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Katiuska Tuttis,Daryne Lu Maldonado Gomes da Costa,Juliana Mara Serpeloni,Lourdes Campaner dos Santos,Eliana Aparecida Varanda,Wagner Vilegas,Wilner Martınez-Lopez,Ilce Mara de Syllos Cólus 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.5
Different species of the genus Pouteria have been used in folk medicine for the treatment of inflammation, fever, ulcers, diabetes, and diarrhea. We analyzed the phytochemical profile of the hydroethanolic extract from Pouteria ramiflora leaves by electrospray ionization ion trap tandem mass spectrometry and high-performance liquid chromatography-diode array detection, and examined whether it alone and in combination with cisplatin interfered with cell proliferation and death processes in HepG2 (human hepatocellular carcinoma) and FGH (human gingival fibroblasts) cells. Five compounds were identified in the extract: gallic acid, myricetin-3-O-α-l-arabinopyranoside, quercetin-3-O-β-d-galactopyranoside, myricetin-3-O-α-l-rhamnopyranoside, and myricetin-3-O-β-d-galactopyranoside. The extract was cytotoxic to both cell lines by inducing apoptotic cell death and acted in synergy with cisplatin; such effect was stronger in HepG2 cells than in FGH cells, demonstrating some selectivity to tumor cells. In HepG2 cells, the extract exerted antiproliferative effect mediated by induction of cell cycle arrest at the S and G2/M phases. Association of the extract with cisplatin enhanced the latter's antiproliferative effect, arrested the cell cycle at the S phase by CDK2 modulation, and reduced the number of anti-cyclin D1-stained HepG2 cells. Simultaneous treatment with the extract and cisplatin increased the latter's cytotoxicity, apoptotic cell death, and BAX expression in HepG2 cells. Altogether, the results reported herein indicate that P. ramiflora extract is a possible adjuvant to cancer therapy, which can circumvent the cisplatin-mediated resistance mechanisms in cancer cells.
Antimicrobial Activity of Miconia Species (Melastomataceae)
Juliana Rodrigues,Danielle Carvalho Michelin,Daniel Rinaldo,Guilherme Juli? Zocolo,Lourdes Campaner dos Santos,Wagner Vilegas,H?ida Regina Nunes Salgado 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.1
This work evaluated the antimicrobial activity of the methanol and chloroform extracts of the leaves of Mi-conia cabucu, Miconia rubiginosa, and Miconia stenostachya using the disc-diffusion method. The results obtained showedthat the methanol extracts of the leaves of M. rubiginosaand M. stenostachyaand the chloroform extract of the leaves of M.cabucu presented antimicrobial activity against the tested microorganisms.
Secondary Metabolites of Miconia rubiginosa
Juliana Rodrigues,Daniel Rinaldo,Marcelo Aparecido da Silva,Lourdes Campaner dos Santos,Wagner Vilegas 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.7
The Miconia genus is the most representative of the Melastomataceae family, and some species are commonly used in Brazilian folk medicine as anti-inflammatory agents. In this work we investigated the leaves from Miconia rubiginosa (Bonpl.) DC, using high-speed countercurrent chromatography, which yielded 11 substances (eight flavonoids, gallic acid, casuarictin, and schizandriside). Identification was achieved using nuclear magnetic resonance spectroscopy and high-performance liquid chromatography–circular dichroism–diode array detection analyses.
Daryne L.M.G. Costa,Daniel Rinaldo,Eliana A. Varanda,Juliana F. de Sousa,Ana L.M. Nasser,Ana C.Z. Silva,Debora C. Baldoqui,Wagner Vilegas,Lourdes Campaner dos Santos 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.10
It is well known that phytotherapy has grown in popularity in recent years. Because a drug cannot be administered without ensuring its effectiveness and safety, the standardization and regulation of phytotherapeutic drugs are required by the global market and governmental authorities. This article describes a simple and reliable high-performance liquid chromatography–diode array detection analysis method for the simultaneous detection of myricetin-3-O-b-Dgalactopyranoside, myricetin-3-O-a-L-arabinopyranoside, and myricetin-3-O-a-L-rhaminopyranoside present in the hydroethanolic extract (ethanol/H2O, 7:3, v/v) of Pouteria torta. The mutagenic activity of the extract was evaluated on Salmonella typhimurium and by an in vivo micronucleus test on the peripheral blood cells of Swiss mice. The linearity, sensitivity, selectivity, repeatability, accuracy, and precision of the assay were evaluated. The analytical curves were linear and exhibited good repeatability (with a deviation of less than 5%) and demonstrated good recovery (within the 83–107% range). The results demonstrate that the hydroethanolic extract exhibited a mutagenic activity in both assays, suggesting caution in the use of this plant in folk medicine.