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Carotenoid Content and Biosynthesis Genes in Orange-Colored Chinese Cabbage
Seohee Lee,Sang-Choon Lee,Dong Hae Byun,Jae Kwang Kim,Jee Young Park,Jong hoon Lee,Hyejoon Joo,Junki Lee,Murukarthick Jayakodi,Tae-Jin Yang 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Chinese cabbage (Brassica rapa ssp. pekinensis) is one of the most important vegetables and widely cultivated in Asia countries including Korea and China. Recently, whole genome sequence and full-length cDNA information of this species became available, which are encouraging genetic studies of this species to characterize agricultural important traits. Orange-colored (Or) cultivar of Chinese cabbage has inner leaves in orange, whereas other cultivars generally cultivated have yellow (Ye)- or white-colored inner leaves. In this study, we investigated phenotypes and carotenoid biosynthesis genes related to color variation in the Or cultivar. Firstly we compared the carotenoid content and composition between the Or and Ye cultivars by HPLC analysis. The inner leaves of Or cultivar contained approximately 9-fold high β-carotene content, whereas content of both lutein and violaxanthin was decreased to less than 30%, compared to Ye cultivar. Or cultivar was segregated with ratio of 3:1 in F2 population derived from crossing between Or and Ye inbred lines, indicating that Or phenotype is controlled by single recessive gene. To identify this gene, we investigated the expression of several genes involved in carotenoid biosynthesis by RT-PCR analysis. Among genes tested, two encoding putative carotenoid isomerase (CRTISO) and phytoene desaturase (PDS) were identified to show different expression between Or and Ye cultivars. Through further analysis of genomic DNA regions of these two genes, we could expect that several mutations such as InDel and base-substitution occurred and then affected expression of these genes in Or cultivar. In this presentation, I will introduce more detailed results for Or cultivars.
Jonghoon Lee,Junki Lee,Jee Young Park,Tae-Jin Yang 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Chloroplasts are plant-specific organelles, which have their own genome. Most of the plant chloroplast genomes (CP genome) are highly conserved in terms of its gene contents and genome structures, and they exist in cells with abundant copy numbers. Because of numerous copy numbers, the complete chloroplast sequence assembly pipeline with small amount of whole genome resequencing data, produced by NGS technique, was established in our laboratory. From 14 accessions of cabbage (Brassica oleracea L.) resequencing data produced by Illumina Hi-seq 2000, CP genomes were assembled and compared to each other. 18 sequence variance regions were detected, and 6 HRM(High Resolution Melting curves) markers were developed. Approximately 1 Gb of whole genome sequencing data of 10 Brassica rapa and 2 Brassica napus were also obtained from Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science. With these resequencing data, all CP genomes from these accessions were assembled. Total 27 complete CP genomes of B.oleracea, B.rapa, B.napus, and brassico-raphanus which is a novel allotetraploid species between B.rapa and Raphanus sativus, were compared in sequence level. Phylogenetic analysis based on the comparison revealed that B.rapa could be the maternal species when rapeseeds and brassico-raphanus became allotetraploid species. Additionally, CP genome of B.napus cv.M083 is closer to B.rapa accessions than the other B.napus accessions, thus B.napus could have several different origins.
( Junki Lee ),( Jaekyung Chon ),( Jongsung Lim ),( Eun-kyoung Kim ),( Gyoungju Nah ) 한국육종학회 2017 Plant Breeding and Biotechnology Vol.5 No.3
Chloroplast genome sequencing has served as valuable source for developing DNA markers, including the authentication of plant material used for health supplement from its fraudulent materials. We sequenced and analyzed the chloroplast genome of Allium victorialis, a medicinal plant, to discover potential marker regions for the authentication from Veratrum patulum, an inedible toxic plant. Although we examined conventional barcode marker loci in chloroplast, matK and rbcL, there was a difficulty in aligning coding regions and determining PCR primer sequences in these two loci between A. victorialis and V. patulum, possibly due to the distant evolutionary relationship. Instead, we identified potential DNA markers that carry Insertion/Deletion (InDels) that are able to discriminate these two species around clpP, petB, petD, rpl22, and ycf2 loci. In this analysis, we demonstrated the possibility of developing potential DNA markers in the chloroplast genome other than conventional barcode markers, such as matK and rbcL. The potential DNA markers identified in this analysis will serve as useful tools for future authentication of Allium and Veratrum species.
( Junki Lee ),( Ho Jun Joh ),( Nam-hoon Kim ),( Sang-choon Lee ),( Woojong Jang ),( Beom Soon Choi ),( Yeisoo Yu ),( Tae-jin Yang ) 한국육종학회 2017 Plant Breeding and Biotechnology Vol.5 No.2
Resource plants are important and have strong potential for a variety of utilities as crops or pharmaceutical materials. However, most resource plants remain wild and thus their utility for breeding and biotechnology is limited. Molecular markers are useful to initiate genetic study and molecular breeding for these understudied resource plants. We collected various wild collections of Peucedanum japonicum which is indigenous resource plants utilized as oriental medicine and leafy vegetables in Korea. In this study, we produced two independent whole genome sequences (WGSs) from two collections and identified large scale polymorphic simple sequence repeat (pSSR) based on our pipeline to develop SSR markers based on comparison of two WGSs. We identified a total of 452 candidate pSSR contigs. To confirm the accuracy and utility of pSSR, we designed ten SSR primer pairs and successfully applied those to seven collections of P. japonicum. The WGS and pSSR candidates identified in this study will be useful resource for genetic research and breeding purpose for the valuable resource plant, P. japonicum.
Automatic Lane Change System with Double Loop PID Algorithm
Junki Hong,Jaehwan Choi,Sooho Woo,Jaehong Lee,Soon-Geul Lee,Jaehyeong Kim,Kyungsu Cha 제어로봇시스템학회 2022 제어로봇시스템학회 국제학술대회 논문집 Vol.2022 No.11
For automatic lane change of the advanced driver assistance system, it is necessary to compensate the sensor limitations and also to provide a stable and accurate steering controller. In this paper, a look-ahead model that uses the future position data of the vehicle to supplement the limitations of the sensor mounted in the vehicle is used. In addition, to make smooth the lateral control of the vehicle body, a double loop PID is used to control the heading angle rate. Simulink-based program is used for the automatic lane change by manually operating the turn indicator in the vehicle. The program was simulated with IPGs CarMaker and ran in a real-world environment with MicroAutoBox II on Rav4 of Toyota. Experimental results showed that both the real vehicle test and the simulation completed the lane change stably with an error within 50 cm, despite the influence of the disturbance in the real environment.
요구사항 온톨로지 기반의 시맨틱 태깅을 활용한 산출물의 재사용성 지원을 위한 요구사항추적 방법
이준기(Junki Lee),조혜경(Hae-Kyung Cho),고인영(In-Young Ko) 한국정보과학회 2008 정보과학회논문지 : 소프트웨어 및 응용 Vol.35 No.6
산출물들의 추적 관계 정의를 이용한 요구사항 추적을 통해, 기존의 컴포넌트 자체의 재사용뿐만 아니라 컴포넌트 개발과정에서 나오는 다양한 산출물들을 요구사항 기반으로 재사용할 수 있다. 이러한 재사용성 증가를 목적으로 하는 요구사항추적을 지원하기 위해서는 산출물들이 요구사항을 기반으로 표현될 수 있어야 하고, 표현된 요구사항을 기반으로 하여 추적관계를 추론하는 메커니즘이 제공되어야 한다. 이를 위해, 본 논문에서는 시맨틱스 기반의 요구사항추적을 지원하기 위해서 요구사항 온톨로지를 하여 기술한다. 그 다음에 산출물들이 요구사항 온톨로지를 통해서 시맨틱 태깅되는 기술을 기술한다. 본 논문은 이와 같이 요구사항 추적을 위한 메커니즘을 제안하고, 요구사항 온톨로지의 구조를 정의하며 프로토타입을 제시한다. Requirements traceability enables to reuse various kinds of software artifacts, which are the results from software development life cycle, rather than reuse source code only. To support requirements traceability for reuse of software artifacts, 1) artifacts should be described based on requirements and 2) a requirements tracing method should be supported. In this paper, we provide a description model for annotating requirements information to software artifacts by using requirements ontology. We also provide semantic tagging method users to efficiently annotate artifacts with the requirements ontology. And we finally present how requirements traceability is supported based on requirements ontology and also suggest the system architecture for requirements traceability support.