알코올 투여로 온 흰쥐의 혈액성분의 변화와 간조직 손상과의 상호관계에 관한 연구

조만희,김창세 순천향대학교 1986 논문집 Vol.9 No.2

The white rats were divided by 3 groups; 0.6g(group A), 1.8g(group B) and 4.8g(group C) of alcohol per kg of body weight. The ethanol has been administered to the stomach with a polyethylene catheter using 20% ethanol solution for 30 days. In 5 day interval from the begining, the white rats were sacrificed to collect sample for blood analysis and histopathological observation. And the following results were obtained. 1. The most variable changes of blood components to be influenced by alcohol administration were; alcohol concentration, activities of liver ADH and GOT, numbers of RBC and WBC, amounts of triglyceride and total cholesterol and α₂-globulin fraction. 2. The liver histopathological changes, depending on the amounts and feeding days from the alcohol administration, were observed; normal finding on 1st to 10th and after then mild ballooning degeneration, necrosis of hepatic parenchymal cells in portal tract, mild infiltration of lymphocytes and fats degeneration. 3. The interrelationship between changes of blood components and histopathological damage of liver were coincide on 10th to 20th in the aspects of remarkable changes. From the above summary the amount and duration of ethanol-administration were influenced on changes of blood components and histopathological findings of liver while the early period usually 1st to 10th day was observed no more pathological changes but after then significants changes observed. From the above result there were suggested that the alcohol retaining in the body in a long period were results to appear in biochemical metabolic change.

Carotenoids 식이와 알코올이 혈액학적 성분과 간조직에 미치는 영향에 관한 연구

조만희,김연선,이상한,우기민,장예진,김창세 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.2

Alcohol is well known agent which can damage the human tissues such as liver via stimulating lipid peroxidation and storage, denaturation of macromolecules, and inhibiting protein metabolism. On the other hand, carotenoids in addition to vitamins A, C, E and lipoic acid, play important roles in protecting these oxidative damages as well as preventing the production of free radicals. This study was carried out to elucidate the precise effects of alcohol administration into rats on the antioxidative functions of dietary carotenoids and isolated β-carotene, and to find out any parameters to uncover more detailed biochemical mechanisms of these agents. For these purposes, two different approaches were performed: 1) examination of the changes in hematological parameters (e.g., total proteins, A/G ratio, ALT/AST et. al) and statistical correlations among inter- and intragroups, 2) examination of the histopathological changes by an electron microscope. The results were analyzed and summarized as following; (1) Carrot diet for one week caused a slight increase in albumin. A/G ratio and AST levels, and a slight decrease in ALT, ALP, BUN and uric acid levels. Two-week alcohol administration following carrot diet increased total proteins, albumin A/G ratio and BUN levels. However, both groups were shown to have little significant changes in cholesterol concentrations. (2) Cheese diet for one week caused a significant increase in total proteins, albumin, AST, ALP, uric acid and total cholesterol levels, and a significant decrease in A/G ratio and glucose concentrations. However, two-week β-carotene diet following cheese intake increased total proteins albumin, A/G ratio, BUN and total cholesterol levels, whereas AST, ALT, ALP and uric acid levels were decreased. (3) Alcohol administration for one week caused an increase in AST activities and a decrease in total proteins, albumin, ALT, ALP and BUN levels. A successive β-carotene diet following alcohol administration increased total proteins, albumin, ALT, ALP and BUN levels, whereas decreased AST and uric acid levels. However, there were not significant changes in A/G ratio, glucose and total cholesterol levels in both groups. (4) β-carotene diet for one week caused a slight increase in albumin, glucose, AST and uric acid levels, and a slight decrease in ALP and BUN levels. Two-weeks alcohol administration following β-carotene diet increased albumin, glucose, BUN and total cholesterol levels, whereas decreased AST, ALT, ALP and uric acid levels. (5) Although the pathological investigation on the liver did not reveal significant changes, cheese diet group (CH-BC/1-CH) was shown to have some lipid deposits. Some results were unexpected and different from typical hematological changes shown by other researchers. Nonetheless, these results strongly suggest that the liver damage or hepatism caused by alcohol intake affects many kinds of biochemical metabolisms, which results in significant changes in many hematological parameters. In addition, dietary carotenoid and isolated β-carotene were shown to have protective roles against the biochemical changes by alcohol intake.

알코올 및 인삼액이 효소활성도에 미치는 영향에 관한 연구

신경현,조만희,김창세 순천향대학교 1994 논문집 Vol.17 No.2

This study was investigated for the change of r-GTP, CPK activity and CPK-isoenzyme by alcohol and Ginseng administration, The normal white S.D rats were classified with four groups; ethanol treated was group A, ethanol pretreated group combined pretreated with Ginseng extract was group B, Ginseng extract treated was group C, and Ginseng extract treated group combined pretreated with ethanol was group D. The white rats of four groups were treated with 20%, 30%, 40% ethanol and/or 1% Ginseng extract. Then enzyme activities such as r-GTP, CPK and CPK-isoenzyme had been measared in serum, hepatic, and renal tissues. The sample was collected by 5 day intervalus for 25 days. The following results were obtained. 1. The changes of serum r-GTP activities were significantly increased in ethanol-treated group, decreased in Ginseng-treated group. 2. The changes of hepatic r-GTP activites were significantly increased in ethanol-treated group, decreased in Ginseng-treated group. 3. The changes of renal r-GTP activites were significantly increased in ethanol-treated group, decreased in ginseng-treated group. 4. The changes of serum CPK activites were significantly increased in ethanol-treated group, decreased in ginseng-treated group. 5. The changes of hepatic and renal tissue CPK activites were not significant. 6. The changes of serum and hepatic tissue CPK-isoengyme were significantly increased in ethanol-treated group, decreased in ginseng-treated group combined pretreated with ethanol. 7. The changes of CPK-isoengyme, CPK 1 and CPK 2 were not significant.

Colchicine 독성에 따른 골격근의 형태학적 변화에 관한 연구 : 막성소체 출현에 관한 전자현미경적 검색 Electron Microscopic Study

이화동,김경수,장희경,허만하 대한신경외과학회 1995 Journal of Korean neurosurgical society Vol.24 No.11

To investigate the morphological changes in skeletal muscle produced by colchicine toxicity, 42 rats were given a single intraperitoneal dose(0.4mg/kg) of colchicine, and the animals were killed at daily intervals up to 7 days. The results of light microscopic and electron microscopic observations are summarized as follows : 1) At the light microscopic level, colchicine treated rats show non-inflammatory, nonspecific scattered degeneration or necrosis of myofibers irrrspective of location of the skeletal muscle. No significant pathologic changes in peripheral nerve fibers distributed in affected muscles are seen.2) The general ultrastructural changes are dilation of sarcoplasmic reticulum, swelling and pleomorphism of mitochondria, and appearance of membraneous bodies characterized by a single or multiple concentric layers of membranes in subsarcolemmal zones and intermyosbrillar zones, and focal necrosis or loss of myofibrils. The incidence and severity of these changes show gradual increase and reach maximal peak 3 days after colchicine administration, and then they show gradual decrease. Nerve fibers and motor-end plates show no difference compared with the control. 3) The observations are consistent with the opinion that the muscle weakness or paresis produced by toxic dose of colchicine is not of neuropathic, but of myopathic alterations. 4) The membraneous bodies are classified into the following 3 types : Type Ⅰ is those bodies outlined by a few concentric membranes with osmiophilic granules, small vesicles, free ribosomes, and mitochondria in central zone. Type Ⅱ is those outlined by several concentric layers of membranes with osmiophilic gradules, small vesicles, free ribosomes, and mitochondria in center. Type Ⅲ is those outlined by multilayered or thick concentric membranes with osmiophilic granules, small vesicles, free ribosomes, and mitochondria in central zone, frequently with complicated lamellar structures. 5) The skeletal muscle lesions produred by colchicine toxicity may be categorized into those degenerative changes characterized by the appearance of membraneous bodies. And, morphologically, it is presumed that the membraneous bodies are heterogenous in origin, alterations of subcellular structures such as sarcoplasmic reticulum, mitochondria, and lysosome.

개에서 동소성 간이식술시의 대사 및 혈역학적 변화

이창준,홍순용,신근만,최영룡,이영주,문종선 대한마취과학회 1993 Korean Journal of Anesthesiology Vol.26 No.3

Skilled and experienced anestheia is of great importance for patients undergoing orthotopic liver transplantation, because of multiple preexisting medical problems in such patients as well as the intraoperative problems of rapid hemodynamic, metabolic, and coagulation changes. In this study, the intraoperative hemodynamic and laboratory data were analyzed in ten dogs that underwent an orthotopic liver transplantation procedure by veno-venous bypass using Biopump. Liver transplantation can be divided into three distinct periods: stage I, or preanhepatic stage, which begins with the induction of anesthesia and continues until cross clamping of portal vein and IVC; stage II, or anhepatic stage, which begins at the anhepatic time and continues until the donor liver is reperfused by the recipients circulating blood; and stage III, or postanhepatic stage, which begins at the time of reperfusion and continues until the end of surgical procedure. The hemodynamic changes at the time of IVC and portal vein cross clamping were decreases in CVP, PCWP, and pulmonary artery pressure in spite of using Biopump. The significant metabolic alternations during anhepatic stage were decrease in blood glucose levels and increase in blood lactate levels. The more significant hemodynamic changes occurred at the time of reperfusion. Systolic pressure decreased suddenly to 58±6 mmHg and cardiac output decreased to 1.08±0.1l L/min. However heart rate, pulmonary artery pressure, CVP, and PCWP did not change significantly. During stage III, hyperglycemia occurred quite frequently. Significant abnormal coagulation chages could not be found, probably because the dogs were healthy. In conclusion, during anhepatic stage, we have to compensate for alternations of fluid balance. At the time of reperfusion, we should prevent severe hemodynamic changes and treat them immediately if they occur. However, it seems that glucose administration is not necessary to the liver recipient during stage II because there is no significant hemodynamic depression e to hypoglycemia at this time and hyperglycemia occurs later

알코홀이 r-GTP 활성에 미치는 인삼의 영향

이상한,조만희,신경현,김창세 순천향대학교 1989 논문집 Vol.12 No.3

This study was carried out to investigate the effects on the changes of the r-glutamyl transpeptidase activity of ethanol-administered white rats. There was classified into group; ethanol treated group(group A), ethanol treated group which was pretreated with ginseng extract(group B), ginseng extract treated group(group C), and ginseng extract treated group which was pretreated with ethanol(group D). White rats of four groups were treated with combined 1% ginseng extract and / or 20% ethanol for 30 days, and were measured the changes of r-GTP activity on the serum, hepatic and renal tissue sample collected at 5 days interval. Following results have been obtained. 1. The changes of serum r-GTP activity were significantly decreased in ethanol treated group B which was pretreated with ginseng extract than in ethanol treated group A. The values were highly significant at the 20th day of feeding. 2. The changes of hepatic r-GTP activity were more increased in group A and decreased in group D. The values were found significantly lowest in 10th and highest in 5th days. 3. The changes of renal r-GTP activity were more increased in group A and decreased in group D. The values were significantly highest in 10th and lowest in 20th days. Based on these results it was found that the changes of r-GTP activity between serum and hepato-renal tissue were exist and the protective action of ginseng against alcohol degradative effects was noted.

한국·미국·스페인 청소년의 태권도 수련이 인성교육에 미치는 영향

유창재,양정옥,이중숙,이상돈,김영수,조만태 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.6

The purpose of this study was to analyzed what effect Taekwondo training has upon Juvenile trainees in Korea, America, and Spain. to see if there is a significant difference in their Human nature Education. The subject of this study was set up as 547 Juvenile trainees who are including 183 Koreans, 185 Americans and 179 Spanish people belonging to gymnasia in their own countries, and sampling by purpose sampling. The means in this study is used to amend and complemented after confirming suitability of the contents in the questionnaire that is made on the basis of questionnaire Lee Chul Ho(1996). it is used as study means after modified, whether the substance of question is right in fitness and applicability or not, Reliability of the question, the range of Cronbach's a is showed from .7924 to .8450. The method of statistically analysis adapted one-way ANOVA, two-way ANOVA by using SPSS WIN 10.0 Program. The result of this study are as followed : First, it can be seen that American Juvenile and Spanish Juvenile show more significant change than Korean Juvenile in their Human nature Education by the Taekwondo training according to their nationality. Second, it can be seen that Juvenile who have had the Taekwondo training for more than from three to five year show more significant change in their emotion, sociability and attitude towards life than those who have had the same training for more than other period in their Human nature Education according to their Taekwondo training period. Third, as a result of analytic investigation of what effect the same training has upon the change factors in their Human nature Education in accordance with their training period and nationality, it can be seen that the training itself and nationality has the greater influence on them than the training period. Therefore, judging from the above findings, it can be proved that the Taekwondo training has much more significant and positive effect on Juvenile in their change of emotion, sociability and attitude towards life.

통합 차량 네트워크 시스템을 위한 노드 기반 FlexRay-CAN 게이트웨이 설계

김만호(Man Ho Kim),이석(Suk Lee),이경창(Kyung Chang Lee),하경남(Kyong Nam Ha) 한국자동차공학회 2011 한국자동차공학회 부문종합 학술대회 Vol.2011 No.5

As vehicles become more intelligent, in-vehicle networking (IVN) systems such as controller area network (CAN) or FlexRay are essential for the convenience and safety of drivers. To expand the applicability of IVN systems, attention is currently being focused on the communication between heterogeneous networks such as body networking systems or chassis networking systems. Message mapping based gateway was developed to communicate between FlexRay and CAN network to improve exchanging information of vehicle. However, there are obstacles for the wide acceptance of the FlexRay-CAN gateway of vehicle. First, when message ID was changed of network, gateway must be reloaded changed message mapping table. Second, if the exchanging message is increased in the network, software complexity of gateway is increased rapidly. In order to overcome these obstacles, this paper presents a node mapping -based FlexRay-CAN gateway for IVN systems. In addition, this paper presents a node mapping based FlexRay-CAN gateway operation algorithm along with the experimental evaluation for ID change.

Development of Controlled Release Oral Drug Delivery System by Membrane-Coating Method-I - Preparation and pharmaceutical evaluation of controlled release acetaminophen tablets-

Shim, Chang-Koo,Kim, Ki-Man,Kim, Young-Il,Kim, Chong-Kook The Pharmaceutical Society of Korea 1990 Archives of Pharmacal Research Vol.13 No.2

In order to develop a controlled-release oral drug delivery system (DDS) which sustains the plasma acetaminophen (AAP) concentration for a certain period of time, microporous membrane-coated tablets were prepared and evaluated in vitro. Firstly, highly water-soluble core tablet of AAP were prepared with various formulations by wet granulation and compression technique. Then the core tablets were coated with polyvinychloride (PVC) in which micronized sucrose particles were dispersed. Effect of formula compositions of core tablets and coating suspensions on the pharmaceutical characteristics such as drug release kinetics and membrane stability of the coated tablets was investigated in vitro. AAP was released from the coated tablets as a zero-order rate in a pH-independent manner. This independency of AAP release to pH change from 1.2 to 7.2 is favorable for the controlled oral drug delivery, since it will produce a constant drug release in the stomach and intestine regardless of the pH change in the GI tract. Drug release could be extended upto 10 h according to the coating condition. The release rate could be controlled by changing the formula compositions of the core tablets and coating suspensions, coat weight per each tablet, and especially PVC/sucrose ratio and particle size of the sucrose in the coating suspension. The coated tablets prepared in this study had a fairly good pharmaceutical characteristics in vitro, however, overall evaluation of the coated tablet should await in vivo absorption study in man.

ICAO 국제항공안전정책 패러다임의 변화 분석과 우리나라 신국제항공안전정책 검토

장만희 ( Man Heui Chang ),황호원 ( Ho Won Hwang ) 한국항공우주법학회 2013 한국항공우주정책·법학회지 Vol.28 No.1

ICAO는 기존의 항공안전평가제도를 개선한 항공안전평가제도(USOAP)를 1995년 도입하였다. 이는 최근의 항공기 사고율 감소에 효과적인 역할을 하고 있다고 평가되고 있으며 이러한 성과를 바탕으로 2013년부터는 기존의 ‘스냅샷 방식’에서 ‘상시 모니터링 방식(USOAP-Continuous Monitoring Approach)’으로 전환하였다. ICAO 항공안전평가 결과는 오늘날 국제사회에서 국가의 ‘항공안전 신인도’를 판단하는 객관적인 지표로써 항공산업에 막대한 영향을 미치고 있으며, 특히 평가결과가 미흡할 경우엔 항공노선 확충 및 코드쉐어 금지, 환승객 감소 국제항공 비즈니스 및 보험료 인상 등 경제적 불이익을 감수해야 한다. 또한, ICAO는 기존의 법규이행(Prescriptive Approach)을 기본으로 하되 리스크 기반(Risk-based) 사전예방형(Proactive Approach) 항공안전시스템 개념을 새로 도입하는 등 국제항공안전정책 패러다임을 전환하여 왔다. ICAO가 새로운 국제항공안전정책을 추진함에 따라 대한민국 정부도 이에 발맞춰 국내항공안전정책을 변화시켜 왔다. 특히, 시스템적 안전관리를 위해 기존의 법규이행 중심의 정부 안전감독시스템에 리스크 기반의 사전 예방적 안전관리 개념을 도입하고 있으며, ICAO 국제기준에 따라 항공서비스제공자에게 자체 안전관리시스템(SMS)를 운영토록 항공법에 규정하였다. 또한, 항공안전의 중심 분야인 항공기 운항 및 정비 분야에 대한 안전 증진을 위해 다양한 안전정책을 추진하고 있다. 이러한 신 국제항공안전정책 패러다임 변화에 발맞춰 국제사회에서 가장 모범적인 항공안전시스템을 갖춘 나라로 확고히 자리 매김하고, 우리 항공의 경쟁력을 튼튼하게 뒷받침한다는 계획이다. 이를 위해서는 정부, 항공사, 연구기관 등 항공관계자가 모두가 노력할 때 실질적인 효과를 기대할 수 있을 것이다. ICAO`s Universal Safety Oversight Audit Programme (USOAP) was initially launched in January 1995, in response to widespread concerns about the adequacy of aviation safety oversight around the world. The recent reduction in aircraft accidents and effective role that is evaluated on the basis of these results, and in 2013 the existing ‘snapshot approach`` to ‘regular monitoring system (USOAP-Continuous Monitoring Approach)`` was converted to. ICAO aviation safety assessment of the state in today`s international community ‘aviation safety credibility`` as objective indicators to judge the enormous impact on the aviation industry, the state is not satisfactory, especially if the results of the evaluation and expansion of code-share airline ban, reduced international air transit passengers, including premium increases business and economic penalties should. In addition, ICAO implementation of the existing laws and regulations(Prescriptive Approach), but based on the Risk-based prevention model, Proactive Approach introduced the concept of aviation safety system, including international aviation safety policy has been to switch paradigms. This new ICAO international aviation safety policy also applies to the Government of the Republic of Korea in line with the aviation safey policies have changed. In particular, the systematic implementation of safety management for the existing laws and regulations in the center of the safety oversight system of risk-based introduction of the concept of proactive safety management, and According to international standards ICAO aviation service providers operate their own Safety Management System was set out in Aviation Law ever. In addition, the aviation safety is at the center of the field of the safety of aircraft operations and maintenance for the promotion is promoting various safety policies. This new paradigm shift in the international aviation safety policy in line with our state in the international community with the most exemplary aviation safety system firmly established itself as a model, the Government will strengthen the competitiveness of our aviation plans to support. To do this, the government, airlines, aviation officials try all the practical effect would be expected.