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Biolistic-mediated Transformation of Lilium longiflorum cv. Nellie White
Kamo, Kathryn,Han, Bong Hee American Society for Horticultural Science 2008 HortScience Vol.43 No.6
<P>Slow-growing compact calluses were initiated from bulb scales of <I>Lilium longiflorum</I> cv. Nellie White that had been cultured for at least 6 months on Murashige and Skoog (MS) medium with 9 μm dicamba. To develop a reliable selection system, the sensitivity of nontransformed calluses and in vitro plants to different selective agents such as phosphinothricin, kanamycin, geneticin, paromomycin, and hygromycin was tested when grown on MS medium. Nontransformed calluses showed high sensitivity to 0.5 mg·L<SUP>−1</SUP> phosphinothricin, 25 mg·L<SUP>−1</SUP> geneticin, and 5 mg·L<SUP>−1</SUP> hygromycin. Nontransformed plants grown in vitro died on either 2 mg·L<SUP>−1</SUP> phosphinothricin or 75 mg·L<SUP>−1</SUP> hygromycin. Plants did not die when grown on either 200 mg·L<SUP>−1</SUP> kanamycin or 100 mg·L<SUP>−1</SUP> geneticin, and 100 mg·L<SUP>−1</SUP> paromomycin stimulated plant growth. Transformation was achieved using biolistics on callus bombarded with either the <I>bar-uidA</I> fusion gene under control of the CaMV 35S promoter or <I>npt II</I> and <I>uidA</I> under control of the CaMV 35S promoter. One week after biolistic bombardment, callus bombarded with the <I>bar-uidA</I> fusion gene was cultured for 1 month on MS medium supplemented with 9 μm dicamba and 0.1 mg·L<SUP>−1</SUP> phosphinothricin and then transferred to 0.2 mg·L<SUP>−1</SUP> phosphinothricin for 1 month followed by 1.0 mg·L<SUP>−1</SUP> for the next 4 months. Regenerating shoots and well-established plants were cultured on MS medium lacking hormones and with either 0.2 mg·L<SUP>−1</SUP> or 2.0 mg·L<SUP>−1</SUP> phosphinothricin, respectively. Callus bombarded with the <I>npt II</I> gene was cultured on MS medium with 50 mg·L<SUP>−1</SUP> geneticin until shoots regenerated. Regenerated shoots were cultured on MS medium lacking hormones. Under optimal conditions, 10 transgenic plants were selected from seven plates of callus bombarded with the <I>bar-uidA</I> fusion gene using phosphinothricin for selection. Both Southern hybridization of genomic DNA and polymerase chain reaction analysis verified the presence of the transgene in transformed ‘Nellie White’ plants. Transgenic plants were phenotypically normal, and they were crossed with nontransformed plants of <I>L. longiflorum</I> cvs. Sakai, Yin tung, Sakai, and Flavo. The presence of the <I>bar</I> gene in 41% of the T1 progeny plants confirmed stable integration of the transgene into the genomic DNA of transgenic lily plants. β-glucuronidase expression resulting from the <I>uidA</I> gene was demonstrated in leaves and roots of some of the transgenic lily plants by histochemical staining, determination of the specific activity of the β-glucuronidase enzyme, and Northern hybridization.</P>
Kamo, Ken-Ichi,Yanagihara, Hirokazu,Kato, Akio,Yoshimoto, Atsushi Institute of Forest Science 2008 Journal of Forest Science Vol.24 No.3
In this paper, we apply a logistic regression model to the data of snow damage on sugi (Cryptomeria japonica) occurred in Toyama prefecture (in Japan) in 2004 for estimating the risk probability. In order to specify the factors effecting snow damage, we apply a model selection procedure determining optimal subset of explanatory variables. In this process we consider the following 3 information criteria, 1) Akaike's information criterion, 2) Baysian information criterion, 3) Bias-corrected Akaike's information criterion. For the selected variables, we give a proper interpretation from the viewpoint of natural disaster.
Photochemistry of pharaonis phoborhodopsin and its interaction with the transducer
Kamo, Naoki,Shimono, Kazumi,Iwamoto, Masayuki,Sudo, Yuki,Yoshida, Hideaki Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2
Phoborhodopsin (pR or sensory rhodopsin II, sRII; the absorption maximum of ∼ 500 nm) is a retinoid protein and works as a photoreceptor of the negative phototaxis of Halobacterium salinarum. pharaonis phoborhodopsin (ppR or pharaonis sensory rhodopsin II, psRII) is a corresponding protein of Natronobacterium pharaonis. These sensory proteins form a complex with a cognate transducer protein in the membrane, and this complex transmits the light-signal to the cytoplasm to evoke avoidance reaction from blue-green light. Recently, the functional expression in Escherichia coli membrane of ppR was achieved, which can afford a large amount of the protein and enables mutant studies to clarify the role of various amino acid residues. A truncated transducer which can bind to ppR is also expressed in Escherichia. coli membrane. In this article, we will review properties of ppR mainly using observations of our laboratory; which contains photochemistry (photocycle), light-driven proton uptake, release and transport, F -helix titling during photocycle and association of the transducer.
Kenta Kamo,Hidehiko Kido,Satoshi Kido 대한고관절학회 2019 Hip and Pelvis Vol.31 No.1
Purpose: The difficulty of femoral preparation with supine-position hip hemi-arthroplasty (HA) often leads to intra-operative fractures (IOFs). We aimed to clarify the incidence and types of IOFs in HA for hip fractures performed in the supine and lateral positions. Materials and Methods: We retrospectively investigated cases of HA for acute femoral neck fractures from June 2013 to May 2018. We examined the incidence and types of IOFs according to different approaches. We defined supine-position in HA as the supine and hip-hyperextended (over-range) femoral preparation position, and lateral position as the lateral and hip-flexed femoral preparation position. We used a short tapered wedged stem. Results: Supine-position HA was used in 46 patients (23.7%) and lateral-position HA in 148 patients (76.3%). IOFs in supine-position HA occurred in 8 patients (17.4%) and included five Vancouver AGT and three Vancouver B2 fractures. IOFs in lateral-position HA occurred in 3 patients (2.0%) and included one Vancouver AGT and two Vancouver B fractures. Supine-position HA was a risk factor for IOFs (adjusted odds ratio, 9.71; 95% confidence interval, 2.37-39.8; P<0.01) Conclusion: Supine-position in HA is an IOF risk factor and significantly increases the incidence of great trochanter fractures of Vancouver type A.
Novel Concept of a Heart-Gut Axis in the Pathophysiology of Heart Failure
Takehiro Kamo,Hiroshi Akazawa,Jun ichi suzuki,Issei Komuro 대한심장학회 2017 Korean Circulation Journal Vol.47 No.5
Patients with heart failure (HF) have structural and functional changes of the gut as a result of microcirculatory disturbances. A disrupted gut epithelial barrier may lead to translocation of microbial products into systemic circulation, possibly aggravating HF by inducing inflammatory responses. Gut microbiota play an essential role in the maintenance of host homeostasis because large quantities of their gene products complement host physiological processes. Emerging evidence has suggested the potential clinical significance of gut microbiota in the pathophysiology of HF. Imbalances of gut microbe-derived metabolites can contribute to cardiac dysfunction and other morbidities in patients with HF. Therapeutic research for HF through targeting microbiota is under way. Thus, the novel concept of a heart-gut axis may lead to breakthroughs in the development of innovative diagnostics and therapeutic approaches for HF.