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Yun Sun Lee,Hyun-Seung Park,Dong-Kyu Lee,Murukarthick Jayakodi,Nam-Hoon Kim,Sang-Choon Lee,Jinkyung Kim,Hana Lee,Dong-Yup Lee,Sung Won Kwon,Tae-Jin Yang 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Panax ginseng C.A. meyer (family: Araliaceae) is a perennial crop that has been widely used as a traditional medicine in Korea. Various P. ginseng cultivars exhibit a range of morphological and physiological traits as well as genetic diversity. To elucidate the differences of primary metabolism underlying such genetic diverstiy, we performed primary metabolite profiles in adventitious roots from five Panax ginseng cultivars using gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis revealed eight primary metabolites as biomarkers and allowed us to classify the five cultivars into three groups. We selected three cultivars to represent each group and analyzed their transcriptomes by Illumina sequencing. We inspected 100 unigenes involved in seven primary metabolite biosynthesis pathways and found that 21 unigenes encoding 15 enzymes were differentially expressed among the three cultivars. Integrated analysis of transcriptomes and metabolomes revealed that the ginseng cultivars differ in primary metabolites as well as in the putative genes involved in the complex process of primary metabolic pathways. Our data derived from this integrated analysis provide insights into the underlying complexity of genes and metabolites that co-regulate flux through these pathways in ginseng.
Chang, Dong-Jo,An, Hongchan,Kim, Kyoung-suk,Kim, Hyun Ho,Jung, Jinkyung,Lee, Jung Min,Kim, Nam-Jung,Han, Young Taek,Yun, Hwayoung,Lee, Sujin,Lee, Geumwoo,Lee, Seungbeom,Lee, Ju Sung,Cha, Jong-Ho,Park, American Chemical Society 2012 Journal of medicinal chemistry Vol.55 No.24
<P>Deguelin exhibits potent apoptotic and antiangiogenic activities in a variety of transformed cells and cancer cells. Deguelin also exhibits potent tumor suppressive effects in xenograft tumor models for many human cancers. Our initial studies confirmed that deguelin disrupts ATP binding to HSP90 and consequently induces destabilization of its client proteins such as HIF-1α. Interestingly, a fluorescence probe assay revealed that deguelin and its analogues do not compete with ATP binding to the N-terminus of HSP90, unlike most HSP90 inhibitors. To determine the key parts of deguelin that contribute to its potent HSP90 inhibition, as well as its antiproliferative and antiangiogenic activities, we have established a structure–activity relationship (SAR) of deguelin. In the course of these studies, we identified a series of novel and potent HSP90 inhibitors. In particular, analogues <B>54</B> and <B>69</B>, the B- and C-ring-truncated compounds, exhibited excellent antiproliferative activities with IC<SUB>50</SUB> of 140 and 490 nM in the H1299 cell line, respectively, and antiangiogenic activities in zebrafish embryos in a dose dependent manner (0.25–1.25 μM).</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jmcmar/2012/jmcmar.2012.55.issue-24/jm301488q/production/images/medium/jm-2012-01488q_0019.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jm301488q'>ACS Electronic Supporting Info</A></P>
Quercetin-Induced Glutathione Depletion Sensitizes Colorectal Cancer Cells to Oxaliplatin
Jinkyung Lee,Chan Ho Jang,Jisun Oh,Jong-Sang Kim 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Quercetin (QUE) is an antioxidant and bioactive phytochemical. Recently, it has been reported that QUE can inhibit glutathione reductase responsible for replenishing reduced form of glutathione (GSH) and thus lead to GSH depletion known to trigger cell death in certain contexts. In this study, we examined if QUE would decrease the GSH level in oxaliplatin (OXA)-treated HCT116 human colorectal cancer cells, thereby facilitating apoptotic cell death. Results showed that OXA alone decreased HCT116 cell viability and a combination with QUE further decreased it. Concurrently, a combination of QUE and OXA decreased intracellular GSH level while QUE alone increased it, compared to the control, suggesting that QUE may affect the GSH levels in HCT116 cells in the presence of OXA and augment the anticancer effect of OXA. Consistently, intraperitoneal administration of QUE significantly slowed the growth of HCT116 xenograft tumors in OXA-treated mice, compared to QUE or OXA alone treatment. Apoptosis-associated proteins were also highly expressed in the tumors treated in combination. These findings support the adjuvant potential of QUE on OXA-based chemotherapy in colorectal cancer cells.