Forced swimming stress increases natatory activity of lead-exposed mice

Araujo Ulisses C.,Krahe Thomas E.,Ribeiro-Carvalho Anderson,Gomes Regina A. A.,Lotufo Bruna M.,Moreira Maria de Fátima R.,de Abreu-Villaça Yael,Manhães Alex C.,Filgueiras Cláudio C. 한국독성학회 2021 Toxicological Research Vol.37 No.1

Recent evidence points to the relationship between lead toxicity and the function of the hypothalamic-pituitary-adrenal axis, which suggests that lead exposure could influence how an individual cope with stress. Here we test this hypothesis by investigating the behavioral effects of lead exposure in mice during the forced swimming test (FST), a parading in which animals are exposed to a stressful situation and environment. Swiss mice received either 180 ppm or 540 ppm of lead acetate (Pb) in their ad-lib water supply for 60–90 days, starting at postnatal day 30. Control (Ctrl) mice drank tap water. At the end of the exposure period, mice were submitted to a 5-min session of FST or to an open-field session of the same duration. Data from naïve animals showed that corticosterone levels were higher for animals tested in the FST compared to animals tested in the open-field. Blood-lead levels (BLL) in Pb-exposed mice ranged from 14.3 to 106.9 μg/dL. No differences were observed in spontaneous locomotion between Ctrl and Pb-exposed groups in the open-field. However, in the FST, Pb-treated mice displayed higher swimming activity than Ctrl ones and this effect was observed even for animals with BLL higher than 20 μg/ dL. Furthermore, significant differences in brain glutathione levels, used as an indicator of led toxicity, were only observed for BLL higher than 40 μg/dL. Overall, these findings suggest that swimming activity in the FST is a good indicator of lead toxicity and confirm our prediction that lead toxicity influences behavioral responses associated to stress.

Field and Laboratory Investigation of Cold In-Place Recycling in Vietnam

Manh Tuan Nguyen,Ba Khanh Le,Manh Cuong Nguyen 한국도로학회 2018 한국도로학회 학술대회 발표논문 초록집 Vol.2018 No.05

In Vietnam, reclaimed asphalt pavement technology is considered as the key to reduce the cost of pavement construction, and decrease the industrial waste from flexible pavement rehabilitation. Ministry of Transportation has concentrated to develop RAP technology since 2008. As a result, there are three trademark of recycling technologies applied in Vietnam such as Wirtgen (Germany), Hall Brothers (U.S.), and Sakai (Japan). This paper is focused on asphalt pavement investigation of field and laboratory tests from Cold In-place recycling technology. The laboratory tests carried out on the material were conventional tests including Marshall stability, indirect tensile strength... Field data was collected using a Benkelman beam and core specimens for indirect tensile strength test.

Cytotoxic and apoptosis-inducing activities against human lung cancer cell lines of cassaine diterpenoids from the bark of <i>Erythrophleum fordii</i>

Ha, Manh Tuan,Tran, Manh Hung,Phuong, Thien Thuong,Kim, Jeong Ah,Woo, Mi Hee,Choi, Jae Sue,Lee, Suhyun,Lee, Jeong Hyung,Lee, Hyeong Kyu,Min, Byung Sun Elsevier 2017 Bioorganic & medicinal chemistry letters Vol.27 No.13

<P><B>Abstract</B></P> <P>A phytochemical investigation into the bark of <I>Erythrophleum fordii</I> yielded four new compounds, two new cassaine diterpenoids (erythrofordin T and U, <B>1</B> and <B>2</B>) and two new cassaine diterpenoid amines (erythroformine A and B, <B>6</B> and <B>7</B>), as well as nine known compounds. We report for the first time the isolation of erythrofordin V (<B>3</B>) from a natural source and that of the remaining eight known diterpenoids (<B>4</B>–<B>5</B>, <B>8</B>–<B>13</B>) from <I>E. fordii</I>. All structures were elucidated using spectroscopic analysis. Cytotoxic activity of the isolated compounds (<B>1</B>–<B>13</B>) was examined <I>in vitro</I> against three non-small cell lung cancer cell lines (A549, NCI-H1975, and NCI-H1229) using the MTT assay. Cassaine diterpene amines (<B>6</B>–<B>10</B>, <B>12</B>, <B>13</B>) exhibited potent cytotoxic activity against all three cell lines with IC<SUB>50</SUB> values between 0.4μM and 5.9μM. Erythroformine B (<B>7</B>) significantly induced apoptosis in all three cancer cells in a concentration-dependent manner.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

α-Amylase and Protein Tyrosine Phosphatase 1B Inhibitoryof Some Vietnamese Medicinal Plants Used to Treat Diabetes

Tran Manh Hung,Hoang Duc Manh,Pham Thi Hong Minh,윤의중,나민균,Won Keun Oh,민병선,배기환 한국생약학회 2007 Natural Product Sciences Vol.13 No.4

this study, the twenty-four ethyl acetate extracts of twenty-two medicinal plants, traditionally usedα-amylase and protein tyrosine phosphatase 1B (PTP1B)enzymes inhibitory activity in vitro. The results indicated that, twelve materials (50.0%) showed moderate tostrong inhibitory activity in α-amylase inhibitory activity with IC50 values ranging from 2.5 to 48.8g/mL;meanwhile, ten extracts (41.6%) could demonstrate PTP1B activity with IC50 values less than 30.5g/mL. Someplants presented interesting activities against both of α-amylase and PTP1B enzymes such as Catharanthusroseus, Carthamus tinctorius, Momordica charantia, Gynostemma pentaphyllum, Glycyrrhiza glabra, Smilaxglabra, Psidium guajava (leave), and Rehmannia glutinosa. The study may provide a proof, at least in a part, forthe ethno-medical use in diabetes disease of these plants. KeywordsVietnamese medicinal plants, diabetes, α-amylase, protein tyrosine phosphatase 1B

Adaptive Control for Uncertain Model of Omni-directional Mobile Robot Based on Radial Basis Function Neural Network

Duyen Ha Thi Kim,Tien Ngo Manh,Cuong Nguyen Manh,Nhan Duc Nguyen,Dung Pham Tien,Manh Tran Van,Minh Phan Xuan 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.4

The paper proposes the method to deal with control problems of unmodeled components of the fourwheeled Omni-directional mobile robot. It is commonly challenging to design a model-based control scheme to achieve smooth movement in the tracking process due to the unknown elements in the mathematical model of the robot or external disturbances. Our main contribution focuses on designing an adaptive controller based on neural networks with online weight updating laws and Fuzzy logic to guarantee the high accuracy of the robot’s movement when the unknown factors adversely affect the robot control. At the initial step, a Dynamic Surface Control plays a role as a core of the controller for the robot system. Then, with the ability to estimate the appropriate value for uncertain nonlinear parts, a Radial Basis Function Neural Network is designed. Finally, a Fuzzy law is to utilize control parameters in each period to increase the adaptive behavior of the system. The stability and convergence of the system are proven by the Lyapunov’s stability theory. The simulation results illustrate the validity and the efficiency of the proposed control algorithm when the system is lack of robot model’s information.

A Motion Control System Developed for Research and Education Purposes

Manh-Tuan Ha,Chul-Goo Kang 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10

Research and education on motion control requires an easy-to-use and economic experimental apparatus, but present commercial products are expensive, complex in mechanism and hard to use them in general. In this paper, we present an interesting motion control apparatus for research and education which is easy-to-use and economic. For user flexibility, we use an open-source free operation system Linux and a free real-time kernel RTAI. The developed motion control system is reliable in mechanism, and fast enough to implement 100 microsecond sampling time for real-time control. The developed motion control system has been demonstrated by testing PD (proportional-plus-derivative) control and ZV (zero vibration) input shaping experimentally.

Elastic structure for a multi-axis forcetorque sensor

Manh-Tuan Ha,강철구 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.1

The multi-axis force–torque sensor is a key element of many accurate force and motion control systems, including intelligent robotic systems. This paper presents a novel elastic structure for multi-axis force–torque sensors that is easily manufacturable at low cost and has many detection points with high surface strains. By using the “design of experiment” method in the ANSYS software, we demonstrate that the designed elastic structure with four identical T-shapes has high surface strains and can provide various strain gauge configurations. The strain sensitivities of the elastic structure at predetermined points can be maximized manually by varying the thickness of the vertical and horizontal beams of the T-shaped components. The validity of the proposed elastic structure is demonstrated through the development of a low-cost six-axis force–torque sensor. Experimental results for the developed force–torque sensor with the proposed elastic structure demonstrate that the proposed structure is comparable to an expensive state-of-the-art commercial force–torque sensor.

Enhanced Cathodoluminescence of KOH-treated InGaN/GaN LEDs with Deep Nano-Hole Arrays

Manh-Ha Doan,이재진 한국광학회 2014 Current Optics and Photonics Vol.18 No.3

Square lattice nano-hole arrays with diameters and periodicities of 200 and 500 nm, respectively, are fabricated on InGaN/GaN blue light emitting diodes (LEDs) using electron-beam lithography and inductively coupled plasma reactive ion etching processes. Cathodoluminescence (CL) investigations show that light emission intensity from the LEDs with the nano-hole arrays is enhanced compared to that from the planar sample. The CL intensity enhancement factor decreases when the nano-holes penetrate into the multiple quantum wells (MQWs) due to the plasma-induced damage and the residues. Wet chemical treatment using KOH solution is found to be an effective method for light extraction from the nano-patterned LEDs, especially, when the nano-holes penetrate into the MQWs. About 4-fold CL intensity enhancement factor is achieved by the KOH treatments after the dry etching for the sample with a 250-nm deep nano-hole array.

Spousal concordance of ideal cardiovascular health metrics: findings from the 2014–2019 Korea National Health and Nutrition Examination Survey

Manh Thang Hoang,Lee Hokyou,김현창 대한고혈압학회 2022 Clinical Hypertension Vol.28 No.-

We aimed to investigate whether a spouse’s cardiovascular health (CVH) metrics status affects the other spouse’s ideal CVH using a Korea nationwide representative survey.We used the health data of 6,030 married couples who participated in the Korea National Health and Nutrition Examination Survey from 2014 to 2019. The CVH was defined using seven metrics: smoking status, blood pressure, body mass index, total cholesterol, fasting blood glucose, physical activity, and diet, following the American Heart Association guidelines and modifications for body mass index cutoffs and diet quality. The CVH score was calculated on a scale ranging from 0 to 7, with the ideal CVH defined as attaining ideal scores in at least five CVH metrics. Multiple logistic regression analyses were used to assess whether husband’s ideal CVH was associated with his wife’s odds for having ideal CVH, and vice versa.The mean CVH scores were 3.2 and 4.0 for husband and wife, respectively. After fully adjusting for age and education of both partners and household income, husbands had 1.49 times (95% confidence interval [CI], 1.27–1.69) higher odds of achieving ideal CVH if their wives had also achieved ideal CVH. Meanwhile, wives whose husbands achieved ideal CVH also had 1.46 times (95% CI, 1.27–1.69) higher odds of achieving ideal CVH. Nonsmoking (57.17%), ideal fasting blood glucose level (34.93%), and ideal diet intake (24.18%) were the most concordant CVH metrics among spouses.Our study found a significant spousal concordance of ideal CVH in Korean married couples. This finding supports the use of a couple-based interventional strategy targeted to promote CVH.

The Mixture of Glycitin and TDB (3-(2,4,6-trimethoxyphenyl)-2,3- dihydro-1H-benzo[f]chromen-1-one) Could Ameliorate Skin Ageing by Anti-wrinkle and Anti-melanogenesis Effects in Dermal Fibroblasts and Melanocytes

Manh Tin Ho,고동수,조문재 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.6

Ultraviolet (UV) radiation in sunlight can induce skinageing and photo-carcinogenesis. UV may also induce melaninproduction and wrinkle formation. Recently, natural secondarycompounds have been reported to have protective effects againstUV light. In this study, the effects of two different compounds,glycitin and 3-(2,4,6-trimethoxyphenyl)-2,3-dihydro-1H-benzo[f]chromen-1-one (TDB), on human dermal fibroblasts andmelanocytes were investigated. At first, only TDB was used onmelanocyte cells to test whether it inhibited the proliferation ofthese cells. Then, a mixture of glycitin and TDB was tested onhuman dermal fibroblasts for 48 h in order to investigate its effecton proliferation, collagen production, and metalloproteinase familyexpression. The TDB treatment alone not only inhibited theproliferation of melanocytes but also increased extra cellularmatrix production in dermal fibroblasts and cell viability. Themixture of glycitin and TDB markedly increased fibroblastproliferation and helped to maintain fibroblast viability in the faceof UV-induced and H2O2-induced damages. This co-treatmentalso significantly promoted collagen IV expression and acceleratedtotal collagen secretion. In addition, the metalloproteinase (MMPs)family such as MMP1, MMP2, and MMP7 were down-regulatedat the transcriptional level. In conclusion, the mixture of glycitinand TDB induced fibroblast proliferation even when thesefibroblasts were damaged by UV exposure and H2O2, whereasaugmented collagen production and the inhibition of MMPsreduced wrinkle formation and decreased melanocyte proliferation,suggesting a potential use in UV-protective therapy.