http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Liu Xiangmei (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
Ren Meibin,Wang Yifan,Liu Guoxin,Zuo Bin,Zhang Yuancheng,Wang Yunhe,Liu Weifeng,Liu Xiangmei,Zhong Yaohua 한국미생물학회 2020 The journal of microbiology Vol.58 No.8
The saprophytic fungus Trichoderma reesei has long been used as a model to study microbial degradation of lignocellulosic biomass. The major cellulolytic enzymes of T. reesei are the cellobiohydrolases CBH1 and CBH2, which constitute more than 70% of total proteins secreted by the fungus. However, their physiological functions and effects on enzymatic hydrolysis of cellulose substrates are not sufficiently elucidated. Here, the cellobiohydrolase-encoding genes cbh1 and cbh2 were deleted, individually or combinatively, by using an auxotrophic marker-recycling technique in T. reesei. When cultured on media with different soluble carbon sources, all three deletion strains (Δcbh1, Δcbh2, and Δcbh1Δcbh2) exhibited no dramatic variation in morphological phenotypes, but their growth rates increased apparently when cultured on soluble cellulase-inducing carbon sources. In addition, Δcbh1 showed dramatically reduced growth and Δcbh1Δcbh2 could hardly grew on microcrystalline cellulose (MCC), whereas all strains grew equally on sodium carboxymethyl cellulose (CMC-Na), suggesting that the influence of the CBHs on growth was carbon source-dependent. Moreover, five representative cellulose substrates were used to analyse the influence of the absence of CBHs on saccharification efficiency. CBH1 deficiency significantly affected the enzymatic hydrolysis rates of various cellulose substrates, where acid pre-treated corn stover (PCS) was influenced the least. CBH2 deficiency reduced the hydrolysis of MCC, PCS, and acid pre-treated and delignified corncob but improved the hydrolysis ability of filter paper. These results demonstrate the specific contributions of CBHs to the hydrolysis of different types of biomass, which could facilitate the development of tailor-made strains with highly efficient hydrolysis enzymes for certain biomass types in the biofuel industry.
-
Changrong Liu,Hongbin Tan,Aiguo Zheng,Xiangmei Kang,Ao Jiang,Rui Fang,Haorong Ren,Wanwei Fang 한양대학교 세라믹연구소 2020 Journal of Ceramic Processing Research Vol.21 No.3
The manufacture of sodium sulfide through a carbon reduction process, using sodium sulfate as raw material, generatessodium sulfide by-product sediment, which has potential health and environmental impacts. Herein, a novel strategy isproposed to recover sodium oxide from the sediment by using acidic treatment and the influence of solution pH on sodiumoxide content is systematically studied. The results reveal that the sodium oxide content decreases with decreasing pH valueof the solution. At pH = 4, the as-treated sediment results in Na2O content of 3.10 wt. %, which recovery rate is about 90%. Furthermore, the influences of sintering temperature and time on compressive strength and bulk density are studied. Ingeneral, the compressive strength and bulk density increase with increasing sintering temperature and time. After sinteringat 1,300 oC for 120 min, the compressive strength and bulk density of the sintered porous ceramic are 26.66 MPa and 1.31 g/cm3, respectively. The porous ceramic, sintered at 1,300 oC, mainly consists of hauyne, gehlenite and hematite phases. Insummary, the few flaws in cell-walls result in high compressive strength of the as-prepared porous ceramics.
-
Yang Lu,Shuxin Liu,Shujia Zhang,Guangyan Cai,Hongwei Jiang,Huabin Su,Xiaofan Li,Quan Hong,Xueguang Zhang,Xiangmei Chen 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.3
Tissue inhibitor of metalloproteinase-1 (TIMP-1) plays various roles in cell growth in different cell types. However, few studies have focused on TIMP-1’s effect on fibroblast cells. In this study, we investigated the effects of TIMP-1 overexpression on NIH3T3 fibroblast proliferation and potential transduction signaling pathways involved. Overexpression of TIMP-1, by transfection of the pLenti6/ V5-DESTTIMP-1 plasmid, significantly promoted NIH3T3 proliferation as determined by the BrdU array. Neither 5 nor 15 nM GM6001 (matrix metalloproteinase system inhibitor) affected NIH3T3 proliferation, but 45 nM GM6001 inhibited proliferation. TIMP-1 overexpression activated the p-Akt pathway, but not the p-ERK or p-p38 pathway. In TIMP-1-transfected cells, cyclinD1 was upregulated and p21CIP1 and p27^(KIP1) were downregulated, which promoted cell entry into the S and G2/M phases. The PI3-K inhibitor LY294002 abolished the TIMP-1-induced effects. Overexpression of intracellular TIMP-1 stimulated NIH3T3 fibroblast proliferation in a matrix metalloproteinase (MMP)-independent manner by activating the p-Akt pathway and related cell cycle progression.
-
Li Zhao,Jizhou Lv,Fei Li,Kairui Li,Bo He,Luyao Zhang,Xueqing Han,Huiyu Wang,Nicholas Johnson,Xiangmei Lin,Shaoqiang Wu,Yonghong Liu 대한기생충학ㆍ열대의학회 2020 The Korean Journal of Parasitology Vol.58 No.1
Livestock husbandry is vital to economy of the Tarim Basin, Xinjiang Autonomous Region, China. However, there have been few surveys of the distribution of ixodid ticks (Acari: Ixodidae) and tick-borne pathogens affecting domestic animals at these locations. In this study, 3,916 adult ixodid ticks infesting domestic animals were collected from 23 sampling sites during 2012-2016. Ticks were identified to species based on morphology, and the identification was confirmed based on mitochondrial 16S and 12S rRNA sequences. Ten tick species belonging to 4 genera were identified, including Rhipicephalus turanicus, Hyalomma anatolicum, Rh. bursa, H. asiaticum asiaticum, and Rh. sanguineus. DNA sequences of Rickettsia spp. (spotted fever group) and Anaplasma spp. were detected in these ticks. Phylogenetic analyses revealed possible existence of undescribed Babesia spp. and Borrelia spp. This study illustrates potential threat to domestic animals and humans from tick-borne pathogens.
DBpia연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
