Experimental study of reversal of multidrug resistance in human leukemia K562/DOX cells by toad venom

Hu, Pei,Qiu, Zhichao,Li, Yaohe,Liu, Anping,Chen, Zhixiong,Huliwen, Huliwen,Luo, Man,Guxuekui, Guxuekui,Xiaoyang, Xiaoyang,Xie, Ying,Lan, Hai Techno-Press 2021 Advances in nano research Vol.11 No.2

Acute leukemia is a malignant tumor originating from the hematopoietic system with the highest incidence and mortality. At present, the main clinical treatment of leukemia is still chemotherapy, during the course of which the multidrug resistance (MDR) will significantly reduce remission rate and disease-free survival rate of patients. MDR is the most important factor affecting refractory/recurrent acute leukemia. Therefore, reversing leukemia MDR is one of the best ways to improve the complete remission rate of refractory/recurrent acute leukemia, and the study of drugs and methods to overcome leukemia MDR has received extensive attention in the leukemia research field. This study was to primarily investigate the effects of Liushen pills on leukemia drug-resistant cell line K562/DOX in inhibiting growth, reversing resistance and inducing apoptosis in anticipation of providing useful cytological and molecular biological basis for the treatment of refractory/recurrent acute leukemia. The serum containing toad venom was prepared by means of Chinese drug serum pharmacology. MTT assay was used to detect the inhibitory rates of human leukemia cell line K562/DOX after being treated with the serum containing toad venom as well as daunorubicin, or with the serum containing toad venom alone at different time points. Real-time fluorescent quantitative analysis (RT-PCR) was performed to determine the effects of serum containing toad venom on the expression of BCL-2 mRNA in human leukemia cell line K562/DOX. Compared to the control group, toad venom showed inhibitory effects on K562/DOX cells; the expression level of BCL-2 mRNA in toad venom group were decreased, indicating that toad venom may reverse the resistance of K562/DOX cells by down-regulating the expression level of MDR1.

Case-Control Study of Diet in Patients with Cervical Cancer or Precancerosis in Wufeng, a High Incidence Region in China

Jia, Yao,Hu, Ting,Hang, Chuan-Ying,Yang, Ru,Li, Xiong,Chen, Zhi-Lan,Mei, Ye-Dong,Zhang, Qing-Hua,Huang, Ke-Cheng,Xiang, Qun-Ying,Pan, Xiu-Yu,Yan, Yu-Ting,Wang, Xiao-Li,Wang, Shao-Shuai,Hang, Zhou,Tang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Purpose: To investigate the diet of patients with cervical cancer and precancerosis in the Wufeng area, a high-incidence region in China. Methods: In the case group, 104 patients diagnosed with cervical cancer or cervical intraepithelial neoplasias (CINII/III) were recruited from the Wufeng area. Nine hundred thirty-six healthy women were selected from the same area as the matched controls. A questionnaire, which included questions about general lifestyle conditions, smoking and alcohol status, source of drinking water, green tea intake, and diet in the past year, was presented to all participants. Results: Green tea intake (P=0.022, OR=0.551, 95% CI=0.330-0.919) and vegetable intake (P=0.035, OR=0.896, 95% CI=0.809-0.993) were identified as protective factors against cervical cancer or CINII/III. There was no indication of any associations of other lifestyle factors (smoking status, alcohol status, source of drinking water) or diet (intake of fruit, meat/egg/milk, soybean food, onion/garlic, staple food and pickled food) with cervical cancer. Conclusions: The results suggest that eating more fresh vegetables and drinking more green tea may help to reduce the risk of cervical cancer or CINII/III in people of the Wufeng area.

Ginsenoside Rh2 attenuates microglial activation against toxoplasmic encephalitis via TLR4/NF-kB signaling pathway

Xiang Xu,Lan Jin,Tong Jiang,Ying Lu,Fumie Aosai,Hu-Nan Piao,Guang-Hua Xu,Cheng-Hua Jin,Xue-Jun Jin,Juan Ma,Lian-Xun Piao 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.5

Background: Ginsenoside Rh2 (GRh2) is a characterized component in red ginseng widely used in Korea and China. GRh2 exhibits a wide range of pharmacological activities, such as anti-inflammatory, antioxidant, and anticancer properties. However, its effects on Toxoplasma gondii (T. gondii) infection have not been clarified yet. Methods: The effect of GRh2 against T. gondii was assessed under in vitro and in vivo experiments. The BV2 cells were infected with tachyzoites of T. gondii RH strain, and the effects of GRh2 were evaluated by MTT assay, morphological observations, immunofluorescence staining, a trypan blue exclusion assay, reverse transcription PCR, and Western blot analyses. The in vivo experiment was conducted with BALB/c mice inoculated with lethal amounts of tachyzoites with or without GRh2 treatment. Results and conclusion: The GRh2 treatment significantly inhibited the proliferation of T. gondii under in vitro and in vivo studies. Furthermore, GRh2 blocked the activation of microglia and specifically decreased the release of inflammatory mediators in response to T. gondii infection through TLR4/NF-kB signaling pathway. In mice, GRh2 conferred modest protection from a lethal dose of T. gondii. After the treatment, the proliferation of tachyzoites in the peritoneal cavity of infected mice markedly decreased. Moreover, GRh2 also significantly decreased the T. gondii burden in mouse brain tissues. These findings indicate that GRh2 exhibits an antieT. gondii effect and inhibits the microglial activation through TLR4/NF-kB signaling pathway, providing the basic pharmacological basis for the development of new drugs to treat toxoplasmic encephalitis.

Continuous Sheet Metal Forming for Doubly Curved Surface Parts

Zhong-Yi Cai,Ying-Wu Lan,Ming-Zhe Li,Zhi-Qing Hu,Mi Wang 한국정밀공학회 2012 International Journal of Precision Engineering and Vol. No.

Continuous sheet metal forming (CSMF) is a novel technology for manufacturing doubly curved sheet metal parts. In this process,an upper flexible roll and two lower flexible rolls are employed as a forming tool, with the rotation of flexible rolls, the sheet metal is bent in the longitudinal and transverse directions simultaneously. In this study, the basic principle of CSMF system is introduced and the basic mathematical model to control the CSMF process is presented. Based on the analysis of the sheet metal deformation in longitudinal direction, the formulations to determine the finial curvature of the deformed sheet metal and to estimate the downward displacement of upper roll are obtained. Forming experiments for torus-shaped surface and saddle-shaped surface were carried out. The dimensional accuracy and smoothness of the CSMF parts were measured and analyzed. The experiment results confirm the validity of the presented mathematical models and shows that CSMF can be effectively used in forming of doubly curved surface parts.

A Systematic Review of Risk Factors for Brain Metastases and Value of Prophylactic Cranial Irradiation in Non-Small Cell Lung Cancer

Sun, Dian-Shui,Hu, Li-Kuan,Cai, Ying,Li, Xiao-Mei,Ye, Lan,Hou, Hua-Ying,Wang, Cui-Hong,Jiang, Yu-Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3

Background: The incidence of brain metastases (BM) varies in patients with non-small cell lung cancer (NSCLC), calls into question the value of prophylactic cranial irradiation (PCI). It is possible that clinicopathologic characteristics are associated with the development of BM, but these have yet to be identified in detail. Thus, we conducted the present meta-analysis on risk factors for BM and the value of PCI in patients with NSCLC. Methods: Eligible data were extracted and the risk factors for BM and the value of PCI in patients with NSCLC were analyzed by calculating the pooled odds ratio (OR). Heterogeneity was detected using Q and I-squared statistics, and publication bias was tested by funnel plots and Egger's test. Results: Six randomized controlled trials with a focus on the value of PCI and 13 eligible studies with a focus on risk factors for BM were included. PCI significantly reduced the incidence of BM in patients with NSCLC (p=0.000, pooled OR=0.34, 95% confidence interval = 0.37-0.59). Compared with non-squamous cell carcinoma, squamous cell carcinoma was associated with a low incidence of BM in patients with NSCLC (p=0.000, pooled OR=0.47, 95% confidence interval =0.34-0.65). The funnel plot and Egger's test suggested that there was no publication bias in the current meta-analysis. Conclusions: This meta-analysis provides statistical evidence that compared with non-squamous cell carcinoma, squamous cell carcinoma can be used as a predictor for BM in patients with NSCLC, and PCI might reduce the incidence of BM in patients with NSCLC, but does not provide a survival benefit.

Hydrogen sulfide inhibits the growth of Escherichia coli through oxidative damage

Liu-Hui Fu,Zeng-Zheng Wei,Kang-Di Hu,Lan-Ying Hu,Yan-Hong Li,Xiao-Yan Chen,Zhuo Han,Gai-Fang Yao,Hua Zhang 한국미생물학회 2018 The journal of microbiology Vol.56 No.4

Many studies have shown that hydrogen sulfide (H2S) is both detrimental and beneficial to animals and plants, whereas its effect on bacteria is not fully understood. Here, we report that H2S, released by sodium hydrosulfide (NaHS), significantly inhibits the growth of Escherichia coli in a dose-dependent manner. Further studies have shown that H2S treatment stimulates the production of reactive oxygen species (ROS) and decreases glutathione (GSH) levels in E. coli, resulting in lipid peroxidation and DNA damage. H2S also inhibits the antioxidative enzyme activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) and induces the response of the SoxRS and OxyR regulons in E. coli. Moreover, pretreatment with the antioxidant ascorbic acid (AsA) could effectively prevent H2S-induced toxicity in E. coli. Taken together, our results indicate that H2S exhibits an antibacterial effect on E. coli through oxidative damage and suggest a possible application for H2S in water and food processing.

The effects of cigarettes and alcohol on intestinal microbiota in healthy men

Lin Renbin,Zhang Yawen,Chen Luyi,Qi Yadong,He Jiamin,Hu Mengjia,Zhang Ying,Fan Lina,Yang Tao,Wang Lan,Si Misi,Chen Shujie 한국미생물학회 2020 The journal of microbiology Vol.58 No.11

Human intestinal microbiota is affected by the exogenous microenvironment. This study aimed to determine the effects of cigarettes and alcohol on the gut microbiota of healthy men. In total, 116 healthy male subjects were enrolled and divided into four groups: non-smoking and non-drinking (Group A), smoking only (Group B), drinking only (Group C), and smoking and drinking combined (Group D). Fecal samples were collected and sequenced using 16S rRNA to analyze the microbial composition. Short-chain fatty acid (SCFAs) levels in feces were determined by gas chromatography. We found that cigarette and alcohol consumptions can alter overall composition of gut microbiota in healthy men. The relative abundances of phylum Bacteroidetes and Firmicutes and more than 40 genera were changed with cigarette and alcohol consumptions. SCFAs decreased with smoking and alcohol consumption. Multivariate analysis indicated that when compared with group A, group B/C/D had higher Bacteroides, and lower Phascolarctobacterium, Ruminococcaceae_ UCG-002, Ruminococcaceae_UCG-003, and Ruminiclostridium_ 9 regardless of BMI and age. Additionally, the abundance of Bacteroides was positively correlated with the smoking pack-year (r = 0.207, p < 0.05), the abundance of predicted pathway of bacterial toxins (r = 0.3672, p < 0.001) and the level of carcinoembryonic antigen in host (r = 0.318, p < 0.01). Group D shared similar microbial construction with group B, but exerted differences far from group C with lower abundance of Haemophilus. These results demonstrated that cigarette and alcohol consumption separately affected the intestinal microbiota and function in healthy men; furthermore, the co-occurrence of cigarette and alcohol didn’t exacerbate the dysbiosis and cigarette played the predominated role on the alteration.

Genetic Analysis of the Liver Putative Tumor Suppressor (LPTS) Gene in Gastric Cancer

Hai Bing Zhang,Qu Zhang,Li Sa Wang,Fei Yan,Yun Lu,Zhi Qiang Wang,Jing Li,Liang Liang Zhang,Ying Yan Yu,Zheng Gang Zhu,Xiang Yin Kong,Lan Dian Hu 한국유전학회 2005 Genes & Genomics Vol.27 No.4