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Isolation and characterization of acid-soluble bluefin tuna (Thunnus orientalis) skin collagen
Tanaka, Teruyoshi,Takahashi, Kenji,Tsubaki, Kazufumi,Hirata, Maika,Yamamoto, Keiko,Biswas, Amal,Moriyama, Tatsuya,Kawamura, Yukio The Korean Society of Fisheries and Aquatic Scienc 2018 Fisheries and Aquatic Sciences Vol.21 No.4
In this study, we isolated and characterized the acid-soluble skin collagen of Pacific bluefin tuna (PBT, Thunnus orientalis). The PBT skin collagen was composed of two ${\alpha}$ chains (${\alpha}1$ and ${\alpha}2$) and one ${\beta}$ chain. The denaturation temperature of PBT collagen was low although it was rich in proline and hydroxyproline. The primary structure of PBT skin collagen was almost identical to that of calf and salmon skin collagen; however, it differed with respect to the epitope recognition of the antibody against salmon type I collagen. These results suggest that the primary structure of skin collagen was highly conserved among animal species, although partial sequences that included the epitope structure differed among collagens.
Isolation and characterization of acid-soluble bluefin tuna (Thunnus orientalis) skin collagen
( Teruyoshi Tanaka ),( Kenji Takahashi ),( Kazufumi Tsubaki ),( Maika Hirata ),( Keiko Yamamoto ),( Amal Biswas ),( Tatsuya Moriyama ),( Yukio Kawamura ) 한국수산과학회(구 한국수산학회) 2018 Fisheries and Aquatic Sciences Vol.21 No.2
In this study, we isolated and characterized the acid-soluble skin collagen of Pacific bluefin tuna (PBT, Thunnus orientalis). The PBT skin collagen was composed of two α chains (α1 and α2) and one β chain. The denaturation temperature of PBT collagen was low although it was rich in proline and hydroxyproline. The primary structure of PBT skin collagen was almost identical to that of calf and salmon skin collagen; however, it differed with respect to the epitope recognition of the antibody against salmon type I collagen. These results suggest that the primary structure of skin collagen was highly conserved among animal species, although partial sequences that included the epitope structure differed among collagens.
Yasuhiro Ito,Takuichi Sato,Keiko Yamaki,Gen Mayanagi,Kazuhiro Hashimoto,Hidetoshi Shimauchi,Nobuhiro Takahashi 한국미생물학회 2012 The journal of microbiology Vol.50 No.1
This study aimed to profile the microflora in infected root canals before and after root canal treatment using cultureindependent methods. Six infected root canals in singlerooted teeth with periapical lesions from five subjects were included. Quantification of total bacteria was performed by real-time PCR with primers targeting 16S rRNA genes. PCR products with universal 16S rRNA gene primers were cloned and partially sequenced, and bacterial identification at the species level was performed by comparative analysis with the GenBank database. The concentration of extracted DNA before treatment was higher than that after root canal treatment, although the difference was not statistically significant. Sequence analysis revealed that oral bacteria such as Fusobacterium, Streptococcus, Olsenella, and Pseudoramibacter detected in cases before root canal treatment disappeared after treatment. These results suggest that the root canal microflora are distinct before and after root canal treatment, and that treatment changes the microflora in both quantity and quality.
Essential protocols for in vitro evaluation of nanoparticle
Hitoshi Iwahashi,Masanori Horie,Keiko Nishio,Shigehisa Endoh,Haruhisa Kato,Katsuhide Fujita,Shinichi Kinugasa,Arisa Miyauchi,Ayako Nakamura,Junko Takahashi,Etsuo Niki,Yasukazu Yoshida,Junko Nakanishi 한국환경독성학회 2010 한국독성학회 심포지움 및 학술발표회 Vol.2010 No.11