검정금파리의 第二生殖週期 誘導

李鍾鎭,李熙權,李敬魯 全北大學校 1991 論文集 Vol.33 No.-

Induction of the second reproductive cycle and ovarian maturation, and changes of protein were investigated in the black blowfly, Phormia regina. A height in between hardboard and bottom of the experimental device based on the length of mouthpart and ovipositor was 2.5cm. Ovipositional rate of the first reproductive cycle in the experimental device was 81.2±6.1% without liver juice in the crop and midgut. During the second reproductive cycle terminal oocyte was 385, 977, 1121㎛ and ovary was 1732, 3819, 3946 ㎛ in length at 24, 48, and 60h after the second liver-feeding, respectively. Haemolymph protein increased 36h after the second liver-feeding and decreased thereafter while ovarian protein increased continuously until 60h in the second reproductive cycle. This result should be applicable as reference data for the embryo-physiological research of the second reproductive cycle in other Dipteran insects.

Porphyromonas endodontalis의 침투에 따른 혈관 내피세포의 유전자 발현

공희정,최경규,박상혁,이진용,최기운 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.6

During the last two decades, there has been an increasing interest in the impact of oral health on atherosclerosis and subsequent cardiovascular disease (CVD). To date, some periodontal pathogens including Porphyromonas gingivalis (P. gingivalis) have been reported to be relevant to CVD. Porphyromonas endodontalis (P. endodontalis), which shares approximately 87% sequence homology with P. gingivalis, is mostly found within infected root canals. However, recent studies reveal that this pathogen also resides in the dental plaque or periodontal pocket in patients with periodontitis. It has been shown that P. endodontalis invades human coronary artery endothelial cells (HCAEC) and coronary artery smooth muscle cells (CASMC). To evaluate whether P. endodontalis can participate in the progression of atherosclerosis and CVD, we examined the changes in transcriptional gene expression profiles of HCAEC responding to invaion by P. endodontalis in this study. The following results were obtained. 1. Porphyromonas endodontalis was invasive of HCAEC. 2. According to the microarray analysis, there were 625 genes upregulated more than two-folds, while there were 154 genes downregulated by half. 3. Upregulated genes were relevant to inflammatory cytokines, apoptosis, coagulation and immune response. Enhanced expression of MMP-1 was also noticeable. 4. The transcription profiles of the 10 selected genes examined by real-time PCR agreed well with those observed in the microarray analysis. Thus, these results show that P. endodontalis presents the potential to trigger and augment atherosclerosis leading to CVD. 본 연구에서는 Porphyromonas endodontalis와 죽상경화증 및 심혈관질환과의 관계를 알아보기 위해, P. endodontalis가 사람의 관상동맥 내피세포에 침투했을 때 나타나는 유전자 발현의 변화를 microarray와 real-time PCR로 측정하였고, 발현이 증가된 유전자 중에서 죽상경화증과 연관된 유전자들이 관련 KEGG pathway 상에서 유의성 있는 영향을 미치는지를 분석하여 다음과 같은 결과를 얻었다. 1. Porphyromonas endodontalis는 사람의 관상동맥 내피세포에 침투하였다. 2. Microarray 분석결과, 대조군보다 발현이 2배 이상 증가된 유전자는 625개였고, 1/2이하로 감소된 유전자는 154개였다. 3. 발현이 2배 이상 증가된 유전자 중에는 염증성 cytokine 및 chemokine, 세포자멸사, 혈액응고와 면역반응 관련 유전자들이 포함되었다. 4. Microarray 분석결과를 확인하기 위해 발현이 2배 이상 증가된 유전자 중에서 10개의 유전자를 선택하여 real-time PCR을 시행하였고, 그 결과 microarray에서와 마찬가지로 발현 정도가 대조군보다 모두 높게 나타났다. 따라서 P. endodontalis가 사람의 관상동맥 내피세포에 만성적으로 작용했을 때, 심혈관질환에서 중요한 부분을 차지하는 죽상경화증을 유발하는 위험 요소 중의 하나로 작용할 수 있을 것으로 판단된다. 이와 관련된 자세한 기전을 이해하기 위해서는 앞으로 더 많은 연구가 필요할 것으로 보인다.

바로끄 건축에 있어 動的 表現에 관한 연구

이공희 국민대학교 2003 造形論叢 Vol.22 No.-

본 연구의 목적은 바로끄 건축이 이룬 개념의 하나인 동적 표현의 관점을 통해서 그 건축에서 표현하거나 체험할 수 있는 동적의사들 속에서 그 표징을 밝히고, 그 표현의 개념과 원리를 체득함으로서 작금의 시대의사 속에 새로이 시사하는 바를 밝히고자 함에 있다. 따라서 이 논문은 두가지 접근으로 그 목적을 이룬다. 먼저 건축에 있어 동적표현을 개념하는 것이고, 다른 하나는 그 개념이 전개한 조형의 표징을 바로끄 건축 속에서 추적하는 것이다. 건축에 있어 동적 표현을 이루는 조형의사로 전개된 바로끄 건축에서 성과로 파동의 입면, 상호관입, 수축이완의 세가지 관점을 통해서 체득한다. The purpose of this research has tried to find the signs of dynamic intention which was expressed or experienced with a point of view of dynamic expression in Baroque architecture and has redefined dynamic intention of current thoughts in contemporary architecture by realizing the conceptions and principles of the expression. This thesis accomplishes its purpose with two points of view. One is conceptualizing the dynamic expression in architecture, the other is tracing the sign of formative arts which has developed with its concept in Baroque architecture. As a result, you may comprehend three points of view in architecture-wave of elevation, mutual infiltration, and oontraction/relaxation through Baroque architecture that has developed with the intention of formative arts on dynamic expression.

자발성 소뇌출혈에서 뇌전선화 단층촬영 소견이 초기의식 상태와 예후에 미치는 영향

이승희,정남,권창영,은종필 예수병원 1997 예수병원학술지 Vol.18 No.-

Alginate 배양내에서 Transglutaminase가 연골막세포에 미치는 영향

박정선,공차배,김충현,안현정,정미현,성상철,김희중,조현철,이상훈,이명철 대한정형외과연구학회 2003 대한정형외과연구학회지 Vol.6 No.2

토양환경지도 자료를 이용한 콩밭 토양의 화학성 비교

박지숙,이민진,정재원,김미혜,이서연,지윤미,한준호,김유학,공명석,한광현,노희명 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

It is important to interpret the soil chemical properties in order to effectively manage the farmland. This study was conducted to investigate the soil chemical properties of upland soybean field according to the soil series at 32 upland fields located in the parts of Gangwon-do. Soil sampling sites were selected by using the National Statistics DB and KSIS DB that is a soil environment information system provided by the Rural Development Administration. Soil samples were collected from 0~15 cm of top soil before seeding or transplanting, air-dried, passed through a 2 mm sieve, and analyzed for soil pH, EC, organic matter, available phosphorus (P2O5), exchangeable potassium, exchangeable calcium and exchangeable magnesium. The average chemical properties have exceeded the each nutrient optimum range level of RDA, except the organic matter in experimental soil 2015. And despite application of conventional fertilization, each soil series nutrient contents were change respectively. The results of this study suggest that conventional fertilization should be applied based on the analysis about the amount of accumulated nutrient contents in the soil.

Chiral differentiation of d- and l-isoleucine using permethylated β-cyclodextrin: infrared multiple photon dissociation spectroscopy, ion-mobility mass spectrometry, and DFT calculations

Lee, Sung-Sik,Lee, Jae-ung,Oh, Ju Hyeon,Park, Soojin,Hong, Yin,Min, Byeong Ki,Lee, Hyun Hee L.,Kim, Hugh I.,Kong, Xianglei,Lee, Sungyul,Oh, Han Bin The Royal Society of Chemistry 2018 Physical chemistry chemical physics Vol.20 No.48

<P>Chiral differentiation of protonated isoleucine (Ile) using permethylated β-cyclodextrin (perCD) in the gas-phase was studied using infrared multiple photon dissociation (IRMPD) spectroscopy, ion-mobility, and density functional theory (DFT) calculations. The gaseous protonated non-covalent complexes of perCD and d-Ile or l-Ile produced by electrospray ionization were interrogated by laser pulses in the wavenumber region of 2650 to 3800 cm<SUP>−1</SUP>. The IRMPD spectra showed remarkably different IR spectral features for the d-Ile or l-Ile and perCD non-covalent complexes. However, drift-tube ion-mobility experiments provided only a small difference in their collision cross-sections, and thus a limited separation of the d- and l-Ile complexes. DFT calculations revealed that the chiral distinction of the d- and l-complexes by IRMPD spectroscopy resulted from local interactions of the protonated Ile with perCD. Furthermore, the theoretical results showed that the IR absorption spectra of higher energy conformers (by ∼13.7 kcal mol<SUP>−1</SUP>) matched best with the experimentally observed IRMPD spectra. These conformers are speculated to be formed from kinetic-trapping of the solution-phase conformers. This study demonstrated that IRMPD spectroscopy provides an excellent platform for differentiating the subtle chiral difference of a small amino acid in a cyclodextrin-complexation environment; however, drift-tube ion-mobility did not have sufficient resolution to distinguish the chiral difference.</P>

Injury analysis of patients according to impact patterns involved in pedestrian traffic crashes

Lee, Hee Young,Youk, Hyun,II Lee, Jeong,Kang, Chan Young,Kong, Joon Seok,Sung, Sil,Kang, In Hye,Lee, Jung Hun,Kim, Oh Hyun,Jung, Woo Jin,Lee, Kang Hyun,Youn, Young Han,Park, Jong Chan Informa UK (TaylorFrancis) 2018 Traffic injury prevention Vol.19 No.suppl1

A crucial role of WW45 in developing epithelial tissues in the mouse

Lee, Joo-Hyeon,Kim, Tae-Shin,Yang, Tae-Hong,Koo, Bon-Kyoung,Oh, Sang-Phil,Lee, Kwang-Pyo,Oh, Hyun-Jung,Lee, Sang-Hee,Kong, Young-Yun,Kim, Jin-Man,Lim, Dae-Sik Wiley (John WileySons) 2008 The EMBO journal Vol.27 No.8

<P>The role and molecular mechanisms of a new Hippo signalling pathway are not fully understood in mammals. Here, we generated mice that lack WW45 and revealed a crucial role for WW45 in cell-cycle exit and epithelial terminal differentiation. Many organs in the mutant mouse embryos displayed hyperplasia accompanied by defects in terminal differentiation of epithelial progenitor cells owing to impaired proliferation arrest rather than intrinsic acceleration of proliferation during differentiation. Importantly, the MST1 signalling pathway is specifically activated in differentiating epithelial cells. Moreover, WW45 is required for MST1 activation and translocation to the nucleus for subsequent LATS1/2 activation upon differentiation signal. LATS1/2 phosphorylates YAP, which, in turn, translocates from the nucleus into the cytoplasm, resulting in cell-cycle exit and terminal differentiation of epithelial progenitor cells. Collectively, these data provide compelling evidence that WW45 is a key mediator of MST1 signalling in the coordinate coupling of proliferation arrest with terminal differentiation for proper epithelial tissue development in mammals.</P>

Collision-Free Multi-Robot Navigation Using Autonomous Object Selection

Lee, Kong Woo,Jeon, Jae D.,Kim, Doo Jin,Lee, Beom Hee Trans Tech Publications, Ltd. 2013 Applied mechanics and materials Vol.313 No.-

<P>For successful SLAM, landmarks for pose estimation should be continuously observed. Firstly, we proposed the object selection algorithm from 2D images and 3D depth maps without human’s supervision. We used the SIFT algorithm to obtain descriptors of point features inside the object, and the surface segmentation algorithm to obtain separated objects from point clouds of 3D depth maps. Automatically selected objects were tested by the threshold function using repeatability, distinctiveness and saliency whether the objects were suitable or not for reusing and sharing between the robots. Secondly, we suggested the closed-form solution to estimate the 3D pose of robots from the information of selected objects. Furthermore, we provided the effective way to accomplish the tasks using multi-robot by compensating the accumulated navigating errors and re-planning the collision-free motion of the robots using the extended collision map algorithm.</P>