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대기압 플라즈마에 의한 냉간압연 스테인리스 Micro-Cleaning판재의 에 관한 연구
오상훈,이성기,윤재홍,조동율,박봉규,이성규 국립7개대학공동논문집간행위원회 2004 공업기술연구 Vol.4 No.-
Atmospheric Plasma Cleaning (APC) was studied for the residual rolling oil contaminants on the surface of cold rolled Stainless Steel after optimizing APC conditions. Electrodes were placed at a distance of 10mm, with an RF power of 220W, and line velocity of 0.5m/min. The residue left by APC was 0.78mg/m², which was less than that of the approximate 1mg/m² by other classical cleaning methods. Especially, APC combined with pre-cleaning by 550℃Nitrogen gas gave a residue of only 0.01mg/m². Cleaning effect are also improved when oxygen gas is added to the atmospheric plasma. It was confirmed by the investigation of the official Korea Chemical Analysis Center that the APC-hot nitrogen method is superior to other methods. The residual contaminants for different methods were as follow : the classical method : about 1mg/m², APC : 0.78mg/m², and APC-hot nitrogen : 0.10mg/m².
Lee, Seung Woong,Song, Ja-Gyeong,Hwang, Byung Soon,Kim, Dae-Won,Lee, Yoon-Ju,Woo, E-Eum,Kim, Ji-Yul,Lee, In-Kyoung,Yun, Bong-Sik The Korean Society of Mycology 2014 Mycobiology Vol.42 No.2
We investigated a total of 335 samples of Korean native mushroom extracts as part of our lipoxygenase (LOX) inhibitor screening program. Among the mushroom-methanolic extracts we investigated, 35 exhibited an inhibitory activity greater than 30% against LOX at a concentration of 100 ${\mu}g/mL$. Especially, Collybia maculata, Tylopilus neofelleus, Strobilomyces confusus, Phellinus gilvus, P. linteus, P. baumii, and Inonotus mikadoi exhibited relatively potent LOX inhibitory activities of 73.3%, 51.6%, 52.4%, 66.7%, 59.5%, 100.0%, and 85.2%, respectively. Bioassay-guided fractionation led to the isolation of inoscavin A from the methanolic extract of P. baumii, which showed the most potent activity and was identified by spectroscopic methods. Specifically, inoscavin A exhibited potent LOX inhibitory activity with an $IC_{50}$ value of $6.8{\mu}M$.
Detection of Soybean Mosaic Virus Using RT-PCR
Yul-Ho Kim,Ok-Sun Kim,Bong-Choon Lee,Jae-Hwan Roh,Myoung-Ki Kim,Dae-Joon Im,Il-Bong Hur,Sang-Chul Lee 韓國作物學會 1999 Korean journal of crop science Vol.44 No.3
Reverse transcription and polymerase chain reaction (RT-PCR) assay was used to detect SMV strains. A pair of oligonucleotide primers were designed to include the cylindrical inclusion (CI) coding region between 4,176 to 5,560 nt. Amplification from the total RNA extracted from infected plants with SMV yielded a 1,385 bp DNA fragment. RT-PCR was shown to be 103 times more sensitive than the ELISA assay and it could detect a virus in 10-6 dilution. Restriction enzyme analysis of RT- PCR products using EcoR I showed that SMV isolates were classified into six groups according to the patterns of restriction fragments.
Consoramides A-C, New Zwitterionic Alkaloids from the Fungus Irpex consors
( Ji-yul Kim ),( Dae-won Ki ),( Yoon-ju Lee ),( Lee Su Ha ),( E-eum Woo ),( In-kyoung Lee ),( Bong-sik Yun ) 한국균학회 2021 Mycobiology Vol.49 No.4
In our ongoing search for new secondary metabolites from fungi, a basidiomycete fungus Irpex consors was selected for mycochemical investigation, and three new zwitterionic alkaloids (1-3) and five known compounds (4-8) were isolated from the culture broth (16 l) of I. consors. The culture filtrate was fractionated by a series of column chromatography including Diaion HP-20, silica gel, and Sephadex LH-20, Sep-Pak C<sub>18</sub> cartridge, medium pressure liquid chromatography (MPLC), and high pressure liquid chromatography (HPLC) to yield eight compounds (1-8). The structures of the isolated compounds were elucidated by the interpretation of nuclear magnetic resonance (NMR) spectra and high-resolution mass spectrometry (HR-MS). Their antioxidant and antibacterial activities were examined. The zwitterionic structures of three new sesquiterpene alkaloids (1-3) were determined together with five known compounds identified as stereumamide E (4), stereumamide G (5), stereumamide H (6), stereumamide D (7), and sterostrein H (8). This is the first report of the zwitterionic alkaloids in the culture broth of I. consors. Three new zwitterionic alkaloids were named as consoramides A-C (1-3).
Detection of Soybean Mosaic Virus Using RT-PCR
Kim, Yul-Ho,Kim, Ok-Sun,Lee, Bong-Choon,Roh, Jae-Hwan,Kim, Myoung-Ki,Im, Dae-Joon,Hur, Il-Bong,Lee, Sang-Chul The Korean Society of Crop Science 1999 Korean journal of crop science Vol.44 No.3
Reverse transcription and polymerase chain reaction (RT-PCR) assay was used to detect SMV strains. A pair of oligonucleotide primers were designed to include the cylindrical inclusion (CI) coding region between 4,176 to 5,560 nt. Amplification from the total RNA extracted from infected plants with SMV yielded a 1,385 bp DNA fragment. RT-PCR was shown to be $10^3$ times more sensitive than the ELISA assay and it could detect a virus in $10^{-6}$ dilution. Restriction enzyme analysis of RT- PCR products using EcoR I showed that SMV isolates were classified into six groups according to the patterns of restriction fragments.