Research on User Clustering Collaborative Filtering Algorithm

Lihua Tian,Liguo Han,Junhua Yue 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.4

Memory-based CF algorithms have the weakness of low real-time ability and scalability. For these issues, a SVD-based K-means clustering CF algorithm is proposed. Traditional clustering-based CF algorithms have low recommendation precision because of data sparsity. So we first fill the missing ratings by SVD prediction, and then implement k-means clustering in the filled matix. This algorithm overcomse the data sparsity issue via SVD and keep the advantage of clustering, such as good real-time ability and scalability. Experiments results show that this algorithm outperforms Pearson CF, svd CF and k-means CF.

Research on Image Segmentation based on Clustering Algorithm

Lihua Tian,Liguo Han,Junhua Yue 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.2

Hierarchical clustering (HC) algorithm can obtain good clustering results, but it needs large storage and computational complexity for large image processing. Anew color image segmentation algorithm based on mean shift and hierarchical clustering algorithm named MSHC is presented in this paper. MSHC algorithm preprocesses an input image by MS algorithm to form segmented regions that preserve the desirable discontinuity characteristics of image. The number of segmented regions, instead of the number of image pixels, is considered as the input data scale of HC algorithm. The proximity between each cluster is calculated to form the proximity matrix, and then ward algorithm is employed to obtain the final segmentation results. MSHC algorithm is employed on color image and medical image segmentation.

Probiotics Increase Intramuscular Fat and Improve the Composition of Fatty Acids in Sunit Sheep through the Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Signaling Pathway

Yue Zhang,Duo Yao,Huan Huang,Min Zhang,Lina Sun,Lin Su,LiHua Zhao,Yueying Guo,Ye Jin 한국축산식품학회 2023 한국축산식품학회지 Vol.43 No.5

This experiment aims to investigate the impact of probiotic feed on growth performance, carcass traits, plasma lipid biochemical parameters, intramuscular fat and triglyceride content, fatty acid composition, mRNA expression levels of genes related to lipid metabolism, and the activity of the enzyme in Sunit sheep. In this experiment, 12 of 96 randomly selected Sunit sheep were assigned to receive the basic diet or the basic diet supplemented with probiotics. The results showed that supplementation with probiotics significantly increased the loin eye area, and decreased plasma triglycerides and free fatty acids, increasing the content of intramuscular fat and triglycerides in the muscle and improving the composition of the fatty acids. The inclusion of probiotics in the diet reduced the expression of adenosine 5’-monophosphate-activated protein kinase alpha 2 (AMPKα2) mRNA and carnitine palmitoyltransferase 1B (CPT1B) mRNA, while increasing the expression of acetyl-CoA carboxylase alpha (ACCα) mRNA, sterol regulatory elementbinding protein-1c (SREBP-1c) mRNA, fatty acid synthase mRNA, and stearoyl-CoA desaturase 1 mRNA. The results of this study indicate that supplementation with probiotics can regulate fat deposition and improves the composition of fatty acids in Sunit sheep through the signaling pathways AMPK-ACC-CPT1B and AMPK-SREBP-1c. This regulatory mechanism leads to an increase in intramuscular fat content, a restructuring of muscle composition of the fatty acids, and an enhancement of the nutritional value of meat. These findings contribute to a better understanding of the food science of animal resources and provide valuable references for the production of meat of higher nutritional value.

Effects of Sperm Pretreatments and In vitro Culture Systems on Development of In vitro Fertilized Embryos Derived from Prepubertal Boer Goat Oocytes in China

Lv, Lihua,Yue, Wenbin,Liu, Wenzhong,Ren, Youshe,Li, Fuzhong,Lee, Kyung-Bon,Smith, George W. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.7

Use of oocytes from prepubertal animals for in vitro embryo production holds potential application for reducing generation intervals and increasing genetic progress through embryo transfer. The objective of these studies was to compare the effect of three sperm pretreatments (prior to in vitro fertilization) and seven embryo culture protocols on fertilization rate and (or) subsequent development of in vitro fertilized embryos derived from oocytes harvested from ovaries of 1-6 month old prepubertal Boer goats in China. Cleavage rates were highest for embryos fertilized with heparin-treated versus calcium ionophore- or caffeine-treated sperm. Similar rates of blastocyst development were observed using heparin- and ionophore-treated sperm, which were higher than obtained with caffeine-treated sperm. No differences in cleavage or blastocyst rates were observed following embryo culture in basal medias (synthetic oviductal fluid (SOF), Charles Rosenkrans 1 (CR1) or tissue culture medium-199 (TCM-199)) containing 10% fetal bovine serum (FBS). Cumulus or oviductal cell co-culture did not enhance cleavage or blastocyst rates relative to culture in SOF+10% FBS. Replacement of FBS in SOF medium with 0.3% BSA increased cleavage rates, but did not increase rates of blastocyst development. Sequential culture in SOF+0.3% BSA followed by SOF+10% FBS increased blastocyst yield versus continuous culture in SOF+10% FBS and tended to increase blastocyst yield versus continuous culture in SOF+0.3% BSA. These results demonstrate a pronounced effects of sperm pretreatments and in vitro embryo culture systems on rates of blastocyst development and provide a potential protocol (sperm pretreatment with heparin and sequential embryo culture in SOF+0.3% BSA followed by SOF+10% FBS) for generation of the significant numbers of in vitro produced blastocysts from oocytes of prepubertal Boer goats necessary for application of embryo transfer in rural regions of China for distribution of Boer goat genetics.

Genome-wide identification of auxin-responsive microRNAs in the poplar stem

Yang Lihua,Ping Tao,Lu Wenjin,Song Sangfa,Wang Jianli,Wang Qiao,Chai Guohua,Bai Yue,Chen Yan 한국유전학회 2023 Genes & Genomics Vol.45 No.8

Background Wood (secondary xylem) of forests is a material of great economic importance. Wood development is strictly controlled by both the phytohormone auxin and microRNAs (miRNAs). Currently, the regulatory mechanisms underlying wood formation by auxin-associated miRNAs remain unclear. Objective This report was designed to identify auxin-responsive miRNAs during wood formation. Methods Morphological observation of wood development in the poplar stems was performed under the treatment of different concentrations (0 mg/L, CK; 5 mg/L, Low; 10 mg/L, High) of indol-3-butyric acid (IBA). Using a small RNA sequencing strategy, the effect of IBA treatment on miRNAs expression was genome-widely analyzed. Results In this study, we found that wood development of poplar was promoted by low concentration of IBA treatment but inhibited by high concentration of IBA treatment. Stringent bioinformatic analysis led to identification of 118 known and 134 novel miRNAs candidates. Sixty-nine unique developmental-related miRNAs, corresponding to 269 target genes, exhibited specific expression patterns in response to auxin, as was consistent with the influence of auxin application on wood formation. Three novel miRNAs had the most number (≥ 9) of target genes, belonging to SPL, GRF and ARF gene families. The evolutionary relationships and tissue expression patterns of 41 SPL, GRF and ARF genes in poplar were thus analyzed. Of them, four representative members and corresponding miRNAs were confirmed using RT-qPCR. Conclusions Our results may be helpful for a better understanding of auxin-induced regulation of wood formation in tree species.

SH-Sim : A Flexible Simulation Platform for Hybrid Storage Systems

Puyuan Yang,Peiquan Jin,Lihua Yue 보안공학연구지원센터(IJGDC) 2014 International Journal of Grid and Distributed Comp Vol.7 No.3

A Survey on Phase Change Memory-Aware Cache Management

Kaimeng Chen,Peiquan Jin,Lihua Yue 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.1

PCM (phase change memory) has been regarded as one of the most promising NVRAM. Due to its excellent features including high access speed, good scalability, byte addressability, and low idle power, PCM is expected to be an alternative to DRAM or flash memory in the future. So far, many different kinds of architecture have been proposed to merge PCM into the memory hierarchy of computer systems. For PCM-based computer systems, considering that PCM has various properties differing with DRAM and conventional storage media, traditional cache management policies based on DRAM and disks have to be re-designed. In this paper, we present a survey on the cache management policies for PCM-based systems that employ PCM as main memory or as secondary storage. Specially, for different PCM-based architectures, we explain how PCM-aware cache management policies exploit PCM’s advantages and overcome PCM’s drawbacks like asymmetric read/write latencies.

A Multi-Granularity Grid-Based Graph Model for Indoor Space

Na Wang,Peiquan Jin,Yan Xiong,Lihua Yue 보안공학연구지원센터 2014 International Journal of Multimedia and Ubiquitous Vol.9 No.4

A Novel Sea-Land Segmentation Algorithm Based on Local Binary Patterns for Ship Detection

Yu Xia,Shouhong Wan,Peiquan Jin,Lihua Yue 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.3

Slide Session : OS-GAS-18 ; Gastroenterology : Bahd1: A Gatekeeper to Protect Us from Inflamma-tion-Associated Colon Disease?

( Huatuo Zhu ),( Xinyong Wan ),( Wenguo Chen ),( Chaohui Yu ),( Min Yue ),( Yining Dai ),( Lihua Chen ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: It was recently reported that epigenetics might play an essential role in IBD. Bromo adjacent homology domain 1 (BAHD1), which involved in category of epigenetics maintains homeostasis by promoting heterochromatin formation. Our study aimed to investigate the underlying mechanism of BAHD1 in gut inflammation including IBD, seeking a new therapeutic target for the inflammation-associated colon disease. Methods: Experimental colitis was induced in C57BL/6 mice by dextran sulfate sodium administration. To simulate the intestinal inflammation microenvironment for epithelial cells, Caco-2 cells were exposed to a mixture of LPS, TNF-a, IL-1ß, and IFN-. BAHD1 expression was detected by quantitative PCR(qPCR), western blot and immunohistochemistry in both UC patients and mice model. Small interfering RNA was used to knock down BAHD1 level(siBAHD1) in Caco-2 cells and associated cytokines(CKs) expression were detected by either qPCR or ELISA. Possible mechanism involving in- flammatory pathways activation were addressed by western blot. Results: Murine model of UC-like inflammation was successfully established. And we found that BAHD1 existed in the normal internal crypt and surface epithelial cells ubiquitously. Compared with control group, BAHD1 expression in colon tissue were significantly decreased in both UC patients and mice model. In the vitro model system, we found that the protein level of BAHD1 was decreased in the stimulated Caco-2 cells. In addition, consistent with mRNA level of associated CKs enhanced in the siBAHD1 group within stimulatory factors, the interference group secreted more IL-6 and MCP-1 contents in the culture supernatant. As for potential mechanisms of BAHD1 in colitis, increased expression of TNFR1 was found in Caco-2 cells pre-treated with siBAHD1 in gut inflammation model, accompanying with the activation of IKK/ NF-κB and JNK/AP-1 pathways. Conclusions: Collectively, those findings provide evidence that BAHD1 might act as an indispensable safeguard to keep intestine immunological homeostasis.