1 showed significantly higher hardness than PG-W2. On the other hand, the compressive strength of PG-W2 was significantly higher. There is no expression in bacteria and E. coli during this period. PG-W1 showed high antibacterial ability compared to PG-W2. In addition, biodegradability also showed excellent results, based on this data. It is judged that more stable biodegradable containers can be developed.

Mechanical and Durability Characteristics of Latex-Modified Fiber-Reinforced Segment Concrete as a Function of Microsilica Content

Kim, Woong,Oh, Ri-On,Lee, Joo-Ha,Kim, Mi-Sol,Jeon, Sang-Min,Park, Chan-Gi Hindawi Limited 2019 Advances in civil engineering Vol.2019 No.-

<P>This study evaluated the performance of latex-modified fiber-reinforced concrete (RC) segments as a function of the substitution level of microsilica and type of reinforced fiber, to address the problem of corrosion of steel segments and steel-reinforced fiber segments, which are commonly used to shield tunnel-boring machine (TBM) tunnels in urban spaces. Our study compared macro synthetic, steel, and hybrid (macro synthetic fiber + polypropylene fiber) reinforcing fibers. The substitution levels of microsilica used were 0, 2, 4, and 6%. The target strengths were set at 40 and 60 MPa to test compressive strength, flexural strength, chloride ion penetration resistance, and impact resistance. Testing of latex-modified and fiber-reinforced segment concrete showed that the compressive strength, flexural strength, and chloride ion penetration resistance increased with an increasing substitution level of microsilica. These improvements were attributed to the densification of the concrete due to filling micropores with microsilica. Micro synthetic fiber was more effective in terms of improved compressive strength, flexural strength, and chloride ion penetration resistance than steel fiber. These results were due to the higher number of micro synthetic fibers per unit volume compared with steel fiber, which reduced the void volume and suppressed the development of internal cracks. The optimal microsilica content and fiber volume fraction of micro synthetic fiber were 6% and 1%, respectively. To evaluate the effects of the selected mixtures and hybrid fibers simultaneously, other mixing variables were fixed and a hybrid fiber mixture (combination of macro synthetic fibers and polypropylene fibers) was used. The hybrid fiber mixture produced better compressive strength, flexural strength, chloride ion penetration resistance, and impact resistance than the micro synthetic fibers.</P>

치유의 숲길 해설판이 이용객의 회복환경 및 전반적 치유효과 지각에 미치는 효과: 플라시보 효과의 적용^1a

김상미 ( Sang-mi Kim ),최솔아 ( Sol-ah Choi ),김상오 ( Sang-oh Kim ) 한국환경생태학회 2016 한국환경생태학회지 Vol.30 No.6

본 연구에서는 해설판의 `숲의 명칭` 또는 `숲의 치유 효과에 대한 정보`가 이용객의 회복환경지각(perceived environmental restorativeness: PER)과 전반적 치유효과지각(perceived healing effectiveness: PHE)에 미치는 영향을 조사하였다. 2015년 5-6월 중 연구대상지역인 무등산국립공원 내 `치유의 숲길` 방문객을 대상으로 편의표본추출 방법을 통해 247명의 표본이 선정되었으며 자기기입식 설문지기법을 통하여 데이터 수집이 이루어 졌다. 본 연구의 실험을 위해 `치유의 숲길`을 따라 설치한 해설판(숲의 명칭과 치유의 숲길의 치유효과에 관한 내용 포함)을 읽은 응답자는 플라시보그룹, 해설판을 읽지 않은 응답자는 컨트롤그룹으로 간주하였다. 분석결과, 컨트롤과 플라시보그룹 간 PER과 PHE는 전반적으로 차이가 없는 것으로 나타났다. 그러나 PER의 구성 요인별 비교 결과에 따르면 플라시보그룹이 `벗어남`(being away) 요인에서 컨트롤그룹에 비해 긍정적인 수치를 나타냈다. PER의 4개 요인(벗어남, 짜임새, 매혹감, 적합성) 모두 PHE에 영향을 미치는 것으로 조사되었으며(P<0.001) 이들 4개 요인은 PHE의 51.1%를 설명하였다. PHE에 미치는 영향력은 짜임새, 벗어남, 적합성, 매혹감 순으로 크게 나타났다. PER에 미치는 플라시보 효과는 남성,저연령층, 저 방문경험 그룹에서 나타났으며, PHE에 미치는 플라시보 효과는 남성, 소그룹, 혼자서 또는 친척/가족 동반그룹, 저 방문경험 그룹에서 발견되었다. 관리적 관점에서 몇 가지 시사점을 제시하였다. This study examined how `forest name` or `information of forest healing effectiveness` influence their perceived environmental restorativeness (PER) and perceived healing effectiveness (PHE). Study area was the `Forest Healing Road (FHR)` in Mudeungsan National Park. Data were collected from 247 visitors selected by convenient sampling method using questionnaire survey during May-June, 2015. Respondents who read interpretive signs (forest name and information on forest healing effectiveness of FHR were written) installed along the FHR were regarded as placebo group and respondents who didn`t read them as control group. The results showed that there were no overall differences on PER and PHE between control and placebo groups. Placebo group, however, rated more positive on `being away` factor of PER than control group. All four factors (i.e., being away, coherence, fascination, comparability) of PER statistically influenced PHE (p<0.001), and these factors explained 51.1% of PHE. The `coherence` was the most influential to PHE, followed by `being away`, `comparability`, and `fascination` in order. Placebo effects on PER were shown in male, in lower age group(age≤54), or respondents with lower visiting experience to FHR(≤20 times/year). Placebo effects on PHE were found in male, in small group (≤2 persons), in respondents who visited `alone` or `with relatives/family`, or in respondents with lower visiting experience to FHR(≤20 times/year). Some research and managerial implications were suggested.

야생동물의 존재에 대한 인지 및 야생동물의 종류(곰과 고라니)에 따른 국립공원 방문객의 휴양경험 비교

김상미 ( Sang Mi Kim ),최솔아 ( Sol Ah Choi ),김상오 ( Sang Oh Kim ) 한국환경생태학회 2015 한국환경생태학회지 Vol.29 No.4

This study examined the effects of wildlife or wildlife species on national park visitors`` perception of place attributes and recreational experiences. Data were collected from 502 users of Seongsamjae Rest area and Nogodan Shelter area in Jirisan National Park and 173 college students during May-June 2014 using survey questionnaire. Some simulated photographs of water deer and Asiatic black bears were used for the college student survey. Overall, awareness about wildlife inhabiting in Jirisan National Park (AW) was not related with one’s perception of place attributes (PPA) (i.e., crowdedness, naturalness, safety) and types of visitors`` recreational experiences. Respondents with higher awareness about the presence of Asiatic black bear (AABB), however, tended to perceive Jirisan National Park as a place that provides ``wild`` or ``natural`` recreational This study examined the effects of wildlife or wildlife species on national park visitors`` perception of place attributes and recreational experiences. Data were collected from 502 users of Seongsamjae Rest area and Nogodan Shelter area in Jirisan National Park and 173 college students during May-June 2014 using survey questionnaire. Some simulated photographs of water deer and Asiatic black bears were used for the college student survey. Overall, awareness about wildlife inhabiting in Jirisan National Park (AW) was not related with one’s perception of place attributes (PPA) (i.e., crowdedness, naturalness, safety) and types of visitors`` recreational experiences. Respondents with higher awareness about the presence of Asiatic black bear (AABB), however, tended to perceive Jirisan National Park as a place that provides ``wild`` or ``natural`` recreational opportunities compared to those with lower AABB. Differences in PPA (i.e., crowdedness, naturalness, safety) and types of recreational experiences were also found to be influenced by wildlife species. Respondents exposed to bear or water deer tended to perceive their recreational experiences as more ``wild``. Existence of wildlife in Jirisan National Park had a positive effect on the quality of visitors`` recreational experiences. Different wildlife species showed different levels of effectiveness to quality enhancement of recreational experience. Some practical implications of the study were discussed from a managerial point of view.

Sutterella faecalis sp. nov., isolated from human faeces

Byeong Seob Oh,JI-SUN KIM,유승엽,Seoung Woo Ryu,SEUNG-HWAN PARK,강세원,박잠언,Seung-Hyeon Choi,Kook-Il Han,Keun Chul Lee,Mi Kyung Eom,Min Kuk Suh,Han Sol Kim,Dong Ho Lee,Hyuk Yoon,김병용,이제희,Jung-SookLee,이주혁 한국미생물학회 2020 The journal of microbiology Vol.58 No.2

An obligately anaerobic, Gram-stain-negative, non-motile, non-spore-forming, and coccobacilli-shaped bacterial strain, designated KGMB03119T, was isolated from human faeces from a Korean. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate was a member of the genus Sutterella and most closely related to Sutterlla wadsworthensis KCTC 15691T (96.8% 16S rRNA gene sequence similarity). The DNA G + C content of strain KGMB03119T was 58.3 mol% as determined from its whole genome sequence. Strain KGMB03119T was asaccharolytic, catalase-positive, oxidase- and urease-negative. Furthermore, the isolate was positive for alkaline phosphatase, leucine arylamidase, acid phosphatase, arginine arylamidase, alanine arylamidase, and glycine arylamidase. The major cellular fatty acids (> 10%) of the isolate were C18:1ω9c and C16:0. Methylmenaquinone-5 (MMK-5, 100%) was the predominant isoprenoid quinone in the isolate. Based on the phylogenetic, physiological, and chemotaxonomic characteristics, strain KGMB03119T represents a novel species, for which the name Sutterella faecalis sp. nov. is proposed. The type strain is KGMB03119T (= KCTC 15823T = NBRC 114254T).

단핵구세포주 THP-1의 대식세포로의 분화 및 활성화에서 CO의 억제 효과

김다솔(Da Sol Kim),이미선(Mi Sun Lee),김한솔(Han Sol Kim),이혜윤(Hye Yun Lee),김오윤(Oh Yun Kim),강예린(Ye Rin Kang),손동현(Dong Hyun Sohn),김관회(Koanhoi Kim),박영철(Young Chul Park) 한국생명과학회 2017 생명과학회지 Vol.27 No.2

Carbon monoxide (CO)는 세포 보호의 기능을 가지는 항산화 효소인 heme oxygenase-1 (HO-1)의 대사산물로 세포성장, 아폽토시스, 염증에 대한 억제 효과를 보이는 것으로 보고가 이어지고 있고, 이에 관련된 연구가 활발히 진행되고 있는 실정이다. 본 연구에서는 CO가 단핵구의 대식세포로의 분화 및 그 활성화 과정에 미치는 영향을 인간 단핵구세포주 THP-1을 이용하여 조사하였다. CO-releasing compound인 CORM-2는 phorbol 12-myristate 13-acetate (PMA)로 자극한 THP-1 세포에서 viability와 증식에는 큰 영향을 주지 않았으나 부착능의 뚜렷한 감소를 보였다. 그리고, CORM-2는 대식세포의 막표면 분화 인자인 CD14, CD11b 및 CD18의 발현과 latex beads를 이용한 포식 기능을 현저히 억제하였다. 다음으로, 배양중인 THP-1 세포를 PMA로 6일 동안 대식세포로 분화시킨 후 inflammatory cytokines의 분비와 포식 기능을 조사하였다. CORM-2의 처리는 lipopolysaccaride (LPS)로 자극한 대식세포로부터 분비되는 IL-6와 TNF-α의 분비를 감소시켰다. 또한, 분화된 대식세포에 E. coli (K-12 strain) bioparticles를 이용하여 포식 기능을 측정한 결과 CORM-2를 처리한 세포에서는 현저히 감소되는 경향을 보였다. 이를 종합해 볼 때, CO는 항원 인식과 포식 기능에 관여하는 막단백질의 발현을 저해함으로써 단핵구의 분화과정을 억제하였고, 분화된 대식세포의 inflammatory cytokines의 분비 및 포식 기능을 저해함으로써 활성화 과정도 억제하는 것으로 보인다. Carbon monoxide (CO), a reaction product of cytoprotective enzyme heme oxygenase-1 (HO-1), is a gaseous messenger with anti-proliferative, anti-apoptotic, and anti-inflammatory actions in many cell types. Here, we investigated the role of CO on the process of monocyte differentiation induced by phorbol 12-myristate 13-acetate (PMA) in human monocytic THP-1 cells. CORM-2 (tricarbonyldichlororuthenium (II) dimer, Ru₂Cl₄ (CO)6), a CO-releasing compound, decreased a marked cell adherence with a slight reduction of proliferation in monocytic THP-1 cells treated with PMA. And, CORM-2 significantly inhibited expression of differentiation markers such as CD14, CD11b plus CD18 (macrophage-1 antigen, Mac-1 or complement receptor 3, CR3) and phagocytosis of carboxylate-modified red fluorescent latex beads, in PMA-stimulated THP-1 cells. For the further experiments, differentiation of PMA-treated cells was enhanced after the initial 2 days stimulus by removing the PMA-containing media then incubating the cells in fresh media for a another 4 days. And, we observed the secretion of inflammatory cytokines and phagocytosis in differentiated macrophages. Treatment with CORM-2 significantly abolished the secretion of IL-6, TNF-α and phagocytosis using fluorescence-conjugated E. coli (K-12 strain) bioparticles in lipopolysaccharide (LPS)-stimulated differentiated macrophages. In conclusion, these results suggest that CO inhibits the differentiation of monocytic THP-1 cells as well as the activation of differentiated macrophages.

정상폐세포에서의 가습기살균제 in vitro 독성평가

박창위(Chang-We Park),박솔(Sol Park),김미지(Mi-Jie Kim),김하룡(Ha-Ryong Kim),오승민(Seung-Min Oh),정규혁(Kyu-Hyuck Chung) 한국환경독성학회 2012 한국독성학회 심포지움 및 학술발표회 Vol.2012 No.5