RADIO JET FEEDBACK AND STAR FORMATION IN HEAVILY OBSCURED, HYPERLUMINOUS QUASARS AT REDSHIFTS ∼ 0.5-3. I. ALMA OBSERVATIONS

Lonsdale, Carol J.,Lacy, M.,Kimball, A. E.,Blain, A.,Whittle, M.,Wilkes, B.,Stern, D.,Condon, J.,Kim, M.,Assef, R. J.,Tsai, C.-W.,Efstathiou, A.,Jones, S.,Eisenhardt, P.,Bridge, C.,Wu, J.,Lonsdale, Co IOP Publishing 2015 The Astrophysical journal Vol.813 No.1

<P>We present Atacama Large Millimeter/submillimeter Array (ALMA) 870 mu m (345 GHz) data for 49 high-redshift (0.47 < z < 2.85), luminous (11.7 < log(L-bol/L-circle dot) < 14.2) radio-powerful active galactic nuclei (AGNs), obtained to constrain cool dust emission from starbursts concurrent with highly obscured radiative-mode black hole (BH) accretion in massive galaxies that possess a small radio jet. The sample was selected from the Wide-field Infrared Survey Explorer with extremely steep (red) mid-infrared colors and with compact radio emission from NVSS/FIRST. Twenty-six sources are detected at 870 mu m, and we find that the sample has large mid- to far-infrared luminosity ratios, consistent with a dominant and highly obscured quasar. The rest-frame 3 GHz radio powers are 24.7 < log P-3.0 GHz/W Hz(-1)) < 27.3, and all sources are radio-intermediate or radio-loud. BH mass estimates are 7.7 < log(M-BH/M-circle dot) < 10.2. The rest-frame 1-5 mu m spectral energy distributions are very similar to the 'Hot DOGs' (hot dust-obscured galaxies), and steeper (redder) than almost any other known extragalactic sources. ISM masses estimated for the ALMA-detected sources are 9.9 < log (M-ISM/M-circle dot) < 11.75 assuming a dust temperature of 30 K. The cool dust emission is consistent with star formation rates reaching several thousand M-circle dot yr(-1), depending on the assumed dust temperature, but we cannot rule out the alternative that the AGN powers all the emission in some cases. Our best constrained source has radiative transfer solutions with approximately equal contributions from an obscured AGN and a young (10-15 Myr) compact starburst.</P>

The influence of serial passage on the stability of an exogenous gene expression in recombinant baculovirus

Wu Carol P.,Chang Chia‐Jung,Li Cheng‐Hsun,Wu Yueh‐Lung 한국곤충학회 2021 Entomological Research Vol.51 No.4

The baculovirus expression system has been widely used for industrial-scale production of recombinant proteins. These recombinant proteins are expressed from recombinant baculoviruses carrying an expression cassette for a particular protein. Even though the baculovirus genome can accommodate large fragments of foreign genetic material, it has been shown that defective interfering particles (DIs) are generated over many passages of the recombinant virus. Defective interfering particles can affect the stability of the inserted gene, which can result in sub-optimal expression of the recombinant protein; this can, therefore, have a significant impact on production. The stability of exogenous genes in recombinant viruses over serial passage has not yet been investigated. As such, the present study set out to assess the effect of serial passage on the stability of the expression of an exogenous gene inserted into a recombinant baculovirus and its subsequent recombinant protein yield. To achieve this, a recombinant baculovirus carrying the classical swine fever virus E2 gene was serially passaged up to 20 times. The results showed that the E2 gene expression by the recombinant baculovirus was stable for up to 10 passages without any effect on recombinant E2 production in insect cells.

Lymphomatoid Granulomatosis: CT and FDG-PET Findings

Jonathan H. Chung,Carol C. Wu,Matthew D. Gilman,Edwin L. Palmer,Robert P. Hasserjian,Jo-Anne O. Shepard 대한영상의학회 2011 Korean Journal of Radiology Vol.12 No.6

Objective: Lymphomatoid granulomatosis (LG) is a rare, aggressive extranodal Epstein-Barr virus (EBV)-positive B-cell lymphoproliferative disease. The purpose of our study was to analyze the CT and fluorodeoxyglucose positron emission tomography (FDG-PET) findings of pulmonary LG. Materials and Methods: Between 2000 and 2009, four patients with pathologically proven pulmonary LG and chest CT were identified. Two of these patients also had FDG-PET. Imaging features of LG on CT and PET were reviewed. Results: Pulmonary nodules or masses with peribronchovascular, subpleural, and lower lung zonal preponderance were present in all patients. Central low attenuation (4 of 4 patients), ground-glass halo (3 of 4 patients), and peripheral enhancement (4 of 4 patients) were observed in these nodules and masses. An air-bronchogram and cavitation were seen in three of four patients. FDG-PET scans demonstrated avid FDG uptake in the pulmonary nodules and masses. Conclusion: Pulmonary LG presents with nodules and masses with a lymphatic distribution, as would be expected for a lymphoproliferative disease. However, central low attenuation, ground-glass halo and peripheral enhancement of the nodules/masses are likely related to the angioinvasive nature of this disease. Peripheral enhancement and ground-glass halo, in particular, are valuable characteristic not previously reported that can help radiologists suggest the diagnosis of pulmonary LG. Objective: Lymphomatoid granulomatosis (LG) is a rare, aggressive extranodal Epstein-Barr virus (EBV)-positive B-cell lymphoproliferative disease. The purpose of our study was to analyze the CT and fluorodeoxyglucose positron emission tomography (FDG-PET) findings of pulmonary LG. Materials and Methods: Between 2000 and 2009, four patients with pathologically proven pulmonary LG and chest CT were identified. Two of these patients also had FDG-PET. Imaging features of LG on CT and PET were reviewed. Results: Pulmonary nodules or masses with peribronchovascular, subpleural, and lower lung zonal preponderance were present in all patients. Central low attenuation (4 of 4 patients), ground-glass halo (3 of 4 patients), and peripheral enhancement (4 of 4 patients) were observed in these nodules and masses. An air-bronchogram and cavitation were seen in three of four patients. FDG-PET scans demonstrated avid FDG uptake in the pulmonary nodules and masses. Conclusion: Pulmonary LG presents with nodules and masses with a lymphatic distribution, as would be expected for a lymphoproliferative disease. However, central low attenuation, ground-glass halo and peripheral enhancement of the nodules/masses are likely related to the angioinvasive nature of this disease. Peripheral enhancement and ground-glass halo, in particular, are valuable characteristic not previously reported that can help radiologists suggest the diagnosis of pulmonary LG.

Analyses of the transcriptome of Bombyx mori cells infected with either BmNPV or AcMNPV

Yu-Wei Chen,Carol-P Wu,Tsai-Chin Wu,Yueh-Lung Wu 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

Baculoviruses are insect-specific DNA viruses with a restricted host range. Two examples of such includeAutographa californica nucleopolyhedrovirus (AcMNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV). AcMNPV is a commercially available and widely used baculovirus prototype which can infect 39 species in 13families. On the other hand, BmNPV is a major pathogen of silkworms which has developed high host specificityto Bombyx mori. Coincidentally, AcMNPV and BmNPV are highly homologous, but share no overlapping hostrange on a genomic level. In fact, these two similar viruses have extremely different infection outcomes inBombyx mori. We hypothesized that the determination of host specificity may depend on virus-host interactionsand several genes may be involved in determining host specificity. Therefore, we used next generation sequencing(NGS) to analyze transcriptome responses of hosts to these viruses. A transcriptome library was constructed,annotated, and grouped after sequence assembly. The comparison of gene expressions showed severalsignificant differences in the gene expression profiles of BmNPV and AcMNPV, especially in cases where genesinvolved with immune responses were verified by RNA interference. In addition, studies have shown that smallRNA plays a role in virus-host interaction, and further determines host specificity. Therefore, we screenedmicroRNA induced by AcMNPV infection, and then combined NGS data from cellular gens to predict possibleregulation networks. The manipulation of virus-host specificity can provide a breakthrough for the application ofbaculovirus in protein expression systems and the development of bio-control agents.