Special Issue of Professor Kiehwa Lee`s Retirement: Geophysical Environments of the Korean Peninsula : Articles ; Active Fault Study of the Yangsan Fault System and Ulsan Fault System, Southeastern Part of the Korean Peninsula

( Jai Bok Kyung ),( Kie Hwa Lee ) 대한지구물리학회 2006 지구물리 Vol.9 No.3

토끼 간조직의 Stimulatory Guanine Nucleotide 결합 단백의 αsubunit 에 대한 cDNA 염기서열에 관한 연구

이상훈,노연희,한중수,고재경 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

G proteins, a family of guanine nucleotide-binding protein superfamily, are involved in transmembrane signaling systems as transducers. In this study cDNAs encoding αsubunit of the stimulatory G protein (G α ) from rabbit liver were cloned and their nucleotide sequences were determined : a λgt 11 cDNA library from total cellular poly (A)' mRNA in rabbit liber was screened for recombinant λDNA that codes for G α with labeled probe. The probe used was an EcoRI digested 3' end fragment of cDNA for G α from olfactory neuroepithelium of rat. Six of the approximately recombinant clones were screened by in situ hybridization and by autoradiography, detecting sixteen positive clones. These clones were tested using polymerase chain reaction (PCR) technique and thirteen of them were turned out to have two sets of conserved sequence in αcDNA. Four clonse among them were selected form analysis of their cDNA sequences, showing the presence of two types of cDNA, namely and with the total length of nucleotide sequence of1.392bp and 1.613bp, respectively . From nucleotide sequence analysis the amino acid residues of -1 and α-2 were deduced : they contain 335 and 386 amino acid residues (including the initiator methionine), respectively. The calculated molecular weights for and were 38.9kd and 44.6kd, respectively. The significant difference in size between two cDNAs for G αseemed to be due to the assumption that alternative promoter and leading exon might be involved in transcribing the mRNA for . Two types of cDNA from rabbit liver shared over 95% of amino acid homology with that from rat olfactory neuroepichelium if deleted portions are not counted. More information on the cDNAs could be obtained through further studies such as Sl nuclease protection experiment, expression and mutation study.

대학생의 신체발육 및 건강상태에 관한 조사연구 -1980년도 충북대학교 신입생을 중심으로-

이경순, 노재섭, 박재영 忠北大學校 學生生活硏究所 1980 學生生活硏究 Vol.5 No.1

본문참고

PAGE를 사용한 Rotavirus 변이주의 검출

이재석,김경희,조양자 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.2

Viral RNA analysis by PAGEM a sensitive and highly specific test for detecting Rv in stool, was used to classify human Group A Rv int subgroups I an II and to find non-group A human Rv. Of 151 fecal samples positive for group A-specific monoclonal antibody (Mab)-based ELISA, 27(18%) belonged to subgroup I (short electropherotype) and 119 (79%) were identified as subgroup II (long electropherotype). Two samples contained mixed infections of the two electropherotypes. One isolate contained atypically migrating RNA with two extra segments between segment 4 and 5. Another isolate belong to neither one of the two electropherotypes, but they did not yield enough viral RNA to allow their classification. It is possible that reassortment viruses have emerged as a result of the patients' being infected simultaneously with belonging to the two electropherotypes. The unusual strains are being adapted to MA 104 cells and cultured for further antigenic analysis. PAGE yielded RNA typical of group A Rv in 5 Mab-based ELISA-negative stools, and thus raised the sensitivity of the ELISA.

한국전력공사 자료실의 운영 개선 방안

이재경 明知大學校 文獻情報學會 2004 文獻情報學論集 Vol.8 No.-

본 연구는 한국전력공사 자료실의 경영 및 기술정보의 입수, 분류, 입력, 저장, 검색 체계 개선 방안을 밝히고자 하였다.

폐 상피양 세포암에 특이한 Ribonuclease의 작용기전에 관한 연구

이성윤,지행옥,고재경 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

A neutral ribonuclease (RNase) specific to the epidermoid carcinoma of the lung was isolated from the lung cancer tissue to investigate processes involved in carcinogenesis and suppression of the lung cancer. Also studied were the substrate specificity and mechanism of action of the neutral RNase specific to the lung cancer. Neutral RNase activity in the lung tissue obtained by surgery was unchanged in four varieties of lung cancers (epidermoid carcinoma in 20 cases, 472±1859 umole/g/hr; adenocarcinoma in 5 cases, 5165±1575 umole/g/hr; karge cell carcinoma in 3 cases, 5870±2305 umole/g/hr; small cell carcinoma in 3 cases, 5405±2822 umole/g/hr; control in 31 cases, 4380±1520 umole/g/hr), indicating that RNase assay in the lung tissue could not be used as a biochemical marker for the lung cancer. Neutral RNases in the epidermoid carcinoma tissue of the lung were separated by a DEAE-cellulose column chromatography into 6 peaks, of which the peak Ⅰ neutral RNase isozyme was specific to the epidermoid cancer of the lung. High performance liquid chromatorgraphy (HPLC) and polyacrylamide gel electrophoresis (PAGE) patterns for peak Ⅰ protein from epidermoid carcinoma tissue of the lung appeared to be different from those of control lung tissue. The subpeak Ⅰ-5~8 (in HPLC pattern) that was supposed to be associated with RNase was greatly increased in the lung cancer, indicating that peak Ⅰ protein from epidermoid carcinoma tissue of the lung was specific to the lung cancer and that peak Ⅰ RNase specific to the cancer was located in HPLC subpeak Ⅰ-5~8. The peak Ⅰ neutral RNase was observed to be highly active toward poly C, poly AC and poly ACU and less active toward poly U and RNA, indicating that the peak Ⅰ neutral RNase was the secretory type of RNase. No activity was found toward polydezyribonucleotides and double stranded polyribonucleotides. Majority of products of poly C digest by the peak Ⅰ neutral RNase was analyzed to be oligoribonucleotides, indicating that the RNase was endonuclease in nature. Observations that the peak Ⅰ neutral RNase was specific to the epidermoid carcinoma of the lung and that the enzyme was endonuclease in nature suggested that the RNase might play an important role in process involved in the suppression of the lung cancer.

난소성 장액성 낭선암에 특이한 단백의 분리에 관한 연구

이상훈,장상근,고재경,김두상 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.1

In order to correlate changes in the nature of proteins in the serous cystadenocarcinoma tissue of the ovary with those in the cystic fluid and ascitic fluid of patients with the ovarian cancer, proteins in the ovarian tissue, cystic fluid and ascitic fluid of patients with serous cystadenocarcinoma were isolated by a DEAE-cellulose column chromatography and HPLC. (1)Concentrations of protein in the ovarian cancer tissue, cystic fluid and ascitic fluid of patients with serous cystadenocarcinoma were unchanged as compared with those of controls. (2)Proteins in the ovarian tissues were isolated by a DEAE-cellulose column chromatography into 7 peaks in both serous cystadenocarcinoma tissue and control ovarian tissue. The DEAE-peak Ⅲ protein was divided into two parts (peak ?? and ??) in the ovarian cancer, but not in the control, indicating that the DEAE-peak Ⅲ?? was specific to the ovarian cancer. The DEAE-peak Ⅴ common to both serous cystadenocarcinoma tissue and its control was further separated by a HPLC into 4 peaks each. Although the HPLC patterns for proteins of both tissues appeared to be different, presence of proteins specific to the ovarian cancer was not confirmed in the ovarian cancer tissue. (3)Proteins in the ovarian cystic fluid were isolated by a DEAE-cellulose column chromatography into 5 peaks for serous cystadenocarcinoma and 4 peaks for serous cyst as the control of which the peak Ⅳ protein was divided into two parts(peak ?? and ??) in the ovarian cancer, but not in the control. Two protein peaks (DEAE-peak ?? and ??) in the ovarian cancer, but not control. Two protein peaks (DEAE-peak ?? and Ⅷ) were observed to be specific to the ovarian cancer. (4)Proteins in the ascitic fluid were separated by a DEAE-cellulose column chromatography into 7 peaks for the ovarian cancer and 5 peaks for the serous cyst, of which the peak Ⅳ protein was also divided into two parts as in the cystic fluid of the ovarian cancer, but not in the control. Three protein peaks (peak Ⅲ, ?? and Ⅷ) appeared to be specific to the ovarian cancer. These results indicated that concentrations of proteins were unchanged, but the nature of proteins were changed in the cancer tissue, cystic fluid and ascitic fluid of patients with serous cystadenocarcinoma and that proteins specific to the ovarian cancer were present in at least one for the ovarian cancer tissue, two for the cystic fluid and three for the ascitic fluid. The proteins specific to serous cystadenocarcinoma in the cystic and ascitic fluid appeared to be released from the ovarian cancer tissue and could be used as biochemical markers for the cancer.

고정 스트레스 상태의 흰쥐에서 할로페리돌 전처치가 뇌생아민의 대사에 미치는 영향

이수복,양병환,김이영,이용성,고재경,김광일 漢陽大學校 精神健康硏究 1991 精神健康硏究 Vol.10 No.-

구강 악성 흑색종에서 PCNA 발현에 관한 면역조직화학적 연구

황경균,남윤우,이재일,이종호,심광섭,김명진 大韓顎顔面成形再建外科學會 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.6

Malignant melanoma arising from the mucosa of head and neck region was rare and showed poor prognosis. Some of malignant melanoma were transformed from benign melanotic lesion. Malignant melanoma had high cellular proliferation and rapid growth. The percentage of PCNA-positive cell (labeling index) is high in many malignant tumor. So we compared the pattern of PCNA expression in the melanotic lesion. We performed the immunohistochemical study in malignant melanoma(19 cases), benign melanotic macule(24 cases) and normal mucosa(20 cases) were diagnosed in Seoul National University Dental Hospital between 1980 and 2000. Positive PCNA staining was found mainly in malignant melanoma. The mean PCNA expression in malignant melanoma, melanotic macule, normal tissue were 29.2%, 1.4%, 0%, respectively. Significant differences in PCNA expression were noted between malignant melanoma and melanotic macule(p<0.01), normal mucosa(p<0.01). These result suggested that the PCNA expression seems to be used as a diagnostic indicator for malignancy in malignant melanoma and melanotic lesion.

시판 농후요구르트의 일반성분 및 탄수화물에 관한 연구

김은경,이수원,이재영 成均館大學校 科學技術硏究所 1990 論文集 Vol.41 No.2

This experiment was carried out to test for gross composition and physico-chemical properties of the yoghurt produced by 6 different domestic manufacturers between Mar. and Sep. 1989. 1. Total solid contents were between 13.81∼28.47%, protein 3.35∼4.70%, fat 1.80∼3.80%, ash 0.75∼0.96% and total sugar content of plain yoghurt was 4.42%, of strawberry yoghurt were 12.55∼16.99%. 2. The numbers of lactic acid bacteria ranged from 1.03 x 10 exp(9) to 1.54 x 10 exp(9) per ml. of the 3 products, and the other 3 products contained from 3.7 x 10 exp(8) to 8.2 x 10 exp(8) per ml. 3. Initial pH value of the products were between 4.02 and 4.30, titratable acidities were found to be 0.918% to 1.201%. 4. As sweetner, most of the products was used sucrose originated from strawberry preserve, but some did fructose and glucose.