Studies on the Yeast - Derived Hepatitis B Vaccine

Mahn Hoon Park,Sung Jin Kim,Man Bock Gu,Kyung Hwan Jung,Hee Bok Song,Kyong Nam Park,Kyong Ho Kim 대한바이러스학회 1988 Journal of Bacteriology and Virology Vol.18 No.1

증기발생기 전열관 2차측 이물질 실증실험

민경만(Kyong-mahn Min),김현희(Hyun-hee Kim),권석훈(Seok-hoon Kwon),권진욱(Jin-wook Kwon),박중암(Jung-am Park) 한국비파괴검사학회 2022 한국비파괴검사학회 춘계학술대회 초록집 Vol.2022 No.5

단순지지된 두 원판의 유체연성 고유진동 해석

정경훈(Kyeong-Hoon Jeong),이규만(Gye-Mahn Lee),박근배(Keun-Bae Park) 한국소음진동공학회 2001 한국소음진동공학회 학술대회논문집 Vol.2001 No.11

인과지도 분석을 통한 긍정심리자본과 21세기 정치리더십의 재고찰

박상만 ( Sang Mahn Park ),김강훈 ( Gang Hoon Kim ) 한국시스템다이내믹스학회 2013 한국시스템다이내믹스 연구 Vol.14 No.4

The main purpose of this research is to introduce PsyCap(Positive Psychological Capital) and new leadership helping ordinary people to create positive thinking and leading to social integration in South Korea. Additionally, by looking at conventional leadership theories and by applying the PsyCap to leadership based on causal loop analysis, this study is to seek new approach which political leader is able to communicate with the people and can help the people to build positive mine. Throughout this study, there are several important implications how political leader can lead to social integration and stable political development in South Korea. In particular, when political leader encourage the people to have confidence to take on and put in the necessary effort to succeed at challenging tasks, when political leader make the people a positive attribution (optimism) about succeeding now and in the future, when political leader help the people to persevere toward goals and redirection paths to goals (hope) in order to succeed, and when political leader make the people beset by problems and adversity environment to overcome these concerns, it can be expected political leader to build social integration and the people to create positive thinking which has been emphasized by political leader in South Korea. Finally, 21st century has required new political leadership that the people are able to make positive psychological capital composed by optimism, hope, confidence, and resilience.

전류신호 분석을 통한 저널베어링 이상상태 진단

박진석(Jin Seok Park),허형(Hyung Huh),정경훈(Kyeong Hoon Jeong),이규만(Kyu Mahn Lee),박근배(Keun Bae Park) 한국소음진동공학회 2005 한국소음진동공학회 학술대회논문집 Vol.2005 No.11

Dynamic Characteristics of the Integral Reactor SMART

Kim, Tae-Wan,Park, Keun-Bae,Jeong, Kyeong-Hoon,Lee, Gyu-Mahn,Park, Suhn Korean Nuclear Society 2001 Nuclear Engineering and Technology Vol.33 No.1

In this study, a dynamic analysis of the integral reactor SMART (System-integrated Modular Advanced ReacTor) under postulated seismic events is performed to review the response characteristics of the major components. To enhance the feasibility of an analysis model, a detailed finite element model is synchronized with the products of concurrent design activities. The artificial time history, which has been applied to the seismic analysis for the Korean Standard Nuclear Power Plant (KSNP), is chosen to envelop broad site specifics in Korea. Responses in the horizontal direction are found slightly amplified, while those in the vertical direction are suppressed. Since amplified response is monitored at the control element drive mechanism (CEDM), minor design provision is considered to enhance the integrity of the subsystem.

Studies on the Yeast-Derived Hepatitis B Vaccine

Jung, Kyong-Hwan,Park, Kyong-Nam,Park, Mahn-Hoon,Kim, Sung-Jin,Song, Hee-Bok,Gu, Man-Bock,Kim, Kyong-Ho INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1989 YONSEI REPORTS ON TROPICAL MEDICINE Vol.20 No.1

The yeast s. cerevisiae transformed with yeast expression vector (pMGGalphaH) produce HBsAg consistently, vHBsAg was purified form this yeast and formulated into the yeast derived hepatitis B vaccine. In this article, we conducted several studies on the characteristics of yHBsAg and on the immunogenecity of yeast-derived hepatitis B vaccine. The results are summarized as follows. 1) The physico-chemical characteristics of purified yHBsAg checked by electron microscopic observation, ultraviolet absorption analysis and ultracentrifugational analysis were as same those of hGHs Ag. 2) The purity of purified yHBsAg checked by SDS-PAGE and HPLC was above 98% and antigenecity checked by immunodiffusion test was same as hHBsAg. 3) The immunogenecity of yeast derived hepatitis B vaccine was identical to that of plasma-derived HB vaccine in mice and guinea pigs. ED50% of yeast HB vaccine in mice was 0.3125㎍/㎖. 4) Seroconversion rate of volunteers vaccinated with 20㎍/dose of yeast-derived HB vaccine was 32.8% at month after first injection, 64.1% at 2 months, 91.5% at 6 months and 98.4% at 7 months. The result of this studies indicate that yeast-derived hepatitis B vaccine developed at Mogam Biotechnology Research Institute is safe and immunogenic for man

양면에서 부분적으로 유체와 접하는 사각평판의 고유진동

정경훈(Jeong, Kyeong-Hoon),이규만(Lee, Gyu-Mahn),김태완(Kim, Tae-Wan),박근배(Park, Keun-Bae) 한국소음진동공학회 2008 한국소음진동공학회 논문집 Vol.18 No.1

An analytical method for the free vibration of a flexible rectangular plate in contact with water is developed by the Rayleigh-Ritz method. The plate clamped along the edges is partially contacted with water at both sides. It is assumed that the contained water is incompressible and inviscid. The wet mode shape of the plate is assumed as a combination of the dry mode shapes of a clamped beam. The liquid motion is described by using the liquid displacement potential and determined by using the compatibility conditions along the liquid interface with the plate. Minimizing the Rayleigh quotient based on the energy conservation gives an eigenvalue problem. It is found that the theoretical results can predict excellently the fluid-coupled natural frequencies comparing with the finite element analysis result.

쥐의 폐에서 방사선이 Nitric Oxide (NO), Nitric Oxide Syntahse (NOS) 및 TGF-β의 발현에 미치는 영향

오영택(Oh Young-Taek),박광주(Kwang-Joo Park),길훈종(Hoon-Jong Kil),하만준(Mahn Joon Ha),전미선(Mison Chun),강승희(Seung-Hee Kang),박성은(Seong-Eun Park),장세경(Sei-Kyung Chang) 대한방사선종양학회 2000 Radiation Oncology Journal Vol.18 No.4

목적 : 방사선폐렴에서 NO가 염증반응을 매개하는 물질로 보고되고 있는데, 이때 유도형의 Nitric Oxide Synthase2 (NOS2)가 주로 Nitric Oxide (NO) 생성에 관여하는 것으로 알려져 있다. 이 NOS2의 억제조절물질로 TGF-β가 알려져 있으며, 최근에는 NO가 증가하면 TGF-β의 활성이 증가하고 그에 따라 NOS2가 억제되어 NO의 생성이 억제되는 간섭기전(feedback mechanism)이 보고되어 있다. In vivo 동물모델에서 방사선조사 후에 NOS2 발현 및 NO 생성의 변화를 측정하고, 그에 따라 TGF-β가 어떻게 변하는지를 관찰하고자 본 연구를 진행하였다. 대상 및 방법 : Sprague-dawley 쥐 60마리를 마취 후 5 Gy와 20 Gy의 방사선을 우측 폐에 조사하고 대조군은 위 조사를 시행하였다. 방사선조사 후 3일, 7일, 14일, 28일, 56일에 방사선 조사군 (5 Gy, 20 Gy0 각 5마리와 대조군 2마리를 희생시키고, 희생시키기 전에 우측 폐와 좌측 폐의 주기관지에서 각각 폐포 세척을 시행하여 폐포 세척액을 얻었다. 폐포 세척액에서 단백질과 세포 수를 측정한 후 원심 분리하여 상층액은 NO와 TGF-β 단백의 양을 측정하는데, 가라앉은 폐포 대식세포는 TGF-β와 NOS2 mRNA의 RT-PCR을 위한 검체로 사용하였다. 결과 : 방사선조사 후 5 Gy군에서 NOS2 mRNA가 14일 째에 우측 폐에서 발견하였고, 28일 째에는 양쪽 폐에서, 56일 째에는 좌측 폐에서 증가하였다. TGF-β mRNA는 계속 발현되었으나 28일 째에 발현이 감소하는 양상이었고 56일에는 좌측 폐에서 증가하였다. 폐에서 증가하였다. 20 Gy 군에서는 NOS2 mRNA는 5 Gy와 마찬가지로 14일주 째에 우측 폐에서 발현하여 28일주째에 양쪽 폐에서 발현하고 56일 째에 양쪽 폐에서 강하게 발현하였다. TGF-β mRNA는 28일 째에 우측 폐에서 56일 째에 양측 폐에서 증가하였다. 폐포 세척액 내의 NO의 양은 28일 째에 양쪽 폐에서 증가하였고, TGF-β 단백의 양은 우측 폐에서 28일 째 정점을 이루고, 56일 째에 좌측 폐에 비해 유의한 증가 양상을 보여주었다. 결론 : 방사선폐렴의 동물모델에서 NO, NOS2와 TGF-β는 선량에 따라 약간의 차이가 있었지만 14일 째 우측 폐에서의 NOS2 mRNA의 발현을 시작으로 비교적 일관된 발현 양상을 보여 주었고 그에 따라 폐포세척액 내의 NO 및 TGF-β 단백의 양이 변하였다. 그러나, 예상과는 달리 56일 째에는 NOS2와 TGF-β가 모두 발현하여, 좀 더 많은 개체 수를 대상으로 장기간을 통해 발현 양상을 관찰할 필요성이 있으며, 이 연구를 바탕으로 NOS2 억제제를 사용하여 TGF-β의 발현 양상에 어떤 변화가 오는지에 대한 연구가 필요하다고 평가되었다. Purpose : NOS2 induce NO production and NO activate TGF-β. The TGF-β is a inhibitor of NOS2. If this negative feedback mechanism operation in radiation pneumonitis model, NOS2 inhibitor may play a role in TGF-β suppression. We planned this study to evaluate the expression patterns of NO, NOS2 and TGF-β in vivo radiation pneumonitis model. Materials and Methods : Sixty sprague-Dawley rat were irradiated 5 Gy or 20 Gy. They were sacrificed 3, 7. 14. 28 and 56 days after irradiation. During sacrifice, we performed broncho-alveolar lavage (BAL). The BAL fluids were centrifuged and supernatents were used for measure NO and TGF-β, and the cells were used for RT-PCR. Results : After 5 Gy of radiation, NO in BAL fluid increased at 28 days in both lung and TGF-β I left lung at 56 days. NO increased in BAL fluid at 28 days in both lung after irradiation and TGF-β in right lung at 28-56 days after 20 Gy of radiation. After 5 Gy of radiation, NOS2 expression was increased in right lung at 14 days, in both lung at 28 days and in left lung at 56 days, TGF-β expression was reduced in both lung at 28 days and increased in left lung at 56 days. Conclusions : The proposed feedback mechanism of NO, NOS2 and TGF-β was operated in vivo radiation pneumonitis model. At 56 days, however, NOS2 and TGF-β expressed concurrently in left lung after 5 Gy and in both lung after 20 Gy of radiation.

자살예방 정책수단 평가 및 해결방안 연구

김정기(Jung-Ki Kim),박상만(Sang-Mahn Park),김강훈(Gang-Hoon Kim) 한국산업경제학회 2017 산업경제연구 Vol.30 No.4

본 연구에서는 핀란드와 일본의 자살예방프로그램에 대한 경제적 분석 및 정책평가를 실시하였다. 본 연구 분석결과를 요약하면 다음과 같다. 먼저 자살예방프로그램을 강력하게 시행한 국가들은 정책추진사후에 자살증가가 감소하고, 그 변동성이 안정적 상태로 나타났다. 둘째, 일본과 핀란드의 자살예방프로그램의 정책효과를 정량적으로 평가할 때, 평균 연간 자살률이 전년대비 14.2%감소되는 효과가 존재하였다. 셋째, 정부의 자살예방프로그램은 경제적인 요인뿐만 아니라 사회적인 요인을 포함하는 광범위한 정책수단을 추진할 때, 정책효과의 유효성이 나타났다. 본 연구 분석결과에 나타난 자살예방프로그램시행에 대한 정책적 시사점은 다음과 같다. 먼저 자살예방프로그램추진에 따른 긍정적 효과가 존재하기 때문에 장기적이고 지속적인 정책추진력이 중요한 요소임을 보여준다. 둘째, 정부차원의 자살예방프로그램이 성공하기 위해서는 광범위한 관련 영역 즉 경제영역과 사회영역 그리고 문화영역을 포함하는 정책적용범위가 요구된다. 마지막으로 자살예방프로그램의 정책효과를 극대화하기 위해서는 자살예방프로그램에 관련된 대상을 지속적으로 모니터링하고, 장기적 차원에서 빅 데이터 및 기계학습을 적용하는 단계가 요구된다. In this study, we conducted an economic analysis and policy evaluation of suicide prevention program. The results of this study are summarized as follows. First, the countries that implemented the suicide prevention program strongly showed that the suicide rate decreased after the implementation of the policy and that the volatility was stable. Second, when quantitatively evaluating the policy effects of suicide prevention programs in Japan and Finland, the average annual suicide rate decreased by 14.2% from the previous year. Third, the government"s suicide prevention program showed the effectiveness of the policy effect when pursuing a wide range of policy instruments including economic factors as well as social factors. The results of this study suggest that the policy implications of suicide prevention programs are as follows. First, there is a positive effect of the suicide prevention program, which shows that long-term and sustained policy drive is an important factor. Second, in order to success the suicide prevention programs at the government, ranges of policy coverages including economic, social and cultural areas are required. Finally, in order to maximize the policy effect of the suicide prevention program, it is necessary to continuously monitor the subjects related to the suicide prevention program and to apply the big data and machine learning in the long term.