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Naing Aung Htay,Soe May Thu,Win Nay Myo,강현희,정미영,김창길 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.6
We investigated whether ethylene is involved in the stem-bending mechanism of the two diferent gerberas [long-lived cultivar ‘Mary Queen’ and short-lived cultivar ‘Harmony’] by pretreating the stems with an ethylene-releasing compound (Ethephon), an ethylene-action inhibitor [silver thiosulfate (STS)], or Ethephon in combination with STS (Ethephon+STS), using distilled water as the control. In the cv. ‘Mary Queen,’ the control stems did not show stem bending, while the treatment groups showed bending at 6 and 9 days after treatment (DAT). In the cv. ‘Harmony,’ the bending rate observed in the Ethephon-treated stems was lower than the other treatments at 9 DAT. However, a lower bending rate in the Ethephon group relative to the other treatments is unlikely to be associated with ethylene action, because the bending rate observed in the Ethephon+STS group was signifcantly higher than the STS group. In addition, the bending rates of both cultivars were not associated with lignin contents or the expression of lignin biosynthesis genes in the stems. As it is complicated to conclude the role of Ethephon in stem bending of the gerberas, these results suggest that the use of Ethephon to delay stem bending in cut gerberas is not warranted
Optimization of Embryogenic Callus Induction and Plant Regeneration in Orchid Coelogyne cristata
Naing, Aung Htay,Lim, Ki-Byung Korean Society of Horticultural Science 2011 원예과학기술지 Vol.29 No.3
An efficient protocol was established for high frequency somatic embryogenesis through a callus culture of Coelogyne cristata. The best frequency of callusing was obtained from a PLB segment (3-5 mm) cultured on MS medium supplemented with coconut water (CW) and a combination of both 3 $mg{\cdot}L^{-1}$ of 2,4-D and BA. When the calli were sub-cultured on the MS medium without any PGRs, the average number of somatic embryos were higher than those with PGRs treatment. NAA is the most critical factor among PGRs, which dramatically hindered for the formation of a somatic embryo. The efficacy of the addition of coconut powder (CP) for somatic embryogenesis was almost the same in all treatments. However, the number of somatic embryos formed distinctly depended on age of the callus. The somatic embryos converted into healthy plants with well-developed shoots on the same medium. Plantlets showed the best responses of root and shoot growth when transferred to $\frac{1}{2}$ MS medium containing 1.5 $g{\cdot}L^{-1}$ of activated charcoal. All plants with above 3.0-cm-high were successfully acclimatized in the greenhouse.
Naing, Aung Htay,Park, Da Young,Park, Kyeung Il,Kim, Chang Kil SpringerOpen 2018 3 Biotech Vol.8 No.9
<P>We investigated the expression of anthocyanin structural genes and transcription factors (TFs) associated with varying anthocyanin content during different developmental stages (S1-S4) of the gerbera cultivars Nathasha' and 'Rosalin'. Accumulation of anthocyanin started at S1 and reached a maximum at S3 in both cultivars. Enhancement of anthocyanin content in Nathasha' was associated with upregulation of ANS and MYB10, whereas in 'Rosalin', upregulation was associated with CHS1, MYB10, and MYC1 . Low-temperature exposure (6 degrees C) enhanced anthocyanin content to a greater extent than that at 22 degrees C via stronger upregulation of CHS1 and MYB10 in Nathasha' and CHS1 in 'Rosalin', irrespective of flower developmental stage. However, differences in anthocyanin content between the two cultivars were found to be influenced by the expression levels of all structural genes and TFs, irrespective of flower developmental stage and temperature conditions. We suggest that differences in the regulation mechanisms of anthocyanin biosynthesis and coloration pattern between Nathasha' and 'Rosalin' are related to differences in the expression patterns of structural genes and TFs; however, further functional studies of the key genes in anthocyanin biosynthesis are needed.</P>
Naing, Aung Htay,Kim, Chang Kil Institute of Agricultural Science and Technology 2014 慶北大農學誌 Vol.32 No.4
This is the first report describing the seasonal conditions affecting shoot regeneration by the chrysanthemum cv. Baeksun. The shoot regeneration from petal explants was found to be more favorable from September to December, reaching the highest values in December. In addition, the quality of the shoots was also influenced according to the season of the explant collection, where healthy and uniform plants were derived from the explants collected in December. Choosing the proper season for explant collection affected the successive plant growth parameters (i.e., plant height and fresh weight). Thus, the current results strongly suggest that season plays an important role in plant tissue culturing, which is an essential tool for micropropagation and Agro-bacterium-mediated genetic transformation studies.