EFFECT OF CARBONIZATION TEMPERATURE ON DIELECTRIC AND MICROWAVE ABSORBING PROPERTIES OF COBALT DOPED MESOPOROUS CARBON COMPOSITES

QIAN LIAN,HONGJING WU,LIUDING WANG,HAO WU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.3

Co doped mesoporous carbon composites (MC – Co) have been synthesized at di®erent carbon-ization temperatures via evaporation-induced multicomponent co-assembly strategy. Thenanostructures and chemical compositions of MC – Co composites were characterized by trans-mission electron microscope (TEM), scanning electron microscope (SEM) and X-ray di®ractionanalysis (XRD), etc. Their electromagnetic and microwave absorption properties were investi-gated in the frequency ranging from 2 GHz to 18 GHz. By the measurement of electromagneticparameters and theoretical simulation of re°ection loss (RL), the results showed that the mini-mum RL value of the optimum composite MC – Co-800 reached up to ? 41.9 dB at 13.5 GHz with athickness of only 1.80 mm, and the e®ective absorption bandwidth ( < ? 10 dB) is 4.4 GHz (from11.4 GHz to 15.8 GHz). This work demonstrated that the optimum carbonization temperature ofthe obtained composites is 800?C, which is critical to its electromagnetic properties. The excellentmicrowave absorption properties can be attributed to dielectric loss, Ohmic loss, and charac-teristic impedance matching. Therefore, the as-synthesized composites could be acted as a can-didate of microwave absorber, especially for the light weight demand.

P53 Arg72Pro and MDM2 SNP309 Polymorphisms Cooperate to Increase Lung Adenocarcinoma Risk in Chinese Female Non-smokers: A Case Control Study

Ren, Yang-Wu,Yin, Zhi-Hua,Wan, Yan,Guan, Peng,Wu, Wei,Li, Xue-Lian,Zhou, Bao-Sen Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Background: Cell cycle deregulation is a major component of carcinogenesis. The p53 tumor suppressor gene plays an important role in regulating cell cycle arrest, and mouse double minute 2 (MDM2) is a key regulator of p53 activity and degradation. Abnormal expression of p53 and MDM2 occurs in various cancers including lung cancer. Methods: We investigated the distribution of the p53 Arg72Pro (rs1042522) and MDM2 SNP309 (rs2279744) genotypes in patients and healthy control subjects to assess whether these single nucleotide polymorphisms (SNPs) are associated with an increased risk of lung adenocarcinomas in Chinese female non-smokers. Genotypes of 764 patients and 983 healthy controls were determined using the TaqMan SNP genotyping assay. Results: The p53 Pro/Pro genotype (adjusted OR = 1.55, 95% CI = 1.17-2.06) significantly correlated with an increased risk of lung adenocarcinoma, compared with the Arg/Arg genotype. An increased risk was also noted for MDM2 GG genotype (adjusted OR = 1.68, 95% CI = 1.27-2.21) compared with the TT genotype. Combined p53 Pro/Pro and MDM2 GG genotypes (adjusted OR = 2.66, 95% CI = 1.54-4.60) had a supermultiplicative interaction with respect to lung adenocarcinoma risk. We also found that cooking oil fumes, fuel smoke, and passive smoking may increase the risk of lung adenocarcinomas in Chinese female non-smokers who carry p53 or MDM2 mutant alleles. Conclusions: P53 Arg72Pro and MDM2 SNP309 polymorphisms, either alone or in combination, are associated with an increased lung adenocarcinoma risk in Chinese female non-smokers.

온라인 협업 도구 활용 한국어 수업 사례 : 상해 삼달대학교를 중심으로

배영련(Pei, Ying Lian),오선자(Wu, Shan Zi),최선진(Choi, Seon Jin),한나(Han, Na),오영화(Wu, Ying Hua) 글로벌교육연구학회 2021 글로벌교육연구 Vol.13 No.4

본 연구는 온라인 수업에서의 상호작용에 도움을 주며, 중국과 한국에서 동시에 사용할수 있는 다양한 온라인 협업 도구를 소개하고, 이 도구 네 가지(mentimeter, zoom, padlet, Quizizz)를 사용하여 중국인 대상 한국어 수업을 운영한 사례를 제시하였다. 그리고본 수업 운영에 대한 학습자 만족도를 살펴보기 위하여 2021학년도 3월 학기에 본 수업을수강한 상해 삼달대학교 한국어 학습자 75명을 대상으로 만족도 조사를 시행하였으며, 그결과 전체적인 수업 만족도는 평균 4.09였다. 또한, 온라인 수업에서의 상호작용 향상을위한 방법으로 활용한 온라인 협업 도구에 대한 전체적인 만족도는 4.24였으며, 각 도구의만족도는 mentimeter가 4.63, zoom이 4.15, padlet이 4.09, Quizizz 3.81 순이었다. 이 중에서 협업 도구에 대한 전체적인 만족도와 padlet 만족도가 수업 전체 만족도에 영향을미치는 것으로 분석되었다. 본 연구 결과에 따른 제언은 다음과 같다. 첫째, 온라인 협업도구 활용에 대한 학습자 만족도가 높기 때문에 한국어 수업에 활용할 수 있는 다양한 협업도구 연구를 바탕으로 수업 적용이 필요하며, 각 국 학습자용 교재 편찬이 필요하다고 할수 있다. 본 연구는 다양한 온라인 수업에서의 상호작용에 필요한 다양한 협업 도구를 소개하고 비대면 수업에서의 실제 활용방안을 제시했으며, 이에 대한 학습자들의 의견을 수렴했다는 점에서 의의가 있다. This study was to present a case of an Online Korean class using four online tools such as Mentimeter, Zoom, Padlet, Quizizz for helping the interaction of Korean and Chinese students. To investigate learner satisfaction, 75 Chinese students from Shanda University participated in a survey after the class. The results were as follows: First, the overall class satisfaction was 4.09, especially students were satisfied with using collaboration tools. Second, online tool satisfaction was 4.63 for Mentimeter, 4.15 for Zoom, 4.09 for Padlet, and 3.81 for Quizizz in the order of the program. Based on the results, it was suggested applications of various online tools into class and textbooks for learners. At last, it was significant that the case of online Korean class was presented with many online tools and learners satisfaction in this study.

Several factors affecting hypericin production of Hypericum perforatum during adventitious root culture in airlift bioreactors

Wu, S. Q.,Yu, X. K.,Lian, M. L.,Park, S. Y.,Piao, X. C. POLISH ACADEMY SCIENCES WARSAW 2014 Acta physiologiae plantarum Vol.36 No.4

Hypericum perforatum L. is a traditional medicinal plant for the treatment of depression and wound healing, and hypericin is one of the main effective active substances. To optimize the culture system for producing hypericin in adventitious root, this study used balloon-type airlift bioreactors to investigate the effect of air volume, inoculation density, indole-3-butyric acid (IBA) concentration and methyl jasmonate (MeJA) concentration on hypericin content and productivity during adventitious root culture. Hypericin content and productivity were improved with increasing air volume, and 0.1 vvm (air volume/culture volume/min) was optimal for hypericin production. Inoculation density also had a great effect on hypericin accumulation. Hypericin content and productivity were favorable in an inoculation density of 5.0 g l(-1) and decreased when inoculation densities were lower or higher than 5.0 g l(-1). Furthermore, 1.25 mg l(-1) IBA enhanced hypericin content and productivity, but too low (a parts per thousand currency sign0.50 mg l(-1)) or too high (a parts per thousand yen1.50 mg l(-1)) IBA concentrations decreased hypericin accumulation. MeJA concentration significantly affected biomass accumulation and hypericin production. The biomass decreased and hypericin production increased with increasing MeJA concentration. Optimum hypericin content (1.61 mg g(-1) DW) and productivity (15.57 mg l(-1)) were obtained at 350 mu M MeJA. The hypericin content in bioreactor-grown adventitious roots was lower than in 3-year field-grown plants, but significantly higher than that in in vitro-grown plantlets and 1-year field-grown plants. Thus, the bioreactor culture of adventitious roots can realize rapid and mass production of hypericin in H. perforatum.

Introduction of Telomerase Reverse Transcriptase Gene into Epidermal Stem Cells Derived from Human Skins

Lian-Qun Wang,De-Wu Liu,Wei Lan,Zun-Wen Lin,Pei-Xing Huang 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.4

Telomerase extends the proliferation and prevent replicative senescence in most somatic cells. Whether it has similar function in human epidermal stem cells remains to be determined. In this study, the human telomerase reverse transcriptase (hTERT) cytalytic subunit was introduced into epidermal stem cells derived from human fetal skins. The expression of hTERT mRNA, protein and telomerase activity in the transduced cells was observed by real time PCR (RT-PCR) techniques, western blot analysis and telomeric repeat amplification protocol enzyme-linked immunosorbent assay (TRAP-ELISA) assay, respectively. The proliferation of the transduced cells was examined. The results showed the introduction of hTERT into the epidermal stem cells upregulated the expression of the hTERT gene and protein. And also the hTERT-transduced epidermal stem cells exhibited significantly elevated telomerase activity and proliferation. These works establish the base of further gene modification, monoclone screening and cell differentiation mechanism. The ability to maintain the biological characteristics and proliferation of epidermal stem cells could have important applications in wound healing and skin tissue engineering.

STRUCTURAL DESIGN OF SPECIAL VEHICLE COMPONENTS BASED ON TOPOLOGY OPTIMIZATION

Wu Na,Liu Chao,Yang Zhian,Lian Zhaolun 한국자동차공학회 2023 International journal of automotive technology Vol.24 No.2

With the development of the economy, the demand for heavy-duty transportation vehicles is increasing. Due to the limitations of national standards and regulations, and the market’s requirements for the reliability and economy of the whole vehicle, it is urgent to optimize the structure of each part of the heavy-duty transportation vehicle. In this paper, the sub-frame of a certain type of cement mixer truck is taken as an example. Firstly, the finite element analysis method is used to find the structural defects of the existing products through static analysis and modal analysis of the existing products under full-load condition. Then, the density method is used to carry out topology optimization design of the existing sub-frame structure, and the optimized frame is checked. The conclusion is expected to provide a theoretical basis for the lightweight design of heavy transport vehicles.

The Clinicopathological and Prognostic Impact of 14-3-3 Protein Isoforms Expression in Human Cholangiocarcinoma by Immunohistochemistry

Wu, Qiao,Liu, Chang-Zheng,Tao, Lian-Yuan,Yu, Lan,Liu, Wei,Chen, Song-Sen,He, Xiao-Dong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

The 14-3-3 proteins are highly conserved, ubiquitous molecules involved in a variety of biologic phenomena, such as cell cycle control, and apoptosis. However, their expression in cholangiocarcinoma has not been previously characterized. In this paper, immunohistochemistry using specific anti-14-3-3 monoclonal antibodies was performed on formalin-fixed;, paraffin embedded archival tissue from 86 patients of cholangiocarcinoma. We also examined the correlation between expression and survival rate and clinicopathologic factors such as tumor location, tumor size, pathologic differentiation, lymphatic permeation, lymph node metastasis, and tumor stage. Positive 14-3-3 proteins expression was observed for 6 isoforms (${\beta}$, ${\sigma}$, ${\gamma}$, ${\theta}$, ${\delta}$, ${\eta}$) of these proteins in 86 patients of cholangiocarcinoma. ${\beta}$ and ${\sigma}$ isoform immunoreactivity was correlated with lymph node metastasis, tumor stage and patients' survival rate. In addition, ${\delta}$ isoform immunoreactivity showed trends with tumor location, tumor size, pathologic differentiation and tumor stage, while the ${\theta}$ isoform was correlated with pathologic differentiation. These results indicated that upregulated expression of some isoforms of 14-3-3 may be a common mechanism for evading apoptosis in cholangiocarcinoma, so that targeting 14-3-3 may be a novel promising strategy for the treatment of this tumor.

Vertical Migration of Sound Scatterers in the Southern Yellow Sea in Summer

Lian-Gang Lü,Jianjun Liu,Fei Yu,Wei Wu,Xiaodong 한국해양과학기술원 2007 Ocean science journal Vol.42 No.1

Acoustic volume backscattering strength data were collected and Conductivity Temperature Depth (CTD) measurements were conducted in the southern Yellow Sea in summer 2005 and 2006. The high temporal and vertical resolution acoustic data measured with a 307 kHz Acoustic Doppler Current Profiler (ADCP) and a 250 kHz acoustic Doppler profile (ADP) had dominant diel variation, which resulted from vertical migration of sound scatterers. Some scatterers congregating in the bottom layer in the daytime migrated upward at dusk, and migrated downward into the bottom layer at dawn. The migration speeds were estimated. More than 33 days data show that the diel migration varies with time. The feature of migration measured with ADCP and ADP is consistent to some extent with what is described in the study on vertical migration of zooplankton in the southern Yellow Sea with conventional net samples.

Acanthoic acid, a diterpene in Acanthopanax koreanum, protects acetaminophen-induced hepatic toxicity in mice

Wu, Y.-L.,Jiang, Y.-Z.,Jin, X.-J.,Lian, L.-H.,Piao, J.-Y.,Wan, Y.,Jin, H.-R.,Joon Lee, J.,Nan, J.-X. G. Fischer 2010 Phytomedicine Vol.17 No.6

The protective effect of a diterpenoid acanthoic acid (AA) isolated from Acanthopanax koreanum Nakai was investigated in acetaminophen (APAP)-induced hepatic toxicity. Drug-induced hepatotoxicity induced by an intraperitoneal (i.p.) injection of 300mg/kg (sub-lethal dose) of APAP. Pretreatment with AA (50 and 100mg/kg) orally 2h before the APAP administration attenuated the APAP-induced acute increase in serum aspartate aminotransferase (AST), and alanine aminotransferase (ALT) activites, replenished the depleted hepatic glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) activities, decreased malondialdehyde (MDA) level and considerably reduced the histopathological alterations in a manner similar to silymarin (Sily). Immunohistochemical analyses also demonstrated that AA could reduce the appearance of necrosis regions as well as caspase-3 and hypoxia inducible factor-1α (HIF-1α) expression in liver tissue. Our results indicated that AA protected liver tissue from the oxidative stress elicites by APAP-induced liver damage and suggestes that the hepatic protection mechanism of AA would relate to antioxidation and hypoxia factor on APAP-induced hepatotoxicity.

Bioreactor application on adventitious root culture of Astragalus membranaceus

Wu, S. Q.,Lian, M. L.,Gao, R.,Park, S. Y.,Piao, X. C. Springer Science + Business Media 2011 In vitro cellular & developmental biology. journal Vol.47 No.6