http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Khin Thida Myint,Chang Kil Kim,Mi Young Chung,Jae Suk Park,Ki Byung Lim,Jae Dong Chung 한국원예학회 2006 Horticulture, Environment, and Biotechnology Vol.47 No.6
An efficient regeneration system for in vitro propagation of Phalaenopsis via direct somatic embryogenesis was established using thin-sectioned leaf explants. Direct induction of somatic embryos was observed from the thin-sectioned leaf explants cultured on a half-strength Murashige and Skoog (MS) medium supplemented with 9.08 μM thidiazuron for 2 weeks in darkness followed by l6-h photoperiod with approximately 60 μ㏖ㆍm?¹ㆍs?¹ light intensity (cool white fluorescent illumination). Somatic embryos were transferred to a half-strength MS medium containing no plant growth regulators for further growth and development. Plants derived from somatic embryos were acclimated and successfully established in a greenhouse.
Optimization of efficient protocorm-like body (PLB) formation of Phalaenopsis and Dendrobium hybrids
Soe, Khaing Wah,Myint, Khin Thida,Naing, Aung Htay,Kim, Chang Kil 경북대학교 농업생명과학대학 2014 Current Research on Agriculture and Life Sciences Vol.32 No.4
Optimization of the protocorm-like body (PLB) formation of Phalaenopsis and Dendrobium hybrids was performed by determining the effects of plant growth regulators (PGRs) and different parts and division sizes of the PLB. For both genera, the base part was the best for the proliferation of PLBs, yielding the highest number of PLBs on a PGR-free medium for Phalaenopsis and medium containing 0.1μM NAA and 10.0μM BAP for Dendrobium. As regards the division size, four-division sections resulted in a higher PLB formation efficiency for Phalaenopsis, while two-division sections produced a higher PLB formation efficiency for Dendrobium. It is expected that these findings will be applicable to efficient PLB formation of other Phalaenopsis and Dendrobium orchids.
Optimization of efficient protocorm-like body (PLB) formation of Phalaenopsis and Dendrobium hybrids
Soe, Khaing Wah,Myint, Khin Thida,Naing, Aung Htay,Kim, Chang Kil Institute of Agricultural Science and Technology 2014 慶北大農學誌 Vol.32 No.4
Optimization of the protocorm-like body (PLB) formation of Phalaenopsis and Dendrobium hybrids was performed by determining the effects of plant growth regulators (PGRs) and different parts and division sizes of the PLB. For both genera, the base part was the best for the proliferation of PLBs, yielding the highest number of PLBs on a PGR-free medium for Phalaenopsis and medium containing $0.1{\mu}M$ NAA and $10.0{\mu}M$ BAP for Dendrobium. As regards the division size, four-division sections resulted in a higher PLB formation efficiency for Phalaenopsis, while two-division sections produced a higher PLB formation efficiency for Dendrobium. It is expected that these findings will be applicable to efficient PLB formation of other Phalaenopsis and Dendrobium orchids.
Micropropagation and Conservation of the Wild Medicinal Orchid, Coelogyne cristata
Aung Htay Naing,Khin Thida Myint,Yoon Jung Hwang,In Sook Park,Jae Dong Chung,Ki Byung Lim 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.2
Micropropagation of the wild medicinal orchid, Coelogyne cristata, was conducted through culture of shoot axis, intact seedlings and pseudobulb segments. The shoot axis cultured on half strength Murashige and Skoog (½ MS) medium with 0.44 ㎎?L<SUP>-1</SUP> thidiazuron (TDZ) gave the optimum shoot response and fresh weight. Higher concentrations of TDZ above the optimum level seemed to gradually increase inhibitory effects on shoot regeneration and plant growth. Intact seedlings showed maximum number of shoots on Murashige and Skoog (MS) medium with 1.0 ㎎?L<SUP>-1</SUP> naphthalene acetic acid (NAA) and 1.0 ㎎?L<SUP>-1</SUP> 6-benzylaminopurine (BA) combination, without activated charcoal (AC). In two types of pseudobulb segment (basal and terminal segments) culture, basal segments produced better shoot response in all treatments than that of terminal segments. Of different inoculum types, medium and growth regulators used in the present study, the culture of seedlings on MS media with 1.0 ㎎?L<SUP>-1</SUP> NAA and 1.0 ㎎?L<SUP>-1</SUP> BA combination without AC appears to have great potential for in vitro propagation of this extremely wild orchid. The obtained plantlets showed no genetic variation.