Anti-tumor activity of retinoic acid-incorporated glycol chitosan nanoparticles

Cy-Hyun Kim,Do-Hyung Kim,Hye-Myung Lee,Tae-Won Kwak,Young-IL Jeong,Chung-Wook Chung,Dae-Hwan Kang 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

Nanoparticles have been extensively investigated for targeted delivery of anticancer drug. Especially, chitosan based nanoparticles is believed as a promising carriers for cancer chemotherapy and imaging. Due to superior biocompatibility, non-cytotoxic, and biologically active properties, chitosan is frequently used as a biomedical materials and drug carriers. Furthermore, chitosan is regarded as an ideal vehicle for delivery of anionic drug or DNA drug due to its positive ionic proeprties. Chitosan is used to make polyion complexes with anionic drug such as all-trans retinoic acid (RA) and these ionic complexes can form nanoparticles in aqueous media We prepared RA-incorporated glycol chitosan (GC) nanoparticles by simple mixing RA into the GC aqueous solution through ion complex formation between RA and GC. Particle sizes of RA-incorporated GC nanoparticles were approximately 300~500 nm. Lyophilized nanoparticles were simply reconstituted into aqueous solution in spite of absence of cryoprotectants. RA-incorporated GC nanoparticles showed similar cytotoxicity against cholangiocarcinoma cells. Furthermore, RA-incorporated GC nanoparticles showed enhanced anti-invasive capacity and anti-migration capacity against HuCC-T1 cholangiocarcinoma cells. We suggest that RA-incorporated GC nanoparticles are promising vehicles for antitumor drug delivery.

자궁의 점액성 평활근육종 1 예

강문자,김종우,이신애,이지연,원찬연,편도진,이종은 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.12

Myxoid Leiomyosarcoma of uterus is a very rare malignant tumor and was first in 1982 by King et al. Although in general, leiomyosarcoma shows prognosis according to its mitotic count, this tumor is characterized by low mitotic count without atypia, but taking subsequent malignant course with frequent metastasis and recurrence. We experienced a case of myxoid leiomyosarcoma of uterus in a 42-year old woman with low abdominal discomfort and present this with brief review of literatures.

Synergistic effects of 5-aminolevulinic acid based photodynamic therapy and celecoxib via oxidative stress in human cholangiocarcinoma cells

Kim, Cy Hyun,Chung, Chung-Wook,Lee, Hye Myeong,Kim, Do Hyung,Kwak, Tae Won,Jeong, Young-IL,Kang, Dae Hwan Dove Medical Press 2013 International journal of nanomedicine Vol.8 No.-

<P>5-Aminolevulinic acid (ALA)-based photodynamic therapy (PDT) has the potential to kill cancer cells via apoptotic or necrotic signals that are dependent on the generation of intracellular reactive oxygen species (ROS). Celecoxib is an anti-inflammatory drug that induces intracellular ROS generation. We investigated whether the combined application of celecoxib and ALA-PDT improved the efficacy of PDT in human cholangiocarcinoma cells and in tumor bearing mice. In vitro, combined treatment of celecoxib and ALA-PDT increased phototoxicity and intracellular ROS levels after irradiation with 0.75 J/cm<SUP>2</SUP> when compared to ALA-PDT alone. Even though ROS levels increased with 0.25 J/cm<SUP>2</SUP> of irradiation, it did not influence phototoxicity. When heme oxygenase-1, a defensive protein induced by oxidative stress, was inhibited in the combined treatment group, phototoxicity was increased at both 0.25 J/cm<SUP>2</SUP> and 0.75 J/cm<SUP>2</SUP> of irradiation. We identified the combined effect of ALA-PDT and celecoxib through the increase of oxidative stress such as ROS. In vivo, about 40% tumor growth inhibition was observed with combined application of ALA-PDT and celecoxib when compared to ALA-PDT alone. The combined application of ALA-PDT and celecoxib could be an effective therapy for human cholangiocarcinoma. Moreover, use of a heme oxygenase-1 inhibitor with PDT could play an important role for management of various tumors involving oxidative stress.</P>

Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells

Kim, Cy Hyun,Chung, Chung-Wook,Choi, Kyung Ha,Yoo, Jin-Ju,Kim, Do Hyung,Jeong, Young-IL,Kang, Dae Hwan Dove Medical Press 2011 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.6 No.-

<P>Cancer cells have been reported to exhibit an enhanced capacity for protoporphyrin IX (PpIX) synthesis facilitated by the administration of 5-aminolevulinic acid (ALA). We investigated the effect of ALA-based photodynamic therapy (PDT) on human cholangiocarcinoma cells (HuCC-T1). Since protoporphyrin IX (PpIX), a metabolite of ALA, can produce reactive oxygen species (ROS) under irradiation and then induce phototoxicity, ALA-based PDT is a promising candidate for the treatment of cholangiocarcinoma. When various concentrations of ALA (0.05–2 mM) were used to treat HuCC-T1 cells for 6 or 24 hours, the intracellular PpIX level increased according to the ALA concentration and treatment time. Furthermore, an increased amount of PpIX in HuCC-T1 cells induced increased production of ROS by irradiation, resulting in increased phototoxicity.</P>

제왕절개술률의 현황과 질 지표로서의 의의

박중신,강철환,김창엽 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.9

With the sharply increasing practice of cesarean section(C/S), formerly known as a landmark of developing medical technology, appropriateness of the procedure has been widely inquired into. Appropriateness of a specific procedure could be an indicator for quality, at the individual or organizational level. Cesarean section rate is regarded as a sensitive indicator reflecting clinical quality at the hospital level. We are interested in the validity and significance of C/S rate as a quality indicator in Korean hospitals, in addition to the current status of the practice. This study was carried out retrospectively based on the clinical records of cesarean sections in 32 hospitals nationwide from March to May 1996. Standardized survey format was distributed, and reviews were performed by volunteer surveyors from each hospital after two sessions of pre-survey education. The results were as follows : 1. There were 13,241 deliveries with 4,599 cases of cesarean sections, giving an incidence of 38.5%. This result shows the incidence of cesarean section was 1.6 times higher than U.S. and about four times higher than European countries. 2. The monthly incidence of cesarean section was not variable, but it shows great differences among the hospitals. 3. The incidences of cesarean sections were variable among different age groups with marked higher rate in older one. 4. Most of deliveries were with gestational period from 37 to 44 weeks(91%), in which C/S rate was 39%. For pregnancies with less than or equal to 32 weeks, the rate was 21% and for more than or equal to 45 weeks the rate was 25%. 5. The most frequent indication of cesarean birth was previous cesarean section(37.7%), followed by cephalopelvic disproportion and dystocia(24.4%). 6. There were no statistical differences in cesarean section rates by hospital variables such as ownership, teaching status, proportion of specialists, number of physicians per bed, number of nurses per bed, regional location, and number of beds. Exceptionally, hospitals with independent obstetrics department, separated from gynecology, showed significant lower rate than hospitals with conventional obstetrics-gynecology department. We could confirm higher C/S rate than any other countries. However, C/S rate, not significantly different among hospitals with variable quality level in structural aspect, was not acceptable as a sensitive indicator for clinical quality at the hospital level. Different rates between comparable organizational settings, sometimes indirectly related to the clinical quality, suggested that more studies focused on other aspects of quality than structure should be needed.

절박유산 환자에서 hCG의 외부투여가 내인성 스테로이드 호르몬 값에 미치는 영향

강병철(BC Kang),박병호(BH Park),김기원(KW Kim),이진우(JW Lee),김창이(CY Kim) 대한산부인과학회 1981 Obstetrics & Gynecology Science Vol.24 No.11

1. 임신 제 6-10주의 고위험 절박유산 20예중 정상임신으로 이행한 12예에 있어서의 혈정 estradiol-17B의 hCG투여전 기초수준은 1143.3±455.67 pg/ml이었으며 최초의 hCG 5000 I.U 투여후 1시간에 1996.2±724.01 pg/ml로 증가하였으며 이는 기초수준의 187.1±61.84%에 해 당하였다. 2. 이들 12예에 있어서 최초의 hCG투여후 제 5 및 제10일의 혈중 estradiol-17B는 각각 1440.3±571.22 pg/ml 및 1793.0±512.6pg/ml로 증가하여 각각 기초수준의 132.9±41.22% 및 172.9±57.63%에 해당하였다. 3. 이들 12예에 있어서 혈중 progesterone의 hCG투여전 기초수준은 38.9+-20.58 ng/ml이었으 며 최초의 hCG투여후 1, 2 및 3시간에는 유의한 증가를 볼 수 없었다. 4. 이들 12예에 있어서 최초의 hCG투여후 제 5일 및 제 10일의 혈중 progesterone은 각각 49.0±17.31 ng/ml 및 61.6±20.31 ng/ml로 증가하여 각각 기초수준의 142.5±44.63% 및 182.5±68.08%에 해당하였다. 5. 임신 제 11-15 및 16-20주에 잇어서는 임신주수가 증가하고 투여전 기초수준이 높아질수 록 최초의 hCG투여후 1,2 및 3시간과 제 5, 제 10일의 혈정 estradiol-17B 및 progesterone 의 증가폭은 점점 감소하여 통계적 의의를 상실하였다. 이상의 관찰로 보아 절박유산환자에서 투여한 hCG에 대하여 estrogen은 민감한 반응을 즉 시 보이나 progesterone은 서서히 반응하여 이는 주로 초기 임신에서 더 현저하고 임신주수 가 증가할수록 미약한 반응을 나타냄을 측정할 수 있었다. 1. Serum estradiol-17B responsed immediatly to the administered hCG ; basal value of 1143±455.67 pg/ml increased to 1996.2±724.01 pg/ml corresponding to 187.1±61.84% of basal level 1 hour after first administraion of hCG in the group of 6-10 weeks gestation 2. The level of serum estradiol-17B on the 5 and 10 days after first administraion increased from the basal level to 1440.3±571.22 pg/ml and 1793.0+-512.6 pg/ml corresponding to 132.9±41.22% and 172.9±57.63% of basal value respectively. 3. Serum progesterone did not revealed such as early response 1 hour after administration of hCG as estradiol-17B and no significant increase was noted even 3 hours after the first administration of hCG. 4. The level of serum progesterone on the 5 and 10 days after the first administration of hCG increased from the basal value of 38.9+-20.58 , to 49.0±17.31ng/ml and 61.6±20.31 ng/ml corresponding to 142.5±44.63% and 182.5+-68.08% of basal value respectively. 5. Both early response at 1 hour and late response on 5 and 10 days after first administration of hCG become gradually neligable according to the increase of gestational weeks, the groups of 11-15 and 16-19 weeks gestation and basal hormonal levels.

In vivo antiviral activitiy of Sanguisorba officinalis roots against viral hemorrhagic septicemia virus in olive flounder Paralichthys olivaceus

Seo, JK,Do, CY,Jung, MH,Jung, SJ,Kang, SY Georg Thieme Verlag KG 2015 Planta medica Vol.81 No.16

거대자궁근종의 1례

이영주(YJ Lee),박찬영(CY Park),정경환(KH Chung),서병화(BH Shu),유훈(H Yoo),이동화(DH Rhee),강득용(DY Kang) 대한산부인과학회 1982 Obstetrics & Gynecology Science Vol.25 No.6

41세의 미산부에서 거대자궁근종을 경험하였기에 문헌고찰과 아울러 보고하는 바이다. A case of great myoma in 41 year-old nulliparous woman was presented, and a briefly review on literature was noticed.

무월경 32 주에 동반된 복강임신 1 예

김승보,진규섭,허주엽,홍진기,강인석 대한산부인과학회 1995 Obstetrics & Gynecology Science Vol.38 No.5

저자들은 임신 32주까지 지속된 복강임신에서 제왕절개술 후 생존한 1.9kg의 건강한 아이를 분만하였기에 이에 간단한 문헌 고찰과 함께 보고하는 바이다. A pregnancy in which the conceptus develops on the peritoneal surfaces of the abdomen is termed an abdominal pregnancy. Approximately 0.03% of all ectopic pregnancy are abdominal pregnancy. Early diagnosis is of the utmost importance in light of the maternal and perinatal mortaliy. We report a case of abdominal pregnancy with a review of literature.

Chitosan nanoparticles for 5-aminolevulinic acid based photodynamic therapy

Chung-Wook Chung,Young-IL Jeong,Cy-Hyun Kim,Do-Hyung Kim,Hye-Myung Lee,Tae-Won Kwak,Dae-Hwan Kang 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

Photodynamic therapy (PDT) is a palliative therapy for treatment of unresectable tumor and has been used to cure various kind of tumors. Recently, 5-aminolevulinic acid (ALA) has been used as a pro- photosensitizer, which can be transferred to intercellular protoporphyrin IX (PpIX), which is a strong photosensitizer, via the heme pathway. The main limitation of using ALA in PDT is the hydrophilic properties of ALA, which results in low cellular uptake. Chitosan is known to enhance mucosal delivery drugs and to enhance uptake of anticancer agent into tumor cells In this study, poly(ethylene glycol)-conjugated chitosan was synthesized and ALA-incorporated nanoparticle (CNP-ALA) was prepared for delivery of photosensitizer into human cholangiocarcinoma cells. CT26 murine colon carcinoma cells were treated with 0.1mM CNP-ALA or ALA for 24h. The effect of CNP-ALA was evaluated by monitoring the intra-converted PpIX amount and cell survival after irradiation under different light intensity. Results indicated that CNP-ALA as a nano-photosensitizer enhances intracellular PpIX generation and then cellular phototoxicity. We suggest that CNP-ALA is a promising carriers for photosensitizer to tumor cells.