Antibacterial potential of the extracts derived from leaves and in vitro raised calli of medicinal plants Pterocarpus marsupium Roxb., Clitoria ternatea L., and Sanseveiria cylindrica Bojer ex Hook

M Shahid,A Shahzad,M Anis 경희대학교 융합한의과학연구소 2009 Oriental Pharmacy and Experimental Medicine Vol.9 No.2

Pterocarpus marsupium, Clitoria ternatea, and Sanseveiria cylindrica are some of the important and endangered medicinal plant species of India. Despite of medicinal properties, antibacterial potential of the plants have not yet been explored. The present study was designed to optimize the in vitro technique for micropropagation and to screen the extracts from leaves and in vitro raised calli for antibacterial properties. Excised leaf-explants from the parent plants were surface sterilized and cultivated on Murashige & Skoog’s (MS) medium containing N6-benzyladenine (BA) in concentrations of 1, 2, 5, and 10 μM. Optimal growth of calli was noticed at a concentration of 5 μM, therefore the extracts from calli grown at this concentration were further studied for antibacterial activity. Both alcoholic and aqueous extracts from leaves of respective plants, and their in vitro raised calli were tested for antibacterial activity by agar well diffusion method against a range of Gram-positive and Gram-negative bacteria. Aqueous extracts showed antibacterial activity against limited number of bacterial species; notably the extracts of C. ternatea which showed antibacterial activity against Streptococcus pyogenes, Bacillus subtilis and Bacillus cereus. Alcoholic extracts of all three plants showed antibacterial activity against a wider range of bacteria. Among the Gram-positive bacteria, extracts from C. ternatea showed strong antibacterial activity against Bacillus spp., whereas the extracts of S. cylindrica showed good antibacterial potential for Staphylococcus aureus, S. epidermidis and S. pyogenes. The extracts from all three plants showed antibacterial activity against Gram-negative bacteria, including, Salmonella spp. and Shigella dysenteriae; organisms causing enteric fever and dysentery. In most of the cases, the extracts from respective calli showed comparable, and in some cases better, result in comparison to the extracts from parent leaves. To the best of our knowledge this is the first preliminary report on antibacterial potential, especially through calli extracts, of these plants; and in vitro cultivation of the explants may be used to obtain phytotherapeutic compounds. Pterocarpus marsupium, Clitoria ternatea, and Sanseveiria cylindrica are some of the important and endangered medicinal plant species of India. Despite of medicinal properties, antibacterial potential of the plants have not yet been explored. The present study was designed to optimize the in vitro technique for micropropagation and to screen the extracts from leaves and in vitro raised calli for antibacterial properties. Excised leaf-explants from the parent plants were surface sterilized and cultivated on Murashige & Skoog’s (MS) medium containing N6-benzyladenine (BA) in concentrations of 1, 2, 5, and 10 μM. Optimal growth of calli was noticed at a concentration of 5 μM, therefore the extracts from calli grown at this concentration were further studied for antibacterial activity. Both alcoholic and aqueous extracts from leaves of respective plants, and their in vitro raised calli were tested for antibacterial activity by agar well diffusion method against a range of Gram-positive and Gram-negative bacteria. Aqueous extracts showed antibacterial activity against limited number of bacterial species; notably the extracts of C. ternatea which showed antibacterial activity against Streptococcus pyogenes, Bacillus subtilis and Bacillus cereus. Alcoholic extracts of all three plants showed antibacterial activity against a wider range of bacteria. Among the Gram-positive bacteria, extracts from C. ternatea showed strong antibacterial activity against Bacillus spp., whereas the extracts of S. cylindrica showed good antibacterial potential for Staphylococcus aureus, S. epidermidis and S. pyogenes. The extracts from all three plants showed antibacterial activity against Gram-negative bacteria, including, Salmonella spp. and Shigella dysenteriae; organisms causing enteric fever and dysentery. In most of the cases, the extracts from respective calli showed comparable, and in some cases better, result in comparison to the extracts from parent leaves. To the best of our knowledge this is the first preliminary report on antibacterial potential, especially through calli extracts, of these plants; and in vitro cultivation of the explants may be used to obtain phytotherapeutic compounds.

PHA-Induced Peripheral Blood Cytogenetics and Molecular Anslysis : a Valid Diagnostic and Follow-up Modality For Acute Primyelocytic Leukemia Patients Treated With ATRA and/or Arsenic Tri-oxide

Baba, Shahid M,Azad, Niyaz A,Shah, Zaffar A,Afroze, Dil,Pandith, Arshad A,Jan, Aleem,Aziz, Sheikh A,Dar, Fayaz A Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Background: Acute promyelocytic leukemia (APML) is characterized by the reciprocal translocation t(15;17) (p22;p12) resulting in the PML-$RAR{\alpha}$ fusion gene. A dual diagnostic and follow up approach was applied including cytogenetic demonstration of the t(15;17) translocation and detection dg PML-$RAR{\alpha}$ chimeric transcripts by molecular means. Purpose: Conventional cytogenetics involving bone marrow is beset with high probability of poor metaphase index and was substituted with phytohemagglutinin (PHA)-induced peripheral blood culture based cytogenetic analysis as a diagnostic & follow up modality in APML patients of Kashmir (North India). Both qualitative (RT-PCR) and quantitative (Q-PCR) tests were simultaneously carried out to authenticte the modified cytogenetics. Materials and Method: Patient samples were subjected to the said techniques to establish their baseline as well as follow-up status. Results: Initial cytogenetics revealed 30 patients (81%) Positive for t(15;17) whereas 7 (19%) had either cryptic translocation or were negative for t(15;17). Two cases had chromosome 16q deletion and no hallmark translocation t(15;17). Q-PCR status for PML-$RAR{\alpha}$ was found to be positive for all patients. All the APML patients were reassessed at the end of consolidation phase and during maintenance phase of chemotherapy where 6 patients had molecular relapse, wherein 4 also demonstrated cytogenetic relapse. Conclusions: It was found that PHA-induced peripheral blood cytogenetics along with molecular analysis could prove a reliable modality in the diagnosis and assessment of follow up response of APML patients.

Assessment of Spatiotemporal Variability of Meteorological Droughts in Northern Iraq Using Satellite Rainfall Data

Hafed Qasem Ahmad,Samira A. Kamaruddin,Sobri B. Harun,Nadhir Al-Ansari,Shamsuddin Shahid,Ruqaya M. Jasim 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.11

The absence of a dense rainfall monitoring network and longer period data are the major hindrances of hydroclimatic study in arid and semi-arid regions. An attempt has been made for the evaluation of spatiotemporal changes in droughts at the northern semi-arid region of Iraq for the period 1981 − 2018 using high-resolution (0.05°) precipitation data of Climate Hazards Group Infrared Precipitation with Stations (CHIRPS). The performance of CHIRPS in replicating rainfall and Standard Precipitation Index (SPI) for different timescales at eleven locations for the available period of observation data (2000 − 2014) was evaluated. The SPI was also used to estimate drought frequency and evaluate drought trends at all the CHIRPS grid points. A modified version of the non-parametric Mann-Kendall (MK) test was employed for a robust evaluation of the spatial distribution of temporal trends in droughts. The results showed a good ability of CHIRPS in reconstructing observed SPI with a correlation coefficient ranged from 0.64 to 0.87, BIAS between 1.05 and 1.81, Nash-Sutcliff efficiency from 0.39 to 0.55, and Willmott Index between 0.67 and 0.79. The CHIRPS also able to reconstruct the time series and probability distribution of observed SPI reasonably. Spatial distribution of droughts revealed a higher frequency of droughts of all categories and timescales in the east and north of Northern Iraq, mainly due to high rainfall variance. The MK test revealed a reduction in 6- and 12-month droughts in the northwest and an intensification at a few northeastern grids. It indicates droughts became more recurrent in the already drought-prone region and lessened in a less drought-prone region.

A Distinctive Suite of Performance Metrics for Software Design

Shahid Iqbal,Muhammad Khalid,M.N.A. Khan 보안공학연구지원센터 2013 International Journal of Software Engineering and Vol.7 No.5

Enhanced Magnetic Moment of Epitaxial Υ´-Fe₄N Films at Low Temperature

Shahid Atiq,Murtaza Saleem,Shahid M. Ramay,Saadat A. Siddiqi,M. Sabieh Anwar,신성철 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.60 No.10

Ferromagnetic iron nitride has fascinated the researchers owing to its amazing magnetic properties for spintronic applications. We have prepared single phase epitaxial thin films of Υ´-Fe₄N on single crystal MgO(100) substrates using dc magnetron sputtering. X-ray diffraction revealed that substrate temperature and annealing time were most important parameters for single phase epitaxial growth of the films. Atomic force microscopy and scanning electron microscopy were utilized to investigate the surface morphology of the films. Angle dependent magnetic anisotropy was observed in epitaxial thin films of Υ´-Fe₄N. A superconducting interference device was utilized to explore the magnetic and electrical behavior of Υ´-Fe₄N films at low temperatures.

Antibacterial potential of the extracts derived from leaves and in vitro raised calli of medicinal plants Pterocarpus marsupium Roxb., Clitoria ternatea L., and Sanseveiria cylindrica Bojer ex Hook

Shahid, M,Shahzad, A,Anis, M Kyung Hee Oriental Medicine Research Center 2009 Oriental pharmacy and experimental medicine Vol.9 No.2

Pterocarpus marsupium, Clitoria ternatea, and Sanseveiria cylindrica are some of the important and endangered medicinal plant species of India. Despite of medicinal properties, antibacterial potential of the plants have not yet been explored. The present study was designed to optimize the in vitro technique for micropropagation and to screen the extracts from leaves and in vitro raised calli for antibacterial properties. Excised leaf-explants from the parent plants were surface sterilized and cultivated on Murashige & Skoog's (MS) medium containing $N^6$-benzyladenine (BA) in concentrations of 1, 2, 5, and $10{\mu}M$. Optimal growth of calli was noticed at a concentration of $5{\mu}M$, therefore the extracts from calli grown at this concentration were further studied for antibacterial activity. Both alcoholic and aqueous extracts from leaves of respective plants, and their in vitro raised calli were tested for antibacterial activity by agar well diffusion method against a range of Gram-positive and Gram-negative bacteria. Aqueous extracts showed antibacterial activity against limited number of bacterial species; notably the extracts of C. ternatea which showed antibacterial activity against Streptococcus pyogenes, Bacillus subtilis and Bacillus cereus. Alcoholic extracts of all three plants showed antibacterial activity against a wider range of bacteria. Among the Gram-positive bacteria, extracts from C. ternatea showed strong antibacterial activity against Bacillus spp., whereas the extracts of S. cylindrica showed good antibacterial potential for Staphylococcus aureus, S. epidermidis and S. pyogenes. The extracts from all three plants showed antibacterial activity against Gram-negative bacteria, including, Salmonella spp. and Shigella dysenteriae; organisms causing enteric fever and dysentery. In most of the cases, the extracts from respective calli showed comparable, and in some cases better, result in comparison to the extracts from parent leaves. To the best of our knowledge this is the first preliminary report on antibacterial potential, especially through calli extracts, of these plants; and in vitro cultivation of the explants may be used to obtain phytotherapeutic compounds.

Expression and cDNA Cloning of klp-12 Gene Encoding an Ortholog of the Chicken Chromokinesin, Mediating Chromosome Segregation in Caenorhabditis elegans

Shahid S. Siddiqui,Ken Nishikawa,M.A.Shakir,M. Yusuf Ali,K.Fukami Kobayashi,M.L.A.Khan The Korea Science and Technology Center 2000 BMB Reports Vol.33 No.2

In eukaryotes, chromosomes undergo a series of complex and coordinated movements during cell division. The kinesin motor proteins, such as the chicken Chromokinesin, are known to bind DNA and transport chromosomes on spindle microtubles. We previously cloned a family of retrograde C-terminus kinesins in Caenorhabditis elegans that mediate chromosomal movement during embryonic development. Here we report the cloning of a C. elegans klp-12 cDNA, encoding an ortholog of chicken Chromokinesin and mouse KIF4. The KLP-12 protein contains 1609 amino acid and harbors two leucine zipper motifs. The in situ RNA hybridization in embryonic stages shows that the klp-12 gene is expressed during the entire embryonic development. The RNA interference assay reveals that, similar to the role of Chromokinesin, klp-12 functions in chromosome segregation. These results support the notion that during mitosis both types, the anterograde N-terminus kinesins such as KLP-12 and the retrograde C-terminus kinesins, such as KLP-3, KLP-15, KLP-16, and KLP-17, may coordinate chromosome assembly at the metaphase plate and chromosomal segregation towards the spindle poles in C. elegans.

Expression and cDNA Cloning of klp-12 Gene Encoding an Ortholog of the Chicken Chromokinesin , Mediating Chromosome Segregation in Caenorhabditis elegans

Ali, M . Yusuf,Khan, M . L . A .,Shakir, M . A .,Kobayashi, K . Fukami,Nishikawa, Ken,Siddiqui, Shahid S . 생화학분자생물학회 2001 BMB Reports Vol.33 No.2

In eukaryotes, chromosomes undergo a series of complex and coordinated movements during cell division. The kinesin motor proteins, such as the chicken Chromokinesin, are known to bind DNA and transport chromosomes on spindle microtubles. We previously cloned a family of retrograde C-terminus kinesins in Caenorhabditts elegans that mediate chromosomal movement during embryonic development. Here we report the cloning of a C. elegans klp-12 cDNA, encoding an ortholog of chicken Chromokinesin and mouse KIF4. The KLP-12 protein contains 1609 amino acid and harbors two leucine zipper motifs. The in situ RNA hybridization in embryonic stages shows that the klp-12 gene is expressed during the entire embryonic development. The RNA interference assay reveals that, similar to the role of Chromokinesin, klp-12 functions in chromosome segregation. These results support the notion that during mitosis both types, the anterograde N-terminus kinesins such as KLP-12 and the retrograde C- terminus kinesins, such as KLP-3, KLP-15, KLP-16, and KLP-17, may coordinate chromosome assembly at the metaphase plate and chromosomal segregation towards the spindle poles in C. elegans.

Expression and cDNA Cloning of klp-12 Gene Encoding an Ortholog of the Chicken Chromokinesin, Mediating Chromosome Segregation in Caenorhabditis elegans

Ali, M. Yusuf,Khan, M.L.A.,Shakir, M.A.,Kobayashi, K. Fukami,Nishikawa, Ken,Siddiqui, Shahid S. 생화학분자생물학회 2000 Journal of biochemistry and molecular biology Vol.33 No.2

In eukaryotes, chromosomes undergo a series of complex and coordinated movements during cell division. The kinesin motor proteins, such as the chicken Chromokinesin, are known to bind DNA and transport chromosomes on spindle microtubles. We previously cloned a family of retrograde C-terminus kinesins in Caenorhabditis elegans that mediate chromosomal movement during embryonic development. Here we report the cloning of a C. elegans klp-12 cDNA, encoding an ortholog of chicken Chromokinesin and mouse KIF4. The KLP-12 protein contains 1609 amino acid and harbors two leucine zipper motifs. The insitu RNA hybridization in embryonic stages shows that the klp-12 gene is expressed during the entire embryonic development. The RNA interference assay reveals that, similar to the role of Chromokinesin, klp-12 functions in chromosome segregation. These results support the notion that during mitosis both types, the anterograde N-terminus kinesins such as KLP-12 and the retrograde C-terminus kinesins, such as KLP-3, KLP-15, KLP-16, and KLP-17, may coordinate chromosome assembly at the metaphase plate and chromosomal segregation towards the spindle poles in C. elegans.

Enabling high-mobility, ambipolar charge-transport in a DPP-benzotriazole copolymer by side-chain engineering

Gruber, M.,Jung, S. H.,Schott, S.,Venkateshvaran, D.,Kronemeijer, A.,Andreasen, J.,McNeill, C.,Wong, W. H.,Shahid, M.,Heeney, M. THE ROYAL SOCIETY OF CHEMISTRY 2015 Chemical Science Vol.6 No.12