Prognostic Role of MicroRNA-21 in Non-small Cell Lung Cancer: a Meta-analysis

Ma, Xue-Lei,Liu, Lei,Liu, Xiao-Xiao,Li, Yun,Deng, Lei,Xiao, Zhi-Lan,Liu, Yan-Tong,Shi, Hua-Shan,Wei, Yu-Quan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Introduction: Many studies have reported that microRNA-21 (miR-21) mihght predict the survival outcome in non-small cell lung cancers (NSCLCs) but the opposite opinion has also been expressed. The aim of this study was to summarize the evidence for a prognostic role of miR-21. Materials and Methods: All the eligible studies was searched by Medline and EMBASE and patients' clinical characteristics and survival outcome were extracted. Then a meta-analysis was performed to clarify the prognostic role of the miR-21 expression in different subgroups. Results: A total of 8 eligible articles were yielded covering survival outcomes or clinical characteristics. The combined hazard ratio (HR) and 95% confidence interval (95% CI) for overall survival (OS) was 2.19 [0.76, 6.30], while the combined HR (95% CI) of Asian group for OS had a significant result, 5.49 [2.46, 12.27]. The combined HR (95% CI) for recurrence free survival or disease free survival (RFS/DFS) was 2.31 [1.52, 3.49]. Odds ratios (ORs) showed that the miR-21 expression was associated with lymph node status and histological type. Conclusion: miR-21 expression could predict the prognostic outcome of NSCLC in Asians, despite some deficiencies in the study data.

Effects of the molecular weight and chemical properties of Schizophyllum commune polysaccharides on their immunomodulatory activity

Ma Nan,Tae Jin Park,Zhang Yu Tong,Khamphone Yelithao,Sang Guan You 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

The Unidirectional Wettability Property of a New Warp-knitted Double-face Fabric

Tong Yang,Haifan Yu,Yuying Liu,Zhijia Dong,Pibo Ma 한국섬유공학회 2020 Fibers and polymers Vol.21 No.8

In order to knit a warp-knitted fabric with unidirectional wettability property, polyester multifilament and cottonyarn were used as raw materials, and samples were produced on an RD6 EL warp-knitting machine. The unidirectionalwettability property of the fabric was tested by measuring the length of artificial sweat absorbed in the fabric. And theunidirectional wettability of the fabric primarily depends on the structure and twist of the yarn, especially the twist. Themoisture absorption capacity of the yarn decreases with the yarn twist increasing. When the twist reaches a certain value, itwill prevent the entry of moisture. This provides us with an inspiration for controlling the moisture absorption of fabric by thetwist of the yarn when knitting the unidirectional wettability fabric.

Effect of different tooth preparation designs on the marginal and internal fit discrepancies of cobalt-chromium crowns produced by computer-aided designing and selective laser melting processes

Na Yu,Hong-Wei Dai,Fa-Bing Tan,Jin-Lin Song,Chao-Yi Ma,Xue-Lu Tong 대한치과보철학회 2021 The Journal of Advanced Prosthodontics Vol.13 No.5

PURPOSE. To evaluate the impact of five different tooth preparation designs on the marginal and internal fit discrepancies of cobalt-chromium (CoCr) crowns produced by computer-aided designing (CAD) and selective laser melting (SLM) processes. MATERIALS AND METHODS. Five preparation data were constructed, after which design crowns were obtained. Actual crowns were fabricated using an SLM process. After the data of actual crowns were obtained with structural light scanning, intaglio surfaces of the design crown and actual crown were virtually superimposed on the preparation. The fit-discrepancies were displayed with colors, while the root means square was calculated and analyzed with one-way analysis of variance (ANOVA), Tukey’s test or Kruskal-Wallis test (α = .05). RESULTS. The marginal or internal color-coded images in the five design groups were not identical. The shoulder-lip and sharp line angle groups in the CAD or SLM process had larger marginal or internal fit discrepancies compared to other groups (P < .05). In the CAD process, the mean marginal and internal fit discrepancies were 10.0 to 24.2 μm and 29.6 to 31.4 μm, respectively. After the CAD and SLM processes, the mean marginal and internal fit discrepancies were 18.4 to 40.9 μm and 39.1 to 47.1 μm, respectively. The SLM process itself resulted in a positive increase of the marginal (6.0 - 16.7 μm) and internal (9.0 - 15.7 μm) fit discrepancies. CONCLUSION. The CAD and SLM processes affected the fit of CoCr crowns and varied based on the preparation designs. Typically, the shoulder-lip and sharp line angle designs had a more significant effect on crown fit. However, the differences between the design groups were relatively small, especially when compared to fit discrepancies observed clinically.

Enhancement of the electro-Fenton degradation of organic contaminant by accelerating Fe3+/Fe2+ cycle using hydroxylamine

Dong Li,Tong Zheng,Jianghua Yu,Haiyang He,Wei Shi,Jun Ma 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.105 No.-

The Electro-Fenton process can generate reactive oxygen species capable of oxidizing refractory organiccontaminants. However, low regeneration efficiency of Fe2+ restricts its application. Herein, hydroxylamine(HA) was added into the Electro-Fenton (HA/Electro-Fenton) process to accelerate the transformationof Fe3+ to Fe2+. Using dimethyl phthalate (DMP) as target contaminant, the HA/Electro-Fenton systemalleviated the two-stage reaction process and accelerated the removal of DMP in the pH range of 2.0–6.0. With improving DMP concentration from 5 mg L-1 to 50 mg L-1, their degradation rate increased in theHA/Electro-Fenton system, while decreased in the Electro-Fenton system. The addition of HA had negligibleeffect on electro-generation of H2O2, but facilitate the redox cycle of Fe3+/Fe2+ and the generation ofhydroxyl radicals, thus improving the degradation of DMP. The final transformation products of HA wereN2, N2O, and NO3. The presence of PO4 3 improved DMP degradation, while Cl and organic matters inhibitedDMP removal in varying degrees. This study provided useful reference to solve the low efficiency ofFe3+/Fe2+ cycle and expand the pH application range in the Electro-Fenton process.

A novel M2e-multiple antigenic peptide providing heterologous protection in mice

Feng Wen,Ji-Hong Ma,Hai Yu,Fu-Ru Yang,Meng Huang,Yan-Jun Zhou,Ze-Jun Li,Xiu-Hui Wang,Guo-Xin Li,Yi-Feng Jiang,Wu Tong,Guangzhi Tong 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.1

Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.

Development of manganese ferrite/graphene oxide nanocomposites for magnetorheological fluid with enhanced sedimentation stability

Guangshuo Wang,Yingying Ma,Yu Tong,Xufeng Dong 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.48 No.-

Novel nanocomposites consisting of manganese ferrite nanoparticles and graphene oxide nanosheets(MnFe2O4/GO) have been synthesized as a promising candidate for magnetorheological (MR)fluid. Themorphology, microstructure, composition and magnetic properties of the obtained MnFe2O4/GO werestudied in detail. It was found that the MnFe2O4 nanoparticles with diameter of 8–12 nm were denselydecorated on the surface of GO nanosheets. The magnetization investigation revealed that as-preparedMnFe2O4/GO had superparamagnetic behavior with saturation magnetization of 36.2 emu/g. The MRfluid was prepared by the obtained MnFe2O4/GO and the corresponding MR properties were investigatedusing a Physica MCR301 rheometerfitted with a magneto-rheological module. The MnFe2O4/GO-basedMRfluid exhibited typical MR effect with increasing shear stress, yield stress and dynamic shear modulusdepending on magneticfields. More importantly, the sedimentation stability of the prepared MRfluidwas found to be improved due to the unique sheet-like structure and the reduced density mismatchbetween the dispersed particles and the carrier medium. The MnFe2O4/GO-basedfluid with typical MReffect and excellent sedimentation stability would provide a feasible candidate for practical applications

Functional and Mechanistic Characterization of PRMT6- Regulated Autophagy in Hepatocellular Carcinoma

( Noelia Che ),( Kai Yu Ng ),( Man Tong ),( Michael Sy Huen ),( Xin Yuan Guan ),( Stephanie Ma ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Autophagy is a critical survival factor for cancer cells, whereby it maintains cellular homeostasis including degradation of damaged organelles and unwanted proteins as well as the support of cellular biosynthesis in response to environmental stress, preventing cells from undergoing apoptosis. We investigated the functional role of protein arginine methyltransferase 6 (PRMT6) in regulation of autophagy and sorafenib resistance, aiming to provide novel therapeutic insights for HCC. Methods: We characterised the regulatory role of PRMT6 in autophagy by immunofluorescence puncta staining, transmission electron microscopy and immunoblot analyses. Identification and validation of potential PRMT6-interacting partners were performed through tandem affinity purification coupled with mass spectrometry profiling followed by co-immunoprecipitation. Subsequent in vitro and in vivo methylation assays found PRMT6 to methylate its binding partners to mediate arginine methylation for post-translational modification of proteins. Lentiviral-based overexpression and knockdown approaches were utilised to examine and explore the functional role of PRMT6-downstream effector in PRMT6-mediated autophagy deregulations in HCC. Results: Upon autophagy induction by Earle’s Balanced Salt Solution (EBSS) to mimic nutrient deprivation, hypoxia to mimic oxygen deprivation and sorafenib treatment, we demonstrated a negative correlation between expression of PRMT6 and LC3BII in HCC. Intriguingly, we identified and validated a number of autophagy-related proteins from mass spectrometry-based proteomics, including Bcl-2 associated athanogene 5 (BAG5), as PRMT6-binding partners. Mechanistically, PRMT6 methylates BAG5, leading to its protein degradation. We later confirmed that, BAG5, a downstream effector of PRMT6, promotes HCC tumorigenesis through autophagic alterations in vitro and in vivo. More importantly, data-mining in The Cancer Genome Atlas (TCGA) - Liver Cancer dataset found patients with higher BAG5 expression to display a significantly worst survival outcome, indicating its potential translational values. Conclusions: Our findings suggest PRMT6 down-regulation in HCC tumors to promote tumorigenicity and sorafenib resistance through an altered autophagic flux via BAG5 de-regulation.

ATM Signaling Pathway Is Implicated in the SMYD3-mediated Proliferation and Migration of Gastric Cancer Cells

Wang, Lei,Wang, Qiu-Tong,Liu, Yu-Peng,Dong, Qing-Qing,Hu, Hai-Jie,Miao, Zhi,Li, Shuang,Liu, Yong,Zhou, Hao,Zhang, Tong-Cun,Ma, Wen-Jian,Luo, Xue-Gang The Korean Gastric Cancer Association 2017 Journal of gastric cancer Vol.17 No.4

Purpose: We previously found that the histone methyltransferase suppressor of variegation, enhancer of zeste, trithorax and myeloid-nervy-deformed epidermal autoregulatory factor-1 domain-containing protein 3 (SMYD3) is a potential independent predictive factor or prognostic factor for overall survival in gastric cancer patients, but its roles seem to differ from those in other cancers. Therefore, in this study, the detailed functions of SMYD3 in cell proliferation and migration in gastric cancer were examined. Materials and Methods: SMYD3 was overexpressed or suppressed by transfection with an expression plasmid or siRNA, and a wound healing migration assay and Transwell assay were performed to detect the migration and invasion ability of gastric cancer cells. Additionally, an MTT assay and clonogenic assay were performed to evaluate cell proliferation, and a cell cycle analysis was performed by propidium iodide staining. Furthermore, the expression of genes implicated in the ataxia telangiectasia mutated (ATM) pathway and proteins involved in cell cycle regulation were detected by polymerase chain reaction and western blot analyses. Results: Compared with control cells, gastric cancer cells transfected with si-SMYD3 showed lower migration and invasion abilities (P<0.05), and the absence of SMYD3 halted cells in G2/M phase and activated the ATM pathway. Furthermore, the opposite patterns were observed when SMYD3 was elevated in normal gastric cells. Conclusions: To the best of our knowledge, this study provides the first evidence that the absence of SMYD3 could inhibit the migration, invasion, and proliferation of gastric cancer cells and halt cells in G2/M phase via the ATM-CHK2/p53-Cdc25C pathway. These findings indicated that SMYD3 plays crucial roles in the proliferation, migration, and invasion of gastric cancer cells and may be a useful therapeutic target in human gastric carcinomas.

The purified extract of steamed Panax ginseng protects cardiomyocyte from ischemic injury via caveolin-1 phosphorylation-mediating calcium influx

Hai-Xia Li,Yan Ma,Yu-Xiao Yan,Xin-Ke Zhai,Meng-Yu Xin,Tian Wang,Dong-Cao Xu,Yu-Tong Song,Chun-Dong Song,Cheng-Xue Pan 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.6

Background: Caveolin-1, the scaffolding protein of cholesterol-rich invaginations, plays an important rolein store-operated Ca2þ influx and its phosphorylation at Tyr14 (p-caveolin-1) is vital to mobilize protectionagainst myocardial ischemia (MI) injury. SOCE, comprising STIM1, ORAI1 and TRPC1, contributesto intracellular Ca2þ ([Ca2þ]i) accumulation in cardiomyocytes. The purified extract of steamed Panaxginseng (EPG) attenuated [Ca2þ]i overload against MI injury. Thus, the aim of this study was to investigatethe possibility of EPG affecting p-caveolin-1 to further mediate SOCE/[Ca2þ]i against MI injury in neonatalrat cardiomyocytes and a rat model. Methods: PP2, an inhibitor of p-caveolin-1, was used. Cell viability, [Ca2þ]i concentration were analyzedin cardiomyocytes. In rats, myocardial infarct size, pathological damages, apoptosis and cardiac fibrosiswere evaluated, p-caveolin-1 and STIM1 were detected by immunofluorescence, and the levels ofcaveolin-1, STIM1, ORAI1 and TRPC1 were determined by RT-PCR and Western blot. And, release of LDH,cTnI and BNP was measured. Results: EPG, ginsenosides accounting for 57.96%, suppressed release of LDH, cTnI and BNP, and protectedcardiomyocytes by inhibiting Ca2þ influx. And, EPG significantly relieved myocardial infarct size, cardiacapoptosis, fibrosis, and ultrastructure abnormality. Moreover, EPG negatively regulated SOCE viaincreasing p-caveolin-1 protein, decreasing ORAI1 mRNA and protein levels of ORAI1, TRPC1 and STIM1. More importantly, inhibition of the p-caveolin-1 significantly suppressed all of the above cardioprotectionof EPG. Conclusions: Caveolin-1 phosphorylation is involved in the protective effects of EPG against MI injury viaincreasing p-caveolin-1 to negatively regulate SOCE/[Ca2þ]i.