Gene Expression Analysis of the Bromobenzene Treated Liver with Non-hepatotoxic Doses in Mice

Lim, Jung-Sun,Jeong, Sun-Young,Hwang, Ji-Yoon,Park, Han-Jin,Cho, Jae-Woo,Song, Chang-Woo,Kim, Yang-Seok,Lee, Wan-Seon,Moon, Jin-Hee,Han, Sang-Seop,Yoon, Seok-Joo The Korean Society of Toxicogenomics and Toxicopro 2005 Molecular & cellular toxicology Vol.1 No.4

Bromobenzene (BB) is well known hepatotoxicant. Also, BB is an industrial solvent that arouses toxicity predominantly in the liver where it causes centrilobular necrosis. BB is subjected to Cytochrome P450 mediated epoxidation followed by either conjugation with glutathione, enzymatic hydrolysis or further oxidation. In this study, we focused on BB-induced gene expression at non-hepatotoxic dose. Mice were exposed to two levels of BB, sampled at 24 h, and hepatic gene expression levels were determined to evaluate dose dependent changes. When examining the toxic dose of BB treated group in other previous studies, genes related to heat shock protein, oxidative stress, and drug metabolism are expressed. Compared to these results, our study, in which non-toxic dose of BB was administrated, showed similar patterns as the toxic conditions above. The purpose of the study was to select genes that showed changes in relation to the differing dose through confirmation of the difference within transcriptomic boundaries, but those that are not detected by the existing classic toxicology tools in non-hepatotoxic dose.

Interfacial Trap-based 1-row Hammer Analysis of BCAT and Nitride Layer BCAT Structures in Dynamic Random Access Memory

Chang Young Lim,Yeon-Seok Kim,Min-Woo Kwon 대한전자공학회 2024 Journal of semiconductor technology and science Vol.24 No.1

Dynamic Random Access Memory is critical to computing for its speed and cost-effective capacity. As the demand for high-capacity memory grows, DRAM is being scaled down. However, reduced cell distances cause electrical disturbances between cells, resulting in 1-row hammer. This leads to abnormal operation and security risks. Therefore, 1-row Hammer is a major issue in modern DRAM technology. In this paper, we study the principle and impact of 1-row Hammer in DRAM, with a focus on D0 failures, a type of 1-row Hammer that causes stored data to transition from 0 to 1 due to repeated access. The mechanism involves the electron capture and diffusion of electrons affected by interfacial traps and device structures. To investigate the D0 failure, we reproduced the 1-row hammer using mixed mode to evaluate the effects on the interfacial trap and device structure changes. This research serves to improve understanding of row hammer and suggests a mitigation strategy using nitride layer. The proposed structure improves the D0 failure by about 70%, effectively improving the security vulnerability of DRAM.

Allogenic succession of Korean fir (<i>Abies koreana Wils.</i>) forests in different climate condition

Lim, Chi Hong,An, Ji Hong,Jung, Song Hie,Lee, Chang Seok Springer Japan 2018 Ecological research Vol.33 No.2

<P><B>Abstract</B></P><P>This study was conducted to clarify the changes in vegetation that occurred due to changing environmental factors, especially climate, at Korean fir (<I>Abies koreana</I>) stands with different climatic conditions established on Mt. Halla, which is located on a southern island of South Korea. The difference of species composition between sites was large and depended on elevation and slope aspect at lower elevations, whereas not as much among stands or between sites at the highest elevations of each slope aspect. It was interpreted that differences and similarities among sites were dominated by the microclimate determined by the topographic conditions of each site. The result of vegetation dynamics analysis predicted that the Korean fir forests would be replaced by temperate forests such as Mongolian oak (<I>Quercus mongolica</I>) forests or shade intolerant forests composed of early successional species such as Korean cherry (<I>Prunus maximowiczii</I>) and Spreading yew (<I>Taxus cuspidata</I>) at lower elevations, while would continuously persist at the highest elevations. We interpreted the vegetation changes appeared at the lower elevations as an allogenic succession, as the recent rapid climate changes directly and indirectly dominated the change. The species distribution modeling predicted that the distributional range of Korean fir would decrease to 13.4 and 10.1% of the current distribution in 2050 and 2070, respectively. Further, the distribution modeling showed that the sites located at lower elevations would no longer be within the distributional range of Korean fir forest, and those at the highest elevations would be sparsely scattered in fragmented states.</P>

Age-Associated Changes in the Vascular Renin-Angiotensin System in Mice

Yoon, Hye Eun,Kim, Eun Nim,Kim, Min Young,Lim, Ji Hee,Jang, In-Ae,Ban, Tae Hyun,Shin, Seok Joon,Park, Cheol Whee,Chang, Yoon Sik,Choi, Bum Soon Hindawi Publishing Corporation 2016 Oxidative medicine and cellular longevity Vol.2016 No.-

<P><I>Background</I>. This study evaluated whether the change in the renin-angiotensin system (RAS) is associated with arterial aging in mice.<I> Methods</I>. Histologic changes and expressions of transforming growth factor-<I>β</I> (TGF-<I>β</I>), collagen IV,<I> fibronectin</I>, angiotensin II (Ang II), angiotensin-converting enzyme (ACE), angiotensin-converting enzyme 2 (ACE2), angiotensin II type 1 receptor (AT1R), angiotensin II type 2 receptor (AT2R), prorenin receptor (PRR), Mas receptor (MasR), endothelial nitric oxide synthase (eNOS), NADPH oxidase 2 and oxidase 4 (Nox2 and Nox4), 8-hydroxy-2′-deoxyguanosine (8-OHdG),<I> 3-nitrotyrosine</I>, and superoxide dismutase 1 and dismutase 2 (SOD1 and SOD2) were measured in the thoracic aortas from 2-month-old, 12-month-old, and 24-month-old C57/BL6 mice.<I> Results</I>. Twenty-four-month-old mice showed significantly increased aortic media thickness and expressions of TGF-<I>β</I>, collagen IV, and fibronectin, compared to 2-month-old and 12-month-old mice. The expressions of PRR, ACE, and Ang II, and AT1R-positive area significantly increased, whereas expressions of ACE2 and MasR and AT2R-positive area decreased with age. The expressions of phosphorylated serine<SUP>1177</SUP>-eNOS, SOD1, and SOD2 decreased, and the 8-OHdG-positive area and the 3-nitrotyrosine-positive area increased with age. The expression of Nox2 significantly increased with age, but that of Nox4 did not change.<I> Conclusions</I>. The enhanced PRR-ACE-Ang II-AT1R axis and reduced ACE2-MasR axis were associated with arterial aging in mice. </P>

폐질환이 동맥혈 이산화탄소 분압과 호기말 이산화탄소 분압의 차이에 미치는 영향

김재환,장문석,임춘학,박영철 대한마취과학회 1999 Korean Journal of Anesthesiology Vol.36 No.5

Background : The purpose of this study was to evaluate the effects of lung disease on the difference between arterial and end-tidal carbon dioxide tension by placing patients from the supine to the lateral decubitus position and by the changes from two lung ventilation (TLV) to one lung ventilation (OLV) during thoracic surgery. Method : Fifteen patients who had no parenchymal lung disease were selected for control group and 15 patients who had parenchymal lung disease on non-dependent lung were selected for disease group. All patients had been intubated with double lumen endobronchial tubes and respiration was controlled with a rate of 14∼15 breaths per minute and tidal volume 8 ml/kg. End-tidal and arterial carbon dioxide tension were measured at three different measurement periods (supine plus TLV, lateral decubitus plus TLV, lateral decubitus plus OLV). Results : The arterial to end-tidal carbon dioxide tension difference was more increased in disease group than control group. But there was no significnt difference in arterial to end-tidal carbon dioxide tension with position change, ventilation method change in each groups. Conclusion : We conclude that the arterial to end-tidal carbon dioxide tension difference is increased in lung disease, but it does not changed with position and ventilation method change. (Korean J Anesthesiol 1999; 36: 818∼822)

수술 전 이하선 종괴의 위치파악에 이용하는 해부학적 경계표의 유용도

임치영(Chi Young Lim),김국진(Kook Jin Kim),임성주(Sung Ju Lim),이잔디(Jan dee Lee),남기현(Kee Hyun Nam),장항석(Hang Seok Chang),정웅윤(Woong Youn Chung),최홍식(Hong Shik Choi),박정수(Cheong Soo Park) 대한두경부종양학회 2006 대한두경부 종양학회지 Vol.22 No.1

Background: The location of parotid gland tumors can influence the duration and the difficulty of the operation. If the information about tumor location was available preoperatively, it would allow accurate operative planning and counseling of patients in terms of the length of the operation and the potential morbidity. Methods: This study was based on a retrospective review of 100 patients with parotid gland tumors underwent parotidectomy from January 2000 to October 2005 at Yong-Dong Severance Hospital. Based on computed tomographic(CT) scan findings, 4 landmarks such as facial nerve(FN) line, Utrecht(U) line, Conn's are(CA), and retromandibular vein (RV) were drawn on the scans in same plane. The location of tumors were determined by the landmarks and confirmed by the operative findings. The accuracy of each landmarks was evaluated. To find out the accuracies according to tumor size, the tumors were divided into 2 groups; less than 2 cm and larger than 2 cm in diameter. Results: U line was the most accurate(94%), sensitive(89.3%) and specific(97.7%) in predicting tumor location of the parotid gland. However, in small tumors less than 2cm, FN line (p=0.022) and RV criteria (p=0.028) were more reliable in accuracy. Conclusion: CA, FN line, U line, and RV are all useful landmarks in preoperative prediction for the location of parotid gland tumors. However, U line was the most accurate, but we must consider that proper landmark should be used in prediction according to the size of tumor because the accuracy of landmark may change.

PET/CT 검사에서 환자체형에 따른 적정검사 프로토콜에 관한 고찰

조석원,함준철,강천구,반영각,이승재,임한상,이창호,박훈희,Cho, Seok Won,Ham, Joon-Chul,Kang, Chun Goo,Bahn, Young Kag,Lee, Seung Jae,Lim, Han Sang,Lee, Chang-Ho,Park, Hoon-Hee 대한핵의학기술학회 2013 핵의학 기술 Vol.17 No.2

Purpose: Whole-body PET using radiopharmaceutical is one of the imaging study methods for physiological changes of body. High specificity of the PET-CT examination is used to detect an early stages of cancer and metastatic cancer by imaging a physiological changes. During the imaging process, PET image has been characterized by a relatively low image quality due to its low sensitivity and the acquisition of random and scatter coincidences as well as patients figure. Therefore, the image quality as the changes of the acquisition times of patient weight was evaluated in this study. Materials and Methods: Thirty patients who presented to our hospital were enrolled. They were divided to normal, overweight, and obese group using BMI index, respectively. The patients with a liver disease and diabetes were excluded. $^{18}F-FDG$ was administered to the patients as 5.2 MBq per kg. After an hour from an injection, image acquisition was obtained as List mode in a part of liver in 1 bed. SNR (signal-to-noise ratio) of each groups acquisition times were confirmed from the calculated radiation counts and random fractions. The statistical significance of three groups was confirmed through one-way ANOVA test. On the basis of the counts of 2 minutes on normal group, the SNR of overweight group and obese group were compared. Results: The SNR were increased with loger aquisition time in 3 groups. In the condition of same acquisition time, the SNR had a statistical significance (P<0.05). The SNR were decreased to the normal, overweight, and obese, respectively. Liver activity had no significance difference on each group and RF had the significance differences (P<0.05). On the basis of the counts of 2 minutes on normal group, there were no statistical significance in a three minute acquisitions of overweight group and two minute acquisitions of obese group (P=0.150). Conclusion: In this study, the administrated amount of radiation dose did not adjust as the change of the patients weight. Increasing the acquisition time when the administration of the same amount of dose was able to get a good result of SNR. When the Based 2 minute on normal group, if overweight and obese case the increased acquisition time of 3 minute was able to obtain a similar SNR. On the basis of the normal group, the acquisition times of overweight and obese group were increased to 3 minutes per bed and the SNR were similar to the normal group.

자동차의 비틀림 강성 향상에 관한 연구

임기창,임석현 (社團法人)韓國火災·消防學會 1997 한국화재소방학회논문지 Vol.11 No.1

소방자동차를 비롯한 모든 자동차의 비틀림 강성이 약하면 차체에 크랙이 발생함과 동시에 고유진동수가 낮아져 진동소음 발생의 원인이 된다. 이 논문은 최소한의 중량증가로 비틀림 강성을 최대한 증가시키는 방안을 제시한 것에 관한 것으로, 방안검토를 위하여 탄성론을 이용한 이론해석을 실시하였고, 또한 이의 검증을 위하여 유한요소법에 의한 해석 및 실제차체를 이용한 실험을 실시하였다. 본 논문에서 사용한 자동차는 승용차를 사용하였다. 그 이유는 실험 및 계산이 용이하고, 또한 계산결과를 소방자동차 등을 비롯한 모든 자동차에 적용 할 수 있기 때문이다. Using high tensile steel plate makes the vehicle body stiffness lower even though it can lessen the fuel consume rate in application of weight iedosion. The crack which happens around vehicle window glass is brought about due to fatigue with low torsional stiffness. The paper presents a most suitable way to increase torsional stiffness using elasticity theory. Also the result of this study shows good agreement with FEM and experiments. We used a passenger car for calculation in this paper. Because we can apply the result of this study to fire engine as well as passenger car.

공간정보를 이용한 홍수재해지도 예측분석

임희창, 최석근 충북대학교 건설기술연구소 2013 충북대학교 건설기술연구소 논문집 Vol.32 No.1

본문참고

염색슬러지 탄화물 재활용 및 자원화 순환망 구축 타당성에 관한 연구

임창호,김주청,이동석 江原大學校 産業技術硏究所 2009 産業技術硏究 Vol.29 No.B

The dyeing sludge is the major waste generated in Banwol & Sihwa Industrial Complex. The Purpose of this study is to develop BAT (Best available technique) for the treatment and recycling of dyeing sludge. The dyeing sludge was carbonized and the product was tested physical properties and chemical chracterizations and also analyzed chemical compositions. From the test results we expect that the products can be used as adsorbent for the removal of order in the poultry farm and cattle shed. The feasibility of other methods such as manufature of RDF and cement etc. were also studied.