Pharmacokinetics, tissue distribution and excretion of peimisine in rats assessed by liquid chromatography-tandem mass spectrometry

Lihua Chen,Dongxun Li,Guosong Zhang,Wei Zhang,Lihua Zhang,Yongmei Guan,Weifeng Zhu,Hongning Liu 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

Peimisine, the common ingredient of ‘‘zhebeimu’’groups and ‘‘chuanbeimu’’ groups, is responsiblefor the expectorant and cough relieving effects. The aim ofthis study was to investigate the pharmacokinetics, tissuedistribution and excretion of peimisine in male and femaleSD (Sprague-Dawley) rats by a rapid and sensitive LC-MS/MS (liquid chromatography-tandem mass spectrometry)method used carbamazepine as the internal standard afteroral administration, carbamazepine was stated as an IS. The results showed that peimisine was slowly distributed,and eliminated from rat plasma and manifested lineardynamics in a dose range of 0.26–6.5 mg/kg. Tested byANOVA, there were gender differences in the pharmacokineticparameters of AUC0-t, AUC0-? among a singledose of 0.26, 1.3, 6.5 mg/kg (P\0.05). Drug blood andtissue levels in male rats were significantly higher than thefemale counterparts after oral administration, while boththe males and the females showed high drug levels inspleen, kidney, lung, liver and heart. On the other hand, thepeimisine levels that can be reached in uterus, ovary, testisand brain is low. The excretion study showed that littleadministered peimisine (\0.7 %) was recovered in themale and female bile. Approximately 13.46 and 15.05 %were recovered in female urine and feces, while 43.07 and7.49 % were recovered in male urine and feces, respectively,which indicated that the major elimination route ofmale rats was urine excretion. In addition, there was significantdifferences in total cumulative excretive ratio ofpeimisine in feces (P\0.05) and no significant differencesin the urine (P[0.05) at a dose of 1.3 mg/kg.

Profiling Total Viable Bacteria in a Hemodialysis Water Treatment System

( Lihua Chen ),( Xuan Zhu ),( Menglu Zhang ),( Yuxin Wang ),( Tianyu Lv ),( Shenghua Zhang ),( Xin Yu ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.5

Culture-dependent methods, such as heterotrophic plate counting (HPC), are usually applied to evaluate the bacteriological quality of hemodialysis water. However, these methods cannot detect the uncultured or viable but non-culturable (VBNC) bacteria, both of which may be quantitatively predominant throughout the hemodialysis water treatment system. Therefore, propidium monoazide (PMA)-qPCR associated with HPC was used together to profile the distribution of the total viable bacteria in such a system. Moreover, high-throughput sequencing of 16S rRNA gene amplicons was utilized to analyze the microbial community structure and diversity. The HPC results indicated that the total bacterial counts conformed to the standards, yet the bacteria amounts were abruptly enhanced after carbon filter treatment. Nevertheless, the bacterial counts detected by PMA-qPCR, with the highest levels of 2.14 × 107 copies/100 ml in softener water, were much higher than the corresponding HPC results, which demonstrated the occurrence of numerous uncultured or VBNC bacteria among the entire system before reverse osmosis (RO). In addition, the microbial community structure was very different and the diversity was enhanced after the carbon filter. Although the diversity was minimized after RO treatment, pathogens such as Escherichia could still be detected in the RO effluent. In general, both the amounts of bacteria and the complexity of microbial community in the hemodialysis water treatment system revealed by molecular approaches were much higher than by traditional method. These results suggested the higher health risk potential for hemodialysis patients from the up-to-standard water. The treatment process could also be optimized, based on the results of this study.

An Efficient and Mild Oxidation of α-Isophorone to Ketoisophorone Catalyzed by N-Hydroxyphthalimide and Copper Chloride

Lihua Chen,Ruiren Tang,Zhongying Li,Shan Liang 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.2

N-hydroxyphthalimide (NHPI) and copper chloride (CuCl2) were first utilized for aerobic oxidation of α-isophorone (α-IP) to ketoisophorone (KIP) and the effects of co-catalysts, temperature, reaction time, solvent,amount of CuCl2 and pressure of oxygen were investigated extensively. NHPI/CuCl2 turned out to be highly efficient to this oxidation with up to 91.3% conversion and 81.0% selectivity under mild conditions. And various hydrocarbons including benzylic compounds, cycloalkene and its derivatives were also oxidized smoothly under optimized conditions. Moreover, the possible reaction mechanism was proposed and verified by FT-IR spectra.

Fundamentals and Practical Design of Traction Inverter for Hybrid Electric Vehicles

Lihua Chen,Jin Wang 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

Electrification of vehicles is a non-reversible trend Current xEVs based applications have pushed the boundary of power electronics in terms of power density, efficiency and reliability New WBG based devices are evolving and promising for automotive applications Cost and reliability for WBGs are still the main barriers for automotive applications

Analysis of microbiomes in three traditional starters and volatile components of the Chinese rice wines

Chen Lihua,Ren Lixia,Dongna Li,Xia Ma 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.1

To understand the effect of microbial communityon the flavor of fermented rice wine, microbiomes inthree traditional starters (CMQ, NBQ, and YCQ) fromdifferent origins for making Chinese rice wines wereevaluated and the volatile components of their rice wineswere compared. The results showed that the dominantgenera in CMQ were Pantoea, Bacillus, Rhizopus, andCandida, the dominant microorganisms in NBQ were Pediococcus,Lactobacillus, Acetobacter, Weissella, Bacillus,Rhizopus, Candida, and Aspergillus, the dominantmicroorganisms in YCQ were Pediococcus, Lactobacillus,Leuconostoc, Weissella, Lactococcus, Ochrobactrum,Rhizopus, and Mucor. There were significant differences insensory properties of the wines brewed by three starters. Although the major aroma components were benzyl alcohol,2-octanone, benzoic acid, and phenethyl acetate, eachrice wine had its own main aroma components include1-octanol, 1-pentanol, ethyl acetate, etc. The resultsshowed that the different microbial communities in starterresults in the significant difference of the aroma componentsin its fermented rice wine.

An Efficient and Mild Oxidation of α-Isophorone to Ketoisophorone Catalyzed by N-Hydroxyphthalimide and Copper Chloride

Chen, Lihua,Tang, Ruiren,Li, Zhongying,Liang, Shan Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.2

N-hydroxyphthalimide (NHPI) and copper chloride ($CuCl_2$) were first utilized for aerobic oxidation of ${\alpha}$-isophorone (${\alpha}$-IP) to ketoisophorone (KIP) and the effects of co-catalysts, temperature, reaction time, solvent, amount of $CuCl_2$ and pressure of oxygen were investigated extensively. NHPI/$CuCl_2$ turned out to be highly efficient to this oxidation with up to 91.3% conversion and 81.0% selectivity under mild conditions. And various hydrocarbons including benzylic compounds, cycloalkene and its derivatives were also oxidized smoothly under optimized conditions. Moreover, the possible reaction mechanism was proposed and verified by FT-IR spectra.

中國傳統吉祥物論說

沈利華(Chen Lihua) 중국문화연구학회 2003 중국문화연구 Vol.0 No.2

Characterization and expression analysis of BcAMT1;4, an ammonium transporter gene in flowering Chinese cabbage

Lihua Zhong,Xinmin Huang,Yunna Zhu,Erfeng Kou,Houcheng Liu,Guangwen Sun,Riyuan Chen,Shiwei Song 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.4

Ammonium (NH 4+ ) is generated during many endogenous metabolic processes in the leaves of plants, and there is increasing evidence that ammonium transporters (AMTs) play important roles in NH 4 + transmembrane transport and distribution. However, the expression of diff erent AMT genes is tissue-type specifi c and their functions diff er. Information about AMT genes and their expression under diff erent environmental conditions in fl owering Chinese cabbage ( Brassica campestris L.) is currently limited. Here, we isolated and characterized an AMT gene, BcAMT1;4 , in fl owering Chinese cabbage. BcAMT1;4 was localized to the plasma membrane and complemented NH 4+ transport in NH 4+ uptake-defi cient yeast. The highest expression levels of BcAMT1;4 were detected in the fl owers and leaves of fl owering Chinese cabbage. The expression of BcAMT1;4 was induced by nitrogen defi ciency and signifi cantly inhibited by the reapplication of NH 4+ (NH 4 Cl or NH 4 NO 3 ). In contrast, when plants pre-cultured in nitrate were transferred to an NH 4+ nutrient solution, BcAMT1;4 expression was signifi cantly enhanced. BcAMT1;4 exhibited a diurnal expression pattern, with higher expression levels during the light period than during the dark period, and a peak expression at the later stage of the light period. Knowledge of AMT genes in fl owering Chinese cabbage will lay a foundation for enhancing our understanding of the functional roles of diff erent AMT members in the regulation of its growth by NH 4+ , as BcAMT1;4 seems to play an important role in leaf NH 4+ transport.

Environmental Regulation, Institutional Distance and China’s Outward Foreign Direct Investment in the Belt and Road Countries

Lihua Yang,Qi Chen,Huaqiang Su 한국무역연구원 2023 무역연구 Vol.19 No.6

Purpose – This study aims to explore the direct impact of environmental regulation on outward foreign direct investment (OFDI), and the moderating role of institutional distance in the relationship between the two, focusing on China’s OFDI in 58 countries along the Belt and Road (BRCs) during the period 2003-2021. Design/Methodology/Approach – The fixed effects model, DID model and panel threshold model are adopted in the basic estimation and heterogeneity tests. Findings – The results show that environmental regulation has a significant positive impact on China’s OFDI into the BRCs, and institutional distance has strengthened this positive impact. But this moderating effect can only be detected when the institutional distance is positive. Comparative analysis before and after the Belt and Road Initiative (BRI) showed that the impact of environmental regulation on China’s OFDI was greater after BRI than before BRI. Furthermore, the significant impact of environmental regulation on OFDI could only be observed in countries with relatively stringent environmental regulation. Threshold tests found that there is a threshold value in the impact of environmental regulation on OFDI. The DID estimation results showed that when the institutional level and environmental regulation level of the host country are lower than that of China, the BRI plays a greater role in promoting China’s OFDI into the BRCs. Research Implications – The findings have significant implications for understanding how China’s OFDI responds to the environmental regulation of the host countries, simultaneously considering the impact of institutional distance.

Cuticular hydrocarbon pattern as a chemotaxonomy marker to assess six species of thrips

Chen Ting,Li Qunchen,Qi Guojun,Gao Yan,Zhao Chenyin,Lu Lihua 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4

Thrips constitute several families of slender insects with fringed wings and unique asymmetrical mouthparts. They have become globally important pests, infesting a variety of agriculturally important crops. Species of thrips are difficult to identify due to their small size and similarities in morphology. Recently, in addition to morphology, both molecular and non-molecular taxonomic tools have been used to identify species differences. Insect cuticular hydrocarbons have been widely used in chemotaxonomy. In this study, a Thermal Separation Probe was used to identify the cuticular hydrocarbons of Frankliniella occidentalis, Frankliniella intonsa, Thrips palmi, Thrips hawaiiensis, Haplothrips chinensis and Gynaikothrips ficorum. We analyzed the hydrocarbon com position of adults in all 6 species, and in the larvae of F. occidentalis, T. hawaiiensis and T. palmi. The results showed that the composition of cuticular hydrocarbons differed between species. All 6 species of adults and 3 species of larvae were easily distinguishable by quantitative analysis of hydrocarbon profiles. These results provide a possible method for the identification of thrips.