http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Lanlan Fang,Yiran Li,Sijia Wang,Yuxi Li,Hsun-Ming Chang,Yuyin Yi,Yang-yang Sun,Avinash Thakur,Peter C. K. Leung,Jung-Chien Cheng,Ying-Pu Sun 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-
Ovarian hyperstimulation syndrome (OHSS) is one of the most serious and iatrogenic complications that can occur during in vitro fertilization treatment. Although the pathogenesis of OHSS is not fully understood, vascular endothelial growth factor (VEGF) has been recognized as an important mediator of the development of OHSS. Transforming growth factor-beta-1 (TGF-β1) is known to regulate various ovarian functions. However, whether VEGF can be regulated by TGF-β1 in human granulosa cells has not been determined. In addition, the role of TGF-β1 in the pathogenesis of OHSS remains unknown. In the present study, we demonstrate that TGF-β1 stimulates VEGF expression in and secretion from both immortalized human granulosa-lutein (hGL) cells and primary hGL cells. Our results demonstrate that the SMAD2/3, ERK1/2, and p38 MAPK signaling pathways are involved in TGF-β1-induced VEGF expression and secretion. Using a mouse OHSS model, we show that the expression levels of TGF-β1 and VEGF are increased in the ovaries of OHSS mice. Blocking TGF-β1 signaling inhibits the development of OHSS by attenuating VEGF expression. Moreover, clinical results reveal that the protein levels of TGF-β1 and VEGF are increased in the follicular fluid of patients with OHSS, and that the levels of these two proteins in the follicular fluid are positively correlated. The results of this study help to elucidate the mechanisms by which VEGF expression is regulated in hGL cells, which could lead to the development of alternative therapeutic approaches for treating OHSS.
Analysis and Construction of a Quality Prediction System for Needle-Punched Non-woven Fabrics
Kuo Chung-Feng Jeffrey,Su Te-Li,Chiu Chin-Hsun,Tsai Cheng-Ping The Korean Fiber Society 2007 Fibers and polymers Vol.8 No.1
In this study, polyester and polypropylene staple fibers were selected as the raw material, and then processed through roller-carder, cross-lapper and needle-punching machine to produce needle-punched non-woven fabrics. First, the experiment was planned using the Taguchi method to select processing parameters that affect the quality of the needle-punched non-woven fabric to act as the control factors for this experiment. The quality characteristics were the longitudinal and transverse tensile strength of the non-woven fabric as well as longitudinal and transverse tear strength. The $L_{18}(2^1{\times}3^7)$ orthogonal array was selected for the experiment as it offered an improvement on the traditional method that wastes a lot of time, effort and cost. By using the analysis of variance(ANOVA) technique at the same time, the effect of significant factors on the production process of needle-punched non-woven fabrics could be determined. Finally, the processing parameters were set as the input parameters of a back-propagation neural network(BPNN). The BPNN consists of an input layer, a hidden layer and an output layer where the longitudinal/transverse tensile and tear strength of the non-woven fabric were set as the output parameters. This was used to construct a quality prediction system for needle-punched non-woven fabrics. The experimental results indicated that the prediction system implemented in this study provided accurate predictions.
Wu Carol P.,Chang Chia‐Jung,Li Cheng‐Hsun,Wu Yueh‐Lung 한국곤충학회 2021 Entomological Research Vol.51 No.4
The baculovirus expression system has been widely used for industrial-scale production of recombinant proteins. These recombinant proteins are expressed from recombinant baculoviruses carrying an expression cassette for a particular protein. Even though the baculovirus genome can accommodate large fragments of foreign genetic material, it has been shown that defective interfering particles (DIs) are generated over many passages of the recombinant virus. Defective interfering particles can affect the stability of the inserted gene, which can result in sub-optimal expression of the recombinant protein; this can, therefore, have a significant impact on production. The stability of exogenous genes in recombinant viruses over serial passage has not yet been investigated. As such, the present study set out to assess the effect of serial passage on the stability of the expression of an exogenous gene inserted into a recombinant baculovirus and its subsequent recombinant protein yield. To achieve this, a recombinant baculovirus carrying the classical swine fever virus E2 gene was serially passaged up to 20 times. The results showed that the E2 gene expression by the recombinant baculovirus was stable for up to 10 passages without any effect on recombinant E2 production in insect cells.
Lan Ting-Yuan,Lin Yen-Chun,Tseng Tai-Chung,Yang Hung-Chih,Kao Jui-Hung,Cheng Chiao-Feng,Lee Tai-Ju,Huang Shang-Chin,Lu Cheng-Hsun,Li Ko-Jen,Hsieh Song-Chou 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.2
Background/Aims: Rituximab is known to be associated with high hepatitis B virus (HBV) reactivation rate in patients with resolved HBV infection and hematologic malignancy. However, data regarding HBV reactivation (HBVr) in rheumatic patients receiving rituximab is limited. To assess the HBVr rate in hepatitis B surface antigen (HBsAg)-negative patients receiving rituximab for autoimmune diseases in a large real-world cohort. Methods: From March 2006 to December 2019, 900 patients with negative HBsAg receiving at least one cycle of rituximab for autoimmune diseases in a tertiary medical center in Taiwan were retrospectively reviewed. Clinical outcome and factors associated with HBVr were analyzed. Results: After a median follow-up period of 3.3 years, 21 patients developed HBVr, among whom 17 patients were positive for hepatitis B core antibody (anti-HBc) and four were negative. Thirteen patients had clinical hepatitis flare, while eight patients had HBsAg seroreversion without hepatitis. Old age, anti-HBc positivity, undetectable serum hepatitis B surface antibody level at rituximab initiation and a higher average rituximab dose were associated with a higher HBVr rate. There was no significant difference in the HBVr risk between rheumatoid arthritis and other autoimmune diseases. Among anti-HBc-negative patients, subjects without HBV vaccination at birth had an increased risk of HBVr (4/368, 1.1%) compared with those who received vaccination (0/126, 0%). Conclusions: In HBV endemic areas where occult HBV is prevalent, anti-HBc-negative patients, may still be at risk for HBVr after rituximab exposure. HBVr may still be considered in HBsAgnegative patients developing abnormal liver function after rituximab exposure, even in patients with negative anti-HBc.