Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs

Jin, H.J.,Kim, I.C.,Wee, M.S.,Yeon, S.H.,Kim, C.D.,Lee, S.S.,Cho, C.Y.,Cho, S.R.,Son, D.S.,Park, C.K. 韓國受精卵移植學會 2007 한국동물생명공학회지 Vol.22 No.4

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire (p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs

H. J. Jin,I. C. Kim,M. S. Wee,S. H. Yeon,C. D. Kim,S. S. Lee,C. Y. Cho,S. R. Cho,D. S. Son,C. K. Park,Z. D. Li 사단법인 한국동물생명공학회 2007 한국동물생명공학회지 Vol.22 No.4

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire(p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

Runx3 is required for the differentiation of lung epithelial cells and suppression of lung cancer

Lee, K-S,Lee, Y-S,Lee, J-M,Ito, K,Cinghu, S,Kim, J-H,Jang, J-W,Li, Y-H,Goh, Y-M,Chi, X-Z,Wee, H,Lee, H-W,Hosoya, A,Chung, J-H,Jang, J-J,Kundu, J K,Surh, Y-J,Kim, W-J,Ito, Y,Jung, H-S,Bae, S-C Macmillan Publishers Limited 2010 Oncogene Vol.29 No.23

Human lung adenocarcinoma, the most prevalent form of lung cancer, is characterized by many molecular abnormalities. K-ras mutations are associated with the initiation of lung adenocarcinomas, but K-ras-independent mechanisms may also initiate lung tumors. Here, we find that the runt-related transcription factor Runx3 is essential for normal murine lung development and is a tumor suppressor that prevents lung adenocarcinoma. Runx3−/− mice, which die soon after birth, exhibit alveolar hyperplasia. Importantly, Runx3−/− bronchioli exhibit impaired differentiation, as evidenced by the accumulation of epithelial cells containing specific markers for both alveolar (that is SP-B) and bronchiolar (that is CC10) lineages. Runx3−/− epithelial cells also express Bmi1, which supports self-renewal of stem cells. Lung adenomas spontaneously develop in aging Runx3+/− mice (∼18 months after birth) and invariably exhibit reduced levels of Runx3. As K-ras mutations are very rare in these adenomas, Runx3+/− mice provide an animal model for lung tumorigenesis that recapitulates the preneoplastic stage of human lung adenocarcinoma development, which is independent of K-Ras mutation. We conclude that Runx3 is essential for lung epithelial cell differentiation, and that downregulation of Runx3 is causally linked to the preneoplastic stage of lung adenocarcinoma.

Association between HSP70 Genotypes and Oocytes Development on In vitro Maturation/Fertilization in Pig

Wee, M.S.,Park, C.K.,Cho, S.R.,Lee, S.S.,Yeon, S.H.,Kim, C.D.,Cho, C.Y.,Choi, S.H.,Sang, B.D.,Son, D.S.,Li, Z.D.,Jin, H.J. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.10

This study was performed to clarify whether the variation of stress related heat shock protein 70 (HSP70) (GenBank X68213) gene was associated with the nuclear morphological change of in vitro maturation and in vitro capacitation in oocytes of pig ovaries obtained at the slaughterhouse. The nucleic acid substitution of C to G at the 483rd position was found out in HSP70 K1 (290-512) from X68213. The ovaries were categorized into CC, CG, and GG genotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) (BsiHKA I). After the second in vitro maturation of immature fresh oocytes, the relation of nuclear morphological change in oocytes with the genotype of HSP70 K1 gene was such that the MII ratios of the genotype GG and CG (46.93% and 42.20%, respectively) were significantly higher than that of the CC genotype (10.71%) (p<0.05). With respect to in vitro maturation of frozen-thawed oocytes by an open pulled straw (OPS) method, the percentage of oocytes matured to MII stage of the CG genotype showed a higher trend than CC and GG genotypes. After the in vitro maturation of immature fresh oocytes and frozen-thawed oocytes by the OPS method, the relation of the pronuclei change in oocytes matured in vitro with HSP70 genotype was assessed, and the result showed that the enlarged sperm heads (ESH) of matured fresh oocytes and frozen-thawed oocytes were 80.0% and 60.0% in the CC genotype, respectively. The CC genotype group had a significantly higher rate of ESH than the CG and the GG genotype group (p<0.05). The ratios of polyspermic invasion were not different among HSP70 of the three genotypes. It was considered that the rate of in vitro maturation of fertilized oocytes was expected to differ according to genotype of the stress related gene.

L-ascorbic acid와 α-tocophero1이 돼지정액 성상과 체외수정란의 배발달율에 미치는 영향

박희진,김은국,위갑인,강만종,김광현,김재홍,문승주 全南大學校 農業科學技術硏究所 2001 農業科學技術硏究 Vol.36 No.-

The objective of this studys were to investigate the effect of α-tocopherol and L-ascorbic acid supplement on the boar semen characteristics and in vitro development rate of porcine oocytes. Sperm viability rate was significantly higher in α-tocopherol supplement than in control(66.6%) after 72hr of storage(P<0.05). Sperm motility was increased in treatment(80%, 93.3%, 85%) than in control(73.3%) at 12h of storage(P<0.05). Following insemination with 60h of store, morular and blastocyst on in vitro development resulted in significantly higher than control(P<0.05). After 72h of semen storage L-ascorbic addition also increased the sperm viability rate(P<0.05). Treatment as sperm motility was significantly higher than control in 72h of store(60% vs 66.6%, p<0.05). Following insemination with 60h of store treated porcine oocytes on in vitro development was increased more than control(56.8%), 59.9%, 62.8% vs 45.1%, P<0.05). Morular and blastocyst on in vitro development resulted in significantly higher than control(27.1%, 30.0%, 33.1% vs 20.0%, P<0.05). The results from this experiment indicated that α-tocopherol and L-ascorbic acid supplementation, for viability and motility in porcine semen, increased delay storing time of semen and in vitro development rate following insemination.

妊娠性 高血壓時 生化學的 腎機能 檢査에 關한 考察

위황규 全南大醫大 1980 전남의대학술지 Vol.17 No.2

Prostaglandin F₂ alpha의 羊膜外注入에 依한 中期姙娠中絶

위황규 全南大醫大 1979 전남의대학술지 Vol.16 No.2

Osteovit가 가토의 두개골 결손부 치유과정에 미치는 영향

위성신,한기택,임풍,조성필 大韓成形外科學會 1989 Archives of Plastic Surgery Vol.16 No.2

For many years, surgeons, bioengineers and related biomedical personnel have been striving to find a suitable material for the replacement of lost bone. Recently, Osteovit (purified collagen matrix of bovine origin, B. Braun, Melsungen AG) has been developed as a useful material for bone substitute. Implanted collagen material is absorbed itself by the enzymatic activity and phagocytosis of host tissue, and promotes the early ingrowth of vascularity and cell producing elements, and then induces the early healing of defects and new bone formation. The present study was designed to inveatigate the effect of Osteovit on the osteogenesis at the calvarial bone defect and to compare the osteogenetic effect of Osteovit with that of control and autogenous bone graft groups. In 25 rabbits, three full thickness bony defects measuring about 1 cm in diameter were made on the parietal bone of each rabbit and these bony defects were treated as followings and divided into three groups: 1) one hole as non-treated control group, 2) another hole as autogenous tibial bone grafted group and 3) the third hole as Osteovit treated group. Radiographic, macroscopic and histologic examinations were performed in 5 rabbits at 1, 2, 4, 8 and 12 weeks respectively after the operation. The results were as follows; 1. The control group began osteoid formation at 2 weeks. The new bone formation and initiation of lamellation was found at 4 weeks. At 8 weeks, the bone defect site was replaced partially by calcified and lamellated new bone. And then newly formed bone was converted into thin trabecular bone at 12 weeks. 2. The bone regeneration in the autogenous bone graft group showed more rapid and better than that in control and Osteovit treated groups. The newly formed bone was mainly compsoed of thick compact bone, like the surrounding normal cortical bone at 12 weeks. 3. In the early stage of Osteovit treated group, the osteoid formation and calcification were not rapid in comparison with that of bone graft group. But in the late stage, the extensive formation of osteoid, calcification and lamellae could be observed as a result of progressive absorption of Osteovit and on significant difference was found in healing rate between the Osteovit treated and bone graft groups. In summary, Osteovit promotes good bony regeneration comparable to autogenous bone graft when it is used in bone defect instead of bone graft, and can be used as a bone substitute.

반흔성 하안검 외반증에 대한 Composite Graft의 치험예

함기선,위성신,조해석,임풍,조문제 大韓成形外科學會 1980 Archives of Plastic Surgery Vol.7 No.1

Eyelid ectropion was the most common late sequelae encountered in our experiences following trauma or thermal burn around the eyelid. Those deformities must be corrected early to preserve visual acuity. Split-skin graft to the upper eyelid and full thickness skin graft to the lower lid have been emphasized to the most reliable methods for correction of the cicatrical ectropion. However, there is tendency toward contracture under these grafts, and the ectropion recur and reoperation or additional sugery required. To solve these problems, we have corrected the cicatrical ectropion with free composite graft of skin and cartilage from the concha of the auricle. The results of these composite grafts are favorable without recurrence.

TiAl 합금의 내산화성 개선을 위한 Al-21Ti-23Cr 플라즈마 용사코팅

李在敬,吳明勳,李炯根,韋當文 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.10

To improve the oxidation resistance of γ-based TiAl alloy, Al-Ti-Cr coatings of approximately 150 ㎛ thickness were deposited on Ti-48Al alloy using an air plasma spraying(APS) process. We used Al-Ti-Cr powders with the composition of Al-2lTi-23Cr(at.%). Isothermal and cyclic oxidation tests at 1000℃ in air were utilized to investigate the oxidation resistance of both Al-Ti-Cr coatings and of TiAl alloys with Al-Ti-Cr coatings. Phase identification of Al-Ti-Cr coatings was performed by XRD. After exposure at 1000℃ in air, rutile TiO_2, which resulted from the oxidation of Ti during spraying, was always found in addition to protective α-Al_2O_3 in the oxide scale. However, isothermal and cyclic oxidation tests of a Ti-48Al alloy coated with an Al-Ti-Cr coating showed that this coating improved the oxidation resistance of TiAl alloy at 1000℃ despite partial spalling in the Al-Ti-Cr coating layer. Moreover, cyclic oxidation tests of Al-Ti-Cr coatings showed stable oxidation behavior at 1000℃ up to 100h because of good adhesion between the coating layer and the oxide scale although the growth rate of oxide was relatively high. Therefore, it could be suggested that the Al-Ti-Cr coating was effective in enhancing the oxidation resistance of the TiAl alloy at 1000℃, although the improvement of adhesion between the coating layer and the TiAl substrate was required.