Electrospun Poly(ε-caprolactone)/Gelatin Nanofibrous Mat Containing Selenium as a Potential Wound Dressing Material: In Vitro and In Vivo Study

Majid Salehi,Keyvan Shahporzadeh,Arian Ehterami,Hodays Yeganehfard,Heliya Ziaei,Mohammad Moein Azizi,Saeed Farzamfar,Amirreza Tahersoltani,Arash Goodarzi,Jafar Ai,Akbar Ahmadi 한국섬유공학회 2020 Fibers and polymers Vol.21 No.8

In the current study, selenium particles were loaded into poly(ε-caprolactone)/gelatin nanofibrous matrices in orderto fabricate a potential wound dressing. The mats were produced by electrospinning of poly(ε-caprolactone)/Gelatin (1:1(w/w)) solution supplemented with 1 and 10 % (w/v) of selenium particles. Prepared wound dressings were investigatedregarding their morphology, mechanical properties, surface wettability, water-uptake capacity, water vapor permeability,porosity, blood compatibility, microbial penetration test and cellular response. Dressings containing 1 % and 10 % seleniumand selenium free mats were chosen to treat the full-thickness excisional wounds in Wistar rats. The study revealed that after14 days, the wound closure of the mat containing 1 % selenium was about 95.5±6.38 % while wound closure of the negativecontrol group was about 48.83±4.03 %. Our results showed the capability of nanofibrous containing selenium for successfulwound treatment.

Factors influencing sperm retrieval following testicular sperm extraction in nonobstructive azoospermia patients

Salehi, Peyman,Derakhshan-Horeh, Marzieh,Nadeali, Zakiye,Hosseinzadeh, Majid,Sadeghi, Erfan,Izadpanahi, Mohammad Hossein,Salehi, Mansour The Korean Society for Reproductive Medicine 2017 Clinical and Experimental Reproductive Medicine Vol.44 No.1

Objective: Azoospermia owing to testicular disorders is the most severe manifestation of male infertility. The main concern for patients with nonobstructive azoospermia (NOA) is the probability of successful sperm retrieval following testicular sperm extraction (TESE). Therefore, the goal of this study was to determine predictive factors correlated with sperm retrieval. Methods: We assessed the testicular histopathological patterns, the choice of TESE surgical procedure, hormone levels, and chromosomal abnormalities in patients with NOA (n=170). The histopathology specimens were analyzed based on the histopathological patterns of hypospermatogenesis, maturation arrest, and Sertoli cell-only syndrome. Results: The mean rate of sperm retrieval was 48.8%. The rate of sperm retrieval was significantly higher in the hypospermatogenesis group than in the other groups (p<0.001). There was a positive correlation between micro-TESE (vs. conventional TESE) and the sperm retrieval rate (odds ratio, 8.077; p<0.01). A logistic regression model demonstrated that high levels of follicle-stimulating hormone (FSH) and small testicular volume were significantly associated with lower chances of successful sperm retrieval. Conclusion: Some parameters, including testicular histopathology patterns, FSH levels, testicular volume, and method of TESE surgery, may be able to predict the chances of obtaining spermatozoa in patients with NOA. However, despite the efficiency of some predictive models, the hope of retrieving any functioning spermatozoa may be sufficient to disregard predictive factors of the success of intracytoplasmic sperm injection in these patients.

Porous electrospun poly(e-caprolactone)/gelatin nanofi brous mat containing cinnamon for wound healing application: in vitro and in vivo study

Majid Salehi,Maryam Niyakan,Arian Ehterami,Saeed Haghi-Daredeh,Simin Nazarnezhad,Ghasem Abbaszadeh-Goudarzi,Ahmad Vaez,Seyedeh Fatemeh Hashemi,Nariman Rezaei,Seyed Reza Mousavi 대한의용생체공학회 2020 Biomedical Engineering Letters (BMEL) Vol.10 No.1

In this study, cinnamon (cin) was loaded into poly(ε-caprolactone)/gelatin (PCL/Gel) nanofi brous matrices in order to fabricatean appropriate mat to improve wound healing. Mats were fabricated from PCL/COLL [1:1 (w/w)] solution with 1, 5and 25% (w/v) of cinnamon. Prepared mats were characterized with regard to their microstructure, mechanical properties,porosity, surface wettability, water-uptake capacity, water vapor permeability, blood compatibility, microbial penetration andcellular response. The fabricated mats with and without cinnamon were used to treat the full-thickness excisional wounds inWistar rats. The results indicated that the amount of cinnamon had a direct eff ect on porosity, mechanical properties, wateruptake capacity, water contact angle, water vapor transmission rate and cell proliferation. In addition, the results of in vivostudy indicated that after 14 days, the wounds which were treated with PCL/Gel 5%cin had better wound closure (98%)among other groups. Our results suggest that the cinnamon can be used as a suitable material for wound healing.

Polyurethane/Gelatin Nanofiber Neural Guidance Conduit in Combination with Resveratrol and Schwann Cells for Sciatic Nerve Regeneration in the Rat Model

Majid Salehi,Arian Ehtrami,Farshid Bastami,Saeed Farzamfar,Sepanta Hosseinpour,Hamid Vahedi,Ahmad Vaez,Mostafa Rahvar,Arash Goodarzi 한국섬유공학회 2019 Fibers and polymers Vol.20 No.3

Peripheral nerve injury is a serious challenge which influences 2.8 percent of trauma patients. Tissue engineeringof peripheral nerves mainly focuses on axonal regeneration via various nerve guides. The aim of this study is to evaluate a novel polyurethane (PU)/gelatin nanofibers (GNFs) conduit’s potential combination with resveratrol (RVT) for sciatic nerve regeneration in the rat. Platelet-rich plasma (PRP) was used as a carrier for RVT. Different tests like contact angle, tensile strength etc. was used to evaluate properties of PU/GNFs conduits. In addition, the electron microscopy, MTT assay, and DAPI staining revealed its compatibility with Schwann cells. 24 male Wistar rats were allocated into four groups (n=6) (1) PU/GNF/PRP/Schwann cell, 2) PU/GNF/Schwann cell/PRP/RVT, 3) Positive control, and 4) Negative control in order to assess sciatic functional index (SFI), hot plate latency, electromyographical (EMG), the percentage of wet weight-loss of gastrocnemius muscle and histopathological studies using hematoxylin-eosin staining. The results represented sciatic functional index (SFI), hot plate latency, electromyographical improved significantly in group 1 and 2 compared to the negative control group. Histopathological findings showed remarkable improvements in myelin sheath regeneration and fibers condition in group 1 and 2 compared to the negative control group. Group 2 showed more resemblance to the normal sciatic nerve, with well-arranged fibers and an intact myelin sheath. This study successfully applied PU/GNFs/PRP/RVT conduits as a potential biocompatible nerve guide with proper mechanical properties, biocompatibility, and biodegradability that enhanced injured sciatic nerve’s recovery rate.

Naringin-loaded Poly(ε-caprolactone)/Gelatin Electrospun Mat as a Potential Wound Dressing: In vitro and In vivo Evaluation

Majid Salehi,Ahmad Vaez,Mahdi Naseri-Nosar,Saeed Farzamfar,Arman Ai,Jafar Ai,Shima Tavakol,Mehrdad Khakbiz,Somayeh Ebrahimi-Barough 한국섬유공학회 2018 Fibers and polymers Vol.19 No.1

In the present study, naringin, a flavonoid isolated from the grape and citrus fruit species, was incorporated with poly(ε-caprolactone)/gelatin composite mats in order to develop a potential wound dressing. The composite mats were prepared by electrospinning of poly(ε-caprolactone)/gelatin (1:1 (w/w)) solution incorporated with 1.50 %, 3 % and 6 % (w/w) of naringin. The electrospun mats were evaluated regarding their morphology, contact angle, water-uptake capacity, water vapor transmission rate, tensile properties, drug release, cellular response and in vivo wound healing activity. The study showed that after 2 weeks, the full-thickness excisional wounds of Wistar rats treated with the naringin-loaded dressings achieved a wound closure of higher than 94 % and the dressing containing 6 % (w/w) naringin had almost 100 %wound closure. The sterile gauze, as the control group, showed nearly 86 % of wound closure after this period of time. Our results provided evidence that supports the possible applicability of naringin-loaded wound dressing for successful wound treatment.

Comparative Study of Poly(L-Lactic Acid) Scaffolds Coated with Chitosan Nanoparticles Prepared via Ultrasonication and Ionic Gelation Techniques

Majid Salehi,Mahdi Naseri-Nosar,Mahmoud Azami,Saeedeh Jafari Nodooshan,Javad Arish 한국조직공학과 재생의학회 2016 조직공학과 재생의학 Vol.13 No.5

In this study, an attempt was made to develop bi-functional constructs serving both as scaffolds and potential delivery systems for application in neural tissue engineering. The constructs were prepared in two steps. In the first step, the bulks of poly (L-lactic acid) (PLLA) in 1, 4-dioxane/water (87:13) were fabricated using liquid-liquid thermally induced phase separation technique. In the next step, the prepared bulks were coated with chitosan nanoparticles produced by two different techniques of ultrasonication and ionic gelation by grafting-coating technique. In ultrasonication technique, the chitosan solution (2 mg/mL) in acetic acid/sodium acetate buffer (90:10) was irradiated by an ultrasound generator at 20 kHz and power output of 750 W for 100 s. In ionic gelation technique, the tripolyphosphate in water solution (1 mg/mL) was added to the same chitosan solution. The physicochemical properties of the products were characterized by Scanning Electron Microscopy, Attenuated Total Reflection Fourier Transform-Infrared, liquid displacement technique, contact angle measurement, compressive and tensile tests, as well as zeta potential and particle size analysis using dynamic light scattering. Moreover, the cell proliferation and attachment on the scaffolds were evaluated through human glioblastoma cell line (U-87 MG) and human neuroblastoma cell line [BE (2)-C] culture respectively. The results showed that the samples coated with chitosan nanoparticles prepared by ultrasonication possessed enhanced hydrophilicity, biodegradation and cytocompatibility compared with pure PLLA and PLLA coated with chitosan nanoparticles prepared by ionic gelation. This study suggests successful nanoparticles-scaffold systems which can act simultaneously as potential delivery systems and tissue engineering scaffolds.

Feasibility of hydrophobized PES membrane in hybrid MD/FO process using magnetic draw solution

Setareh Salehi,Mohsen Jahanshahi,Majid Peyravi 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.6

The feasibility of PES membrane with hydrophobic property, high glass transition temperature, excellentthermal and chemical stability, with good mechanical properties as porous polymeric support was studied in membranedistillation (MD) process. Several strategies were considered and investigated to decrease membrane wetting bykeeping up salt rejection and also to improve the permeate flux of PES membrane in the MD process. From this pointof view, hydrophobic PMHS polymer with different concentrations was used as a surface coating solution. The morphologyand structure of prepared membranes were evaluated by AFM and FESEM analyses. After coating modification,the salt rejection was increased up to 98% and the contact angle was changed from 65.11o to >100.2o. To increasethe water flux, the effect of feed temperature (55 oC and 75 oC) and draw solution utilization containing magneticnanoparticle (Fe3O4) was investigated. Using draw solution provided a driving force from feed solution to permeateside and increased average permeate flux from 2.9 kg/m2·h to 6.9 kg/m2·h in the 0.06 g/l of draw solute concentration. It improved permeate flux and salt rejection simultaneously.

Promotion of excisional wound repair by a menstrual blood-derived stem cell-seeded decellularized human amniotic membrane

Saeed Farzamfar,Majid Salehi,Arian Ehterami,Mahdi Naseri-Nosar,Ahmad Vaez,Amir-Hassan Zarnani,Hamed Sahrapeyma,Mohammad Reza Shokri,Mehdi Aleahmad 대한의용생체공학회 2018 Biomedical Engineering Letters (BMEL) Vol.8 No.4

This is the first study demonstrating the efficacy of menstrual blood-derived stem cell (MenSC) transplantation viadecellularized human amniotic membrane (DAM), for the promotion of skin excisional wound repair. The DAM wasseeded with MenSCs at the density of 3 9 104 cells/cm2 and implanted onto a rat’s 1.50 9 1.50 cm2 full-thicknessexcisional wound defect. The results of wound closure and histopathological examinations demonstrated that the MenSCseededDAM could significantly improve the wound healing compared with DAM-treatment. All in all, our data indicatedthat the MenSCs can be a potential source for cell-based therapies to regenerate skin injuries.

A promising wound dressing based on alginate hydrogels containing vitamin D3 cross‑linked by calcium carbonate/d‑glucono‑δ‑lactone

Arian Ehterami,Majid Salehi,Saeed Farzamfar,Hadi Samadian,Ahmad Vaez,Hamed Sahrapeyma,Sadegh Ghorbani 대한의용생체공학회 2020 Biomedical Engineering Letters (BMEL) Vol.10 No.2

In the present study, we fabricated vitamin D 3 -loaded alginate hydrogel and assessed its wound healing capability in the animalmodel. The various concentrations of vitamin D3 were added to the pre-dissolved sodium alginate in deionized water andcross-linked by calcium carbonate in combination with D -glucono-δ-lactone. The microstructure, swelling behavior, weightloss, hemo- and cytocompatibility of the fabricated hydrogels were evaluated. In the last stage, the therapeutic effi cacy ofthe prepared hydrogels was evaluated in the full-thickness dermal wound model. The scanning electron microscopy imagesshowed that the prepared hydrogel was highly porous with the porosity of 89.2 ± 12.5% and contained the interconnectedpores. Weight loss assessment showed that the prepared hydrogel is biodegradable with the weight loss percentage of about89% in 14 days. The results showed that the prepared hydrogels were hemo- and cytocompatible. The animal study resultsimplied that alginate hydrogel/3000 IU vitamin D 3 group exhibited the highest wound closure present which was statisticallysignifi cant than the control group ( p < 0.05). Moreover, the histological examinations revealed that hydrogel containing3000 IU vitamin D3 had the best performance and induced the highest re-epithelialization and granular tissue formation. Allin all, this study suggests that alginate hydrogels with 3000 IU vitamin D 3 can be exploited as a potential wound dressingin skin tissue engineering.

A novel hydrogel containing 4-methylcatechol for skin regeneration: in vitro and in vivo study

Jilla Majidi Ghatar,Arian Ehterami,Simin Nazarnezhad,Maryam Sadat Hassani,Nariman Rezaei Kolarijani,Solmaz Mahami,Majid Salehi 대한의용생체공학회 2023 Biomedical Engineering Letters (BMEL) Vol.13 No.3

Skin damages are usual physical injuries and different studies have been done to improve wound healing. Hydrogel dueto its properties like a moist environment and cooling wound site is a good option for wound treatment. In this study, weevaluated the consequence of using alginate/chitosan hydrogel contained various dosages of 4-Methylcatechol (0, 0.1, 1%(W/W)) on wound healing. After hydrogel fabrication, different tests like SEM, swelling, release, weight loss, and hemoandcytocompatibility were done to characterize fabricated hydrogels. Finally, the rat model was used to assess Alginate/Chitosan hydrogel's therapeutic function containing 0.1 and 1% of 4-Methylcatechol. The pore size of hydrogel was between24.5 ± 9 and 62.1 ± 11.63 μm and about 90% of hydrogel was lost after 14 days in the weight loss test. Blood compatibilityand MTT assay showed that hydrogels were nontoxic and improved cell proliferation. In vivo test showed that Alginate/Chitosan/0.1%4-Methylcatechol improved wound healing and the results were significantly better than the gauze-treatedwound. Our results showed dose depending effect of 4-Methylcatechol on wound healing. This study shows the treatmenteffect of 4-Methylcatechol on wound healing and the possibility of using it for treating skin injuries.