경상북도 농업기술원 이전을 위한 입지여건 분석

김원정,권오성,장경수,여준호 경북대학교 농업생명과학대학 2015 Current Research on Agriculture and Life Sciences Vol.33 No.1

기후변화, 농업용수, 대기오염 등의 환경변화에 민감한 연구단지의 특성상 환경 자원의 이용이 용이해야 하나 현 농업기술원은 농업연구를 위한 환경 및 생태계 유지가 힘든 실정이다. 또한 지속 가능한 농업, 6차 산업화와 같은 새로운 패러다임에 입각한 기반시설을 수립하고 공공서비스의 효율적인 공급을 위해서는 경상북도농업기술원의 이전이 불가피하다. 이에 따라 농업기술원 이전을 위한 입지이론 및 방법을 검토하고 입지여건 분석을 위한 입지선정 기준을 제시하는 것이 본 연구의 목적이다. 따라서, 먼저 공공시설 이전에 관한 입지이론에 대해 알아보았고, 입지선정을 위한 연구방법으로 후보지 여과방법을 사용하였다. 후보지 여과방법에는 요인별 점검법, 나뭇가지 모양분석법, 혼합형 분석법이 있는데 본 연구에서는 요인별 점검법을 바탕으로 하여 입지기준을 제시하였다. 이에 따라 입지선정 기준으로 경제성과 개발의 용의성, 접근성과 편의성, 농업여건, 토양여건, 인력자원 지원이 가능한 곳 그리고 연계성과 형평성이 고려되었다. Due to various environmental changes, the current location of the Gyeongsangbuk-do Agricultural Research & Extension Services needs to be changed to provide an appropriate environment and ecosystem for agricultural research and the efficient provision of public services. Accordingly, this study proposes a standard for selecting the new location. First, the location theory for the transfer of public facilities is investigated, and candidate filtration methods considered for the location selection. The candidate filtration methods include a component inspection, branch shape analysis, and mixed analysis. This study uses a component inspection, where the component criteria include the economic feasibility and ease of development, accessibility and convenience, agricultural conditions, soil conditions, connectivity, fairness, and human resource-richness of the region.

Effect of Different Fertilizer Ratio and Planting Dates on Growth and Acanthoside D content of Acanthopanax divaricatus and Acanthopanax koreanum

Jung Jong Lee,Sang Hyun Lee,Jae Sang Park,Chung Berm Park,Young Sub Ahn,Sang Chul Lee 경북대학교 농업생명과학대학 2013 Current Research on Agriculture and Life Sciences Vol.31 No.2

The cultivation methods of Acanthopanax in Korea need to be optimized. Hence, this study investigated the effect of different fertilizer ratios and planting dates on the growth and acanthoside D content of two (2) Acanthopanax species. The current recommended fertilizer rate of 10.5-8.5-8.5 kg/ha- (N-P2O5-K2O, respectively) produced the best plant growth of Acanthopanax. For the first year, the acanthoside D content resulting from the 2P (2x phosphate) rate was higher than that from the other fertilizer ratios, yet there were no significant differences resulting from the various treatments for either Acanthopanax divaricatus or Acanthopanax koreanum. Similarly, for the second year, there were no significant differences in the acanthoside D content resulting from the various fertilizer ratios, although for both species the acanthoside D content resulting from the 2P rate was slightly higher than that from the other treatments. Therefore, the results indicated that doubling the amount of phosphate increased the acanthoside D content. Plus, the optimum planting date with respect to growth and productivity for Acanthopanax divaricatus was identified as April 15. The cultivation methods of Acanthopanax in Korea need to be optimized. Hence, this study investigated the effect of different fertilizer ratios and planting dates on the growth and acanthoside D content of two (2) Acanthopanax species. The current recommended fertilizer rate of 10.5-8.5-8.5 kg/ha- (N-P2O5-K2O, respectively) produced the best plant growth of Acanthopanax. For the first year, the acanthoside D content resulting from the 2P (2x phosphate) rate was higher than that from the other fertilizer ratios, yet there were no significant differences resulting from the various treatments for either Acanthopanax divaricatus or Acanthopanax koreanum. Similarly, for the second year, there were no significant differences in the acanthoside D content resulting from the various fertilizer ratios, although for both species the acanthoside D content resulting from the 2P rate was slightly higher than that from the other treatments. Therefore, the results indicated that doubling the amount of phosphate increased the acanthoside D content. Plus, the optimum planting date with respect to growth and productivity for Acanthopanax divaricatus was identified as April 15. The cultivation methods of Acanthopanax in Korea need to be optimized. Hence, this study investigated the effect of different fertilizer ratios and planting dates on the growth and acanthoside D content of two (2) Acanthopanax species. The current recommended fertilizer rate of 10.5-8.5-8.5 kg/ha- (N-P2O5-K2O, respectively) produced the best plant growth of Acanthopanax. For the first year, the acanthoside D content resulting from the 2P (2x phosphate) rate was higher than that from the other fertilizer ratios, yet there were no significant differences resulting from the various treatments for either Acanthopanax divaricatus or Acanthopanax koreanum. Similarly, for the second year, there were no significant differences in the acanthoside D content resulting from the various fertilizer ratios, although for both species the acanthoside D content resulting from the 2P rate was slightly higher than that from the other treatments. Therefore, the results indicated that doubling the amount of phosphate increased the acanthoside D content. Plus, the optimum planting date with respect to growth and productivity for Acanthopanax divaricatus was identified as April 15. The cultivation methods of Acanthopanax in Korea need to be optimized. Hence, this study investigated the effect of different fertilizer ratios and planting dates on the growth and acanthoside D content of two (2) Acanthopanax species. The current recommended fertilizer rate of 10.5-8.5-8.5 kg/ha- (N-P2O5-K2O, respectively) produced the best plant growth of Acanthopanax. For the first year, the acanthoside D content resulting from the 2P (2x phosphate) rate was higher than that from the other fertilizer ratios, yet there were no significant differences resulting from the various treatments for either Acanthopanax divaricatus or Acanthopanax koreanum. Similarly, for the second year, there were no significant differences in the acanthoside D content resulting from the various fertilizer ratios, although for both species the acanthoside D content resulting from the 2P rate was slightly higher than that from the other treatments. Therefore, the results indicated that doubling the amount of phosphate increased the acanthoside D content. Plus, the optimum planting date with respect to growth and productivity for Acanthopanax divaricatus was identified as April 15. The cultivation methods of Acanthopanax in Korea need to be optimized. Hence, this study investigated the effect of different fertilizer ratios and planting dates on the growth and acanthoside D content of two (2) Acanthopanax species. The current recommended fertilizer rate of 10.5-8.5-8.5 kg/ha- (N-P2O5-K2O, respectively) produced the best plant growth of Acanthopanax. For the first year, the acanthoside D content resulting from the 2P (2x phosphate) rate was higher than that from the other fertilizer ratios, yet there were no significant differences resulting from the various treatments for either Acanthopanax divaricatus or Acanthopanax koreanum. Similarly, for the second year, there were no significant differences in the acanthoside D content resulting from the various fertilizer ratios, although for both species the acanthoside D content resulting from the 2P rate was slightly higher than that from the other treatments. Therefore, the results indicated that doubling the amount of phosphate increased the acanthoside D content. Plus, the optimum planting date with respect to growth and productivity for Acanthopanax divaricatus was identified as April 15.

Identification of protease-resistant proteins from allergenic nuts using two- dimensional gel electrophoresis and mass spectrometry

Ilyn L. Santos,Ju-Young Lee,Yujin Youm,Jinkyu Lim 경북대학교 농업생명과학대학 2013 Current Research on Agriculture and Life Sciences Vol.31 No.2

Nuts are one of the most common sources of allergies in individuals of all ages. In order for a particular protein to render an allergic reaction, it must resist proteolytic digestion by intestinal enzymes. In this study, three well-known allergenic nuts, almonds, cashew nuts, and peanuts, were used as samples, and enzyme digestion with Bacillus protease and porcine pepsin was tested. A proteomic approach using two-dimensional gel electrophoresis and an MS/MS analysis was applied to visualize and identify the proteins that were resistant to enzyme digestion. Among the 150 protein spots tested, 42 proteins were assigned functions. Due to the lack of genomic databases, 41% of the identified proteins were grouped as hypothetical. However, 12% of them were well-known allergens, including AraH. The remainder were grouped as storage, enzymes, and binding proteins.

Effect of Soy Protein Diet on Mucosa Layer of Murine Small Intestine

Aeri Lee,Jinkyu Lim 경북대학교 농업생명과학대학 2014 Current Research on Agriculture and Life Sciences Vol.32 No.1

Soy and fermented soy are popular and recognized as a health food among Koreans. Since soy proteins are known to be protease resistant, even to pepsin and pancreatin, it is hypothesized that soy proteins may interact with the intestinal tract and trigger certain physiological reactions. To test this hypothesis, mice were fed diets supplemented with soy, Chunkukjang, or casein. The differentially expressed proteins were analyzed using 2-D gels and identified by peptide mass fingerprinting using mass spectrometry. The majority of the differentially expressed proteins could be functionally grouped into metabolic enzymes and calcium-binding proteins. The differential protein expression by the soy-fed groups was also verified based on a representative protein, tropomyosin, using a Western blotting analysis. In addition, the soy-fed groups exhibited a taller villi structure. Therefore, this study suggests that soy proteins can be an effective nutrient and physiological stimulant for the intestines.

GUS Expression Driven by Promoter of AtSAGT1 Gene Encoding a Salicylic Acid Glucosyltransferase 1 in Arabidopsis Plants

Pamella Marie Sendon,Jong-Beum Park,Soon-Ki Park,Jong Tae Song 경북대학교 농업생명과학대학 2013 Current Research on Agriculture and Life Sciences Vol.31 No.2

AtSAGT1 encodes a salicylic acid (SA) glucosyltransferase enzyme that catalyzes the formation of SA glucoside and SA glucose ester. Here, the AtSAGT1 gene expression patterns were determined in AtSAGT1 promoter::GUS transgenic Arabidopsis plants. As a result, the factors regulating the induction of AtSAGT1 were identified as pathogen defense response, wound response, exogenous application of SA, and jasmonic acid treatment.

Antioxidant and Oxidative DNA Damage Protection Potential of Methanol Extract of Red Tea Stem

Anil Kumar Yadav,Sun Chul Kang 경북대학교 농업생명과학대학 2013 Current Research on Agriculture and Life Sciences Vol.31 No.2

This study was undertaken to determine free radical scavenging capacity and oxidative DNA damage protecting activity of methanol extract of red tea stem. The extract was subjected to assess their antioxidant potential using various in vitro systems such as DPPH·, ABTS·+ , super oxide and nitric oxide free radicals and it exhibited IC50 values of 68.88 ± 1.1, 12.08 ± 0.65, 404.38 ± 1.6, 93.6 ± 2.7, µg/mL respectively. Red tea extract also showed ferric reducing ability (FRAP) with 2606.85 mmol Fe (II)/g of extract. Furthermore, Methanol extract of red tea stem showed significant DNA damage protecting activity in concentration dependent manner against H2O2+UV induced photolysis on pUC19 plasmid DNA. Results of this study showed that the methanol extract of Red Tea stem has strong antioxidant potential along oxidative DNA damage protecting capacity that would be the significant sources of natural antioxidants, which might be helpful in preventing the progress of various oxidative stress generated diseases. Further study is necessary for isolation and characterization of the active antioxidants, which may serve as a potential source of natural antioxidant.

Compare of Agriculture Character of Drought-Tolerant GM in Large GM Field

이현숙,김경민 경북대학교 농업생명과학대학 2013 Current Research on Agriculture and Life Sciences Vol.31 No.2

환경변화 중요성과 유전적 안전성은 최근에 증가추세의 형질전환 작물에 인식되고 있다. 본 실험은 건조저항성형질전환체 작물의 유전적인 안전성과 환경변이에 따른 농업적인 특성을 분석하였다. 내건성 형질전환체인CaMsrB2-8, 23 과 모품종인 ‘일미’ 및 일반품종을 대조구로GM필드에서 작물학적 생육특성을 조사하였다. 농업적인생육 특성에서 CaMsrB2-8, 23 계통은 모품종인 ‘일미’와년차간, 지역별 평균으로 표현형은 유사하였다. 수량에서 년도별, 지역별 차이는 있지만 통계적인 유의성은 없었다.현미의 미립특성에서 CaMsrB2-8, 23은 모품종인 ‘일미’와차이를 보이지 않았다. 현미의 화학적 성분 분석에서CaMsrB2- 2 계통의 전분과 단백질함량은 모품종인 ‘일미’보다 일반품종인 ‘일품’ 화학적 성분함량이 유사하였다.본 실험결과에서 내건성 형질전환체인 CaMsrB2-8, 23는GM 작물의 후대에서도 유전적인 안전성과 함께 수행될수 있을 것으로 사료된다. The significance of environment change and genetic safety has been recently recognized by many genetically modified (GM) plants. This study was to evaluate the safety of drought-tolerant rice and to identify the environment variance. The GM rice of drought-tolerant rice and four check cultivars were analyzed the data on agronomic characters and principal component in large-GM crop field. There was no significant difference in agronomic characters between the drought-tolerant rice and donor plant, ‘Ilmi’. Grain yield showed the standard deviation of the difference, did not significant statistically. Related to grain characters, grain appearance were similar to the drought-tolerant rice and donor plant, ‘Ilmi’. In Chemical characters, brown rice of the drought-tolerant rice and a donor plant, ‘Ilmi’ did difference in starch and protein, however, was similar as ‘'Ilpum’. These results indicated that drought-tolerant rice may perform to detect genetic safety in GM plants progeny.

물수요 중심 용수공급시스템 활용을 위한 국내 농업용수 공급체계 분석

이광야,최경숙 경북대학교 농업생명과학대학 2013 Current Research on Agriculture and Life Sciences Vol.31 No.2

This study analyzed agricultural water distribution systems for the utilization of water demand-oriented water supply systems. Three major TM/TC(telemeter/telecontrol) districts of agricultural water management were selected for analyzing the characteristics of the water distribution systems. In addition, the characteristics of the water supply systems for general water supply zones based on irrigation facilities were also investigated, along with the case of special water management during the drought season. As a result, high annual and monthly variations were observed for the water supply facilities, including the reservoirs and pumping stations. In particular, these variations were more obvious during the drought season, depending on the type of facility. The operations of the pumping stations and weirs were more sensitive to the stream levels than the reservoirs, and the smaller reservoirs were influenced more than the larger reservoirs. Therefore, a water-demand-oriented water supply system should consider the existing general practices of water management in the agricultural sector, and focus on achieving a laborsaving system rather than water conservation in the case of reservoirs. Equal water distribution from the start to the end point of irrigation channels could be an effective solution for managing pumping stations.

농용 무인 헬리콥터의 정지 비행시 편류제어 성능의 평가

구영모 경북대학교 농업생명과학대학 2013 Current Research on Agriculture and Life Sciences Vol.31 No.2

The precision aerial application of small farms, such as paddy, upland and orchard fields using agricultural unmanned helicopters became a new paradigm. The objective of this study was to evaluate the performance of a GPS module and algorithm, controlling drift of agricultural helicopter by the crosswind and maintaining the position for emergency landing. Purpose of the drift control, of which an algorithm works while hovering is related with the emergency sequence that coping with abnormal conditions of rotorcraft system. However, the inertial attitude control cannot detect a drifting motion of fuselage moving at the constant velocity, thus the crosswind takes the helicopter away from the landing position. Performance of the drift control module, based on the GPS that a hovering position did not deviate within 5m in diameter, were tested and evaluated. Initially, the reaction against a disturbing gust wind was sensitive, soon the helicopter maintained its locking position and azimuth within 5m in diameter. It was, however, difficult for the helicopter to recognize the swaying and nodding, the some deviation was expected due to the discrepancy characteristics of the GPS signal. The performance of the drift control proved the effectiveness of the module to maintain the position against an unintended drift during the emergency landing or hovering. 농용무인 헬리콥터는 벼농사는 물론 전작, 과수 등 소규모 필지의 정밀방제에 이용되고 있으며 농작업의 새로운 패러다임으로 자리 잡고 있다. 본 연구의목적은 농용헬리콥터의 비상시퀀스에서 정지비행 중 측풍에 의해서 비상착륙자리를 이탈하지 않고 위치를 유지하는 편류제어 모듈과 알고리즘의 성능을 평가하는데 있다편류제어의 목적은 비상착륙과 연동되어있는데, 비상조건에 도달하게 되면 호버링을 하면서 비상대처 알고리즘이 작동하게 된다. 그러나 관성 제어 는등속운동에서기체의 움직임을 감지하지 못하게 되고 측풍에 의하여 비상지점으로부터 편류를 하게 되어 착륙 목표지점으로부터 멀어질 수 있다.GPS 모듈을 기초로 개발한 편류제어모듈을 시험하였다.알고리즘 및 실효성을 고려하여 5 m 직경 내에 위치를벗어나지 않는지에 대한 기준을 적용하였다. 초기에는 2~4m/s의 측풍 교란에 대하여 과민하게 반응하였지만 이후5 m직경 내에서 위치를 벗어나지 않고 자세 및 요의 방향을 유지 하였다. 목도의 관찰에서는 전후좌우 흔들림을인지하기 어려운 정도이지만, 데이터에서 보인 편위는GPS 수신기의 특성에 기인하는 것으로 판단한다. 이와같은 편류제어는 비상착륙이나 호버링을 유지하려 할 때의도하지 않는 편류를 제어하는데 사용될 수 있다.

원격탐사 기술의 산림탄소 축적량 추정적용에 있어서의 도전

박주원 경북대학교 농업생명과학대학 2013 Current Research on Agriculture and Life Sciences Vol.31 No.2

The carbon-offset mechanism based on forest management has been recognized as a meaningful tool to sequestrate carbons already existing in the atmosphere. Thus, with an emphasis on the forest-originated carbon-offset mechanism, the accurate measurement of the carbon stock in forests has become important, as carbon credits should be issued proportionally with forest carbon stocks. Various remote sensing techniques have already been developed for measuring forest carbon stocks. Yet, despite the efficiency of remote sensing techniques, the final accuracy of their carbon stock estimations is disputable. Therefore, minimizing the uncertainty embedded in the application of remote sensing techniques is important to prevent questions over the carbon stock evaluation for issuing carbon credits. Accordingly, this study reviews the overall procedures of carbon stock evaluation-related remote sensing techniques and identifies the problematic technical issues when measuring the carbon stock. The procedures are sub-divided into four stages: the characteristics of the remote sensing sensor, data preparation, data analysis, and evaluation. Depending on the choice of technique, there are many disputable issues in each stage, resulting in quite different results for the final carbon stock evaluation. Thus, the establishment of detailed standards for each stageis urgently needed. From a policy-making perspective, the top priority should be given to establishinga standard sampling technique and enhancing the statistical analysis tools.