Pure 3D laparoscopy versus open right hemihepatectomy in a donor with type II and III portal vein variations

Kyungho Park,Ahmed Shehta,Jeong-Moo Lee,Suk Kyun Hong,Kyung Chul Yoon,Jae-Hyung Cho,Nam-Joon Yi,Kwang-Woong Lee,Kyung-Suk Suh 한국간담췌외과학회 2019 Annals of hepato-biliary-pancreatic surgery Vol.23 No.4

Backgrounds/Aims: Pure laparoscopic living donor right hemihepatectomy (PLDRH) has been performed in many experienced centers. However, portal vein variations still remain challenging thus disturbing the widespread of PLDRH in many centers. PLDRH when integrated with 3-dimensional laparoscopy and indocyanine green (ICG) near-infrared fluorescence cholangiography is safe and feasible. Methods: We reviewed 19 donors with separated right anterior and right posterior portal veins who underwent living donor right hemihepatectomy between January 2014 and December 2016. We compared the clinical outcomes of PLDRH and conventional open right hemihepatectomy (CDRH). Results: 6 donors (31.6%) underwent PLDRH while 13 donors (68.4%) underwent CDRH. There was no intraoperative complications, transfusions and open conversions in the PLDRH donors. The total operative time was longer in PLDRH (356.5 vs. 244.5 minutes, p=0.003). However, the length of hospital stay (8.5 vs. 9.0 days, p=0.703), blood loss (450.0 vs. 393.6 ml, p=0.557) and complication rate (16.6% vs.27.3%; p=0.327) did not differ between the two groups. Conclusions: PLDRH is safe and feasible in donors with type II and III portal vein variations. Further prospective comparative studies are needed to prove the safety and efficacy of PLDRH.

Perioperative risk factors of progressive chronic kidney disease following liver transplantation: analyses of a 10-year follow-up single-center cohort

Kyungho Lee,Junseok Jeon,Jong Man Kim,Gaabsoo Kim,김경아,Hye Ryoun Jang,Jung Eun Lee,Jae-Won Joh,Suk-Koo Lee,Wooseong Huh 대한외과학회 2020 Annals of Surgical Treatment and Research(ASRT) Vol.99 No.1

Purpose: The incidence of chronic kidney disease (CKD) has been increasing due to improved survival after liver transplantation (LT). Risk factors of kidney injury after LT, especially perioperative management factors, are potentially modifiable. We investigated the risk factors associated with progressive CKD for 10 years after LT. Methods: This retrospective cohort study included 292 adult patients who underwent LT at a tertiary referral hospital between 2000 and 2008. Renal function was assessed by the e stimated glomerular filtration rate (eGFR) using the Modification of Diet in Renal Disease formula. The area under the curve of serial eGFR (AUCeGFR) was calculated for each patient to assess the trajectory of eGFR over the 10 years. Low AUCeGFR was considered progressive CKD. Linear regression analyses were performed to examine the associations between the variables and AUCeGFR. Results: Multivariable analysis showed that older age (regression coefficient = -0.53, P < 0.001), diabetes mellitus (DM) (regression coefficient = -6.93, P = 0.007), preoperative proteinuria (regression coefficient = -16.11, P < 0.001), preoperative acute kidney injury (AKI) (regression coefficient = -14.35, P < 0.001), postoperative AKI (regression coefficient = -3.86, P = 0.007), and postoperative mean vasopressor score (regression coefficient = -0.45, P = 0.034) were independently associated with progressive CKD. Conclusion: More careful renoprotective management is required in elderly LT patients with DM or preexisting proteinuria. Postoperative AKI and vasopressor dose may be potentially modifiable risk factors for progressive CKD.

Clinical relevance of postoperative proteinuria for prediction of early renal outcomes after kidney transplantation

( Junseok Jun ),( Kyungho Park ),( Hyun Suk Lee ),( Kyo Won Lee ),( Jung Eun Lee ),( Jae Berm Park ),( Kyunga Kim ),( Wooseong Huh ),( Yoon-Goo Kim ),( Dae Joong Kim ),( Hye Ryoun Jang ) 대한신장학회 2022 Kidney Research and Clinical Practice Vol.41 No.6

Background: Proteinuria is associated with poor allograft and patient survival in kidney transplant recipients. However, the clinical relevance of spot urine protein-to-creatinine ratio (PCR) or albumin-to-creatinine ratio (ACR) as predictors of renal outcomes during the early postoperative period following kidney transplantation (KT) has not been determined. Methods: This single-center retrospective cohort study included 353 kidney transplant recipients who underwent KT between 2014 and 2017 and were followed up for more than 3 years. Among them, 186 and 167 recipients underwent living donor KT and deceased donor KT, respectively. The PCR and ACR were measured during the immediate postoperative period (within 7 days postoperatively), before discharge (2-3 weeks postoperatively), and 3-6 months postoperatively. Results: The median age of the patients was 51 years (interquartile range, 43-59 years), and 62.9% were male. An immediate postoperative PCR of ≥1 mg/mg was associated with old age, diabetes mellitus, high systolic blood pressure, delayed graft function, and donor factors (deceased donor KT, old age, and high serum creatinine concentrations). The PCR and ACR 3 to 6 months posttransplant were inversely associated with the estimated glomerular filtration rate at 1 year posttransplant. Deceased donor KT recipients with immediate postoperative PCR of ≥3 mg/mg showed a greater incidence of delayed graft function and lower estimated glomerular filtration rate before discharge than those with immediate postoperative PCR of <3 mg/mg. Conclusion: Early postoperative proteinuria is a useful biomarker to predict early renal outcomes after KT.

마이크로그루브 상 인간치은섬유아세포의 유전자 발현 분석: DNA microarray 연구

이경호,이성복,안수진,박수정,이석원,Lee, Kyungho,Leesungbok, Richard,Ahn, Su-Jin,Park, Su-Jung,Lee, Suk Won 대한치과보철학회 2017 대한치과보철학회지 Vol.55 No.4

목적: 마이크로그루브 상 인간치은섬유아세포의 유전자발현감식을 DNA microarray를 이용하여 연구하는 것이다. 재료 및 방법: Grade II 티타늄 시편을 이용하여 표면에 마이크로그루브(폭/깊이: $60{\mu}m/10{\mu}m$, E60/10)를 형성하고 불산으로 산에칭하여 실험군으로 사용하였다. 표면처리를 하지 않은 평활한 티타늄 표면(NE0)을 대조군으로 사용하였다. 실험군과 대조군에 인간치은섬유아세포를 배양한 후 total RNA를 추출하였다. Oligonucleotide microarray를 시행하여 실험군과 대조군 간 다양한 유전자 발현량의 변화를 확인하였다. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis를 통해 DNA chip의 발현 결과를 mapping하여 실험 조건에 따른 유전자 발현량의 변화를 pathway 수준에서 파악하였다. 결과: E60/10 마이크로그루브 표면과 NE0 표면에 대한 유전자 발현량 비교분석 결과, NE0 표면에 비하여 E60/10 마이크로그루브 표면에서 1.5배 이상 유의한 발현 차이를 보인 유전자는 123개, 2배 이상 유의한 발현 차이를 보인 유전자는 19개였다. 실험 조건에 따른 유전자 발현량의 변화를 KEGG pathway analysis를 통하여 확인하였고, 다양한 유전자 발현 결과들 중 대표적인 세포접착, 증식, 활성 관련 세포신호전달을 규명하였다. 결론: 마이크로그루브 표면은 다양한 유전자 발현 변화를 유도하고 관련 세포신호 전달을 유도한다. 본 연구의 결과에 따라서, 마이크로그루브는 유전자 발현 변화 및 세포신호 전달 활성화 등을 통한 세포활성도 증진을 필요로 하는 다양한 생체재료들의 표면으로 사용될 수 있다. Purpose: We aimed to investigate the gene expression of human gingival fibroblasts on microgroove surface using DNA microarray. Materials and methods: Microgrooves were applied on grade II titanium discs to have 0/$0{\mu}m$ (NE0, control group), 60/$10{\mu}m$ (E60/10, experimental group) of respective width/depth by photolithography. The entire surface of the microgrooved Ti substrata was further acid etched and used as the two experimental groups in this study. Human gingival fibroblasts were cultured in the experimental group and the control group, and total RNA was extracted. The oligonucleotide microarray was performed to confirm the changes of various gene expression levels between experimental group and control group. Changes of gene expression level were determined at the pathway level by mapping the expression results of DNA chips, using the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis. Results: Gene expression levels on E60/10 and NE0 were analyzed, there were 123 genes showing significant differences in expression more than 1.5 times on E60/10 microgrooved surface compared to NE0 surface, and 19 genes showing significant differences in expression more than 2 times. The KEGG pathway analysis confirmed the changes in gene expression levels under experimental conditions. Cell signaling, proliferation, and activity among the various gene expression results were identified. Conclusion: Microgrooved surfaces induce gene expression changes and related cell signaling. According to the results of this study, microgrooves can be used as the surface of various biomaterials which need to improve cell activity through gene expression changes and activation of cell signaling.

Keumsa Linteusan Suppresses Invasion of Cancer Cells through the Inhibition of Cellular Adhesion and MMP-9 Expression

Won-Jung Kim,Sung-Chang Hong,Eun-Ju Do,Kyungho Suk,윤익진,이원하 한국통합생물학회 2009 Animal cells and systems Vol.13 No.2

Extracts derived from various medical mushrooms have been reported to have antitumor and immunomodulatory properties. In order to investigate the antitumor activity of keumsa Linteusan, the water extract of Phellinus limteus, HT1080 cells, a human fibrosarcoma cell line, were treated with it and changes in cellular migration potential was tested in vitro. At a concentration range below 1,000 µg/ mL, Linteusan blocked, in a dose dependent manner, the migration of cells through Matrigel as well as Boyden chamber without affecting the viability of the cells. Prolonged treatment of HT1080 cells with Linteusan suppressed TNF- α induced production of matrix metalloproteinase (MMP)-9 as well as basal level expression of MMP-2. Linteusan also affected the adhesion of the cells to fibronectin-coated surfaces. The effect of Linteusan on cell signaling pathways was also tested. Linteusan specifically affected TNF-α induced phosphorylation of AKT in a dose-dependent manner, while phosphorylation levels of ERK remained unaffected. These data indicate that Linteusan blocks the migration of HT1080 cells by affecting various processes associated with cell migration such as the expression of matrix degrading enzymes, cell adhesion, and AKT-medicated cellular signaling pathways.

디젤을 이용한 차량용 히터의 연소특성

이상석(Sangseok Lee),이진석(Jinseok Lee),이도형(Dohyung Lee),석경호(Kyungho Suk) 한국유체기계학회 2006 유체기계 연구개발 발표회 논문집 Vol.- No.-

Most heater being used in vehicles has been used to a cabin heating by being supplied from a heat loss of the engine coolant for heat source efficiency. Recently, the heat loss is reduced by high efficiency in the engine. It becomes to require the combustion heater that directly burns the heater used in vehicles to provide heat source of the heater. The purpose of this study is to research a burner which will applicate combustion heater by the numerical analysis. There are the 5 different types of burners, which is designed by differently each design of the swirler. N-DODECANE by used the burner fuel is performed by numerical analysis in every 5 burner. The burner's efficiency testing is evaluated on the basis of the Exhaust gas temperature.

Cloning metallothionein gene in Zacco platypus and its potential as an exposure biomarker against cadmium.

Lee, Sangwoo,Kim, Cheolmin,Kim, Jungkon,Kim, Woo-Keun,Shin, Hyun Suk,Lim, Eun-Suk,Lee, Jin Wuk,Kim, Sunmi,Kim, Ki-Tae,Lee, Sung-Kyu,Choi, Cheol Young,Choi, Kyungho D. Reidel Pub. Co ; Springer 2015 Environmental monitoring and assessment Vol.187 No.7

<P>Zacco platypus, pale chub, is an indigenous freshwater fish of East Asia including Korea and has many useful characteristics as indicator species for water pollution. While utility of Z. platypus as an experimental species has been recognized, genetic-level information is very limited and warrants extensive research. Metallothionein (MT) is widely used and well-known biomarker for heavy metal exposure in many experimental species. In the present study, we cloned MT in Z. platypus and evaluated its utility as a biomarker for metal exposure. For this purpose, we sequenced complete complementary DNA (cDNA) of MT in Z. platypus and carried out phylogenetic analysis with its sequences. The transcription-level responses of MT gene following the exposure to CdCl2 were also assessed to validate the utility of this gene as an exposure biomarker. Analysis of cDNA sequence of MT gene demonstrated high conformity with those of other fish. MT messenger RNA (mRNA) expression and enzymatic MT content significantly increased following CdCl2 exposure in a concentration-dependent manner. The level of CdCl2 that resulted in significant MT changes in Z. platypus was within the range that was reported from other fish. The MT gene of Z. platypus sequenced in the present study can be used as a useful biomarker for heavy metal exposure in the aquatic environment of Korea and other countries where this freshwater fish species represents the ecosystem.</P>

시화호 유입 지천의 지표수와 퇴적물 용출수가 수서 지표생물에 미치는 급ㆍ만성 생태 독성 영향

박예나(Yena Park),김선미(Sunmi Kim),한선영(Sunyoung Han),이지연(Jiyoun Lee),박윤석(Yoon Suk Park),윤충식(Chung Sik Yoon),최경호(Kyungho Choi) 환경독성보건학회 2008 환경독성보건학회지 Vol.23 No.4

Acute and chronic toxicities of sediment elutriate and surface water samples collected at Lake Shihwa were evaluated using standard toxicity testing organisms including Vibrio fischeri, Daphnia magna and Moina macrocopa. Acute exposure resulted in toxic effects in all surface water or sediment elutriate samples, except for those collected from the reed swamp and Okgu stream. The rainy season influenced the toxicity of the water samples, presumably either by dilution of point discharge or through introduction of non-point source contaminants through runoff. In the sediment, elutriate and surface water samples, copper was detected above potentially lethal concentration, which may in part explain the observed toxicity, Considering acute toxicities of the surface water streams that direct to the Lake Shihwa, efforts should be warranted to control and reduce discharge of point and non-point sources along Lake Shihwa.

Dual Functionality of Myeloperoxidase in Rotenone-Exposed Brain-Resident Immune Cells

Chang, Chi Young,Song, Mi Jeon,Jeon, Sae-Bom,Yoon, Hee Jung,Lee, Dae Kee,Kim, In-Hoo,Suk, Kyungho,Choi, Dong-Kug,Park, Eun Jung Elsevier 2011 The American journal of pathology Vol.179 No.2

<P>Rotenone exposure has emerged as an environmental risk factor for inflammation-associated neurodegenerative diseases. However, the underlying mechanisms responsible for the harmful effects of rotenone in the brain remain poorly understood. Herein, we report that myeloperoxidase (MPO) may have a potential regulatory role in rotenone-exposed brain-resident immune cells. We show that microglia, unlike neurons, do not undergo death; instead, they exhibit distinctive activated properties under rotenone-exposed conditions. Once activated by rotenone, microglia show increased production of reactive oxygen species, particularly HOCl. Notably, MPO, an HOCl-producing enzyme that is undetectable under normal conditions, is significantly increased after exposure to rotenone. MPO-exposed glial cells also display characteristics of activated cells, producing proinflammatory cytokines and increasing their phagocytic activity. Interestingly, our studies with MPO inhibitors and MPO-knockout mice reveal that MPO deficiency potentiates, rather than inhibits, the rotenone-induced activated state of glia and promotes glial cell death. Furthermore, rotenone-triggered neuronal injury was more apparent in co-cultures with glial cells from <I>Mpo</I><SUP>−/−</SUP> mice than in those from wild-type mice. Collectively, our data provide evidence that MPO has dual functionality under rotenone-exposed conditions, playing a critical regulatory role in modulating pathological and protective events in the brain.</P>

구리와 벤조피렌에 노출된 피라미의 조직별 항산화반응

김우근(Woo-Keun Km),김정곤(Junkon Kim),김지원(Ji-Won Kim),이진욱(Jin-Wook Lee),임은석(Eun-Suk Lim),김선미(Sun-mi Kim),이상우(Sang-Woo Lee),최경호(Kyungho Choi),정진호(Jinho Jung),이성규(Sung-Kyu Lee) 한국환경독성학회 2011 한국독성학회 심포지움 및 학술발표회 Vol.2011 No.10