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Label-free Microarray-based Binding Affinity Constant Measurement with Modified Fluidic Arrangement
junwei Hu,Ru Chen,Chenggang Zhu,Bilin Ge,Xiangdong Zhu,Lan Mi,Jiong Ma,Caiqin Han,Hao Chen,Yiyan Fei 한국바이오칩학회 2018 BioChip Journal Vol.12 No.1
The oblique-incidence reflectivity difference (OI-RD) scanning microscopy has the capability of simultaneously measuring binding curves of a protein probe with tens of thousands molecular targets in a microarray and yielding reaction rate constants. However, the quality of reaction rate constants is influenced by the fluidic system. To improve the quality of reaction rate constant measurement over the entire microarray, we demonstrate a fluidic chamber that allows the fluid to flow from the bottom to the top uniformly across the microarray and thus provides more uniform and accurate measurements of reaction rate constants with simplified fluidic design.
Chen, Mingqing,Wei, Hongbo,Cao, JunWei,Liu, Ruijie,Wang, Youliang,Zheng, Congyi Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.3
Proline accumulation has been shown to correlate with tolerance to drought and salt stresses in plants. We attempt to introduce the wild-type, mutant, and fusion proBA genes derived from Bacillus subtilis into Arabidopsis thaliana under the control of a strong promoter cauliflower mosaic virus 35S (CaMV35S). The transgenic plants produced higher level of free proline than control and the overproduction of proline resulted in the increased tolerance to osmotic stress in transgenic plants. Besides, the mutation in proBA genes, which were proved to lead $\alpha$-glutamyl kinase ($\alpha$-GK) reduces sensitivity to the end-product inhibition and the fusion of proB and proA also result in increasing proline production and confer osmotolerance in transgenic lines.
Influence of post-pouring joint on long-term performance of steel-concrete composite beam
Dunwen Huang,Junwei Han,Xiaochun Liu,Shizhuo Zhang,Tao Chen 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.28 No.1
The concrete bridge decks are usually precast and in-situ assembled with steel girders with post-pouring joint in the construction practice of super-wide steel-concrete composite beam. But the difference of concrete age between the precast slabs and the post-pouring joint has been not yet considered for the long-term performance analysis of this kind composite beam. A simply supported precast-assembled T-shaped beam was taken as an example to analyze the long-term performance of steel-concrete composite beam with post-pouring joint. Based on the deformation coordination conditions of the old-new concrete deck and steel girder, a theoretical model for the long-term behavior of precast-assembled composite beam is proposed in this paper according to age-adjusted effective modulus method. Then, the feasibility of the proposed model is verified by the available test data from the Gilbert's composite beams. Parametric studies were preformed to evaluate the influences of the cross-sectional area ratio of the post-pouring joint to the whole bridge deck, as well as the difference of concrete age between the precast slabs and the post-pouring joint, on the long-term performance of the composite beam. The results indicate that the traditional method without considering the age difference would seriously underestimate the effect of creep and shrinkage of concrete bridge decks. The concrete age difference between the precast slabs and the post-pouring joint should be demonstrated for the life cycle design and long-term performance analysis of precast-assembled steel-concrete composite beams.
Workspace and Structural Parameter Analysis for a Novel 3-PRS Parallel Mechanism
Fuwei Sun,Junwei Zhao,Guoqiang Chen 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.12
The working space is one of the important indexes to measure the working capability of parallel mechanism. In this paper, the working space of a new type of 3-PRS parallel mechanism is studied. In the beginning, a novel 3-PRS parallel mechanism is proposed with the kinematics analyzed, and then the working space and volume are obtained by search algorithm, and the working space is drawn using MATLAB. Finally, the influence of the structural parameters on the working space is discussed, which provide theoretical basis for parameter optimization.
Fast numerical simulation of sheet metal forming using the program QuickForm
Yidong Bao,Junwei Chen,Zhiguo Wang,Wenliang Chen,Wing Lee 한국소성가공학회 2010 기타자료 Vol.2010 No.6
The fast numerical simulation system QuickForm has been self-developed specially for sheet metal forming with complex geometries. In order to resolve the convergence problem of static implicit method due to the change of contact state, the uncoupling solution method of the non-linear differential equations is used in QucikForm program. The bending effect and drawing effect during forming procedure are treated into two separate processes in QuickForm system, so this uncoupling solution has two basic steps. Firstly an approximate method named pre-stressed membrane element is used to achieve the intermediate configuration of sheet in this incremental step before incremental step iteration. The sheet shape of intermediate configuration depends on the current tooling position. Secondly the nonlinear material flow within this intermediate configuration is carried out, which means the nodes of deformed sheet can only slide on this form. The highly efficient iterative solver can be used to the solution of linear equations since the improved conditioning of the linear equations resulting from the uncoupling solution. Numerical simulation results of complex parts demonstrate the higher efficiency and accuracy of the proposed special purpose program.
Metal Adsorbate-Induced Plasmon Damping in Gold Nanorods: The Difference Between Metals
Pengyu Xu,Xuxing Lu,Junwei Zhao,Yue Li,Sheng Chen,Junfei Xue,Weihui Ou,Song Han,YAPING DING,Weihai Ni 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.9
We presented a single particle study on the metal adsorbate-induced plasmon damping in Au nanorods (AuNRs) through adsorbing clusters of different metals including Pt, Au and Ag. AuNRs with different longitudinal surface plasmon resonance (LSPR) wavelength were measured and investigated individually. Linewidth broadening, plasmon shift and reduction of plasmonic resonance of single AuNRs were studied and compared between Pt, Au and Ag adsorbates. The measured linewidths perfectly match the theoretical predictions of the billiard model with increased scattering coefficients resulted from the metal adsorbates. The results indicate that the plasmon damping in case of Ag is significantly weaker than Pt and Au, which can be attributed to longer relaxation time of free electrons in Ag and therefore less loss of the oscillating plasmon electrons. In contrast to the red shift observed from Au and Pt, blue shift of the LSPR is observed in case of Ag. It suggests that plasmonic properties brought by the metal adsorbates can exert dramatic influence on the nanoparticle that is adsorbed with. We believe that our study not only provides important understanding on plasmon damping but pave the road for the fabrication of complex nanostructures with two or more metal elements.
Evaluation of energy correction algorithm for signals of PET in heavy-ion cancer therapy device
Niu, Xiaoyang,Yan, Junwei,Wang, Xiaohui,Yang, Haibo,Ke, Lingyun,Chen, Jinda,Du, Chengming,Zhang, Xiuling,Zhao, Chengxin,Kong, Jie,Su, Hong Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.1
In order to solve the contradiction between requirements of high sampling rate for acquiring accurate energy information of pulses and large amount of data to be processed timely, the method with an algorithm to correct errors caused by reducing the sampling rate is normally used in front-end read-out system, which is conductive to extract accurate energy information from digitized waveform of pulse. The functions and effects of algorithms, which mainly include polynomial fitting with different fitting times, double exponential function fitting under different sampling modes, and integral area algorithm, are analyzed and evaluated, and some meaningful results is presented in this paper. The algorithm described in the paper has been used preliminarily in a prototype system of Positron Emission Tomography (PET) for heavy-ion cancer therapy facility.
A real-time sorting algorithm for in-beam PET of heavy-ion cancer therapy device
Ke, Lingyun,Yan, Junwei,Chen, Jinda,Wang, Changxin,Zhang, Xiuling,Du, Chengming,Hu, Minchi,Yang, Zuoqiao,Xu, Jiapeng,Qian, Yi,She, Qianshun,Yang, Haibo,Zhao, Hongyun,Pu, Tianlei,Pei, Changxu,Su, Hong Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.10
A real-time digital time-stamp sorting algorithm used in the In-Beam positron emission tomography (In-Beam PET) is presented. The algorithm is operated in the field programmable gate array (FPGA) and a small amount of registers, MUX and memory cells are used. It is developed for sorting the data of annihilation event from front-end circuits, so as to identify the coincidence events efficiently in a large amount of data. In the In-Beam PET, each annihilation event is detected by the detector array and digitized by the analog to digital converter (ADC) in Data Acquisition Unit (DAQU), with a resolution of 14 bits and sampling rate of 50 MS/s. Test and preliminary operation have been implemented, it can perform a sorting operation under the event count rate up to 1 MHz per channel, and support four channels in total, count rate up to 4 MHz. The performance of this algorithm has been verified by pulse generator and <sup>22</sup>Na radiation source, which can sort the events with chaotic order into chronological order completely. The application of this algorithm provides not only an efficient solution for selection of coincidence events, but also a design of electronic circuit with a small-scale structure.
Piezo channels in the urinary system
Li Xu,Hu Junwei,Zhao Xuedan,Li Juanjuan,Chen Yuelai 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
The Piezo channel family, including Piezo1 and Piezo2, includes essential mechanosensitive transduction molecules in mammals. Functioning in the conversion of mechanical signals to biological signals to regulate a plethora of physiological processes, Piezo channels, which have a unique homotrimeric three-blade propeller-shaped structure, utilize a cap-motion and plug-and-latch mechanism to gate their ion-conducting pathways. Piezo channels have a wide range of biological roles in various human systems, both in vitro and in vivo. Currently, there is a lack of comprehensive understanding of their antagonists and agonists, and therefore further investigation is needed. Remarkably, increasingly compelling evidence demonstrates that Piezo channel function in the urinary system is important. This review article systematically summarizes the existing evidence of the importance of Piezo channels, including protein structure, mechanogating mechanisms, and pharmacological characteristics, with a particular focus on their physiological and pathophysiological roles in the urinary system. Collectively, this review aims to provide a direction for future clinical applications in urinary system diseases.