Eleven Poems of Kim Kwang-gyun

Kim Kwang-gyun,Lee Sung-Il(번역자) The Academy of Korean Studies 1992 Korea Journal Vol.32 No.1

세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

방사선을 조사한 생쥐 장기에서의 Superoxide Dismutase 분포에 대한 연구

김양균,전현우,차종희,고광삼,이근배 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

The cytosolic and mitochondrial supreoxide dismutases (EC 1.15.1.1) were investigated in various organs, thyroid gland, lung, heart, liver, spleen and kidney, of normal and irradiated mice. The mouse organs were fractionated into cytosolic and mitochondrial fractions by differential centrifugation, and the supsroxidp dismutase was measured by the method of McCord and Fridovich. The mouse organs contain two types of superoxide dismutase ; one is localized in the cytosol and contains cupper and zinc, the other in the mitochondria and contains manganese. Superoxide dismutasc activity was found in all organs of mouse investigated, and maximal activity of enzyme was observed in liver. most of superoxide dismutase activity, 79-86%, was present in the cytosolic fraction, and the rest was associated with mitochondria. The activity of cytosolic superoxide dismutase was most prominent in the liver among those tissues assayed. However, that of mitochoadrial enzyme was highest in the heart. Thc specific activity of superoxide dismutase in mitochondria was found about 2-5 folds greater than that of the enzyme in cytosol and cyanide inhibit the activity of cytosolic enzyme but not mitochondrial enzyme, In irradiatcd mice, the enzyme activity and the specific activity of superoxide dismutase in cytosol and mitochondna of various organs were decreased with minimal value on 7th and l4th day after exposured by whole body-irradiation.

직렬 공진형 DC-DC CONVERTER 의 해석

金光一,丁元榮,盧采均 慶一大學校 1992 論文集 Vol.8 No.1

This paper described a study on the series resonant type DC-DC converter. A complete DC-DC converter analysis of the half-bridge H·F Voltage resonant converter is presented. Continuity and discontinuity of the operation mode divided into two classes in this circuit. Output voltage, current and power obtained to the circuit analysis.

습관성 흡연여성에서 후측 수부에 발생한 Buerger씨병

김삼,이남호,육청미,최영진,백대균,홍경순,두영철,한규록,오동진,유규형,임종윤,이광학,이영 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.7

뇌사에 대한 의사와 일반인의 태도 조사

손현균,김광일,김이영 大韓神經精神醫學會 1994 신경정신의학 Vol.33 No.1

In this attitude survey using Q-methodology, the authors attemped to identify the physician`s & public attitudes toward brain death. Thirty three statements related to attitude toward brain death were Q-sorted by 71 physicians & 71 laymen. The data were statistically analysed by Q-factor analysis. Five factors in physician group and six factors in layman group could be extracted. Characteristic features of five factors in the physicians were as follows: Factor A: Physicians of this factor positively approve of brain death by pragmatic reason. They agree to organ transplantation, but they resist donation of their own organs in organ transplantation. They are selfish and treasure their body. These people could be named as 「The egoistic brain death advocators」. Factor B: Physicians of this factor approve of brain death for organ transplantation. Most of them are religious. These people could be named as「The humanitarian brain death advocators」. Factor C: Attitude toward brain death in this group is vague. They agree to organ transplantation and also have intention of donation of their own organs and receipt of others. These people could be named as 「The transplantation devotees」. Factor D: These physicians are indifferent in attitude brain death and organ transplantation basically. These people could be named as「The bystanders」. Factor E: These physicians have no trust in phsysicians, religion and modern medicine. They agree to organ transplantation but strongly oppose to brain death. These people could be named as「The doctor mistrustees」. Characteristic features of factors in the laymen were as follows: Factor A: Subjects of this factor positively approve of brain death. They also agree to organ transplantation. They want to receive organs from others but do not intend to donate their own organs because they selfish and treasure their body. These people could be named as「The egoistic brain death advocators」. Factor B: People of this factor approve of brain death and organ transplantation. They are will to donate their own organs. They are mostly post graduated, single females. These people could be named as「The active brain transplantation advocators」. Factor C: People of this factor have somewhat negative attitude toward brain death but approve of organ transplantation. They have no intention of donation of their own organs in viewing traditional concept and attitude. They are mostly religious. These people could be named as 「The traditionals」. Factor D: These persons have no trust in physicians and religion. They have somewhat negative attitude toward brain death. They have no conspicuous opinion toward brain death and organ transplantation. These people could be named as「The doctor mistrustees」. Factor E: This factor was polarized. 「The extreme opposites to brain death」versue「The extreme brain death advocators」were named.「The extreme opposites to brain death」strongly oppsed to brain death and organ transplantation. Attitude of「The extreme opposites to brain death」are mirror image of「The extreme brain death advocators」. Discussion was carried out on the various attitude toward brain death in physicians and laymen.

정보 시스템을 이용한 항공기 착륙요율 결정 사례 연구 : 잔액 보상 방식에 의한 착륙요율 결정 방법 중심

유광의,김봉균 한국항공운항학회 1998 한국항공운항학회지 Vol.6 No.1

The purpose of this research is to look for the best description of calculating the reasonable Landing Fee. Landing Fee is consisted one of major revenues for maintaining an airport. Traditional Landing Fee Rate has been charged based on the weight factor; Maximum take-off weight, Maximum landing weight, or Maximum authorized weight. To achieve a better reliable value of Landing Fee Rate, The elements of Noise and Peak-Time have to be considered as well as the aircraft weight. This research designs the algorithms for calculating Landing Fee Rate and also Landing Fee, based on the aircraft weight. The Network is also applied to above. That is, CGI(Common Gate Interface) is constructed to interface the terminal of calculating Landing Fee Rate, and the terminal of collecting and transmitting the data such as the Weight. The computer language on the CGl was made by C++ and PERL. The main point of this research is to integrate the airport and Information System and to construct the database which is based on the different perspective of calculating Landing Fee Rate. However, the result of the most efficient and reliable will be computed based on above. This research will broaden the range of application up to the each case of airports.

영남 방언과 호남 방언의 운율 비교 : 대화체 월의 운율을 중심으로

김차균,고광모,김주원,정원수 충남대학교 문리과대학 어문연구회 2000 語文硏究 Vol.34 No.-

In this article, we compared the prosody of Yeongnam and Honam dialect, which is tone dialect and semi-tone dialect respectively. The data is that of Changweon dialect of Gyeongnam Province and Damyang dialect of Jeonnam Province chiefly. In some case the data of Yecheon dialect of Gyeongbuk Province was also dealt. For analysis of colloquial sentences, we employed three-way analysis: First, 1062 Changweon and Damyang colloquial sentences were dealt in auditory method. Secondly, 20 sentences of two dialects and 30 words of Changweon dialect were dealt with experimental method. Thirdly, for comparison of toponymy, the tone of the station names was investigated. In this article, we analyzed sentential tone of two dialects. For that, distribution of tone pattern and reduction or weakening of tone pattern in a sentence was investigated. The descripton about tone reduction was firstly done in this study.

구강 악성 흑색종에서 PCNA 발현에 관한 면역조직화학적 연구

황경균,남윤우,이재일,이종호,심광섭,김명진 大韓顎顔面成形再建外科學會 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.6

Malignant melanoma arising from the mucosa of head and neck region was rare and showed poor prognosis. Some of malignant melanoma were transformed from benign melanotic lesion. Malignant melanoma had high cellular proliferation and rapid growth. The percentage of PCNA-positive cell (labeling index) is high in many malignant tumor. So we compared the pattern of PCNA expression in the melanotic lesion. We performed the immunohistochemical study in malignant melanoma(19 cases), benign melanotic macule(24 cases) and normal mucosa(20 cases) were diagnosed in Seoul National University Dental Hospital between 1980 and 2000. Positive PCNA staining was found mainly in malignant melanoma. The mean PCNA expression in malignant melanoma, melanotic macule, normal tissue were 29.2%, 1.4%, 0%, respectively. Significant differences in PCNA expression were noted between malignant melanoma and melanotic macule(p<0.01), normal mucosa(p<0.01). These result suggested that the PCNA expression seems to be used as a diagnostic indicator for malignancy in malignant melanoma and melanotic lesion.

통조림용 가다랑어육의 식품성분

오광수,김정균,김인수,이응호,김복규 國立統營水産專門大學 附設 水産科學硏究所 1990 수산과학연구소보고 Vol.2 No.-

가다랑어 혈합육을 보다 효율적으로 이용하기 위한 일련의 기초연구로서, 가다랑어 혈합육의 지질성분, 함질소엑스분 및 구성아미노산 등을 보통 육과 함께 분석하여 비교, 검토하였다. 보통육은 혈합육에 비해 수분, 조단백질 함량은 약간 많은 반면, 조지방과 회분 함량은 혈합육이 약간 많았다. 휘발성염기질소량은 보토육이 22.7mg/100g, 혈합육이 46.9mg/100ㅎ으로 혈합육이 보통육에 비해 선도저하가 훨씬 빨랐다. 보통육과 혈합육의 총지질 조성은 각각 중성지질 79.7%, 71.9%, 당지질, 6.8%, 9.5%, 인지질13.5%, 18.6%로 이루어져 있었다. 중성지질의 성분은 free fatty acid ac triglyceride, 인지질은 phosphatidyl ethanolamine, phosphatidyl, 인지질은 phosphatidyl choline이 주성분이었고, lysogud 인지질의축적도 확인되었다. 총지지르 중성, 당 및 인지질의 축적도 확인되었다. 종지질, 중성, 당 및 인지질의 지반산 조성에서 보통육은 16:0 및 18:1을 주체로 한 포화산 및 모노엔산의 조성비가 혈합육보다 높았으며, 혈합육은 폴리엔산, 특히 22:6의 비율이 월등히 높았다. 16:0. 18:0, 18:1, 20:5 및 22:6 등이 주요 구성지방산이었다. 유리아미노산의 조성은 총함량에서 보통육 쪽이 많았고, 보통육에는 histidine, 혈합육에는 tauring의 보통육에서는 680.9mg.100g 정도 전존해 있었으나, 혈합육에서는 IMP의 분해가 급속히 진행되어 73.1mg/100g에 지나지 않았다. TMAO 및 TMA는 보통육에 비해 혈합육에 휠씬 많이 함유되어 있었고, total creatinine은 보통육 쪽이 많았다. 구성아미노산의 조성은 양시료 간에 별차이가 없었고, glutamic acid, aspartic acid, lysine, leucine, alanine, isoleucine 및 arginine 등이 주요 구성아니노산이었다. The lipid components, nitrogenous extracts and amino acids of dark muscle(DM) of skipjack (Katsuwonus pelamis) were analyzed and compared with those of white muscle(WM). WM was higher in moisture and crude protein content, and lower in crude lipid and ash content than those of DM. Contents of volatile basic nitrogen in WM and DM were 22.7mg/100g and 46.9mg/100g. Total lipid(TL) of WM and DM consisted of 79.7%, 71.9% neutral lipid(NL), 6.8%, 9.5% glycolipid(GL), and 13.5%, 18.6% phospholipid(PL), respectively. NL was mainly composed of free fatty acid, triglyceride, and PL was mainly occupied by phosphatidyl ethanolamine, phosphatidyl choline. Also lysophosphatidyl choline and lysophosphatidyl ethanolamine were identified in PL. In fatty acid composition of TL, NL, GL and PL, WM revealed higher contents in saturates and monoenes such as 16 : 0, 18 : 1, while DM showed higher contents in polyenes such as 22 : 6 especially. The major fatty acids of these samples were generally 16 : 0, 18 : 0, 18 : 1, 20 : 5 and 22 : 6. Contents of total free amino acids from WM and DM were 5,982.3mg/100g and 4,450.7mg/100g (dry base). Of free amino acids, Tau concentration was much higher in DM than in WM, Ala, Gly, Met, Arg, Thr were also high in DM. But His was much higher in concentration in WM. Content of inosinic acid(IMP) in WM(680.9mg/100g) was higher than that of DM(73.1mg/100g). The degradations of IMP proceeded very rapidly in DM. DM contained much higher trimethylamine oxide and trimethylamine than those of WM. The profile of combined amino acids in these samples were very similar, and main amino acids were Glu, Asp, Lys, Ala, Ile and Arg.