Ki-67 labeling index as a prognostic marker in advanced stomach cancer

Sang Hyuk Seo,Kwang Hee Kim,Sang Hoon Oh,Yunseon Choi,Ki Jung Ahn,Ji Young Lee,Sang Min Lee,박지선,Woo Gyeong Kim 대한외과학회 2019 Annals of Surgical Treatment and Research(ASRT) Vol.96 No.1

Purpose: Proliferation marker Ki-67 is widely used in cancer prognosis prediction. We tried to investigate the role of Ki-67 as a prognostic factor in stomach cancer after surgery in this study. Methods: We retrospectively evaluated 251 patients who underwent curative resection for gastric cancer from 2010 to 2015. In pathologic examination, Ki-67 labeling index was defined as the percentage of Ki-67 antigen positive cells. Prognostic significance of Ki-67 for gastric cancer was evaluated. Disease-free survival (DFS) was assessed as a primary end-point. Results: The median follow-up period was 28.0 months. Thirty-one patients (12.4%) showed Ki-67 labeling index (LI) lower than 25%. Sixty-eight patients (26.6%) showed recurrence during follow-up period. Recurrence was associated with Ki- 67 LI level (≤25%, P = 0.016), and lymph node metastasis status (P = 0.002). High Ki-67 LI level (>25%) was also related to p53 positivity (P < 0.001) and poorly cohesive type (P = 0.002). The 3-year DFS was 69.4%. Low Ki-67 LI level (≤25%) was related with low DFS (47.6% vs. 72.6%, P = 0.016). T stage (P < 0.001), N stage (P = 0.006), lymphovascular invasion (P = 0.010), and neuronal invasion (P = 0.001) also affected the DFS. In addition, T stage (P = 0.03) and Ki-67 LI (P = 0.035) were independent prognostic factors for DFS. In patients treated with adjuvant chemotherapy (n = 239, 93.4%), low Ki-67 (≤25%) was a poor prognostic factor for DFS (P = 0.013). Conclusion: Low Ki-67 LI predicts high rate of progression and low DFS of stomach cancer. Ki-67 LI can be a predictive marker in resected stomach cancer treated with surgery and adjuvant chemotherapy.

Cell proliferation and neuroblast differentiation in the rat dentate gyrus after intrathecal treatment with adipose-derived mesenchymal stem cells.

Choi, Jung Hoon,Chung, Jin Young,Yoo, Dae Young,Hwang, In Koo,Yoo, Ki-Yeon,Lee, Choong Hyun,Yan, Bing Chun,Ahn, Jin Ok,Youn, Hwa Young,Won, Moo-Ho Kluwer Academic/Plenum Publishers 2011 Cellular and molecular neurobiology Vol.31 No.8

<P>Mesenchymal stem cells (MSC) have emerged as a new therapeutic tool for a number of clinical applications, because they have multipotency and paracrine effects via various factors. In the present study, we investigated the effects of adipose-derived MSC (Ad-MSC) transplantation via intrathecal injection through the cisterna magna on cell proliferation and differentiation of endogenous stem cells in the hippocampal dentate gyrus (DG) using Ki-67 (a marker for proliferating cells), and doublecortin (DCX, a marker for neuroblasts). The transplanted Ad-MSC were detected in the meninges, not in the hippocampal parenchyma. However, the number of Ki-67-immunoreactive cells was significantly increased by 83% in the DG 2 days after single Ad-MSC injection, and by 67% at 23 days after repeated Ad-MSC treatment compared with that in the vehicle-treated group after Ad-MSC transplantation. On the other hand, the number of DCX-immunoreactive cells in the DG was not changed at 2 days after single Ad-MSC injection; however, it was significantly increased by 62% 9 days after single Ad-MSC injection. At 23 days after repeated Ad-MSC application, the number of DCX-immunoreactive cells was much more increased (223% of the vehicle-treated group). At this time point, DCX protein levels were also significantly increased compared with those in the vehicle-treated group. These results suggest that the intrathecal injection of Ad-MSC could enhance endogenous cell proliferation, and the repeated Ad-MSC injection could be more efficient for an enhancement of endogenous cell proliferation and differentiation in the brain.</P>

A Comparison of Lyophilized Amniotic Membrane with Cryopreserved Amniotic Membrane for the Reconstruction of Rabbit Corneal Epithelium

Ahn Jae-Il,Jang In-Keun,Lee Doo-Hoon,Seo Young-Kwon,Yoon Hee-Hoon,Shin Youn-Ho,Kim Jae-Chan,Song Kye-Yong,Lee Hee-Gu,Yang Eun-Kyung,Kim Ki-Ho,Park Jung-Keung The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.3

Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn's AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an ex-plantation method. The corneal epithelium could be reconstructed by culturing the third­passage corneal epithelial cells on the AM. A lyophilized amniotic membrane (LAM) has a higher rate of graft take, a longer shelf life, is easier to store, and safer, due to gamma irradiation, than a (AM. The corneal epithelium reconstructed on the LAM and (AM, supported by the two­Teflon rings, was similar to normal corneal epithelium. However, the advantages of the LAM over that of the (AM make the former more useful. The reconstruction model of the corneal epithelium, using AM, is considered as a good in vitro model for transplantation of cornel epithelium into patients with a severely damaged cornea.

Reconstruction of Rabbit Corneal Epithelium on Lyophilized Amniotic Membrane Using the Tilting Dynamic Culture Method

Ahn, Jae-Il,Lee, Doo-Hoon,Ryu, Yang-Hwan,Jang, In-Keun,Yoon, Mun-Young,Shin, Youn Ho,Seo, Young-Kwon,Yoon, Hee-Hoon,Kim, Jae-Chan,Song, Kye-Yong,Yang, Eun-Kyung,Kim, Ki-Ho,Park, Jung-Keug Blackwell Publishing Inc 2007 Artificial Organs Vol.31 No.9

<P>Abstract: </P><P>Rabbit corneal epithelium was reconstructed using tilting dynamic culture with a self-manufactured, amniotic membrane (AM) supporter and a lyophilized amniotic membrane (LAM). Rabbit corneal epithelial (RCE) cells were cultured and cryopreserved after isolation from the limbus. The second- and third-passage RCE cells were plated onto the epithelial side of the LAM of Ahn's AM supporter. Two days later, the air–liquid interface culture was maintained with third-passage RCE cells for 6 days and second-passage corneal epithelial cells for 9 days. The average viability of thawed RCE cells, assessed using trypan blue dye exclusion, was 77.42%. The reconstructed corneal epithelium was characterized by histological (hematoxylin and eosin) and immunohistochemical staining (proliferating cell nuclear antigen) for light microscopy, and by reverse transcriptase-polymerase chain reaction, glucose assay, and transmission electron microscopy. The basal layer of the reconstructed corneal epithelium was well formed, and the epithelium was tightly constructed due to the increase in cell proliferation and differentiation caused by the tilting dynamic culture, as opposed to static culture. Tilting dynamic culture was useful for the reconstruction of the epithelium using easily damaged epithelial cells and resulted in more stratum cell layers. Moreover, cytokeratin (CK3) mRNA expression in tilting dynamic cultured third-passage RCE cells seeded onto AM was greater than in static cultured third-passage RCE cells. The morphology of the reconstructed corneal epithelium on LAM by tilting dynamic culture for 9 days resembled that of the skin epidermis. This was thought to be because the tilting dynamic culture not only accelerated the proliferation and differentiation of cells by physical or mechanical stimulation, but also ensured that the supply of medium was delivered to the basal cells more efficiently. Thus, the reconstruction of the corneal epithelium using LAM and tilting dynamic culture was considered to be a good in vitro model for autologous or allogeneic transplantation of corneal epithelium and skin epidermis in patients with damaged epithelia. </P>

영어 통제구문에 관한 연구

안종기,서정민 대불대학교 2001 論文集 Vol.7 No.1

The aim of this paper is to discuss the control construction like(1a, b, c) to explain the unexpressed subject of VP[inf]. In traditional framework it depended on Equi-NP deletion or minimal distance principle. In GB, chomsky(1980, 1981) suggested PRO to explain the unexpressed subject of VP[inf]. Manzini(1983) and Kim(1989) revised Chomsky's theory. But none of them can satisfy us to explain the unexpressed subject of Vp[inf]. I solved these problems by using the control theory of HPSG. The unexpressed subject of VP[inf] is token-identical to the INFLUENCED, COMMITTOR, or EXPERIENCER in the psoa, according as the control relation is of influence, commitment, or orientation, resperctively. That is, in(1c) the unexpressed subject of BP [inf] is token-identical to the INFLUENCED(mary), in (1d) the unexpressed subject to VP[inf] is token-identical to COMMITTOR(I) and in (1e) the unexpressed subject of VP[inf] is token-identical to EXPERIENCER(I). Furthermore I applied the control theory of HPSG to VP[inf] within noun phrases.

주정중독 입원환자의 다이설피람요법에 관한 예비연구

안정숙,강봉선,신정호,박기창 大韓神經精神醫學會 1989 신경정신의학 Vol.28 No.3

This study was conducted to investigate the effect of disulfiram on Korean alcoholics, and to evaluate the clinical applicability of disulfiram therapy as an aversive conditioning technique. In twelve alcoholic inpatients, the alcohol challenge test was carried out after loading 500mg/day of disulfiram for 7 days. During the alcohol challenge test, blood was sampled twice, before alcohol ingestion and at the maximal disulfiram-acetaldehyde reaction. Blood-alcohol concentration was measured by enzymatic analysis technique. The severity of aversive reaction was scaled subjectively by the patient. The results are as follows : 1) Side effects of disulfiram were mild and could be considered mostly manifestations of mental or physical predisposition of the alcoholic patient, and disappeared after reducing the dosage. 2) The severity of disulfiram-acetaldehyde reaction was negatively correlated to body weight under given loading dose of disulfiram and given challenging dose of alcohol. 3) Most of the disulfiram-acetaldehyde reaction was self-limited within 2 hours leaving no sequela. 4) Blood-alcohol concentration at the maximal aversive reaction was average 25.3mg/dl(range 21-49mg/dl), and not related to the subjective ratings of the aversive reaction. 5) On the telephone follow-up interview with spouses of the patients, 11 patients were identified, 8 of them(74%) had been abstinent for 3-16 months with only 5 patients on disulfriam maintenance. The other 3 patients had been abstinent for 6-15 months without disulfiram maintenance and regular follow-up after discharge. 6) Therapeutic success of abstinence was significantly correlated with the severity of aversive reaction in alcohol challenge test(p<0.1), but not with the total number of test(p>.05). From this study, we may conclude that disulfiram therapy under medical supervision is not so dangerous as we have concerned and we would suggest that the disulfiram therapy is applicable as an aversive conditioning technique.

Nuss 방법에 의한 소아 오목가슴 수술 후 정맥 통증자가조절법을 사용한 Fentanyl 과 Fentanyl-Ketorolac의 진통효과 비교

안기량,서은정,김천숙,강규식,유시현,정진헌,정지원 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.2

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석유화학공업 종사자의 유전독성 위험성 평가

안기원,박정선,서순팔,문재동,조진형 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.1

In order to evaluate the genotoxic hazard among workers potentially exposed to low level petrochemical substances, the analyses of micronuclei(MN) and sister chromatid exchanges(SCEs) in lymphocytes were performed in 46 male workers(as exposed group) and 46 nonexposed subjects(as control group). Mean frequencies of MN and SCEs(respectively, 12.9/1000 cells and 6.5/cell) in exposed group were very significantly higher than those(10.2/1000 cells and 5.4/cell) in control group. And there were also significant differences in mean frequencies of MN and SCEs adjusted for age, employment duration, smoking, and drinking between two groups. Median frequencies of MN and SCEs in exposed group were very significantly higher than those in control group. Frequencies of SCEs were higher in smokers than in non-smoker. Frequencies of MN in smokers, however, were similiar to those of non-smoker. Interaction between exposure and smoking on MN and SCEs induction was not observed. The results suggest that there is genotoxic hazard in high risk group like workers handling carcinogens in petrochemical plants and the analyses of MN and SCEs are useful biomarkers for the exposure to hazard substances even at the level below the exposure limit.

부분 치수절단술을 이용한 복잡 치관파절의 치료 : 증례보고

안병덕,김영재,장기택,이상훈,김종철,한세현,김정욱 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.2

혼합 치열기 및 초기 영구치열기의 소아,청소년에서 외상에 의한 치관 파절은 빈번하게 발생한다. 치관파절은 치수의 노출 유무에 따라 단순 치관파절,복잡 치관파절로 분류된다. 치수가 노출된 복잡 치관파절의 경우 치수에 대한 고려 및 처치가 필수적인데,초기 영구치열기에는 전치부 치근의 발육이 완전히 이루어지지 않았기 때문에 완성된 치근을 가지는 치아보다 치료가 복잡하다. 치료의 목적은 적절한 치수 치료를 통해 치근의 완성을 도모하는 것으로 이를 위해 직접 치수복조술,부분 치수절단술,치경부 치수절단술의 방법을 이용한다. 이 중 부분 치수절단술은 성공률도 높으며 치경부 치수를 보존하기 때문에 여러 장점을 지니는 치료방법이다. 복잡 치관파절을 주소로 내원한 환자에서 부분 치수절단술을 시행하여 양호한 결과를 나타내어 이를 보고하는 바이다. Traumatic crown fracture in mixed dentition or early permanent dentition is relatively common. Crown fracture is classified into simple or complicated crown fracture by the presence of pulp exposure. The condition of pulp must be considered in treatment of crown fractures with pulp exposure. Treatment of immature crown-fractured incisor with pulp exposure is more complex because of its incomplete root formation. Pulp capping, partial pulpotomy, cervical pulpotomy can be used for continuous root development in immature crown-fractured incisor with pulp exposure. The success rate of partial pulpotomy is very high and there are several advantages of partial pulpotomy because the cell-rich coronal pulp tissue is preserved. This paper reports 2 cases of crown-fractured permanent incisors with pulp exposure that had been treated by partial pulpotomy successfully.

이용고객에 의한 농산물 도매법인의 평가 : 광주지역 농산물 도매시장의 출하자 및 중도매인을 중심으로 With special emphasis on the producers and intermediary wholesalers of public agricultural products wholesale martek in Gwangju area

안상돈,기회성,김정주 建國大學校 自然科學硏究所 2004 建國自然科學硏究誌 Vol.15 No.-

Since the economic system of capitalism is a society established on the basis of efficiency and competition, the class of people either inefficient or falling behind in competition will gradually weed out. Such principles of the market economy will apply, without exception, to the basic nucleus of the distribution system of the agricultural products, and it is evident that the agricultural products wholesale market companies being managed comparatively inefficiently run the risk of weeding out by themselves. At this point of time when the distribution system of agricultural products becomes diversified and large-sized, and differentiated, the relatively inefficients elements of the agricultural products wholesale market companies may most probably endanger the wholesale market companies, the nucleus of the agricultural products distribution system. The producers and intermediary wholesalers being the main customers of the wholesale market companies, the problems resulting from the conflicts of interest between them are very difficult to compromise and it takes a great deal of efforts and time to satisfy the needs of the producers and the intermediary wholesalers. Nevertheless, it is the hard realities that amid such difficulties, the agricultural products wholesale market companies can not neglect to meet the desires of their main customers, the producers and intermediary wholesalers. Since, however, the wholesale market companies are business entities themselves, it is also evident that they will eventually weed out, if they go against the trend of the times wherein all business entities should pursue "customer emotion" beyond "customer satisfaction". It would, therefore, be very opportune to gauge how the producers and intermediary wholesalers, the two main customers of the wholesale market companies evaluate the use of the wholesale market companies, and how satisfied they are with the services offered by the wholesale market companies. At the same time, such research seems to provide the useful basic materials to establish the survival strategies of the wholesale market companies. The examination of the evaluation, by the producers and intermediary wholesalers, the two main customers of the wholesale market companies, of the use of the wholesale market companies shows that both the producers and the intermediary wholesalers have favorably evaluated the items on (1) improvement of the order of business transactions, (2) rationalization of the level of the market rates such as the commission for sale on consignment, loading and unloading charges and bounties (3) impartiality of auctions, (4) level of satisfaction with reliability and diversity of the information of business transactions, (5) efforts of the employees of the wholesale market companies for collection and sales of the goods and (6) level of satisfaction with the services offered by the employees of the wholesale market companies to main customers. At the same time, when asked whether the use of the wholesale market companies improved their business revenues, the producers (25.0%) and intermediary wholesalers (30.0%) evaluated negatively. Such negative results may very likely lead to threatening factors hampering the growth and development of the wholesale market companies. The above negative results may be even more conspicuous especially under the circumstances where the agricultural products distribution system gets diverse and large-sized and differentiated through revitalization of direct transactions between the producers and large-scale discount marts and the competitions among the companies engaged in the same category of industry are very keen. From the viewpoint of the survival strategy of the wholesale market companies, approaches should be so made as the efforts to improve on the earnings of the producers and wholesale market companies may precede. In the evaluation of the payment methods, most of the producers (90.0%) were affirmative, while intermediary wholesalers were negative (35.0%). However, in consideration of the characteristics of the wholesale market companies having the producers and intermediary wholesalers as their customers, it seems that the methods and procedures for the payments should be improved by all means. In particular, if the wholesale market companies should approach this matter from WIN-WIN strategy, it seems that this matter might be resolved more easily. The changes in the environments faced in management activities of enterprises are quickening and at the same time, the growth or weeding out of enterprises is also quickly determined. Amid such changes, the gauging and satisfying of enterprise desires as the survival strategy must be definitely an extremely important element. The wholesale market company being an business entity itself, should be keen to such changes, and needs to make strategic approaches to its customers, if it does not want to weed out amid such changes.