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K. Seetha Ram,K. Satish Babu,G. Tabitha,K. Rajeshwari,G. Jaya Lakshmi,B. Boje Gowd,J. B. Peravali,M. Subba Rao,P. Venugopala Rao 보안공학연구지원센터 2015 International Journal of Bio-Science and Bio-Techn Vol.7 No.6
Most of the bacterial and other simple non glycosylated recombinant proteins were conventionally produced from IPTG inducible Escherichia coli BL21(DE3). Considering the factors like cost and toxic nature of IPTG, as an alternative, salt inducible Escherichia coli GJ1158 was used in this study for the over production of staphylokinase variant (sak – hirulog) using fed batch fermentation, cost effectively. Optimization of physico chemical factors viz., dissolved oxygen (DO), carbon, nitrogen and phosphate sources on bacterial growth for the production of recombinant sak – hirulog using batch and fed batch fermentation was studied. In batch culture, increased DO at more than 30 % did not influence the enhanced expression of sak – hirulog, but significant improvement was observed in fed batch cultivation. Supplementation of production medium with different nutrient sources like dextrose, yeast extract and dipotassium hydrogen phosphate (K2HPO4) enhanced the sak – hirulog expression in fed batch fermentation process even without disturbing the cell growth by providing 50 % DO. Approximately 1178 mg/L of specific protein was obtained using cost effective modified glucose yeast exgtract (GYE) media devoid of sodium chloride. This study also reports the highest concentration of recombinant protein from salt inducible expression host till to date, which manages to satisfy the production of bifunctional staphaphylokinase variant using economically feasible bacterial expression host Escherichia coli GJ1158.
Reddy, Venu,Satish Babu, K.K.C.,Torati, Sri Ramulu,Eom, Yun Ji,Trung, Tran Quang,Lee, Nae-Eung,Kim, CheolGi Elsevier 2018 Journal of industrial and engineering chemistry Vol.63 No.-
<P><B>Abstract</B></P> <P>The development of environmentally benign, low-processing and low-cost approaches to the large-scale preparation of advanced nanomaterials based on the use of biological materials is currently attracting great interest. Here, we report the discovery that aqueous honey solutions reduce graphene oxide in a low-cost and an eco-friendly manner, yielding highly water dispersive functionalized reduced graphene sheets. The roles of honey in the reduction of graphene oxide of as-prepared graphene are demonstrated. The possible mechanism for the de-epoxidation of graphene oxide is elucidated. The fabricated a honey-reduced graphene oxide-based field-effect transistor exhibited ambipolar transfer characteristics, thereby demonstrating that the developed material may therefore have applications in electronic devices and sensors.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Venu Reddy,K.K.C. Satish Babu,Sri Ramulu Torati,엄윤지,TranQuangTrung,이내응,김철기 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.63 No.-
The development of environmentally benign, low-processing and low-cost approaches to the large-scale preparation of advanced nanomaterials based on the use of biological materials is currently attracting great interest. Here, we report the discovery that aqueous honey solutions reduce graphene oxide in a low-cost and an eco-friendly manner, yielding highly water dispersive functionalized reduced graphene sheets. The roles of honey in the reduction of graphene oxide of as-prepared graphene are demonstrated. The possible mechanism for the de-epoxidation of graphene oxide is elucidated. The fabricated a honey-reduced graphene oxide-based field-effect transistor exhibited ambipolar transfer characteristics, thereby demonstrating that the developed material may therefore have applications in electronic devices and sensors.