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( Pei Pei Li ),( Dong Dong Zhang ),( Xiao Juan Wang ),( Xiao Fen Wang ),( Zong Jun Cui1 ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1
A cellulose-degrading composite microbial system containing a mixture of microbes was previously shown to demonstrate a high straw-degrading capacity. To estimate its potential utilization as an inoculant to accelerate straw biodegradation after returning straw to the field, two cellulose-degrading composite microbial systems named ADS3 and WSD5 were inoculated into wheat straw-amended soil in the laboratory. The microbial survival of the inoculant was confirmed by a denaturing gradient gel electrophoresis (DGGE) analysis, whereas the enhancement of straw degradation in soil was assessed by measuring the mineralization of the soil organic matter and the soil cellulase activity. The results indicated that most of the DGGE bands from ADS3 were detected after inoculation into straw-amended autoclaved soil, yet only certain bands from ADS3 and WSD5 were detected after inoculation into straw-amended non-autoclaved soil during five weeks of incubation; some bands were detected during the first two weeks after inoculation, and then disappeared in later stages. Organic matter mineralization was significantly higher in the soil inoculants ADS3 and WSD5 than in the uninoculated controls during the first week, yet the enhanced degradation did not persist during the subsequent incubation. Similar to the increase in soil organic matter, the cellulase activity also increased during the first week in the ADS3 and WSD5 treatments, yet decreased during the remainder of the incubation period. Thus, it was concluded that, although the survival and performance of the two inoculants did not persist in the soil, a significant enhancement of degradation was present during the early stage of incubation.
Juan Wang,Haoming Liu,Haili Wang,Mingxun Cui,Qing Jin,Tie Jin,Fushun Cui,Taihua Cui,Cheng Yun Liang,김범식,Guanhao Li 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4
An protease from Actinidia arguta for improving meat tenderness was purified, characterizedfrom wild A. arguta fruit by ammonium sulfate precipitation, Sephdex G-25 gel filtration chromatography,and DEAE Sepharose Fast Flow ion exchange chromatography, and its activity was investigated. Thepurified protease was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis toobtain a single band of protease. The protease was purified successfully, and found to have a molecularweight of 23.8 kDa (mass spectrometry). The specific activity of the purified protease reached 53,428U/mg with a 25.5-fold purification factor and 9% activity recovery. Based on N-terminal sequencingresults, the A. arguta protease was derived from the class of actinidia proteases that have an Nterminalsequence of VLPDY VDWRS AGAVV. The protease was effective for tenderizing beef anddecomposing actomyosin, suggesting the potential application for improving meat tenderness.
Li Cui,Weiquan Bu,Jie Song,Liang Feng,Tingting Xu,Dan Liu,Wenbo Ding,Jianhua Wang,Changyang Li,Binge Ma,Yi Luo,Ziyu Jiang,Chengcheng Wang,Juan Chen,Jian Hou,Hong-mei Yan,Lei Yang,Xiao-bin Jia 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.3
Alantolactone is a sesquiterpene lactone isolatedfrom Inula helenium L. Although alantolactone possessesanti-inflammation and apoptosis-induction activities, theunderlying mechanism of anti-cancer effect on humanbreast cancer cells remains largely unknown. In this study,we explored the possibility of alantolactone as an apoptosis-inducing cytotoxic agent using MDA-MB-231 cells asin vitro model. Alantolactone significantly induced itsapoptosis, demonstrated by cell cycle analysis, annexinV-APC/7-AAD double staining and dUTP nick end labeling. Additionally, alantolactone triggered the mitochondrial-mediated caspase cascade apoptotic pathway, whichwas confirmed by increased Bax/Bcl-2 ratio, loss of MMP,release of cytc from mitochondria to cytoplasm, activationof caspase 9/3, and subsequent cleavage of PARP. Z-VADFMKpartially prevented apoptosis induced by alantolactone. Alantolactone provoked the production of ROS, whileNAC (a scavenger of ROS) reversed alantolactone-mediateddepolarization of MMP and apoptosis. Alantolactonemodulated the activities of MAPKs. As expected, cotreatmentwith SB203580, SP600125 or U0126 could reducedthe apoptotic rate. Furthermore, alantolactone decreasedthe protein expressions of p-NF-kB p65 and p-STAT3,increased p-c-Jun level in a dose-dependent manner. Thesefindings suggested that alantolactone possessed anticanceractivity via ROS-mediated mitochondrial dysfunctioninvolving MAPK pathway, and had an effect on the transcriptionfactors of NF-kB, AP-1 and STAT3.
Wang, Juan,Liu, Haoming,Wang, Haili,Cui, Mingxun,Jin, Qing,Jin, Tie,Cui, Fushun,Cui, Taihua,Liang, Chengyun,Kim, Bumsik,Li, Guanhao 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4
An protease from Actinidia arguta for improving meat tenderness was purified, characterized from wild A. arguta fruit by ammonium sulfate precipitation, Sephdex G-25 gel filtration chromatography, and DEAE Sepharose Fast Flow ion exchange chromatography, and its activity was investigated. The purified protease was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis to obtain a single band of protease. The protease was purified successfully, and found to have a molecular weight of 23.8 kDa (mass spectrometry). The specific activity of the purified protease reached 53,428 U/mg with a 25.5-fold purification factor and 9% activity recovery. Based on N-terminal sequencing results, the A. arguta protease was derived from the class of actinidia proteases that have an N-terminal sequence of VLPDY VDWRS AGAVV. The protease was effective for tenderizing beef and decomposing actomyosin, suggesting the potential application for improving meat tenderness.
Juan Liu,Jingquan Li,Li Feng,Hui Cao,Zhongli Cui 한국미생물학회 2010 The journal of microbiology Vol.48 No.6
Separation of bacterial cells from soil is a key step in the construction of metagenomic BAC libraries with large DNA inserts. Our results showed that when combined with sodium pyro-phosphate and homogenization for soil dispersion, sucrose density gradient centrifugation (SDGC) was more effective at separating bacteria from soil than was low speed centrifugation (LSC). More than 70% of the cells, along with some soil colloids,were recovered with one round of centrifugation. A solution of 0.8% NaCl was used to resuspend these cell and soil pellets for purification with nycodenz density gradient centrifugation (NDGC). After purification,more than 30% of the bacterial cells in the primary soil were extracted. This procedure effectively removed soil contamination and yielded sufficient cells for high molecular weight (HMW) DNA isolation. Ribosomal intergenic spacer analysis (RISA) showed that the microbial community structure of the extracted cells was similar to that of the primary soil, suggesting that this extraction procedure did not significantly change the the soil bacteria community structure. HMW DNA was isolated from bacterial cells extracted from red soil for metagenomic BAC library construction. This library contained DNA inserts of more than 200 Mb with an average size of 75 kb.
초산 지역어와 표준 한국어의 유사도 측정 -단어 간의 음운론적 유사도를 중심으로-
최소연(Cui, Xiao-juan),이금화(Li, Jin-hua) 국어국문학회 2019 국어국문학 Vol.0 No.189
본고는 “평안도 방언에 속하는 초산 지역어가 표준 한국어와 얼마정도 유사할까?” 라는 궁금증에서 시작하였다. 초산 지역어와 표준 한국어를 비교하는 데에는 음운, 어휘, 문법 등 영역이 포함될 수 있는데 본고에서는 평안북도 초산군 출신 화자가 사용하는 언어와 표준 한국어 단어가 어느 정도 유사한가를 측정하는 데에 중점을 두었다. 단어의 유사도는 화자의 발화 기간에 따라 달라질 것이라는 가설 하에, 이금화(2015)《초산(집안)지역어 텍스트》에서 선정한 60분의 발화 내용을 각각 10분, 20분, 30분으로 나누어 각 기간에 나타난 단어에 대해서 레벤시타인 거리 측정법을 적용하여 그 유사도를 수치화해 보았다. 측정 결과, 발화 기간에 따른 단어 간의 유사도는 각각 87%에서 85%로 변화가 있었고, 품사별로도 일정한 차이를 보였는데 감탄사의 유사도는 92%로 가장 높았고, 형용사의 유사도는 81%로 가장 낮았다. 그리고 모든 단어의 평균 레벤시타인 거리가 0.16으로 측정되었으므로 전체 유사도는 84%로 도출되었다. 본고에서는 단어 간의 유사도만 측정했으므로 초산 지역어 특유의 활용어미 등을 모두 고려했을 경우 그 유사도가 지금의 결과보다 현저히 낮아질 것으로 예상된다. This study began with the idea of how similar the Chosan dialect, which belonging to Pyongan-do dialect, is to standard Korean. Comparing the Chosan dialect with the standard Korean maybe can include all areas such as phonology, vocabulary, grammar and so on. The purpose of this study is to measure the phonological similarity between words of Chosan dialect and standard Korean. Under the assumption that the similarity will be depended on the time of speech, the 60 minute utterances selected by Chosan(Ji’an) Dialect Text were divided into 10 minutes, 20 minutes and 30 minutes, respectively, and the Levenshtein distance was applied as a method of measuring the similarity. As a result, the similarity according to the utterance period varies from 87% to 85%. And word classes also showed certain differences, that is the similarity of exclamations is highest at 92%, and that of adjectives is lowest at 81%. The average Levenshtein distance of these comparison words was 0.16, and it can be said that the similarity between Chosan dialect and standard Korean is 84%.