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Secondary Metabolites of Miconia rubiginosa
Juliana Rodrigues,Daniel Rinaldo,Marcelo Aparecido da Silva,Lourdes Campaner dos Santos,Wagner Vilegas 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.7
The Miconia genus is the most representative of the Melastomataceae family, and some species are commonly used in Brazilian folk medicine as anti-inflammatory agents. In this work we investigated the leaves from Miconia rubiginosa (Bonpl.) DC, using high-speed countercurrent chromatography, which yielded 11 substances (eight flavonoids, gallic acid, casuarictin, and schizandriside). Identification was achieved using nuclear magnetic resonance spectroscopy and high-performance liquid chromatography–circular dichroism–diode array detection analyses.
Antimicrobial Activity of Miconia Species (Melastomataceae)
Juliana Rodrigues,Danielle Carvalho Michelin,Daniel Rinaldo,Guilherme Juli? Zocolo,Lourdes Campaner dos Santos,Wagner Vilegas,H?ida Regina Nunes Salgado 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.1
This work evaluated the antimicrobial activity of the methanol and chloroform extracts of the leaves of Mi-conia cabucu, Miconia rubiginosa, and Miconia stenostachya using the disc-diffusion method. The results obtained showedthat the methanol extracts of the leaves of M. rubiginosaand M. stenostachyaand the chloroform extract of the leaves of M.cabucu presented antimicrobial activity against the tested microorganisms.
Can a spontaneous smile invalidate facial identification by photo-anthropometry?
Pinto, Paulo Henrique Viana,Rodrigues, Caio Henrique Pinke,Rozatto, Juliana Rodrigues,da Silva, Ana Maria Bettoni Rodrigues,Bruni, Aline Thais,da Silva, Marco Antonio Moreira Rodrigues,da Silva, Ricar Korean Academy of Oral and Maxillofacial Radiology 2021 Imaging Science in Dentistry Vol.51 No.3
Purpose: Using images in the facial image comparison process poses a challenge for forensic experts due to limitations such as the presence of facial expressions. The aims of this study were to analyze how morphometric changes in the face during a spontaneous smile influence the facial image comparison process and to evaluate the reproducibility of measurements obtained by digital stereophotogrammetry in these situations. Materials and Methods: Three examiners used digital stereophotogrammetry to obtain 3-dimensional images of the faces of 10 female participants(aged between 23 and 45 years). Photographs of the participants' faces were captured with their faces at rest (group 1) and with a spontaneous smile (group 2), resulting in a total of 60 3-dimensional images. The digital stereophotogrammetry device obtained the images with a 3.5-ms capture time, which prevented undesirable movements of the participants. Linear measurements between facial landmarks were made, in units of millimeters, and the data were subjected to multivariate and univariate statistical analyses using Pirouette<sup>®</sup> version 4.5 (InfoMetrix Inc., Woodinville, WA, USA) and Microsoft Excel<sup>®</sup> (Microsoft Corp., Redmond, WA, USA), respectively. Results: The measurements that most strongly influenced the separation of the groups were related to the labial/buccal region. In general, the data showed low standard deviations, which differed by less than 10% from the measured mean values, demonstrating that the digital stereophotogrammetry technique was reproducible. Conclusion: The impact of spontaneous smiles on the facial image comparison process should be considered, and digital stereophotogrammetry provided good reproducibility.
Juliana Mendes Monteiro,Renata Estebanez Vollú,Marcia Reed Rodrigues Coelho,Celuta Sales Alviano,Arie Fitzgerald Blank,Lucy Seldin 한국미생물학회 2009 The journal of microbiology Vol.47 No.4
Molecular approaches [PCR-denaturing gradient gel electrophoresis (DGGE)] were used to determine whether three different vetiver (Chrysopogon zizanioides) genotypes, commercially used in Brazil and considered economically important over the world, select specific bacterial populations to coexist in their rhizospheres. DGGE profiles revealed that the predominant rhizospheric bacterial community hardly varies regarding the vetiver genotype. Moreover, using traditional cultivation methods, bacterial strains were isolated from the different rhizospheres. Colonies presenting different morphologies (83) were selected for determining their potential for plant growth promotion. More than half of the strains tested (57.8%) were amplified by PCR using nifH-based primers, specific for the enzyme nitrogenase reductase. The production of siderophores was observed in 88% of the strains, while the production of antimicrobial substances was detected in only 14.5% of the isolates when Micrococcus sp. was used as the indicator strain. Production of indole-3-acetic acid and the solubilization of phosphate were observed in 55.4% and 59% of the isolates, respectively. In total, 44 strains (53%) presented at least three characteristics of plant growth promotion and were submitted to amplified ribosomal DNA restriction analysis. Twenty-four genetic groups were formed at 100% similarity and one representative of each group was selected for their identification by partial 16S rRNA gene sequencing. They were affiliated with the genera Acinetobacter, Comamonas, Chryseobacterium, Klebsiella, Enterobacter, Pantoea, Dyella, Burkholderia, or Pseudomonas. These strains can be considered of great importance as possible biofertilizers in vetiver.
Impact of COVID-19 on the daily routine of radiology clinics
Juliana de Carvalho Carmelo,Tatielly Karine Costa Alves,Micheline Salim Khouri,Rafael Pereira da Mata Santos,Lizandra Gonzaga Rodrigues,Maria Eugenia Alvarez-Leite,Flávio Ricardo Manzi 대한영상치의학회 2020 Imaging Science in Dentistry Vol.50 No.3
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Evaluation of Exogenous Promoters for Use in Brachiaria brizantha Transformation
Silveira Erica Duarte,Rodrigues Julio Carlyle Macedo,Cabral Glaucia Barbosa,Leite Juliana de Almeida,Costa Sidnei Souza,Carneiro Vera Tavares de Campos The Korean Society of Plant Biotechnology 2003 Plant molecular biology and biotechnology research Vol.5 No.2
Brachiaria (Poaceae) is the most important forage genus for cattle production in Brazil. The genetic breeding of this genus is limited by the incompatibility among species, differences in ploidy level and the natural cloning of plants by apomixis (Valle and Miles 1992). However, plant regeneration via tissue culture methods and genetic engineering provide an opportunity to introduce new characteristics in plants of this genus. We have developed methods for the 'genetic modification of Brachiaria brizantha cv. Marandu via biolistic transformation. A higher number of shoots was obtained with 4 mg/L 2.4-diclorophenoxyacetic acid and 0.2 mg/L benzylaminopurine in calli induction medium and 0.1 mg/L naphtaleneacetic acid and 4.0 mg/L kinetin in shoot regeneration medium. A selection curve for mannose was determined to use phospho mannose isomerase (PMI) gene of Escherichia coli as a selection marker. Calli formation was inhibited from 5 g/L mannose, even in the presence of sucrose while calli that were formed in the presence of mannose failed to develop embryos showing that PMI gene can be used for selection of transformants of this grass. Different promoters were tested to evaluate the efficiency based on the detection of the GUS gene expression (Jefferson et al. 1987). The monocot promoters, act1-D and ubi-1, resulted in higher expression levels than dicot promoters, ubi-3 and act-2, or the CaMV35S and CVMV promoters.
Soares Renata Cristina,Rocha Juliana Schaia,da Rosa Saulo Vinicius,Gonçalves Jéssica Rodrigues da Silva Noll,Perlas Condori Priscilla Lesly,Ribeiro Ana Elisa,Moysés Samuel Jorge,Baldani Márcia 한국역학회 2021 Epidemiology and Health Vol.43 No.-
OBJECTIVES: To conduct a systematic review of coronavirus disease 2019 (COVID-19)-related biosafety guidelines for dental clinical practice in the early stage of the pandemic, focusing on quality assessment. METHODS: Electronic (via PubMed, Scopus, Web of Science, Latin American and Caribbean Health Sciences Literature database, Brazilian Library in Dentistry, and Cochrane Library) and gray literature searches were performed for documents published up to May 12, 2020. Guidelines updated until April 17, 2021 were identified. Documents were included as guidelines if they (1) consisted of a set of statements, directions, or principles presenting current or future rules or policy; (2) were developed by government agencies, institutions, organizations, or expert panels; and (3) were related to the general conduct of health care activities rather a particular condition. Two researchers, using the Appraisal of Guidelines for Research & Evaluation II, independently extracted the recommendations and evaluated the quality of the guidelines. RESULTS: Twenty-seven documents from 19 countries were included in the review. These documents presented 122 recommendations related to (1) professional biosafety; (2) patients’/companions’ safety; (3) the organization and biosafety of the physical dental facility environment; and (4) the work process in dental care. Overall, the scientific quality of the guidelines was considered low. Some recommendations presented in these guidelines would require further research to establish their effectiveness. CONCLUSIONS: We found a wide variety of biosafety guidelines for dental practice regarding COVID-19
Tuane Krupek,Bruna Juliana Wanczinski Ferrari,Maria Angelica Raffaini Covas Pereira da Silva,Christiano Rodrigues Schamber,Dennis Armando Bertolini,Marcos Luciano Bruschi,Isolde Terezinha Santos Previ 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.5
The impact of oral supplementation with an effervescent glutamine formulation on the beneficial effects of antiretroviral therapies was evaluated in people living with HIV/AIDS. For this purpose, 12 HIV/AIDS carrier patients with CD4+ T cell counts <500, and who had received the same antiretroviral therapy for at least 1 year before starting this investigation were selected. The patients were required to dissolve the effervescent glutamine formulation (supplied in sachets) in water immediately before oral ingestion (12.4 g), once a day, after lunch or after dinner during 30 days. CD4+ T cell counts, complete blood cell counts, serum cytokines, and amino acids levels were quantified; biochemical and toxicological measurements were performed. The numbers of CD4+ T cells were increased (P < .05), and the serum C-reactive protein levels decreased (P < .01) after the administration of effervescent glutamine formulation. Serum levels of interferon-gamma inducible protein-10, RANTES, and macrophage inflammatory protein-1β were decreased after the treatment with effervescent glutamine formulation. No changes were observed in the serum levels of amino acids, hematological, toxicological, and biochemical parameters. In conclusion, the treatment during 30 days with effervescent glutamine formulation was well tolerated, promoted reduction of inflammation, and improved the beneficial effects of antiretroviral therapies in HIV/AIDS carrier patients.
Laura Vicedo Jacociunas,Heloisa Helena Rodrigues de Andrade,Mauricio Lehmann,Bianca Regina Ribas de Abreu,Alexandre de Barros Falca˜o Ferraz,Juliana da Silva,Rafael Rodrigues Dihl 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.10
Cynara scolymus L. (Asteraceae), popularly known as artichoke, has been widely used in herbal medicine for the treatment of hepatic diseases. The genotoxicity of C. scolymus L. leaf extract (LE) and the ability to modulate the genetic toxicity of the alkylating agent ethyl methanesulfonate (EMS) were assessed using the comet assay on Chinese hamster ovary cells. Genotoxicity was evaluated after 1- and 24-h treatments using four different LE concentrations: 0.62, 1.25, 2.5, and 5.0 mg/mL. Antigenotoxicity was assessed for pretreatment, simultaneous treatment, and post-treatment. All doses used led to a significant increase in the frequency of DNA damage, after exposure for 1 and 24 h. In the antigenotoxicity experiments, LE reduced the frequency of DNA damage induced by EMS in the simultaneous treatment only. However, the lowest dose was more protective than higher concentrations. Flavonoids and phenolic compounds are, probably, the C. scolymus constituents responsible for its genotoxic and antigenotoxic effects
Effect of endodontic sealer on postoperative pain: a network meta-analysis
Monteiro Cynthia Maria Chaves,Martins Ana Cristina Rodrigues,Reis Alessandra,de Geus Juliana Larocca 대한치과보존학회 2023 Restorative Dentistry & Endodontics Vol.48 No.1
This systematic review and network meta-analysis aimed to answer the following focused research question: “Does the type of endodontic sealer affect the postoperative pain in patients who received endodontic treatment?” Different databases and grey literature were surveyed. Only one randomized controlled trial were included. The risk of bias in the studies was evaluated by using the Cochrane Collaboration’s tool. A random-effects meta-analysis was conducted to compare the risk and intensity of postoperative pain. The quality of the body of evidence was assessed using the Grading of Recommendations Assessment, Development, and Evaluation approach. Out of 11,601 studies, 15 remained for qualitative analyses and 12 for meta-analysis. Seven studies were classified at high risk of bias, and 8 studies raised some concerns. No significant differences between the endodontic materials were observed in the direct comparisons, both in risk and in intensity of postoperative pain (pairwise comparisons with 2 studies: I2 = 0%; p > 0.05 and 8 studies: I2 = 23%; p > 0.05, respectively). The certainty of the evidence was graded as low or moderate. There was no difference in the risk and intensity of postoperative pain after filling with different endodontic sealers. Further systematic reviews should be conducted.