MoS2/Au-Sensitized TiO2 Nanotube Arrays with Core–Shell Nanostructure for Hydrogen Production

Xiaorong Cheng,Yuhua Lu,Shoulin Gu,Graham Dawson 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.9

Herein, a TiO2 NTAs-Au-MoS2 core–shell photoanode was constructed with the intention to fulfill the efficient transfer of photo-generated carriers to the photoelectrode's surface. Au nanoparticles were decorated by a drop casting method, and the MoS2 layer was deposited above the Au nanoparticles using a photoreduction-annealing process. Au nanoparticles were well dispersed on the inner wall of the TiO2 nanotubes and covered by the MoS2 layer, forming a core–shell nanostructure. The MoS2 layer significantly improved the attachment between Au nanoparticles and TiO2 NTAs, resulting in increased PEC stability and performance. Attributed to the excitation of Au nanoparticles' localized surface plasmon resonance effect and visible light utilization of MoS2, the TiO2 NTAs-Au-MoS2 core–shell photoanode exhibits greatly enhanced photocurrent density. An increase from 67 μA/cm2 to 234 μA/cm2 under Xe lamp illumination and from 2.6 μA/cm2 to 12.6 μA/cm2 under visible light illumination ( λ > 420 nm) compared with the TiO2 NTAs was observed.

Development and on-orbit operation of loop heat pipes on chinese circumlunar return and reentry spacecraft

Zhang Hongxing,Mi Min,Miao Jianyin,Wang Lu,Chen Yang,Ding Ting,Ning Xianwen,Huo Yuhua 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.6

This paper describes the development and result of ground experiment and analyzes the on-orbit operation of Loop heat pipes (LHP) for the Chinese circumlunar return and reentry spacecraft. A novel LHP was developed to satisfy the thermal control requirements of Inertial measurement units (IMU). The LHP consisted of an integrated evaporator subassembly, a cambered condenser, and an electric explosion valve. Ground experiments were conducted to investigate the startup characteristics, and heat transfer and heat insulation performance of the LHPs. During a flight mission, the LHPs work as a thermal switch and perform both heat transfer and insulation functions. Before returning to the earth’s atmosphere, the valves of the reentry capsule are opened to discharge working fluid and prevent heat transfer from the capsule wall to the devices. In the flight mission, the LHPs performed all the required functions, and the IMU temperatures were maintained within an allowable range.

Boosting photocharge separation in Z-schemed g-C3N4/RGO/ln2S3 photocatalyst for H2 evolution and antibiotic degradation

Bingrong Guo,Bin Liu,Chaoli Wang,Junhong Lu,Yuhua Wang,Shu Yin,Muhammad Sufyan Javed,Wei-Hua Han 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.110 No.-

Due to the high redox ability and effective separation of photocharges, Z-schemed photocatalysts havebeen extensively used to deal with energy crises and environmental pollution. Here, a novel Zschemedg-C3N4/RGO/ln2S3 photocatalyst was designed and prepared for hydrogen generation and tetracyclinehydrochloride degradation. The properties of optical absorption, separation, and transfer of photochargesand heterojunction on photocatalytic activity of g-C3N4/RGO/ln2S3 composite have beenstudied. The characterization results showed that the Z-scheme photocatalyst has high efficiency onthe separation and transfer of photocharges, and the strong redox ability has also remained. Thus, theg-C3N4/RGO/ln2S3 composite exhibited superior photocatalytic performance than pristine ln2S3 and g-C3N4 in the same situations. The H2 evolution rate can reach up to 512.72 lmolg-1h1 and the degradationof tetracycline hydrochloride can reach 95.6% in 60 min under visible light irradiation. This work providesa potential pathway for designing and preparing high-performance photocatalysts to produce cleanenergy and purify the environment.

CircBLNK regulates tumor proliferation and apoptosis by miR-578/ING5 axis in non-small cell lung cancer

Li Ping,Zou Liuyi,Luo Zuojun,Lu Yuhua,Yu Shuang,Zhu Yujun,Xie Yong 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.3

Background Non-small cell lung cancer (NSCLC) is one of most threatening malignancies with a high morbidity and mortality that threaten human health and life. Objective This study aimed to investigate the role of circBLNK in NSCLC and reveal the regulation mechanism of circBLNK in NSCLC. Quantitative real-time polymerase chain reaction ( qRT-PCR) was performed to determine the levels of circBLNK, miR-578 and inhibitor of growth 5 (ING5) mRNA. Cell proliferation activity was assessed by 3-(4,5-dimethyl-2-thiazolyl)- 2,5-diphenyl-2-H-tetrazolium bromide (MTT), 5-ethynyl-2’-deoxyuridine (EdU) staining and colony formation assays. Flow cytometry was carried out to examine cell cycle and cell apoptosis. The dual-luciferase reporter assay was used to validate the interaction between miR-578 and circBLNK or ING5. Xenograft tumor experiment was performed to uncover the function of circBLNK in vivo. Results CircBLNK was notably down-regulated in NSCLC tissues and cells. Overexpression of circBLNK suppressed the proliferation and accelerated the apoptosis of NSCLC cells in vitro . CircBLNK targeted miR-578, and circBLNK exerted its biological function in NSCLC cells through sponging miR-578. ING5 was verifi ed as a target of miR-578, and circBLNK increased the abundance of ING5 through targeting miR-578 in NSCLC cells. ING5 interference could partly reverse the biological eff ects of NSCLC cells mediated by circBLNK overexpression. CircBLNK overexpression repressed NSCLC tumor growth in vivo. Conclusion CircBLNK functioned as a tumor suppressor in NSCLC to suppress the proliferation and cell cycle and promote cell apoptosis of NSCLC cells through miR-578/ING5 axis.

High-Frequency Targeted Mutagenesis in Pseudomonas stutzeri Using a Vector-Free Allele-Exchange Protocol

( Ahmed E. Gomaa ),( Zhiping Deng ),( Zhimin Yang ),( Liguo Shang ),( Yuhua Zhan ),( Wei Lu ),( Min Lin ),( Yongliang Yan ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.2

The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and double crossover-recombination system using an optimized vector-free allele-exchange protocol for gene disruption and gene replacement in a single species of the family Pseudomonadaceae. The protocol is based on selfligation (circularization) for the DNA cassette which has been obtained by overlapping polymerase chain reaction (Fusion-PCR), and carries an antibiotic resistance cassette flanked by homologous internal regions of the target locus. To establish the reproducibility of the approach, three different chromosomal genes (ncRNA31, rpoN, rpoS) were knocked-out from the root-associative bacterium Pseudomonas stutzeri A1501. The results showed that the P. stutzeri A1501 mutants, which are free of any plasmid backbone, could be obtained via a single or double crossover recombination. In order to optimize this protocol, three key factors that were found to have great effect on the efficiency of the homologous recombination were further investigated. Moreover, the modified protocol does not require further cloning steps, and it enables the construction of multiple gene knock-in/out mutants sequentially. This work provides a simple and rapid mutagenesis strategy for genome editing in P. stutzeri, which may also be applicable for other gram-negative bacteria.

Biological characterization of mesenchymal stem cells from bovine umbilical cord

Hui Xiong,Wei Jun Guan,Yue Hui Ma,Chunyu Bai,Shuang Wu,Yuhua Gao,Taofeng Lu,Qingyun Hu 한국통합생물학회 2014 Animal cells and systems Vol.18 No.1

Mesenchymal stem cells (MSCs) are multi-potential cells that are able to proliferate and differentiate into othercell types. Much research has been done on the MSCs from the umbilical cord (UCMSCs) in human, mice, andavian, but little literature has been published about these cells in big livestock. Here, we choose Luxi cattle asthe experimental animal, we describe an external culture of the UCMSCs from it and summarize the biologicalcharacteristics of these cells, e.g., morphologic appearance, surface antigens, colony-forming ability, geneexpression, and differentiation potential were detected via using immunofluorescence and reverse transcriptionpolymerase chain reaction (RT-PCR). The induced cells, osteoblast, lipoblast, hepatocyte, islet cells, andneurocyte were identified by Alizarin red staining, Oil-red-O staining, Periodic acid-schiff staining, andDithizone staining and RT-PCR detection for specific genes. Results suggest that biological characteristics ofthe UCMSCs were similar to those of MSCs previously analyzed. The primary UCMSCs were sub-cultured topassage 32, the UCMSCs expressed gene CD29, CD44, CD73, CD90, and CD166, induced cells illustratedtypical staining, and expressed specific genes, which indicate that the UCMSCs could be a novel alternativesource of MSCs for experimental and clinical applications.