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Multiple stem cell traits of expanded rat bone marrow stromal cells
Yeon Lim, Jung,Jeun, Sin-Soo,Lee, Kyung-Jin,Oh, Ji Hyun,Kim, Seong Muk,Park, Sang In,Jeong, Chang Hyun,Kang, Seok-Gu Elsevier 2006 Experimental neurology Vol.199 No.2
<P><B>Abstract</B></P><P>Bone marrow stromal cells (BMSC) exhibit many traits of a stem cell population. Knowing that BMSC have the ability to self-renew, proliferate and differentiate into a variety of cell types, questions may arise as to whether these traits differ between the cells that have different expansion times. In this study, we examined the stem cell potentiality of BMSC through their characterization, proliferative capacity and the ability to differentiate into multiple lineages in the cultured 2nd passage cells and 10th passage cells. The results were as follows: (1) the 10th passage cells had a larger and more flatted morphology than the 2nd passage cells and also exhibited a decreased labeling for BMSC-related antigens such as CD90, CD73. (2) The cell proliferative capacity was approximately 2 times greater in the 2nd passage cells, and the apoptosis phenomenon was detected in the 10th passage cells. (3) The ability to differentiate into mesodermal tissue (osteocytes, adipocytes), as well as into ectodermal tissue (neurons) was more effective in the 2nd passage cells. Taken together, early stage BMSC would be a valuable cell source for various in vitro applications, as well as cell therapy.</P>
miRNA, PPI, 질병 정보를 이용한 마이크로어레이 데이터 통합 모델 설계
하경식(Kyung-sik Ha),임진묵(Jin-muk Lim),김홍기(Hong-gee Kim) 한국지능시스템학회 2012 한국지능시스템학회논문지 Vol.22 No.6
마이크로어레이는 수만 가지 이상의 DNA 또는 RNA를 기판위에 배열해 놓은 것이며 이 기술을 이용하여 대량의 유전자 발현을 탐색할 수 있게 되었다. 그렇지만 마이크로어레이는 실험자가 탐색하려는 특정 표현형에 대해서 설계된 실험방법을 이용하므로 제한된 숫자의 유전자 발현만을 관찰할 수 있다. 본 논문에서는 MicroRNAs(miRNAs)와 Protein-Protein Interaction(PPI) 정보를 포함하고 있는 데이터베이스를 활용하여 마이크로어레이 데이터의 의미적 확장 방법을 제시하고자 한다. 또한 Online Mendelian Inheritance in Man(OMIM) 및 International Statistical Classification of Diseases and Related Health Problems, 10th Revision(ICD-10)을 이용하여 질병 간 유전적 공통점 파악을 시도하였다. 이러한 접근방법을 통하여 새로운 생물학적 시각을 제공 할 수 있을 것으로 기대된다. A microarray is a collection of thousands of DNAs or RNAs arranged on a substrate, and it enables one to navigate large amounts of gene expression. However, a researcher uses his designed experimental methods to focus on particular phenotypes from the available mass of data. In this paper, we used MicroRNAs(miRNAs) and Protein-Protein Interation(PPI) databases to enhance and expand meanings in microarray data. Further, the expanded data are linked with the Online Mendelian Inheritance in Man(OMIM), and International Statistical Classification of Diseases and Related Health Problems, 10th Revision(ICD-10), in order to extract common genetic relationships between diseases. This approach, we expect, should provide new biological views.
위선암종에서 p53 단백 및 CREB-결합 단백의 면역조직화학적 발현양상
노태호(Tae Ho Noh),지경천(Kyung Choun Chi),임현묵(Hyun Muk Lim),이정효(Jung Hyo Lee),박용검(Yong Gum Park),김범규(Beom Gyu Kim),김미경(Mi Kyung Kim),김진수(Jin Soo Kim) 대한외과학회 2007 Annals of Surgical Treatment and Research(ASRT) Vol.72 No.6
Purpose: The wild-type p53 protein participates in suppressing cell transformations while its mutant forms has tumorigenic potential. Alterations in the structure of the p53 protein are one of the most common changes associated with human cancers. CREB-binding protein (CBP) and its homologue, p300, are transcriptional co-activators of various sequence-specific DNA-binding transcription factors and are involved in a wide range of cellular activities, such as DNA repair, cell growth, differentiation, and apoptosis. Several studies suggested that an association between p53 and p300 might account for the p53-responsible negative regulation. This study examined the relationship between p53 and CBP expression in terms of the clinicopathological factors and significance. Methods: The level of p53 protein and CBP expression was measured in 150 gastric adenocarcinoma patients, who had undergone a gastrectomy, and the relationship between p53 and CBP was examined. Immunohistochemical stain was performed on formalin-fixed paraffin-embedded sections using monoclonal anti-p53 and anti-CBP antibody. Results: 1. p53 protein was expressed in 46.3% (31/67) of early gastric cancers (EGC), 69.9% (58/83) of advanced gastric cancers (AGC)(P<0.05), 69.1% (65/94) of the intestinal type, 42.9% (24/56) of the diffuse type (P<0.05), 78.5% (55/70) of patients with a lymph node metastasis and 42.5% (34/80) of patients without a lymph node metastasis (P<0.01). 2. CBP expression was observed in 65% (61/94) of intestinal type, 51% (29/56) of the diffuse type (P>0.05), 47.8% (32/67) of EGC, 69.8% (58/83) of AGC (P<0.05), 68.6% (48/70) of patients with a lymph node metastasis and 52.5% (42/80) of patients without a lymph node metastasis (P>0.05). 3. p53 protein and CBP expression was coincidentally observed in 66.7% of gastric adenocarcinomas, and there was a significant correlation between the expression of both (P<0.05). Conclusion: That the expression of the p53 protein and CBP indirectly indicate the malignant potential of a cell, and may play an indirect role in the CBP and p53-mediated tumorigenic potential.
Bhang, Suk Ho,Cho, Seung-Woo,Lim, Jae Min,Kang, Jin Muk,Lee, Tae-Jin,Yang, Hee Seok,Song, Young Soo,Park, Moon Hyang,Kim, Hyo-Soo,Yoo, Kyung-Jong,Jang, Yangsoo,Langer, Robert,Anderson, Daniel G.,Kim, Wiley (John WileySons) 2009 Stem Cells Vol.27 No.8
<P>Ischemia is a potentially fatal medical event that is associated with as many as 30% of all deaths. Stem cell therapy offers significant therapeutic promise, but poor survival following transplantation to ischemic tissue limits its efficacy. Here we demonstrate that nanosphere-mediated growth factor delivery can enhance the survival of transplanted human adipose-derived stromal cells (hADSCs) and secretion of human angiogenic growth factors per cell, and substantially improve therapeutic efficacy of hADSCs. In vitro, in hypoxic (1% oxygen) and serum-deprived conditions that simulate in vivo ischemia, fibroblast growth factor-2 (FGF2) significantly reduced hADSC apoptosis and enhanced angiogenic growth factor secretion. In vivo, hADSCs delivered intramuscularly into ischemic hind limbs in combination with FGF2 resulted in significant improvements in limb survival and blood perfusion, as well as survival of the transplanted hADSCs and secretion of human angiogenic growth factors (i.e., vascular endothelial growth factor, hepatocyte growth factor, and FGF2). Interestingly, the majority of transplanted hADSCs were localized adjacent to the microvessels rather than being incorporated into them, suggesting that their major contribution to angiogenesis might be to increase paracrine secretion of angiogenic growth factors. This study demonstrates the potential of hADSCs in combination with growth factors for use in the treatment of ischemia.</P>
Effect of BDNF on the Neural Differatiation of Rat Bone Marrow Stromal Cells
( Ji Hyun Oh ),( Jung Yeon Lim ),( Seong Muk Kim ),( Sin Soo Jeun ),( Sang In Park ),( Chang Hyun Jeong ),( Seok Gu Kang ),( Kyung Jin Lee ) 한국조직공학과 재생의학회 2005 조직공학과 재생의학 Vol.2 No.4
Bone marrow stromal cells (BMSCs) exhibit multiple traits of a stem cell population, and they can expand many times in vitro and be induced to differentiate into multiple cell types. Several neurotrophins mediate the survival, differentiation, growth and apoptosis of neuron by binding to the neurotrophin factor receptor. Brain derived neurotrophic factor (BDNF) is known to have important functions for in neuronal survival in the nervous system, it enhances stem cells` ablilty to differentiate into neural cells and it promotes plasticity in vitro and in vivo. In this study, we investigated the effect of BDNF on the neural differentiation of rat BMSCs. The effect of BDNF on the neural differentiation of rat BMSCs was examined via imunocytofluorescence, western blotting and FACS analysis of specific neural markers in the neural induction condition and the BDNF-treated neural induction condition. According to our results, the expression of neural markers was higher in the BDNF treated neural induction condition compared to in the neural induction condition. Theses results show that BDNF promotes the neural differentiation of rat BMSCs.