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Shang, Ke,Amna, Touseef,Amina, Musarat,Al-Musayeib, Nawal M.,Al-Deyab, Salem S.,Hwang, Inho Medknow PublicationsMedia Pvt Ltd 2017 Pharmacognosy magazine Vol.13 No.49
<P><B>Background:</B></P><P>ellular damage initiated by reactive oxygen species (ROS) is the main cause of numerous severe diseases and therefore for this reason, the natural antioxidants have note worthy significance in human health. Capsaicin possesses noteworthy analgesic and anti-inflammatory properties. It also possesses healing effects for treatment of arthritis, diabetic neuropathy, gastric lesions, and cardiac excitability that is why it is incorporated in creams and gels.</P><P><B>Objective:</B></P><P>The present study was carried out to estimate the <I>in vitro</I> antioxidant and ROS scavenging activities of capsaicin against muscle precursor cells. Till date, no investigation has been carried out to study the effect of capsaicin on myoblasts.</P><P><B>Materials and methods:</B></P><P>Herein, the cytotoxicity was induced by endotoxin lipopolysaccharide (LPS) to analyze the effect of capsaicin on LPS induced inflammation and apoptosis on muscle cells. To find out the toxicity of endotoxin, myoblasts were exposed to different concentrations of LPS, viability and morphology was checkedby the means of CCK-8 test and microscopy, respectively. Apoptotic cell death was examined by fluorescence staining. Additionally, LPS-induced apoptosis was determined by mRNAexpression of calpain, caspase-3 and tumor necrosisfactor alpha (TNF-α), and were quantified by qRT-PCR.</P><P><B>Results:</B></P><P>The outcome of the presentstudy demonstrated that LPS stimulation generatestoxicity in dose-dependent manner. Pre-treatmentof myoblasts with capsaicin can considerably alleviate LPS-induced inflammation.</P><P><B>Conclusion:</B></P><P>In conclusion, this study indicates that dietetic supplementation of capsicum may help to alleviate/reduce the inflammatory effects and is therefore potent source of natural antioxidant agent which can be utilized to control muscle related diseases, such as myotube atrophy.</P><P><B>SUMMARY</B></P><P><P>In the present study cytotoxicity was induced by LPS to analyze the effect of capsaicin on LPS induced inflammation and apoptosis on muscle cells.</P><P>The results of this investigation demonstrated that LPS stimulation generates toxicity in dose dependent manner. Pre-treatment of myoblasts with capsaicin can considerably reduce LPS induced inflammation.</P><P>It has been concluded on the basis of results that the dietetic supplementation of capsicum may help to minimize inflammatory effects and are potent sources of natural antioxidants which can be utilized to control muscle related diseases such as atrophy.</P></P> >[FIG OMISSION]</BR><P><B>Abbreviation used:</B> AMP: Adenosine monophosphate, AO/EB: Acridine orange / Ethidium bromide, ATL: T-cell leukemi, CAP: Capsaicin, CCK-8: Cell counting Kit-8, CLSM: Laser Scanning Microscopy, DCF-DA: 2’, 7’-dichlorofluorescein diacetate, DMEM: Dulbecco’s modified Eagle’s medium, DPPH: α, α-diphenyl-β-picrylhydrazyl, FBS: Fetal bovine serum, KA: Kainic acid, LPS: Lipopolysaccharide, MDA: Malondialdehyde, NF-κB: Nuclear factor kgene binding, PBS: Phosphate buffer saline, pNA: p-nitroanilide, RNW: RNase free water, ROS: Reactive oxygen species, TNF-α: Tumor necrosis factor alpha, TRPV1: Transient receptor potential vanilloid 1</P>
Ke Ding,Ke Shang,Zu-Hua Yu,Chuan Yu,Yan-Yan Jia,Lei He,Cheng-Shui Liao,Jing Li,Chun-Jie Zhang,Yin-Ju Li,Ting-Cai Wu,Xiang-chao Cheng 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.2
Newcastle disease virus (NDV) and Salmonella Pullorum have significant damaging effects on the poultry industry, but no previous vaccinecan protect poultry effectively. In this study, a recombinant-attenuated S. Pullorum strain secreting the NDV hemagglutinin-neuraminidase(HN) protein, C79-13ΔcrpΔasd (pYA-HN), was constructed by using the suicide plasmid pREasd-mediated bacteria homologousrecombination method to form a new bivalent vaccine candidate against Newcastle disease (ND) and S. Pullorum disease (PD). The effectof this vaccine candidate was compared with those of the NDV LaSota and C79-13ΔcrpΔasd (pYA) strains. The serum hemagglutinationinhibition antibody titers, serum immunoglobulin G (IgG) antibodies, secretory IgA, and stimulation index in lymphocyte proliferation wereincreased significantly more (p < 0.01) in chickens inoculated with C79-13ΔcrpΔasd (pYA-HN) than with C79-13ΔcrpΔasd (pYA) but werenot significantly increased compared with the chickens immunized with the LaSota live vaccine (p > 0.05). Moreover, the novel strain provides60% and 80% protective efficacy against the NDV virulent strain F48E9 and the S. Pullorum virulent strain C79-13. In summary, in this study,a recombinant-attenuated S. Pullorum strain secreting NDV HN protein was constructed. The generation of the S. Pullorum C79-13ΔcrpΔasd(pYA-HN) strain provides a foundation for the development of an effective living-vector double vaccine against ND and PD.
Shang, Ke,Wei, Bai,Jang, Hyung-Kwan,Kang, Min Elsevier 2019 Food control Vol.100 No.-
<P><B>Abstract</B></P> <P>The present study investigated the distribution, antimicrobial susceptibility profiles, serotypes and genotypes of <I>Salmonella</I> isolates from a slaughterhouse (including lairage and slaughter line) and its downstream retail markets to determine the main sources of <I>Salmonella</I> contamination in retail chicken meat. Positive identification rates in the slaughterhouse and retail markets were 9.5% (81/852) and 41.8% (69/165), respectively. Among the <I>Salmonella</I> serovars identified, <I>S</I>. Albany was the most prevalent but other serovars including <I>S</I>. Montevideo and <I>S</I>. Virchow were also present prominently. Antimicrobial susceptibility testing revealed that the highest antimicrobial resistance rate was against nalidixic acid (139/150, 92.7%), that 56.7% (85/150) isolates were multidrug-resistant (MDR), and that the resistance profile of 20.7% (31/150) isolates was ACSSuT (ampicillin, chloramphenicol, streptomycin, sulfonamides and tetracycline). Ten virulence genes were detected in 69 isolates from retail chicken meat; the <I>Salmonella</I> pathogenicity island genes <I>inv</I>A, <I>hil</I>A, <I>sip</I>A, <I>sop</I>B, <I>sop</I>D, <I>ssr</I>A, and <I>ssa</I>R were present in most of the isolates (94.2%–100%). Based on analysis of pulsed-field gel electrophoresis (PFGE) results, our findings indicated that MDR isolates could be transmitted from slaughterhouse to its downstream retail markets. In addition, the present study indicates three possible routes of <I>Salmonella</I> contamination in retail chicken meat, including initial presence, cross-contamination from carrier broilers slaughtered on the same day, and contamination from resident flora in slaughterhouse, with the last route being the most common in the present study.</P> <P><B>Highlights</B></P> <P> <UL> <LI> AMR and genotype of <I>Salmonella</I> from slaughterhouse and markets were conducted. </LI> <LI> Frequent cross-contamination in slaughterhouse has been identified. </LI> <LI> The major causative contamination step in slaughter-line has been confirmed. </LI> <LI> Frequent propagation of MDR <I>Salmonella</I> from slaughterhouse to market has been found. </LI> <LI> Three routes of MDR <I>Salmonella</I> contamination in retail meat have been confirmed. </LI> </UL> </P>
Shang-Hao Liu,Chen-Feng Kuan,Cing–Yu Ke,Ming-Yuan Shen,Chin-Lung Chiang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.127 No.-
In this study, chitosan (CS) from fish waste was used to prepare bio-based environmentally friendly flameretardants. After the hydroxyl group (OH) of CS and the ammonium group (NH4 + ) of ammoniumpolyphosphate (APP) underwent reactions, they were filtered and dried to obtain CS-APP. The aminogroup of CS-APP then reacted with the epoxy group of 4,40-methylenebis(N,N-diglycidylaniline) (NDY)to form CS-APP-NDY. Isophorone diisocyanate, polyol, and 3-aminopropyltriethoxysilane reacted to formsilicon polyurethane. CS-APP-NDY was then mixed with Si-PU to prepare polymer composites. To determinethe structure, thermal properties, flame retardancy, and mechanical properties of the composites,Fourier-transform infrared spectroscopy, thermogravimetric analysis, limiting oxygen index (LOI), conecalorimetry, UL-94, thermal analysis-FTIR (TA-FTIR) spectroscopy, universal machine testing, scanningelectron microscopy, X-ray photoelectron spectroscopy, and Raman spectroscopy were performed. TheTGA results revealed that, after the addition of CS-APP-NDY, char yield increased from 0.5 wt% to 25.8wt%, and the thermal stability of pristine PU also improved. In addition, the LOI and UL-94 results indicatedthat, after the addition of CS-APP-NDY, the LOI increased from 18.2% to 26.3%, and the UL-94 levelimproved from ‘‘Fail” to V-1. Overall, these results indicated that the addition of CS-APP-NDY to pristinePU increased its flame-retarding performance.
Event-triggered Consensus Control of Nonlinear Multi-agent Systems based on First-order Hold
Yu Shang,Cheng-Lin Liu,Ke-Cai Cao 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.4
In this paper, consensus problem is studied for the first-order nonlinear multi-agent systems under directed topology, and a novel event-triggered consensus protocol is proposed. Different from the existing results with general zero-order hold, the first-order hold is adopted to construct the event-triggered control signal so as toreduce the triggering rate. By using Lyapunov stability method and matrix theory, a sufficient consensus conditionis derived for the agents to reach the asymptotic consensus, and Zeno-behavior can be avoided. Finally, a numericalexample is presented to demonstrate the effectiveness of our proposed protocol.