Carbon monoxide releasing molecule-2 suppresses stretch-activated atrial natriuretic peptide secretion by activating large-conductance calcium-activated potassium channels

Weijian Li,Sun Hwa Lee,Suhn Hee Kim 대한약리학회 2022 The Korean Journal of Physiology & Pharmacology Vol.26 No.2

Carbon monoxide (CO) is a known gaseous bioactive substance found across a wide array of body systems. The administration of low concentrations of CO has been found to exert an anti-inflammatory, anti-apoptotic, anti-hypertensive, and vaso-dilatory effect. To date, however, it has remained unknown whether CO influences atrial natriuretic peptide (ANP) secretion. This study explores the effect of CO on ANP secretion and its associated signaling pathway using isolated beating rat atria. Atrial perfusate was collected for 10 min for use as a control, after which high atrial stretch was induced by increasing the height of the outflow catheter. Carbon monoxide releasing molecule-2 (CORM-2; 10, 50, 100 μM) and hemin (HO-1 inducer; 0.1, 1, 50 μM), but not CORM-3 (10, 50, 100 μM), decreased high stretch-induced ANP secretion. However, zinc porphyrin (HO-1 inhibitor) did not affect ANP secretion. The order of potency for the suppression of ANP secretion was found to be hemin > CORM-2 >> CORM-3. The suppression of ANP secretion by CORM-2 was attenuated by pretreatment with 5-hydroxydecanoic acid, paxilline, and 1H-[1,2,4] oxadiazolo [4,3-a] quinoxalin-1-one, but not by diltiazem, wortmannin, LY-294002, or NG-nitro- L-arginine methyl ester. Hypoxic conditions attenuated the suppressive effect of CORM-2 on ANP secretion. In sum, these results suggest that CORM-2 suppresses ANP secretion via mitochondrial KATP channels and large conductance Ca2+-activated K+ channels.

NUMERICAL PROJECTION ON OCCUPANT THERMAL COMFORT VIA DYNAMIC RESPONSES TO HUMAN THERMOREGULATION

Weijian Li,Jiqing Chen,Fengchong Lan,Xijiao Zheng,Wenbo Zeng 한국자동차공학회 2022 International journal of automotive technology Vol.23 No.1

With the different physiological properties and thermal conditions, different body parts of passengers have inconsistent thermal sensations and thermal requirements in a highly non-uniform and transient vehicle cabin thermal environment. Determining the thermal comfort requirements for different body parts of a passenger is essential for effectively supplying warm air to the right human part especially for electric vehicles with energy-saving attributes. In this paper, a comprehensive numerical model that integrates human thermal regulation mechanism and dynamic environmental characteristics is established to calculate the thermal comfort for passengers via thermal responses to a dynamic environment. The numerical computation sets up such a model structure, firstly considering human thermal regulation functions into the thermal response to the in-cabin dynamic thermal distributions, then combining Berkeley thermal comfort model to identify the thermal comfort level at different body parts, that would implement total numerical simulations to get thermal comfort evaluation, independent of human subjective feedbacks. The model is validated by experiments with an acceptable error and implemented for a cabin heating case study. The models can effectively predict the thermal comfort and thermal requirements of various body parts in a dynamic environment with human thermoregulation, as an important tool for designing a non-uniform environment.

YTHDF1 promotes the proliferation, migration, and invasion of prostate cancer cells by regulating TRIM44

Li Weijian,Chen Gaohuang,Feng Zhenyu,Zhu Baoyi,Zhou Lilin,Zhang Yuying,Mai Junyan,Jiang Chonghe,Zeng Jianwen 한국유전학회 2021 Genes & Genomics Vol.43 No.12

Background Prostate cancer (PCa) is one of the most common malignancies in men. YTHDF1 may play an important role in promoting PCa progression, but there is no reports to date on YTHDF1 function in PCa. Objective This study explored whether YTHDF1 could regulate TRIM44 in PCa cells. Methods By querying the TCGA database, we evaluated YTHDF1 expression in PCa, the OS and DFS of YTHDF1, and the correlation between YTHDF1 and TRIM44 in PCa. We constructed vectors to interfere with YTHDF1 expression and overexpress TRIM44 to examine the role of YTHDF1 and TRIM44 in PCa cells. Diferentially expressed mRNAs were identifed by mRNA sequencing. The levels of YTHDF1, TRIM44, LGR4, SGTA, DDX20, and FZD8 were measured by qRT-PCR and WB was used to determine YTHDF1 and TRIM44 expression. A CCK-8 assay was used to assess cell proliferation. A Transwell chamber assay was used measure cell migration and invasion ability. Results YTHDF1 was highly expressed in both Pca tissues and cells. PCa patient prognosis with high YTHDF1 expression was relatively poor. Cell function experiments showed that inhibiting YTHDF1 expression decreased cell proliferation, migration, and invasion. RNA sequencing analysis revealed that YTHDF1 may promote PCa cell proliferation, migration, and invasion by modulating TRIM44 expression. Cell function experiments further verifed that YTHDF1 promoted PCa cell proliferation, migration, and invasion by regulating TRIM44. Conclusions YTHDF1 enhances PCa cell proliferation, migration, and invasion by regulating TRIM44.

Second-Order Power Analysis Attacks against Precomputation based Masking Countermeasure

Weijian Li,Haibo Yi 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.3

Precomputation look-up table based masking countermeasure is low-cost and secure against first-order DPA, therefore is more suitable for lightweight ciphers in resourceconstrained devices. In this paper, we investigate the resistance of this masking countermeasure against second-order power analysis attack under the attack context of the Hamming weight leakage and the precomputation masked S-box. We improve the Adapted CPA technique [1] to make a better use of this attack context. Our attack successfully reveals the secret key with and without electronic noise and algorithmic noise. The number of power traces required to reveal the secret key rises from 600(unprotected implementation) to 16,000.

An Ultra-Lightweight Side-Channel Resistant Crypto for Pervasive Devices

Weijian Li 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.11

Lightweight cryptography is equipped as security component, to secure those pervasive devices that are security and privacy sensitive. It has been conclusively proven that unprotected cryptographic implementations are vulnerable to side-channel attacks. In practice, area resource smaller than 3,000GE (5,000GE sometimes) may be available for security components in pervasive devices. This paper presents an ultra-lightweight first-order side-channel resistant crypto of KLEIN, which is a new family of lightweight block cipher that has advantages in both of software and hardware performances. The serial implementation of masked KLEIN requires 2102GE, while parallel implementation requires 4451GE, which are suitable for resource-constrained pervasive devices. Experimental results show that it is secure under First-order Power Analysis Attack, but still vulnerable to High-order Side-channel Attacks, with an exponential increase of the SCA data complexity.

Fast Three-Input Multipliers over Small Composite Fields for Multivariate Public Key Cryptography

Haibo Yi,Weijian Li 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.9

Since quantum computer attacks will be threats to the current public key cryptographic systems, there has been a growing interest in Multivariate Public Key Cryptography (MPKC), which has the potential to resist such attacks. Finite field multiplication is playing a crucial role in the implementations of multivariate cryptography and most of them use two-input multipliers. However, there exist multiple multiplications of three elements in multivariate cryptography. This motivates our work of designing three-input multipliers, which extend the improvements on multiplication of three elements in three directions. First, since multivariate cryptography can be implemented over small composite fields, our multipliers are designed over such fields. Second, since it requires multiplications of two and three elements, our multipliers can execute both of them. Third, our multipliers adapt table look-up and polynomial basis, since they are faster over specific fields, respectively. We demonstrate the improvement of our design mathematically. We implement our design on a Field-Programmable Gate Array (FPGA), which shows that our design is faster than other two-input multipliers when computing multiplication of three elements, e.g. multiplier with field size 256 is 28.4% faster. Our multipliers can accelerate multivariate cryptography and mathematical applications, e.g. TTS is 14% faster.

Member capacity-based progressive collapse analysis of transmission towers under wind load

Yong-Quan Li,Yong Chen,Guohui Shen,Wenjuan Lou,Weijian Zhao,Hao Wang 한국풍공학회 2021 Wind and Structures, An International Journal (WAS Vol.33 No.4

The wind-induced collapse of transmission towers has raised many concerns. Progressive collapse analysis is recognized as a promising method for the assessment of the collapse-resistant capacity of the transmission tower. The finite element model of an actual transmission tower is firstly built for the analysis, in which the dynamic behavior of the member in failure is taken into account to be in accord with the actual tower collapse. The analysis considering the main design load cases is conducted in advance to determine the case under which the tower has the potential to collapse. The incremental dynamic analysis in association with the explicit time integration algorithm is employed to perform a progressive collapse analysis, where the wind loads are simulated by using the linear filtering method, and the developed failure criterion with axial force and bending moment involved is based on the stability bearing capacity of the members. It is found the tower collapse begins with the horizontal bracing member near the waist. Then, the adjacent members, including the leg members, fail sequentially, and the tower collapses eventually with a shear-type failure. The demand to capacity ratio (DCR) in terms of bearing capacity of the member is defined to quantify the structural behavior, the location of the member that has the potential to fail, and when the initial failure occurs are thereby identified. It is concluded that compared to the member capacity-based analysis, the ultimate strain-based analysis, which is most likely to be an inelastic dynamic analysis permitting a large deformation, may overestimate the bearing capacity of the structure in wind-induced collapse.

Software Project Quality Evaluation Model for Web Application

WanJiang Han,WeiJian Li,HeYang Jiang,TianBo Lu,Xiaoyan Zhang 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.5

Quality is a major challenge throughout software projects, it is important to analyze and assess quality of a software project. Regular quality measurement is key when it comes to running a successful project. This paper presents a quantitative quality evaluation model of software project for Web application. This model uses Analytical Hierarchy Process (AHP), Fuzzy Mathematical theory and expert method to constrain quality metrics with respect. This model provides the weights of quality characteristics and sub-characteristics using analytic hierarchical process technique, which is on the basis of summering and analysis of historical test data for Web application. The experimental results indicate that this approach is effective for software project quality evaluation and can get good estimates.

Fast Hardware Implementations of Inversions in Small Finite Fields for Special Irreducible Polynomials on FPGAs

Haibo Yi,Weijian Li,Zhe Nie 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.9

Inversions in small finite fields are the most computationally intensive field arithmetic and have been playing a key role in areas of cryptography and engineering. The main algorithms for small finite field inversions are based on Fermat's little theorem, extended Euclidean algorithm, Itoh-Tsujii algorithm and other methods. In this brief, we present techniques to exploit special irreducible polynomials for fast inversions in small finite fields GF(2n) , where n is a positive integer and 0 < n < 16 . Then, we propose fast inversions based on Fermat's theorem for two special irreducible polynomials in small finite fields, i.e. trinomials and All-One-Polynomials (AOPs). Trinomials can be represented by polynomials xn + xm + 1 and AOPs can be represented by polynomials xn + Xn-1 + ... +1 , where m is a positive integer and 0 < m < n . Our designs have low hardware requirements, regular structures and are therefore suitable for hardware implementation. After that, our designs are programmed in Very-High-Speed Integrated Circuit Hardware Description Language (VHDL) by using integrated environment Altera Quartus II and implemented on a low-cost Field- Programmable Gate Array (FPGA). The experimental results on FPGAs show that our designs provide significant reductions in executing time of inversions in small finite fields, e.g. the executing time of inversion in GF(27) is 18.80 ns and the executing time of inversion in GF(212) is 29.57 ns.

Antimicrobial and Cytotoxic Activity of Endophytic Fungi from Lagopsis supina

Zhang Dekui,Sun Weijian,Xu Wenjie,Ji Changbo,Zhou Yang,Sun Jingyi,Tian Yutong,Li Yanling,Zhao Fengchun,Tian Yuan 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.4

In this study, five endophytic fungi belonging to the Aspergillus and Alternaria genera were isolated from Lagopsis supina. The antimicrobial activity of all fungal fermented extracts against Staphylococcus and Fusarium graminearum was tested using the cup-plate method. Among them, Aspergillus ochraceus XZC-1 showed the best activity and was subsequently selected for large-scale fermentation and bioactivity-directed separation of the secondary metabolites. Four compounds, including 2- methoxy-6-methyl-1,4-benzoquinone (1), 3,5-dihydroxytoluene (2), oleic acid (3), and penicillic acid (4) were discovered. Here, compounds 1 and 4 displayed anti-fungal activity against F. graminearum, F. oxysporum, F. moniliforme, F. stratum, Botrytis cinerea, Magnaporthe oryzae, and Verticillium dahliae with diverse MIC values (128–512 μg/ml), which were close to that of the positive control antifungal, actidione (64–128 μg/ml). Additionally, compounds 1 and 4 also exhibited moderate antibacterial activity against S. aureus, Listeria monocytogenes, Escherichia coli, and Salmonella enterica, with low MIC values (8–64 μg/ml). Moreover, compounds 1 and 4 displayed selective cytotoxicity against cancer cell lines as compared with the normal fibroblast cells. Therefore, this study proposes that the endophytic fungi from L. supina can potentially produce bioactive molecules to be used as lead compounds in drugs or agricultural antibiotics.