Research progress and treatment of radiation enteritis and gut microbiota

Huiwen Ren(Huiwen Ren),Qi Wu(Qi Wu),Zhiqiang Sun(Zhiqiang Sun),Mingming Fang(Mingming Fang),Jun Liu(Jun Liu),Judong Luo(Judong Luo) 대한방사선종양학회 2023 Radiation Oncology Journal Vol.41 No.2

Radiation enteritis is a kind of intestinal radiation injury in patients with pelvic and retroperitoneal malignancies after radiotherapy, and its occurrence and development process are very complicated. At present, studies have confirmed that intestinal microecological imbalance is an important factor in the formation of this disease. Abdominal radiation causes changes in the composition of the flora and a decrease in its diversity, which is mainly manifested by a decrease in beneficial bacterial species such as Lactobacilli and Bifidobacteria. Intestinal dysbacteriosis aggravates radiation enteritis, weakens the function of the intestinal epithelial barrier, and promotes the expression of inflammatory factors, thereby aggravating the occurrence of enteritis. Given the role of the microbiome in radiation enteritis, we suggest that the gut microbiota may be a potential biomarker for the disease. Treatment methods such as probiotics, antibiotics, and fecal microbiota transplantation are ways to correct the microbiota and may be an effective way to prevent and treat radiation enteritis. Based on a review of the relevant literature, this paper reviews the mechanism and treatment of intestinal microbes in radiation enteritis.

The effects of deletion of cellobiohydrolase genes on carbon source-dependent growth and enzymatic lignocellulose hydrolysis in Trichoderma reesei

Ren Meibin,Wang Yifan,Liu Guoxin,Zuo Bin,Zhang Yuancheng,Wang Yunhe,Liu Weifeng,Liu Xiangmei,Zhong Yaohua 한국미생물학회 2020 The journal of microbiology Vol.58 No.8

The saprophytic fungus Trichoderma reesei has long been used as a model to study microbial degradation of lignocellulosic biomass. The major cellulolytic enzymes of T. reesei are the cellobiohydrolases CBH1 and CBH2, which constitute more than 70% of total proteins secreted by the fungus. However, their physiological functions and effects on enzymatic hydrolysis of cellulose substrates are not sufficiently elucidated. Here, the cellobiohydrolase-encoding genes cbh1 and cbh2 were deleted, individually or combinatively, by using an auxotrophic marker-recycling technique in T. reesei. When cultured on media with different soluble carbon sources, all three deletion strains (Δcbh1, Δcbh2, and Δcbh1Δcbh2) exhibited no dramatic variation in morphological phenotypes, but their growth rates increased apparently when cultured on soluble cellulase-inducing carbon sources. In addition, Δcbh1 showed dramatically reduced growth and Δcbh1Δcbh2 could hardly grew on microcrystalline cellulose (MCC), whereas all strains grew equally on sodium carboxymethyl cellulose (CMC-Na), suggesting that the influence of the CBHs on growth was carbon source-dependent. Moreover, five representative cellulose substrates were used to analyse the influence of the absence of CBHs on saccharification efficiency. CBH1 deficiency significantly affected the enzymatic hydrolysis rates of various cellulose substrates, where acid pre-treated corn stover (PCS) was influenced the least. CBH2 deficiency reduced the hydrolysis of MCC, PCS, and acid pre-treated and delignified corncob but improved the hydrolysis ability of filter paper. These results demonstrate the specific contributions of CBHs to the hydrolysis of different types of biomass, which could facilitate the development of tailor-made strains with highly efficient hydrolysis enzymes for certain biomass types in the biofuel industry.

Analysis of Key Genes and Pathways Associated with Colorectal Cancer with Microarray Technology

Liu, Yan-Jun,Zhang, Shu,Hou, Kang,Li, Yun-Tao,Liu, Zhan,Ren, Hai-Liang,Luo, Dan,Li, Shi-Hong Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Objective: Microarray data were analyzed to explore key genes and their functions in progression of colorectal cancer (CRC). Methods: Two microarray data sets were downloaded from Gene Expression Omnibus (GEO) database and differentially expressed genes (DEGs) were identified using corresponding packages of R. Functional enrichment analysis was performed with DAVID tools to uncover their biological functions. Results: 631 and 590 DEGs were obtained from the two data sets, respectively. A total of 32 common DEGs were then screened out with the rank product method. The significantly enriched GO terms included inflammatory response, response to wounding and response to drugs. Two interleukin-related domains were revealed in the domain analysis. KEGG pathway enrichment analysis showed that the PPAR signaling pathway and the renin-angiotensin system were enriched in the DEGs. Conclusions: Our study to systemically characterize gene expression changes in CRC with microarray technology revealed changes in a range of key genes, pathways and function modules. Their utility in diagnosis and treatment now require exploration.

Design and Prediction of Three Dimensional Flows in a Low Speed Highly Loaded Axial Flow Fan

Liu, Xuejiao,Chen, Liu,Dai, Ren,Yang, Ailing Korean Society for Fluid machinery 2013 International journal of fluid machinery and syste Vol.6 No.2

This paper describes the design to increase the blade loading factor of a low speed axial flow fan from normal 0.42 to highly loaded 0.55. A three-dimensional viscous solver is used to model the flows in the highly-loaded and normal loaded stages over its operation range. At the design point operation the static pressure rise can be increased by 20 percent with a deficit of efficiency by 0.3 percent. In the highly loaded fan stage, the rotor hub flow stalls, and separation vortex extends over the rotor hub region. The backflow, which occurs along the stator hub-suction surface, changes the exit flow from the prescribed axial direction. Results in this paper confirm that the limitation of the two dimensional diffusion does not affect primarily on the fan's performance. Highly loaded fan may have actually better performance than its two dimensional design. Three dimensional designing approaches may lead to better highly loaded fan with controlled rotor hub stall.

ALMA Reveals Sequential High-mass Star Formation in the G9.62+0.19 Complex

Liu, Tie,Lacy, John,Li, Pak Shing,Wang, Ke,Qin, Sheng-Li,Zhang, Qizhou,Kim, Kee-Tae,Garay, Guido,Wu, Yuefang,Mardones, Diego,Zhu, Qingfeng,Tatematsu, Ken’ichi,Hirota, Tomoya,Ren, Zhiyuan,Liu, Sheng-Yu American Astronomical Society 2017 The Astrophysical journal Vol.849 No.1

<P>Stellar feedback from high-mass stars (e.g., H II regions) can strongly influence the surrounding interstellar medium and regulate star formation. Our new ALMA observations reveal sequential high-mass star formation taking place within one subvirial filamentary clump (the G9.62 clump) in the G9.62+0.19 complex. The 12 dense cores (MM1-MM12) detected by ALMA are at very different evolutionary stages, from the starless core phase to the UC H II region phase. Three dense cores (MM6, MM7/G, MM8/F) are associated with outflows. The mass-velocity diagrams of the outflows associated with MM7/G and MM8/F can be well-fit by broken power laws. The mass-velocity diagram of the SiO outflow associated with MM8/F breaks much earlier than other outflow tracers (e.g., CO, SO, CS, HCN), suggesting that SiO traces newly shocked gas, while the other molecular lines (e.g., CO, SO, CS, HCN) mainly trace the ambient gas continuously entrained by outflow jets. Five cores (MM1, MM3, MM5, MM9, MM10) are massive starless core candidates whose masses are estimated to be larger than 25 M-circle dot, assuming a dust temperature of <= 20 K. The shocks from the expanding H II regions ('B' and 'C') to the west may have a great impact on the G9.62 clump by compressing it into a filament and inducing core collapse successively, leading to sequential star formation. Our findings suggest that stellar feedback from H II regions may enhance the star formation efficiency and suppress low-mass star formation in adjacent pre-existing massive clumps.</P>

5-Aza-2'-deoxycytidine Induces Hepatoma Cell Apoptosis via Enhancing Methionine Adenosyltransferase 1A Expression and Inducing S-Adenosylmethionine Production

Liu, Wei-Jun,Ren, Jian-Guo,Li, Ting,Yu, Guo-Zheng,Zhang, Jin,Li, Chang-Sheng,Liu, Zhi-Su,Liu, Quan-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

In hepatocellular cancer (HCC), lack of response to chemotherapy and radiation treatment can be caused by a loss of epigenetic modifications of cancer cells. Methionine adenosyltransferase 1A is inactivated in HCC and may be stimulated by an epigenetic change involving promoter hypermethylation. Therefore, drugs releasing epigenetic repression have been proposed to reverse this process. We studied the effect of the demethylating reagent 5-aza-2'-deoxycitidine (5-Aza-CdR) on MAT1A gene expression, DNA methylation and S-adenosylmethionine (SAMe) production in the HCC cell line Huh7. We found that MAT1A mRNA and protein expression were activated in Huh7 cells with the treatment of 5-Aza-CdR; the status of promoter hypermethylation was reversed. At the same time, MAT2A mRNA and protein expression was significantly reduced in Huh7 cells treated with 5-Aza-CdR, while SAMe production was significantly induced. However, 5-Aza-CdR showed no effects on MAT2A methylation. Furthermore, 5-Aza-CdR inhibited the growth of Huh7 cells and induced apoptosis and through down-regulation of Bcl-2, up-regulation of Bax and caspase-3. Our observations suggest that 5-Aza-CdR exerts its anti-tumor effects in Huh7 cells through an epigenetic change involving increased expression of the methionine adenosyltransferase 1A gene and induction of S-adenosylmethionine production.

Application of amine-loaded activated carbon fiber in CO2 capture and separation

Haoran Liu,Xinmei Lu,Liying Liu,Jian Wang,Pengyu Wang,Peng Gao,Tingsheng Ren,Guo Tian,Di Wang 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.9

The CO2 emitted by coal-fired power plants is the main factor leading to global warming, and the captureof CO2 in the flue gas of power plants is still the main task at this stage. Many adsorbents have been developed to captureCO2 in high-temperature flue gas, but some materials are complicated to synthesize or the cost is too high. Here,we used low-cost raw materials activated carbon fiber and PEI, and used green synthesis to synthesize new adsorbentsin order to capture CO2 in high-temperature flue gas of a power plant. To improve the performance of highly porousactivated carbon fiber (ACF) in CO2 capture and separation, an organic polymer polyethylenimine (PEI) was loadedsuccessfully into the oxidized ACF. The modified adsorbent was tested by FT-IR, XRD and SEM, and the CO2 adsorptioncapacity and CO2/N2 selectivity were analyzed. The results showed that the as-synthesized PEI-modified adsorbenthas a CO2 adsorption capacity of 2.5mmol/g, which is 1.7 times better than that of the pristine ACF adsorbent(1.5mmol/g), at 1 bar and 333 K, and it has excellent CO2/N2 selectivity, as calculated by ideal adsorption solution theory(IAST). These data indicate that PEI was loaded successfully into the oxidized ACF. In addition, the dual site Langmuirisotherm equation and Langmuir isotherm equation can be in good agreement with the adsorption curves ofCO2 and N2. In comparison with other composite adsorbents, the preparation process of the present new adsorbent ishighly environmentally friendly, the synthesis method is simple and the cost is low, which demonstrates potential applicationsin the separation of CO2 from the flue gas of power plants.

Copolymerizations of Ethylene with 1-Hexene over MgCl2/SiO2 Bi-Supported Titanium Catalysts: Effect of SiO2 on Active Site Distribution

Hegang Ren,김일,Min Yang,Yujin Liu,Binyuan Liu,Jiayan Qu,Yanji Wang 한국고분자학회 2012 Macromolecular Research Vol.20 No.2

Methanol synthesis from CO2 hydrogenation over Cu/g-Al2O3 catalysts modified by ZnO, ZrO2 and MgO

Hong Ren,Cheng-Hua Xu,Hao-Yang Zhao,Ya-Xue Wang,Jie Liu,Jian-Ying Liu 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.28 No.-

Cu/g-Al2O3 catalysts for methanol synthesis from CO2 are prepared and modified by metal oxides viaimpregnation in the present work. Results indicate that promoters modification leads to the formation ofsmall Cu0 particles with a high dispersion, improves catalytic performance of Cu-based catalysts inmethanol synthesis. Moreover, the activation temperature is another important factor on affecting theCu0 dispersion and particle size. The investigation on CO2 hydrogenation shows that methanol is mainlyfrom hydrogenation of activated CO2 with active hydrogen on Cu0 particles, which is inhibited by highreaction temperature due to improvement on reverse water-gas shift reaction and methanation

Isospecific Polymerizations of 1-Butene Catalyzed by MgCl2/TiCl4/Internal Donor-AlR3/External Donor System

Hegang Ren,김일,Min Yang,Baojun Zhang,Xiaofei Ren,Binyuan Liu,Yanji Wang 한국고분자학회 2012 Macromolecular Research Vol.20 No.9

1-Butene polymerizations were carried out by using a MgCl2/TiCl4/ID-AlR3/ED catalyst system in n-hexane,therein the internal donor (ID) is diisobutyl phthalate (DIBP), the external donor (ED) is diphenyl dimethoxysilane (DDS) or cyclohexyl methyl dimethoxysilane (CHMMS), and the AlR3 activator is AlEt3 or Al(i-Bu)3. The effect of the molar ratio of DIBP/Mg and polymerization conditions on the catalytic activity and the isotactic index (II) of poly(1-butene) (PB) was investigated. The dosage of DIBP remarkably improved the catalytic activity and the II of PB. CHMMS was more effective than DDS for increasing the II of PB. The addition of a small amount of hydrogen enhanced the catalytic activity from 15.1 to 17.5 kg-PB·gTi-1 and the II value of PB from 89.3% to 95.1%. The mixture of AlR3 was beneficial to increase the catalytic activity with negligible effect on the isospecificity.